DETAILED ACTION
Notice of Pre-AIA or AIA Status
The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA .
Status of the claims
Claims 1, 24, 26-28, 36-37, and 65-77 are pending and examined herein to the extent that they read on the elected species (i.e. cis-abienol synthase, SEQ ID NOs: 4-5, 25-26, and 46-47).
All rejections of claims 17 and 19 are moot in light of Applicant’s cancellation of the claims.
The rejection of claims 1, 24, 26-28, 36-37, and 65, 68-69 and 72 under 35 U.S.C. 112(a) or 35 U.S.C. 112 (pre-AIA ), first paragraph, as failing to comply with the written description requirement is withdrawn in light of Applicant’s amendment of the claims.
Examiner notes that all of SEQ ID NOs: 106 to 111 and 206 to 208 have been rejoined.
Terminal Disclaimer
The terminal disclaimer filed on 17 November 2025 disclaiming the terminal portion of any patent granted on this application which would extend beyond the expiration date of US Patent No. 12,351,809 has been reviewed and is accepted. The terminal disclaimer has been recorded.
Status of the claims
The rejection of claims 24 and 26 under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA ), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention, is withdrawn in light of Applicant’s amendment of the claims.
The rejection of claims 1, 24, 26-28, 36-37, and 65-72 under 35 U.S.C. 103 as being unpatentable over Rontein et al (FR2903703A1) in view of in view of Wagner et al (US. Patent No.: 7,501,557; issued 10 March 2009) and Lewis et al (US Patent No. 9,603,335 B2; issued 29 March 2017) has been withdrawn in light of Applicant’s amendment of the claims.
The rejection of claims 1, 24, 26-28, 36-37, and 65-72 on the ground of nonstatutory double patenting as being unpatentable over claim 1-3, 5-11, and 14-18 of U.S. Patent No. 12,351,809 (hereafter ‘809) in view of Rontein et al (FR2903703A1) has been withdrawn in light of the terminal disclaimer filed by Applicant on 17 November 2025.
Double Patenting
The nonstatutory double patenting rejection is based on a judicially created doctrine grounded in public policy (a policy reflected in the statute) so as to prevent the unjustified or improper timewise extension of the “right to exclude” granted by a patent and to prevent possible harassment by multiple assignees. A nonstatutory double patenting rejection is appropriate where the conflicting claims are not identical, but at least one examined application claim is not patentably distinct from the reference claim(s) because the examined application claim is either anticipated by, or would have been obvious over, the reference claim(s). See, e.g., In re Berg, 140 F.3d 1428, 46 USPQ2d 1226 (Fed. Cir. 1998); In re Goodman, 11 F.3d 1046, 29 USPQ2d 2010 (Fed. Cir. 1993); In re Longi, 759 F.2d 887, 225 USPQ 645 (Fed. Cir. 1985); In re Van Ornum, 686 F.2d 937, 214 USPQ 761 (CCPA 1982); In re Vogel, 422 F.2d 438, 164 USPQ 619 (CCPA 1970); In re Thorington, 418 F.2d 528, 163 USPQ 644 (CCPA 1969).
A timely filed terminal disclaimer in compliance with 37 CFR 1.321(c) or 1.321(d) may be used to overcome an actual or provisional rejection based on nonstatutory double patenting provided the reference application or patent either is shown to be commonly owned with the examined application, or claims an invention made as a result of activities undertaken within the scope of a joint research agreement. See MPEP § 717.02 for applications subject to examination under the first inventor to file provisions of the AIA as explained in MPEP § 2159. See MPEP § 2146 et seq. for applications not subject to examination under the first inventor to file provisions of the AIA . A terminal disclaimer must be signed in compliance with 37 CFR 1.321(b).
The filing of a terminal disclaimer by itself is not a complete reply to a nonstatutory double patenting (NSDP) rejection. A complete reply requires that the terminal disclaimer be accompanied by a reply requesting reconsideration of the prior Office action. Even where the NSDP rejection is provisional the reply must be complete. See MPEP § 804, subsection I.B.1. For a reply to a non-final Office action, see 37 CFR 1.111(a). For a reply to final Office action, see 37 CFR 1.113(c). A request for reconsideration while not provided for in 37 CFR 1.113(c) may be filed after final for consideration. See MPEP §§ 706.07(e) and 714.13.
The USPTO Internet website contains terminal disclaimer forms which may be used. Please visit www.uspto.gov/patent/patents-forms. The actual filing date of the application in which the form is filed determines what form (e.g., PTO/SB/25, PTO/SB/26, PTO/AIA /25, or PTO/AIA /26) should be used. A web-based eTerminal Disclaimer may be filled out completely online using web-screens. An eTerminal Disclaimer that meets all requirements is auto-processed and approved immediately upon submission. For more information about eTerminal Disclaimers, refer to www.uspto.gov/patents/apply/applying-online/eterminal-disclaimer.
Claims 1, 24, 26-28, 36-37, and 65-77 are provisionally rejected on the ground of nonstatutory double patenting as being unpatentable over claim 41-56 of copending Application No. 19/225,122 (hereafter ‘122) in view of Rontein et al (FR2903703A1).
This is a provisional nonstatutory double patenting rejection.
Claims 41-60 of copending application ‘122 are broadly drawn to tobacco material from a modified tobacco plant, comprising a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a nucleotide encoding a polypeptide, wherein the promoter has 95%, 98% or 100% identity to SEQ ID NOs: 46 or 47 (claim 41-43); said tobacco material from a modified plant wherein the plant variety is flue-cured variety, a bright variety, a Burley variety, a Virginia variety, a Maryland variety, a dark variety, a Galpão variety, an Oriental variety, and a Turkish variety, and any one of a number of specific varieties (claims 52-55); the tobacco material from a modified plant of claim 41 wherein the nucleotide encoding a polypeptide encodes an amino acid with 95% or 100% sequence identity to one of SEQ ID NO: 18-26 or 64-79 (claims 44-45); the tobacco material from a modified plant of claim 41 wherein the nucleotide encoding a polypeptide has 95% or 100% sequence identity to one of SEQ ID NO: 27-35 or 48 to 63 (claims 46-47); the tobacco material from a modified plant of claim 41 wherein the polypeptide is involved in biosynthesis of at least one terpene (claim 48); wherein the at least one terpene is cis-abienol (claim 49); wherein the terpene is cis-abienol synthase ISOFORM1 or ISOFORM2 (claims 50-51); the tobacco material from a modified plant of claim 41 wherein the plant is a male sterile tobacco plant or a cytoplasmically male sterile tobacco plant (claim 56).
The instant claims are broadly drawn to modified tobacco or cannabis plants comprising a nucleic acid sequence encoding a cis-abienol synthase polypeptide operably linked to a heterologous promoter and wherein the polypeptide comprises the sequence of SEQ ID NO: 46 (i.e. cis-abienol synthase ISOFORM 1) or 47 (i.e. cis-abienol synthase ISOFORM 2) and wherein the promoter comprises a sequence with at least 99% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 106-111 and 206-208; said plant wherein the nucleic acid sequence comprises the sequence of SEQ ID NO: 4 or 5; said plant wherein the plant comprises an increased amount of at least one terpene compared to a non-modified plant, wherein the increase is at least 5% and wherein the terpene is neophytadiene. The claims are further drawn to said modified tobacco or cannabis plant wherein the plant wherein the nucleic acid sequence comprises the sequence of SEQ ID NOs: 26 or 27.
The claims are further drawn to the modified tobacco plant of claim 1, wherein the tobacco plant is selected from a flue-cured variety, a bright variety, a Burley variety, a Virginia variety, a Maryland variety, a dark variety, a Galpao variety, an Oriental variety ,and a Turkish variety; a cured tobacco product of the plant of claim 1, and wherein the curing is flue curing, air curing, fire, curing, or sun curing and the plant of claim 1, wherein the plant is a male sterile tobacco plant or a cytoplasmically male sterile tobacco plant.
Instant SEQ ID NOs: 207 and 208 have 100% identity to the trichome specific promoters encoded by SEQ ID NOs: 46 and 47 of copending application ‘122 (see Specification of ‘122, Examples 3-6 for disclosures relating to promoter specificity):
RESULT 1
US-19-225-122-46
Query Match 100.0%; Score 1500; DB 1; Length 1500;
Best Local Similarity 100.0%;
Matches 1500; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 AATGTTTACAATTTTAACATGTCGATTATTTTCAATTACTAATTTTTTGAAAAAAAAAAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 AATGTTTACAATTTTAACATGTCGATTATTTTCAATTACTAATTTTTTGAAAAAAAAAAT 60
Qy 61 GTGTAAAGTTAAATATAAAAATACATAAGTCAATATTTATATTTTGATATATTATTAAAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 GTGTAAAGTTAAATATAAAAATACATAAGTCAATATTTATATTTTGATATATTATTAAAA 120
Qy 121 TAATATATTTACTAAAATTTAATACAAAATTATATATATACAAACATATTTAAAAATTTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 TAATATATTTACTAAAATTTAATACAAAATTATATATATACAAACATATTTAAAAATTTG 180
Qy 181 TTTATATTTTAAATAAACAACATATATGCATGTTTATTAAATATTTAATTATTATTAATG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 TTTATATTTTAAATAAACAACATATATGCATGTTTATTAAATATTTAATTATTATTAATG 240
Qy 241 TTTATAATACTAGTATTGATCTAATATGTTTATTATGATAATATTTTAATAGAATCAATA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 TTTATAATACTAGTATTGATCTAATATGTTTATTATGATAATATTTTAATAGAATCAATA 300
Qy 301 TAATTTGTTGATTAAAAGTTCATAAATAAGTTTAAAAATAAAACATTTATGTTTATTTTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 TAATTTGTTGATTAAAAGTTCATAAATAAGTTTAAAAATAAAACATTTATGTTTATTTTG 360
Qy 361 TTTTATGAAACAAAGCTATAAACAAATGTGAAATTATGTATAAACATTATATTTATATTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 TTTTATGAAACAAAGCTATAAACAAATGTGAAATTATGTATAAACATTATATTTATATTT 420
Qy 421 TATATTGATAATGTTTATAAATTATTAATCTAATATATTTAATATATAGTTTTATAAATA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 TATATTGATAATGTTTATAAATTATTAATCTAATATATTTAATATATAGTTTTATAAATA 480
Qy 481 AAGTATGTATATTTATTTTATTTTACTTTTTTTTGTTGTAAGAAAAAATATGTATGTTTA 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 AAGTATGTATATTTATTTTATTTTACTTTTTTTTGTTGTAAGAAAAAATATGTATGTTTA 540
Qy 541 TTGTTTATTTATATAAGACTTAGGAAAAATTAATTACCTCATATAGATTGTACAGAAAAT 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 TTGTTTATTTATATAAGACTTAGGAAAAATTAATTACCTCATATAGATTGTACAGAAAAT 600
Qy 601 TAAAATTATGTGTCTCTAATATAGAATGTATAATTAAATGTAAATAAATTATTTTGTATT 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 TAAAATTATGTGTCTCTAATATAGAATGTATAATTAAATGTAAATAAATTATTTTGTATT 660
Qy 661 ATATAGTATAATAAAATTATATAATGTATACAAAATTATAAATTTCTCATATCTTTTCTT 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 ATATAGTATAATAAAATTATATAATGTATACAAAATTATAAATTTCTCATATCTTTTCTT 720
Qy 721 CTAATTTTTATTTATTTATTTTTTTAATGTCAATATGTATAAAGTGTTGGACTCCAATAT 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 CTAATTTTTATTTATTTATTTTTTTAATGTCAATATGTATAAAGTGTTGGACTCCAATAT 780
Qy 781 AAACACTTAAAGTTAAACTTAAATTGAGTTTAATTAATTCAAATTTTTTGTCAAGTTGTA 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 781 AAACACTTAAAGTTAAACTTAAATTGAGTTTAATTAATTCAAATTTTTTGTCAAGTTGTA 840
Qy 841 TTTTTTAAGTAGTTTGTTTGTGCTTGTGGCTTGTTATAAAACATGCTATGCCCTAATTTT 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 841 TTTTTTAAGTAGTTTGTTTGTGCTTGTGGCTTGTTATAAAACATGCTATGCCCTAATTTT 900
Qy 901 GTATACATAACACTAAGGTGGCATTTGGTTGAAAGAAATGAAAACATATATAAAAATAAA 960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 901 GTATACATAACACTAAGGTGGCATTTGGTTGAAAGAAATGAAAACATATATAAAAATAAA 960
Qy 961 AATGAAATAAAATAAAATTTAAAATGTATAAAAAAATCATTAGTTTCTTTTAATGTTATA 1020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 961 AATGAAATAAAATAAAATTTAAAATGTATAAAAAAATCATTAGTTTCTTTTAATGTTATA 1020
Qy 1021 TTAACATGATTTTTTATTCTATTTTAAAATAGAATAGTCATTCCACTAAAATAGTGTAAA 1080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1021 TTAACATGATTTTTTATTCTATTTTAAAATAGAATAGTCATTCCACTAAAATAGTGTAAA 1080
Qy 1081 GGACATTCCATTAGAATGATATTCTAATACTTTAAAATGTAACCAAATAAATAAATAAAA 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1081 GGACATTCCATTAGAATGATATTCTAATACTTTAAAATGTAACCAAATAAATAAATAAAA 1140
Qy 1141 TAAAAATAATTTCTTTTTCATTCTATTCCATTTCATTACTCATTTTCATTCCATTCTATT 1200
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1141 TAAAAATAATTTCTTTTTCATTCTATTCCATTTCATTACTCATTTTCATTCCATTCTATT 1200
Qy 1201 TCATTTAACAAAAATGATAAATGAACATTTTTGTTATCTTGTAGTAGTACAAACTACAAA 1260
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1201 TCATTTAACAAAAATGATAAATGAACATTTTTGTTATCTTGTAGTAGTACAAACTACAAA 1260
Qy 1261 TAATAGAAAAATGAAACTTTAATATAAGGAGGAGACAAAGTCTATTATTTACCTTGGTGA 1320
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1261 TAATAGAAAAATGAAACTTTAATATAAGGAGGAGACAAAGTCTATTATTTACCTTGGTGA 1320
Qy 1321 TCAAGTTTTATATACAACTTTGAAATAAGTACTTTCTTTCTCTCTCTTATTAACATAATC 1380
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1321 TCAAGTTTTATATACAACTTTGAAATAAGTACTTTCTTTCTCTCTCTTATTAACATAATC 1380
Qy 1381 CTACTTGAGTTCCCACTTGCATTTACCTTAATATAATACTATGTCAACCATGTGTATAAT 1440
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1381 CTACTTGAGTTCCCACTTGCATTTACCTTAATATAATACTATGTCAACCATGTGTATAAT 1440
Qy 1441 TAGATCTAACATCTACTTCAAATCTAAAGAACTTATTTACCAACTAATCAAAATCAAAAT 1500
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1441 TAGATCTAACATCTACTTCAAATCTAAAGAACTTATTTACCAACTAATCAAAATCAAAAT 150
RESULT 1
US-19-225-122-47
Query Match 100.0%; Score 1500; DB 1; Length 1500;
Best Local Similarity 100.0%;
Matches 1500; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 GTAGTACATAAATTTATAGCTGATGATAAGTCTCATTCTCAGTCGAGGAATATCTACTGT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 GTAGTACATAAATTTATAGCTGATGATAAGTCTCATTCTCAGTCGAGGAATATCTACTGT 60
Qy 61 GTTTTGGATCAACTGAAAAGGGAGCTTAAAAGTTCATCCATAGCTCAATTTTCAACCATG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 GTTTTGGATCAACTGAAAAGGGAGCTTAAAAGTTCATCCATAGCTCAATTTTCAACCATG 120
Qy 121 TATTTTTAGTTTATAATAGTAAAGATTATTCAGTCCCGTATAGAGAGAAAACAAATGACA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 TATTTTTAGTTTATAATAGTAAAGATTATTCAGTCCCGTATAGAGAGAAAACAAATGACA 180
Qy 181 AATCATATGATTCAGCAGCAAAAAATGGACTTTTTACAACTCAAAATAAAACAGAAATAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 AATCATATGATTCAGCAGCAAAAAATGGACTTTTTACAACTCAAAATAAAACAGAAATAT 240
Qy 241 CTTACTATAATATAATACATAGGGATAATGTACGTTGATCGGTTTATGTTAAGTACAGTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 CTTACTATAATATAATACATAGGGATAATGTACGTTGATCGGTTTATGTTAAGTACAGTT 300
Qy 301 CGTCTAATTAAAAAAAAAAAAAAACAGAAACCAATCCAATAAAATTAGTCGAGTTAGTCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 CGTCTAATTAAAAAAAAAAAAAAACAGAAACCAATCCAATAAAATTAGTCGAGTTAGTCA 360
Qy 361 GGTTAATCCGTCCAAATCGATCATTAATATATTTTTTTTACATTCAATTGCTATATTTTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 GGTTAATCCGTCCAAATCGATCATTAATATATTTTTTTTACATTCAATTGCTATATTTTA 420
Qy 421 ATTAGATGTAATAACGAGACAAATAGATATATATTTATAAATTACACTTACAGTTCAATT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 ATTAGATGTAATAACGAGACAAATAGATATATATTTATAAATTACACTTACAGTTCAATT 480
Qy 481 CAACACATAAAAGAAACATATTAATTTTAACTTTATATGTTTTAAAAATTCATATAAAGT 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 CAACACATAAAAGAAACATATTAATTTTAACTTTATATGTTTTAAAAATTCATATAAAGT 540
Qy 541 ATATGATATATATAAATATGTATATACATAAAAACCCTTTTGATTGTGTTGGTTGGATTA 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 ATATGATATATATAAATATGTATATACATAAAAACCCTTTTGATTGTGTTGGTTGGATTA 600
Qy 601 GTAAGAGTTGAATAGTGGGGTTGATATTATTTTCAATCCACCCAATATAAAGATCAGACA 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 GTAAGAGTTGAATAGTGGGGTTGATATTATTTTCAATCCACCCAATATAAAGATCAGACA 660
Qy 661 GATTATATTTTTATCGAATTTTTCGATTTGTATTTTTTGTCAGCTTATCTCAATTTAATT 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 GATTATATTTTTATCGAATTTTTCGATTTGTATTTTTTGTCAGCTTATCTCAATTTAATT 720
Qy 721 TAAGCAAGTTATTCAGATTAGGCGGTTTAAAAAAATGAAATAATAGCATGAAAGTTTTCA 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 TAAGCAAGTTATTCAGATTAGGCGGTTTAAAAAAATGAAATAATAGCATGAAAGTTTTCA 780
Qy 781 GTCATAACATTAAAAAAAAAAATGTCATTTTGTTATGATGAGTGGCTATGAACTGCAGCC 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 781 GTCATAACATTAAAAAAAAAAATGTCATTTTGTTATGATGAGTGGCTATGAACTGCAGCC 840
Qy 841 AGTTTTTGATGGTATTCATATGCAATTTTAATGGCTTTCTTCAGCTTAATGATTTAATTA 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 841 AGTTTTTGATGGTATTCATATGCAATTTTAATGGCTTTCTTCAGCTTAATGATTTAATTA 900
Qy 901 GTAGATAAGAGATTCTTGAATCTAGTACTCATGATGTATTTGATATCCATAGTGACCATT 960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 901 GTAGATAAGAGATTCTTGAATCTAGTACTCATGATGTATTTGATATCCATAGTGACCATT 960
Qy 961 GAAGATTGAAACAGTTTTAACTTGGCTTCTCTCTCATATTGTTCAAGCATTATTTTTGGA 1020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 961 GAAGATTGAAACAGTTTTAACTTGGCTTCTCTCTCATATTGTTCAAGCATTATTTTTGGA 1020
Qy 1021 ACATGACGAAAGTTGTTCAGGAACCCATGTTGTCCACATCCTGAATTTCCAAGCTTAAGA 1080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1021 ACATGACGAAAGTTGTTCAGGAACCCATGTTGTCCACATCCTGAATTTCCAAGCTTAAGA 1080
Qy 1081 GAACAAAAAATCCAAATAGGCATTCATCACTAACTTCACAGACATATGTCACATATCTCT 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1081 GAACAAAAAATCCAAATAGGCATTCATCACTAACTTCACAGACATATGTCACATATCTCT 1140
Qy 1141 ACATTTAATCAAAACTGTTGTATGCTGATGTCTTCAATTCACAAGTGGCAAAAATGTAAG 1200
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1141 ACATTTAATCAAAACTGTTGTATGCTGATGTCTTCAATTCACAAGTGGCAAAAATGTAAG 1200
Qy 1201 GTGGGACCCCAAAAAATTTCCACTAGAGCCAAACTCTAAATAGGGAAAACTCTACACCAC 1260
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1201 GTGGGACCCCAAAAAATTTCCACTAGAGCCAAACTCTAAATAGGGAAAACTCTACACCAC 1260
Qy 1261 CTCGTAGTACCCACCGAAATCCCATACCACTCATCTTTTAGAAAAGAAAAAAACCACACT 1320
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1261 CTCGTAGTACCCACCGAAATCCCATACCACTCATCTTTTAGAAAAGAAAAAAACCACACT 1320
Qy 1321 TGATCTCACAACAAATATAAAGCTGACTTCTTTTGTTGTTTTTAGAAACCATTTCCACCA 1380
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1321 TGATCTCACAACAAATATAAAGCTGACTTCTTTTGTTGTTTTTAGAAACCATTTCCACCA 1380
Qy 1381 TTTTCCTAATAAACTACTTGATACTAAAATAATCAAAATAAGAATAAAGGAAAGGAAAGG 1440
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1381 TTTTCCTAATAAACTACTTGATACTAAAATAATCAAAATAAGAATAAAGGAAAGGAAAGG 1440
Qy 1441 AAATAATACTACAACACTGCAATTATTTTCTTTCTCCTGTTAACCAAGTTTGAGGTTAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1441 AAATAATACTACAACACTGCAATTATTTTCTTTCTCCTGTTAACCAAGTTTGAGGTTAGG 1500
Instant SEQ ID NOs: 46 and 47, have 100% identity to the cis-abienol synthase polypeptides of SEQ ID NOs: 20 and 21 of copending application ‘122 (see Table 1 of both Specifications):
RESULT 1
US-19-225-122-20
Query Match 100.0%; Score 4169; DB 1; Length 792;
Best Local Similarity 100.0%;
Matches 792; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 MVLGLRSKIIPLPDHKLGNIKLGSVTNAICHRPCRVRCSHSTASSMEEAKERIRETFGKI 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 MVLGLRSKIIPLPDHKLGNIKLGSVTNAICHRPCRVRCSHSTASSMEEAKERIRETFGKI 60
Qy 61 ELSPSSYDTAWVAMVPSRYSMNQPCFPQCLDWILENQREDGSWGLNPSHPLLVKDSLSST 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 ELSPSSYDTAWVAMVPSRYSMNQPCFPQCLDWILENQREDGSWGLNPSHPLLVKDSLSST 120
Qy 121 LASLLALRKWRIGDNQVQRGLGFIETHGWAVDNKDQISPLGFEIIFPCMINYAEKLNLDL 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 LASLLALRKWRIGDNQVQRGLGFIETHGWAVDNKDQISPLGFEIIFPCMINYAEKLNLDL 180
Qy 181 PLDPNLVNMMLCERELTIERALKNEFEGNMANVEYFAEGLGELCHWKEMMLRQRHNGSLF 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 PLDPNLVNMMLCERELTIERALKNEFEGNMANVEYFAEGLGELCHWKEMMLRQRHNGSLF 240
Qy 241 DSPATTAAALIYHQYDEKCFGYLNSILKLHDNWVPTICPTKIHSNLFLVDALQNLGVDRY 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 DSPATTAAALIYHQYDEKCFGYLNSILKLHDNWVPTICPTKIHSNLFLVDALQNLGVDRY 300
Qy 301 FKTEVKRVLDEIYRLWLEKNEEIFSDVAHCAMAFRLLRMNNYEVSSEELEGFVDQEHFFT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 FKTEVKRVLDEIYRLWLEKNEEIFSDVAHCAMAFRLLRMNNYEVSSEELEGFVDQEHFFT 360
Qy 361 TSSGKLMNHVAILELHRASQVAIHERKDHILDKISTWTRNFMEQKLLDKHIPDRSKKEME 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 TSSGKLMNHVAILELHRASQVAIHERKDHILDKISTWTRNFMEQKLLDKHIPDRSKKEME 420
Qy 421 FAMRKFYGTFDRVETRRYIESYKMDSFKILKAAYRSSGINNIDLLKFSEHDFNLCQTRHK 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 FAMRKFYGTFDRVETRRYIESYKMDSFKILKAAYRSSGINNIDLLKFSEHDFNLCQTRHK 480
Qy 481 EELQQMKRWFTDCKLEQVGLSQQYLYTSYFIIAAILFEPEYADARLAYAKYAIIITAVDD 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 EELQQMKRWFTDCKLEQVGLSQQYLYTSYFIIAAILFEPEYADARLAYAKYAIIITAVDD 540
Qy 541 FFDCFICKEELQNIIELVERWEGYSTVGFRSERVRIFFLALYKMVEEIAAKAETKQGRCV 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 FFDCFICKEELQNIIELVERWEGYSTVGFRSERVRIFFLALYKMVEEIAAKAETKQGRCV 600
Qy 601 KDHLINLWIDMLKCMLVELDLWKIKSTTPSIEEYLSVACVTIGVPCFVLTSLYLLGPKLS 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 KDHLINLWIDMLKCMLVELDLWKIKSTTPSIEEYLSVACVTIGVPCFVLTSLYLLGPKLS 660
Qy 661 KDVIESSEVSALCNCTAAVARLINDIHSYKREQAESSTNMVSILITQSQGTISEEEAIRQ 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 KDVIESSEVSALCNCTAAVARLINDIHSYKREQAESSTNMVSILITQSQGTISEEEAIRQ 720
Qy 721 IKEMMESKRRELLGMVLQNKESQLPQVCKDLFWTTINAAYSIHTHGDGYRFPEEFKNHIN 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 IKEMMESKRRELLGMVLQNKESQLPQVCKDLFWTTINAAYSIHTHGDGYRFPEEFKNHIN 780
Qy 781 DVIYKPLNQYSP 792
||||||||||||
Db 781 DVIYKPLNQYSP 792
RESULT 1
US-19-225-122-21
Query Match 100.0%; Score 3619; DB 1; Length 687;
Best Local Similarity 100.0%;
Matches 687; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 MILGLRSKIIPLPDHKLGNIKLGSVTNAICHRPCRVRCSHSTASSMEEAKERIRETFGKI 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 MILGLRSKIIPLPDHKLGNIKLGSVTNAICHRPCRVRCSHSTASSMEEAKERIRETFGKI 60
Qy 61 ELSPSSYDTAWVAMVPSRYSMNQPCFPQCLDWILENQREDGSWGLNPSHPLLVKDSLSST 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 ELSPSSYDTAWVAMVPSRYSMNQPCFPQCLDWILENQREDGSWGLNPSHPLLVKDSLSST 120
Qy 121 LASLLALRKWRIGDNQVQRGLGFIETHGWAVDNKDQISPLGFEIIFPCMINYAEKLNLDL 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 LASLLALRKWRIGDNQVQRGLGFIETHGWAVDNKDQISPLGFEIIFPCMINYAEKLNLDL 180
Qy 181 PLDPNLVNMMLCERELTIERALKNEFEGNMANVEYFAEGLGELCHWKEMMLRQRHNGSLF 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 PLDPNLVNMMLCERELTIERALKNEFEGNMANVEYFAEGLGELCHWKEMMLRQRHNGSLF 240
Qy 241 DSPATTAAALIYHQYDEKCFGYLNSILKLHDNWVPTICPTKIHSNLFLVDALQNLGVDRY 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 DSPATTAAALIYHQYDEKCFGYLNSILKLHDNWVPTICPTKIHSNLFLVDALQNLGVDRY 300
Qy 301 FKTEVKRVLDEIYRLWLEKNEEIFSDVAHCAMAFRLLRMNNYEVSSEELEGFVDQEHFFT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 FKTEVKRVLDEIYRLWLEKNEEIFSDVAHCAMAFRLLRMNNYEVSSEELEGFVDQEHFFT 360
Qy 361 TSSGKLMNHVAILELHRASQVAIHERKDHILDKISTWTRNFMEQKLLDKHIPDRSKKEME 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 TSSGKLMNHVAILELHRASQVAIHERKDHILDKISTWTRNFMEQKLLDKHIPDRSKKEME 420
Qy 421 FAMRKFYGTFDRVETRRYIESYKMDSFKILKAAYRWEGYSTVGFRSERVRIFFLALYKMV 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 FAMRKFYGTFDRVETRRYIESYKMDSFKILKAAYRWEGYSTVGFRSERVRIFFLALYKMV 480
Qy 481 EEIAAKAETKQGRCVKDHLINLWIDMLKCMLVELDLWKIKSTTPSIEEYLSVACVTIGVP 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 EEIAAKAETKQGRCVKDHLINLWIDMLKCMLVELDLWKIKSTTPSIEEYLSVACVTIGVP 540
Qy 541 CFVLTSLYLLGPKLSKDVIESSEVSALCNCTAAVARLINDIHSYKREQAESSTNMVSILI 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 CFVLTSLYLLGPKLSKDVIESSEVSALCNCTAAVARLINDIHSYKREQAESSTNMVSILI 600
Qy 601 TQSQGTISEEEAIRQIKEMMESKRRELLGMVLQNKESQLPQVCKDLFWTTINAAYSIHTH 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 TQSQGTISEEEAIRQIKEMMESKRRELLGMVLQNKESQLPQVCKDLFWTTINAAYSIHTH 660
Qy 661 GDGYRFPEEFKNHINDVIYKPLNQYSP 687
|||||||||||||||||||||||||||
Db 661 GDGYRFPEEFKNHINDVIYKPLNQYSP 687
Instant SEQ ID NOs: 4 and 5, have 100% identity to the cis-abienol synthase encoding polynucleotides of SEQ ID NOs: 29 and 30 of copending application ‘122 (see Table 1 of both Specifications):
RESULT 1
US-19-225-122-29
Query Match 100.0%; Score 2379; DB 1; Length 2379;
Best Local Similarity 100.0%;
Matches 2379; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 ATGGTACTTGGACTGAGAAGCAAAATCATACCACTTCCTGATCATAAGTTGGGAAATATC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 ATGGTACTTGGACTGAGAAGCAAAATCATACCACTTCCTGATCATAAGTTGGGAAATATC 60
Qy 61 AAATTAGGTTCAGTAACCAATGCAATTTGCCACAGACCATGTAGAGTAAGATGCAGCCAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 AAATTAGGTTCAGTAACCAATGCAATTTGCCACAGACCATGTAGAGTAAGATGCAGCCAC 120
Qy 121 AGTACTGCTTCATCAATGGAAGAGGCAAAGGAGAGAATAAGGGAAACATTTGGAAAAATA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 AGTACTGCTTCATCAATGGAAGAGGCAAAGGAGAGAATAAGGGAAACATTTGGAAAAATA 180
Qy 181 GAGCTATCTCCTTCTTCCTATGACACAGCATGGGTAGCTATGGTCCCTTCAAGATATTCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 GAGCTATCTCCTTCTTCCTATGACACAGCATGGGTAGCTATGGTCCCTTCAAGATATTCT 240
Qy 241 ATGAACCAACCATGTTTTCCTCAGTGCTTAGATTGGATTCTTGAAAATCAAAGAGAAGAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 ATGAACCAACCATGTTTTCCTCAGTGCTTAGATTGGATTCTTGAAAATCAAAGAGAAGAT 300
Qy 301 GGATCTTGGGGCCTAAATCCTAGCCATCCATTGCTTGTAAAAGACTCCCTTTCTTCCACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 GGATCTTGGGGCCTAAATCCTAGCCATCCATTGCTTGTAAAAGACTCCCTTTCTTCCACT 360
Qy 361 CTAGCATCTTTGCTTGCCCTTCGCAAATGGAGAATTGGAGATAACCAAGTCCAAAGAGGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 CTAGCATCTTTGCTTGCCCTTCGCAAATGGAGAATTGGAGATAACCAAGTCCAAAGAGGC 420
Qy 421 CTTGGCTTTATTGAAACGCATGGTTGGGCAGTCGATAACAAGGATCAGATTTCACCTTTA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 CTTGGCTTTATTGAAACGCATGGTTGGGCAGTCGATAACAAGGATCAGATTTCACCTTTA 480
Qy 481 GGATTTGAAATTATATTTCCCTGCATGATCAACTATGCAGAGAAACTTAATTTGGATCTA 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 GGATTTGAAATTATATTTCCCTGCATGATCAACTATGCAGAGAAACTTAATTTGGATCTA 540
Qy 541 CCTTTGGATCCTAACCTTGTAAATATGATGCTCTGCGAACGTGAATTAACAATTGAAAGA 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 CCTTTGGATCCTAACCTTGTAAATATGATGCTCTGCGAACGTGAATTAACAATTGAAAGA 600
Qy 601 GCCTTAAAGAATGAATTCGAGGGGAATATGGCAAATGTAGAATATTTTGCTGAAGGGCTC 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 GCCTTAAAGAATGAATTCGAGGGGAATATGGCAAATGTAGAATATTTTGCTGAAGGGCTC 660
Qy 661 GGTGAATTATGTCATTGGAAAGAGATGATGCTTCGTCAGAGACACAACGGGTCGCTCTTT 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 GGTGAATTATGTCATTGGAAAGAGATGATGCTTCGTCAGAGACACAACGGGTCGCTCTTT 720
Qy 721 GATTCACCAGCCACTACTGCAGCTGCCTTGATTTACCATCAGTACGATGAGAAATGCTTT 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 GATTCACCAGCCACTACTGCAGCTGCCTTGATTTACCATCAGTACGATGAGAAATGCTTT 780
Qy 781 GGGTACTTGAACTCAATCTTGAAACTGCACGATAATTGGGTCCCCACTATTTGCCCTACA 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 781 GGGTACTTGAACTCAATCTTGAAACTGCACGATAATTGGGTCCCCACTATTTGCCCTACA 840
Qy 841 AAGATACATTCAAATCTCTTCTTAGTTGATGCCCTTCAAAATCTTGGAGTAGATCGGTAT 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 841 AAGATACATTCAAATCTCTTCTTAGTTGATGCCCTTCAAAATCTTGGAGTAGATCGGTAT 900
Qy 901 TTTAAAACAGAAGTCAAAAGAGTACTAGATGAAATATACAGGCTTTGGCTAGAAAAGAAT 960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 901 TTTAAAACAGAAGTCAAAAGAGTACTAGATGAAATATACAGGCTTTGGCTAGAAAAGAAT 960
Qy 961 GAAGAAATTTTTTCAGACGTTGCTCATTGTGCCATGGCGTTTCGACTTTTACGGATGAAT 1020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 961 GAAGAAATTTTTTCAGACGTTGCTCATTGTGCCATGGCGTTTCGACTTTTACGGATGAAT 1020
Qy 1021 AACTATGAAGTTTCCTCAGAAGAACTTGAAGGATTTGTCGACCAAGAACATTTCTTTACA 1080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1021 AACTATGAAGTTTCCTCAGAAGAACTTGAAGGATTTGTCGACCAAGAACATTTCTTTACA 1080
Qy 1081 ACATCAAGTGGGAAACTTATGAATCACGTTGCAATTCTCGAACTTCACCGAGCTTCACAG 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1081 ACATCAAGTGGGAAACTTATGAATCACGTTGCAATTCTCGAACTTCACCGAGCTTCACAG 1140
Qy 1141 GTGGCTATTCATGAAAGGAAAGATCACATTTTAGATAAAATAAGTACTTGGACAAGGAAT 1200
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1141 GTGGCTATTCATGAAAGGAAAGATCACATTTTAGATAAAATAAGTACTTGGACAAGGAAT 1200
Qy 1201 TTTATGGAGCAAAAACTCTTGGACAAGCACATCCCTGATAGGTCAAAGAAGGAGATGGAA 1260
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1201 TTTATGGAGCAAAAACTCTTGGACAAGCACATCCCTGATAGGTCAAAGAAGGAGATGGAA 1260
Qy 1261 TTTGCTATGAGGAAATTTTATGGCACATTTGATCGAGTGGAAACTAGACGTTACATCGAG 1320
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1261 TTTGCTATGAGGAAATTTTATGGCACATTTGATCGAGTGGAAACTAGACGTTACATCGAG 1320
Qy 1321 TCATACAAAATGGACAGTTTTAAGATCTTAAAAGCGGCTTACAGGTCTTCCGGTATTAAC 1380
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1321 TCATACAAAATGGACAGTTTTAAGATCTTAAAAGCGGCTTACAGGTCTTCCGGTATTAAC 1380
Qy 1381 AACATAGACTTGCTAAAGTTCTCAGAACACGATTTTAACTTGTGCCAAACCCGACACAAA 1440
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1381 AACATAGACTTGCTAAAGTTCTCAGAACACGATTTTAACTTGTGCCAAACCCGACACAAA 1440
Qy 1441 GAAGAACTTCAACAGATGAAAAGGTGGTTCACAGATTGCAAACTCGAACAAGTAGGATTA 1500
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1441 GAAGAACTTCAACAGATGAAAAGGTGGTTCACAGATTGCAAACTCGAACAAGTAGGATTA 1500
Qy 1501 TCACAACAGTACTTATACACTAGTTACTTCATAATTGCTGCCATACTCTTTGAACCTGAA 1560
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1501 TCACAACAGTACTTATACACTAGTTACTTCATAATTGCTGCCATACTCTTTGAACCTGAA 1560
Qy 1561 TATGCTGATGCTCGTCTAGCATATGCAAAGTACGCCATAATAATAACAGCGGTGGATGAT 1620
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1561 TATGCTGATGCTCGTCTAGCATATGCAAAGTACGCCATAATAATAACAGCGGTGGATGAT 1620
Qy 1621 TTCTTCGATTGTTTTATTTGCAAAGAAGAACTGCAAAACATCATCGAATTAGTAGAGAGA 1680
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1621 TTCTTCGATTGTTTTATTTGCAAAGAAGAACTGCAAAACATCATCGAATTAGTAGAGAGA 1680
Qy 1681 TGGGAGGGATACTCAACCGTCGGATTCCGTTCAGAGAGGGTTAGAATTTTCTTTTTGGCA 1740
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1681 TGGGAGGGATACTCAACCGTCGGATTCCGTTCAGAGAGGGTTAGAATTTTCTTTTTGGCA 1740
Qy 1741 CTTTACAAAATGGTAGAGGAAATTGCGGCAAAGGCGGAAACTAAGCAAGGTCGATGTGTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1741 CTTTACAAAATGGTAGAGGAAATTGCGGCAAAGGCGGAAACTAAGCAAGGTCGATGTGTC 1800
Qy 1801 AAAGATCACCTTATTAACTTGTGGATTGATATGTTGAAGTGTATGCTGGTGGAATTGGAC 1860
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1801 AAAGATCACCTTATTAACTTGTGGATTGATATGTTGAAGTGTATGCTGGTGGAATTGGAC 1860
Qy 1861 CTTTGGAAAATTAAATCAACTACCCCAAGCATAGAGGAGTACTTGTCTGTTGCATGTGTA 1920
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1861 CTTTGGAAAATTAAATCAACTACCCCAAGCATAGAGGAGTACTTGTCTGTTGCATGTGTA 1920
Qy 1921 ACTATTGGTGTTCCATGTTTTGTTCTCACATCACTATATCTTCTTGGACCAAAACTGTCC 1980
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1921 ACTATTGGTGTTCCATGTTTTGTTCTCACATCACTATATCTTCTTGGACCAAAACTGTCC 1980
Qy 1981 AAGGATGTCATAGAAAGTTCTGAGGTCAGTGCCTTATGCAATTGTACAGCTGCTGTGGCC 2040
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1981 AAGGATGTCATAGAAAGTTCTGAGGTCAGTGCCTTATGCAATTGTACAGCTGCTGTGGCC 2040
Qy 2041 CGATTGATTAATGATATACACAGTTACAAGAGAGAACAAGCAGAAAGTTCAACAAATATG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2041 CGATTGATTAATGATATACACAGTTACAAGAGAGAACAAGCAGAAAGTTCAACAAATATG 2100
Qy 2101 GTATCAATATTAATAACACAAAGTCAGGGAACTATCTCTGAAGAAGAGGCTATAAGACAG 2160
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2101 GTATCAATATTAATAACACAAAGTCAGGGAACTATCTCTGAAGAAGAGGCTATAAGACAG 2160
Qy 2161 ATAAAGGAAATGATGGAAAGTAAGAGAAGAGAGTTGCTAGGGATGGTTCTACAAAATAAA 2220
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2161 ATAAAGGAAATGATGGAAAGTAAGAGAAGAGAGTTGCTAGGGATGGTTCTACAAAATAAA 2220
Qy 2221 GAAAGCCAATTGCCACAAGTGTGCAAGGATCTTTTTTGGACGACAATCAACGCAGCTTAT 2280
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2221 GAAAGCCAATTGCCACAAGTGTGCAAGGATCTTTTTTGGACGACAATCAACGCAGCTTAT 2280
Qy 2281 TCTATACATACACATGGCGATGGGTATCGCTTCCCAGAGGAATTCAAGAACCATATCAAC 2340
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2281 TCTATACATACACATGGCGATGGGTATCGCTTCCCAGAGGAATTCAAGAACCATATCAAC 2340
Qy 2341 GATGTAATTTACAAACCACTCAATCAATATTCCCCATAA 2379
|||||||||||||||||||||||||||||||||||||||
Db 2341 GATGTAATTTACAAACCACTCAATCAATATTCCCCATAA 2379
RESULT 1
US-19-225-122-30
Query Match 100.0%; Score 2064; DB 1; Length 2064;
Best Local Similarity 100.0%;
Matches 2064; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 ATGATACTTGGACTGAGAAGCAAAATCATACCACTTCCTGATCATAAGTTGGGAAATATC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 ATGATACTTGGACTGAGAAGCAAAATCATACCACTTCCTGATCATAAGTTGGGAAATATC 60
Qy 61 AAATTAGGTTCAGTAACCAATGCAATTTGCCACAGACCATGTAGAGTAAGATGCAGCCAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 AAATTAGGTTCAGTAACCAATGCAATTTGCCACAGACCATGTAGAGTAAGATGCAGCCAC 120
Qy 121 AGTACTGCTTCATCAATGGAAGAGGCAAAGGAGAGAATAAGGGAAACATTTGGAAAAATA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 AGTACTGCTTCATCAATGGAAGAGGCAAAGGAGAGAATAAGGGAAACATTTGGAAAAATA 180
Qy 181 GAGCTATCTCCTTCTTCCTATGACACAGCATGGGTAGCTATGGTCCCTTCAAGATATTCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 GAGCTATCTCCTTCTTCCTATGACACAGCATGGGTAGCTATGGTCCCTTCAAGATATTCT 240
Qy 241 ATGAACCAACCATGTTTTCCTCAGTGCTTAGATTGGATTCTTGAAAATCAAAGAGAAGAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 ATGAACCAACCATGTTTTCCTCAGTGCTTAGATTGGATTCTTGAAAATCAAAGAGAAGAT 300
Qy 301 GGATCTTGGGGCCTAAATCCTAGCCATCCATTGCTTGTAAAAGACTCCCTTTCTTCCACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 GGATCTTGGGGCCTAAATCCTAGCCATCCATTGCTTGTAAAAGACTCCCTTTCTTCCACT 360
Qy 361 CTAGCATCTTTGCTTGCCCTTCGCAAATGGAGAATTGGAGATAACCAAGTCCAAAGAGGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 CTAGCATCTTTGCTTGCCCTTCGCAAATGGAGAATTGGAGATAACCAAGTCCAAAGAGGC 420
Qy 421 CTTGGCTTTATTGAAACGCATGGTTGGGCAGTCGATAACAAGGATCAGATTTCACCTTTA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 CTTGGCTTTATTGAAACGCATGGTTGGGCAGTCGATAACAAGGATCAGATTTCACCTTTA 480
Qy 481 GGATTTGAAATTATATTTCCCTGCATGATCAACTATGCAGAGAAACTTAATTTGGATCTA 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 GGATTTGAAATTATATTTCCCTGCATGATCAACTATGCAGAGAAACTTAATTTGGATCTA 540
Qy 541 CCTTTGGATCCTAACCTTGTAAATATGATGCTCTGCGAACGTGAATTAACAATTGAAAGA 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 CCTTTGGATCCTAACCTTGTAAATATGATGCTCTGCGAACGTGAATTAACAATTGAAAGA 600
Qy 601 GCCTTAAAGAATGAATTCGAGGGGAATATGGCAAATGTAGAATATTTTGCTGAAGGGCTC 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 GCCTTAAAGAATGAATTCGAGGGGAATATGGCAAATGTAGAATATTTTGCTGAAGGGCTC 660
Qy 661 GGTGAATTATGTCATTGGAAAGAGATGATGCTTCGTCAGAGACACAACGGGTCGCTCTTT 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 GGTGAATTATGTCATTGGAAAGAGATGATGCTTCGTCAGAGACACAACGGGTCGCTCTTT 720
Qy 721 GATTCACCAGCCACTACTGCAGCTGCCTTGATTTACCATCAGTACGATGAGAAATGCTTT 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 GATTCACCAGCCACTACTGCAGCTGCCTTGATTTACCATCAGTACGATGAGAAATGCTTT 780
Qy 781 GGGTACTTGAACTCAATCTTGAAACTGCACGATAATTGGGTCCCCACTATTTGCCCTACA 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 781 GGGTACTTGAACTCAATCTTGAAACTGCACGATAATTGGGTCCCCACTATTTGCCCTACA 840
Qy 841 AAGATACATTCAAATCTCTTCTTAGTTGATGCCCTTCAAAATCTTGGAGTAGATCGGTAT 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 841 AAGATACATTCAAATCTCTTCTTAGTTGATGCCCTTCAAAATCTTGGAGTAGATCGGTAT 900
Qy 901 TTTAAAACAGAAGTCAAAAGAGTACTAGATGAAATATACAGGCTTTGGCTAGAAAAGAAT 960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 901 TTTAAAACAGAAGTCAAAAGAGTACTAGATGAAATATACAGGCTTTGGCTAGAAAAGAAT 960
Qy 961 GAAGAAATTTTTTCAGACGTTGCTCATTGTGCCATGGCGTTTCGACTTTTACGGATGAAT 1020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 961 GAAGAAATTTTTTCAGACGTTGCTCATTGTGCCATGGCGTTTCGACTTTTACGGATGAAT 1020
Qy 1021 AACTATGAAGTTTCCTCAGAAGAACTTGAAGGATTTGTCGACCAAGAACATTTCTTTACA 1080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1021 AACTATGAAGTTTCCTCAGAAGAACTTGAAGGATTTGTCGACCAAGAACATTTCTTTACA 1080
Qy 1081 ACATCAAGTGGGAAACTTATGAATCACGTTGCAATTCTCGAACTTCACCGAGCTTCACAG 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1081 ACATCAAGTGGGAAACTTATGAATCACGTTGCAATTCTCGAACTTCACCGAGCTTCACAG 1140
Qy 1141 GTGGCTATTCATGAAAGGAAAGATCACATTTTAGATAAAATAAGTACTTGGACAAGGAAT 1200
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1141 GTGGCTATTCATGAAAGGAAAGATCACATTTTAGATAAAATAAGTACTTGGACAAGGAAT 1200
Qy 1201 TTTATGGAGCAAAAACTCTTGGACAAGCACATCCCTGATAGGTCAAAGAAGGAGATGGAA 1260
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1201 TTTATGGAGCAAAAACTCTTGGACAAGCACATCCCTGATAGGTCAAAGAAGGAGATGGAA 1260
Qy 1261 TTTGCTATGAGGAAATTTTATGGCACATTTGATCGAGTGGAAACTAGACGTTACATCGAG 1320
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1261 TTTGCTATGAGGAAATTTTATGGCACATTTGATCGAGTGGAAACTAGACGTTACATCGAG 1320
Qy 1321 TCATACAAAATGGACAGTTTTAAGATCTTAAAAGCGGCTTACAGATGGGAGGGATACTCA 1380
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1321 TCATACAAAATGGACAGTTTTAAGATCTTAAAAGCGGCTTACAGATGGGAGGGATACTCA 1380
Qy 1381 ACCGTCGGATTCCGTTCAGAGAGGGTTAGAATTTTCTTTTTGGCACTTTACAAAATGGTA 1440
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1381 ACCGTCGGATTCCGTTCAGAGAGGGTTAGAATTTTCTTTTTGGCACTTTACAAAATGGTA 1440
Qy 1441 GAGGAAATTGCGGCAAAGGCGGAAACTAAGCAAGGTCGATGTGTCAAAGATCACCTTATT 1500
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1441 GAGGAAATTGCGGCAAAGGCGGAAACTAAGCAAGGTCGATGTGTCAAAGATCACCTTATT 1500
Qy 1501 AACTTGTGGATTGATATGTTGAAGTGTATGCTGGTGGAATTGGACCTTTGGAAAATTAAA 1560
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1501 AACTTGTGGATTGATATGTTGAAGTGTATGCTGGTGGAATTGGACCTTTGGAAAATTAAA 1560
Qy 1561 TCAACTACCCCAAGCATAGAGGAGTACTTGTCTGTTGCATGTGTAACTATTGGTGTTCCA 1620
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1561 TCAACTACCCCAAGCATAGAGGAGTACTTGTCTGTTGCATGTGTAACTATTGGTGTTCCA 1620
Qy 1621 TGTTTTGTTCTCACATCACTATATCTTCTTGGACCAAAACTGTCCAAGGATGTCATAGAA 1680
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1621 TGTTTTGTTCTCACATCACTATATCTTCTTGGACCAAAACTGTCCAAGGATGTCATAGAA 1680
Qy 1681 AGTTCTGAGGTCAGTGCCTTATGCAATTGTACAGCTGCTGTGGCCCGATTGATTAATGAT 1740
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1681 AGTTCTGAGGTCAGTGCCTTATGCAATTGTACAGCTGCTGTGGCCCGATTGATTAATGAT 1740
Qy 1741 ATACACAGTTACAAGAGAGAACAAGCAGAAAGTTCAACAAATATGGTATCAATATTAATA 1800
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1741 ATACACAGTTACAAGAGAGAACAAGCAGAAAGTTCAACAAATATGGTATCAATATTAATA 1800
Qy 1801 ACACAAAGTCAGGGAACTATCTCTGAAGAAGAGGCTATAAGACAGATAAAGGAAATGATG 1860
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1801 ACACAAAGTCAGGGAACTATCTCTGAAGAAGAGGCTATAAGACAGATAAAGGAAATGATG 1860
Qy 1861 GAAAGTAAGAGAAGAGAGTTGCTAGGGATGGTTCTACAAAATAAAGAAAGCCAATTGCCA 1920
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1861 GAAAGTAAGAGAAGAGAGTTGCTAGGGATGGTTCTACAAAATAAAGAAAGCCAATTGCCA 1920
Qy 1921 CAAGTGTGCAAGGATCTTTTTTGGACGACAATCAACGCAGCTTATTCTATACATACACAT 1980
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1921 CAAGTGTGCAAGGATCTTTTTTGGACGACAATCAACGCAGCTTATTCTATACATACACAT 1980
Qy 1981 GGCGATGGGTATCGCTTCCCAGAGGAATTCAAGAACCATATCAACGATGTAATTTACAAA 2040
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1981 GGCGATGGGTATCGCTTCCCAGAGGAATTCAAGAACCATATCAACGATGTAATTTACAAA 2040
Qy 2041 CCACTCAATCAATATTCCCCATAA 2064
||||||||||||||||||||||||
Db 2041 CCACTCAATCAATATTCCCCATAA 2064
The instant claims and the claims of ‘122 differ in that the claims of ‘122 do not recite all the limitations of instant claim 1 as a single embodiment. However, it would be obvious to do so given the teachings of Rontein et al.
Rontein et al teaches SEQ ID NO: 24, an NtABS (i.e. cis-abienol synthase from tobacco) having 100% sequence identity to instant SEQ ID NO: 46.
Query Match 100.0%; Score 4169; Length 792;
Best Local Similarity 100.0%;
Matches 792; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 MVLGLRSKIIPLPDHKLGNIKLGSVTNAICHRPCRVRCSHSTASSMEEAKERIRETFGKI 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 MVLGLRSKIIPLPDHKLGNIKLGSVTNAICHRPCRVRCSHSTASSMEEAKERIRETFGKI 60
Qy 61 ELSPSSYDTAWVAMVPSRYSMNQPCFPQCLDWILENQREDGSWGLNPSHPLLVKDSLSST 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 ELSPSSYDTAWVAMVPSRYSMNQPCFPQCLDWILENQREDGSWGLNPSHPLLVKDSLSST 120
Qy 121 LASLLALRKWRIGDNQVQRGLGFIETHGWAVDNKDQISPLGFEIIFPCMINYAEKLNLDL 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 LASLLALRKWRIGDNQVQRGLGFIETHGWAVDNKDQISPLGFEIIFPCMINYAEKLNLDL 180
Qy 181 PLDPNLVNMMLCERELTIERALKNEFEGNMANVEYFAEGLGELCHWKEMMLRQRHNGSLF 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 PLDPNLVNMMLCERELTIERALKNEFEGNMANVEYFAEGLGELCHWKEMMLRQRHNGSLF 240
Qy 241 DSPATTAAALIYHQYDEKCFGYLNSILKLHDNWVPTICPTKIHSNLFLVDALQNLGVDRY 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 DSPATTAAALIYHQYDEKCFGYLNSILKLHDNWVPTICPTKIHSNLFLVDALQNLGVDRY 300
Qy 301 FKTEVKRVLDEIYRLWLEKNEEIFSDVAHCAMAFRLLRMNNYEVSSEELEGFVDQEHFFT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 FKTEVKRVLDEIYRLWLEKNEEIFSDVAHCAMAFRLLRMNNYEVSSEELEGFVDQEHFFT 360
Qy 361 TSSGKLMNHVAILELHRASQVAIHERKDHILDKISTWTRNFMEQKLLDKHIPDRSKKEME 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 TSSGKLMNHVAILELHRASQVAIHERKDHILDKISTWTRNFMEQKLLDKHIPDRSKKEME 420
Qy 421 FAMRKFYGTFDRVETRRYIESYKMDSFKILKAAYRSSGINNIDLLKFSEHDFNLCQTRHK 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 FAMRKFYGTFDRVETRRYIESYKMDSFKILKAAYRSSGINNIDLLKFSEHDFNLCQTRHK 480
Qy 481 EELQQMKRWFTDCKLEQVGLSQQYLYTSYFIIAAILFEPEYADARLAYAKYAIIITAVDD 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 EELQQMKRWFTDCKLEQVGLSQQYLYTSYFIIAAILFEPEYADARLAYAKYAIIITAVDD 540
Qy 541 FFDCFICKEELQNIIELVERWEGYSTVGFRSERVRIFFLALYKMVEEIAAKAETKQGRCV 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 FFDCFICKEELQNIIELVERWEGYSTVGFRSERVRIFFLALYKMVEEIAAKAETKQGRCV 600
Qy 601 KDHLINLWIDMLKCMLVELDLWKIKSTTPSIEEYLSVACVTIGVPCFVLTSLYLLGPKLS 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 KDHLINLWIDMLKCMLVELDLWKIKSTTPSIEEYLSVACVTIGVPCFVLTSLYLLGPKLS 660
Qy 661 KDVIESSEVSALCNCTAAVARLINDIHSYKREQAESSTNMVSILITQSQGTISEEEAIRQ 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 KDVIESSEVSALCNCTAAVARLINDIHSYKREQAESSTNMVSILITQSQGTISEEEAIRQ 720
Qy 721 IKEMMESKRRELLGMVLQNKESQLPQVCKDLFWTTINAAYSIHTHGDGYRFPEEFKNHIN 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 IKEMMESKRRELLGMVLQNKESQLPQVCKDLFWTTINAAYSIHTHGDGYRFPEEFKNHIN 780
Qy 781 DVIYKPLNQYSP 792
||||||||||||
Db 781 DVIYKPLNQYSP 792
Rontein et al teaches the gene encoding said polypeptide, SEQ ID NO: 23, which has 100% identity to instant SEQ ID NO: 4.
Query Match 100.0%; Score 2379; Length 2379;
Best Local Similarity 100.0%;
Matches 2379; Conservative 0; Mismatches 0; Indels 0; Gaps 0;
Qy 1 ATGGTACTTGGACTGAGAAGCAAAATCATACCACTTCCTGATCATAAGTTGGGAAATATC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 ATGGTACTTGGACTGAGAAGCAAAATCATACCACTTCCTGATCATAAGTTGGGAAATATC 60
Qy 61 AAATTAGGTTCAGTAACCAATGCAATTTGCCACAGACCATGTAGAGTAAGATGCAGCCAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 AAATTAGGTTCAGTAACCAATGCAATTTGCCACAGACCATGTAGAGTAAGATGCAGCCAC 120
Qy 121 AGTACTGCTTCATCAATGGAAGAGGCAAAGGAGAGAATAAGGGAAACATTTGGAAAAATA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 AGTACTGCTTCATCAATGGAAGAGGCAAAGGAGAGAATAAGGGAAACATTTGGAAAAATA 180
Qy 181 GAGCTATCTCCTTCTTCCTATGACACAGCATGGGTAGCTATGGTCCCTTCAAGATATTCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 GAGCTATCTCCTTCTTCCTATGACACAGCATGGGTAGCTATGGTCCCTTCAAGATATTCT 240
Qy 241 ATGAACCAACCATGTTTTCCTCAGTGCTTAGATTGGATTCTTGAAAATCAAAGAGAAGAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 ATGAACCAACCATGTTTTCCTCAGTGCTTAGATTGGATTCTTGAAAATCAAAGAGAAGAT 300
Qy 301 GGATCTTGGGGCCTAAATCCTAGCCATCCATTGCTTGTAAAAGACTCCCTTTCTTCCACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 GGATCTTGGGGCCTAAATCCTAGCCATCCATTGCTTGTAAAAGACTCCCTTTCTTCCACT 360
Qy 361 CTAGCATCTTTGCTTGCCCTTCGCAAATGGAGAATTGGAGATAACCAAGTCCAAAGAGGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 CTAGCATCTTTGCTTGCCCTTCGCAAATGGAGAATTGGAGATAACCAAGTCCAAAGAGGC 420
Qy 421 CTTGGCTTTATTGAAACGCATGGTTGGGCAGTCGATAACAAGGATCAGATTTCACCTTTA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 CTTGGCTTTATTGAAACGCATGGTTGGGCAGTCGATAACAAGGATCAGATTTCACCTTTA 480
Qy 481 GGATTTGAAATTATATTTCCCTGCATGATCAACTATGCAGAGAAACTTAATTTGGATCTA 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 GGATTTGAAATTATATTTCCCTGCATGATCAACTATGCAGAGAAACTTAATTTGGATCTA 540
Qy 541 CCTTTGGATCCTAACCTTGTAAATATGATGCTCTGCGAACGTGAATTAACAATTGAAAGA 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 CCTTTGGATCCTAACCTTGTAAATATGATGCTCTGCGAACGTGAATTAACAATTGAAAGA 600
Qy 601 GCCTTAAAGAATGAATTCGAGGGGAATATGGCAAATGTAGAATATTTTGCTGAAGGGCTC 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 GCCTTAAAGAATGAATTCGAGGGGAATATGGCAAATGTAGAATATTTTGCTGAAGGGCTC 660
Qy 661 GGTGAATTATGTCATTGGAAAGAGATGATGCTTCGTCAGAGACACAACGGGTCGCTCTTT 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 GGTGAATTATGTCATTGGAAAGAGATGATGCTTCGTCAGAGACACAACGGGTCGCTCTTT 720
Qy 721 GATTCACCAGCCACTACTGCAGCTGCCTTGATTTACCATCAGTACGATGAGAAATGCTTT 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 GATTCACCAGCCACTACTGCAGCTGCCTTGATTTACCATCAGTACGATGAGAAATGCTTT 780
Qy 781 GGGTACTTGAACTCAATCTTGAAACTGCACGATAATTGGGTCCCCACTATTTGCCCTACA 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 781 GGGTACTTGAACTCAATCTTGAAACTGCACGATAATTGGGTCCCCACTATTTGCCCTACA 840
Qy 841 AAGATACATTCAAATCTCTTCTTAGTTGATGCCCTTCAAAATCTTGGAGTAGATCGGTAT 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 841 AAGATACATTCAAATCTCTTCTTAGTTGATGCCCTTCAAAATCTTGGAGTAGATCGGTAT 900
Qy 901 TTTAAAACAGAAGTCAAAAGAGTACTAGATGAAATATACAGGCTTTGGCTAGAAAAGAAT 960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 901 TTTAAAACAGAAGTCAAAAGAGTACTAGATGAAATATACAGGCTTTGGCTAGAAAAGAAT 960
Qy 961 GAAGAAATTTTTTCAGACGTTGCTCATTGTGCCATGGCGTTTCGACTTTTACGGATGAAT 1020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 961 GAAGAAATTTTTTCAGACGTTGCTCATTGTGCCATGGCGTTTCGACTTTTACGGATGAAT 1020
Qy 1021 AACTATGAAGTTTCCTCAGAAGAACTTGAAGGATTTGTCGACCAAGAACATTTCTTTACA 1080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1021 AACTATGAAGTTTCCTCAGAAGAACTTGAAGGATTTGTCGACCAAGAACATTTCTTTACA 1080
Qy 1081 ACATCAAGTGGGAAACTTATGAATCACGTTGCAATTCTCGAACTTCACCGAGCTTCACAG 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1081 ACATCAAGTGGGAAACTTATGAATCACGTTGCAATTCTCGAACTTCACCGAGCTTCACAG 1140
Qy 1141 GTGGCTATTCATGAAAGGAAAGATCACATTTTAGATAAAATAAGTACTTGGACAAGGAAT 1200
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1141 GTGGCTATTCATGAAAGGAAAGATCACATTTTAGATAAAATAAGTACTTGGACAAGGAAT 1200
Qy 1201 TTTATGGAGCAAAAACTCTTGGACAAGCACATCCCTGATAGGTCAAAGAAGGAGATGGAA 1260
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1201 TTTATGGAGCAAAAACTCTTGGACAAGCACATCCCTGATAGGTCAAAGAAGGAGATGGAA 1260
Qy 1261 TTTGCTATGAGGAAATTTTATGGCACATTTGATCGAGTGGAAACTAGACGTTACATCGAG 1320
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1261 TTTGCTATGAGGAAATTTTATGGCACATTTGATCGAGTGGAAACTAGACGTTACATCGAG 1320
Qy 1321 TCATACAAAATGGACAGTTTTAAGATCTTAAAAGCGGCTTACAGGTCTTCCGGTATTAAC 1380
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1321 TCATACAAAATGGACAGTTTTAAGATCTTAAAAGCGGCTTACAGGTCTTCCGGTATTAAC 1380
Qy 1381 AACATAGACTTGCTAAAGTTCTCAGAACACGATTTTAACTTGTGCCAAACCCGACACAAA 1440
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1381 AACATAGACTTGCTAAAGTTCTCAGAACACGATTTTAACTTGTGCCAAACCCGACACAAA 1440
Qy 1441 GAAGAACTTCAACAGATGAAAAGGTGGTTCACAGATTGCAAACTCGAACAAGTAGGATTA 1500
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1441 GAAGAACTTCAACAGATGAAAAGGTGGTTCACAGATTGCAAACTCGAACAAGTAGGATTA 1500
Qy 1501 TCACAACAGTACTTATACACTAGTTACTTCATAATTGCTGCCATACTCTTTGAACCTGAA 1560
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1501 TCACAACAGTACTTATACACTAGTTACTTCATAATTGCTGCCATACTCTTTGAACCTGAA 1560
Qy 1561 TATGCTGATGCTCGTCTAGCATATGCAAAGTACGCCATAATAATAACAGCGGTGGATGAT 1620
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1561 TATGCTGATGCTCGTCTAGCATATGCAAAGTACGCCATAATAATAACAGCGGTGGATGAT 1620
Qy 1621 TTCTTCGATTGTTTTATTTGCAAAGAAGAACTGCAAAACATCATCGAATTAGTAGAGAGA 1680
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1621 TTCTTCGATTGTTTTATTTGCAAAGAAGAACTGCAAAACATCATCGAATTAGTAGAGAGA 1680
Qy 1681 TGGGAGGGATACTCAACCGTCGGATTCCGTTCAGAGAGGGTTAGAATTTTCTTTTTGGCA 1740
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1681 TGGGAGGGATACTCAACCGTCGGATTCCGTTCAGAGAGGGTTAGAATTTTCTTTTTGGCA 1740
Qy 1741 CTTTACAAAATGGTAGAGGAAATTGCGGCAAAGGCGGAAACTAAGCAAGGTCGATGTGTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1741 CTTTACAAAATGGTAGAGGAAATTGCGGCAAAGGCGGAAACTAAGCAAGGTCGATGTGTC 1800
Qy 1801 AAAGATCACCTTATTAACTTGTGGATTGATATGTTGAAGTGTATGCTGGTGGAATTGGAC 1860
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1801 AAAGATCACCTTATTAACTTGTGGATTGATATGTTGAAGTGTATGCTGGTGGAATTGGAC 1860
Qy 1861 CTTTGGAAAATTAAATCAACTACCCCAAGCATAGAGGAGTACTTGTCTGTTGCATGTGTA 1920
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1861 CTTTGGAAAATTAAATCAACTACCCCAAGCATAGAGGAGTACTTGTCTGTTGCATGTGTA 1920
Qy 1921 ACTATTGGTGTTCCATGTTTTGTTCTCACATCACTATATCTTCTTGGACCAAAACTGTCC 1980
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1921 ACTATTGGTGTTCCATGTTTTGTTCTCACATCACTATATCTTCTTGGACCAAAACTGTCC 1980
Qy 1981 AAGGATGTCATAGAAAGTTCTGAGGTCAGTGCCTTATGCAATTGTACAGCTGCTGTGGCC 2040
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1981 AAGGATGTCATAGAAAGTTCTGAGGTCAGTGCCTTATGCAATTGTACAGCTGCTGTGGCC 2040
Qy 2041 CGATTGATTAATGATATACACAGTTACAAGAGAGAACAAGCAGAAAGTTCAACAAATATG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2041 CGATTGATTAATGATATACACAGTTACAAGAGAGAACAAGCAGAAAGTTCAACAAATATG 2100
Qy 2101 GTATCAATATTAATAACACAAAGTCAGGGAACTATCTCTGAAGAAGAGGCTATAAGACAG 2160
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2101 GTATCAATATTAATAACACAAAGTCAGGGAACTATCTCTGAAGAAGAGGCTATAAGACAG 2160
Qy 2161 ATAAAGGAAATGATGGAAAGTAAGAGAAGAGAGTTGCTAGGGATGGTTCTACAAAATAAA 2220
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2161 ATAAAGGAAATGATGGAAAGTAAGAGAAGAGAGTTGCTAGGGATGGTTCTACAAAATAAA 2220
Qy 2221 GAAAGCCAATTGCCACAAGTGTGCAAGGATCTTTTTTGGACGACAATCAACGCAGCTTAT 2280
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2221 GAAAGCCAATTGCCACAAGTGTGCAAGGATCTTTTTTGGACGACAATCAACGCAGCTTAT 2280
Qy 2281 TCTATACATACACATGGCGATGGGTATCGCTTCCCAGAGGAATTCAAGAACCATATCAAC 2340
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2281 TCTATACATACACATGGCGATGGGTATCGCTTCCCAGAGGAATTCAAGAACCATATCAAC 2340
Qy 2341 GATGTAATTTACAAACCACTCAATCAATATTCCCCATAA 2379
|||||||||||||||||||||||||||||||||||||||
Db 2341 GATGTAATTTACAAACCACTCAATCAATATTCCCCATAA 2379
Rontein et al teaches a tobacco plant comprising an expression cassette comprising a gene encoding a cis-abienol synthase polypeptide comprising a sequence with 100% identity to instant SEQ ID NO: 46 and encoded by a polynucleotide with 100% sequence identity to instant SEQ ID NO: 4; operably linked to a heterologous promoter (claims 1-5, 9-10; Figure 3).
Rontein teaches that is preferable to use a tissue specific promoter, such as a tobacco trichome specific promoter (pages 17-18; Figure 3).
Therefore, it would be obvious to combine the claims of ‘122 into a single embodiment as required by the claims of the instant application.
This is a provisional nonstatutory double patenting rejection.
Conclusion
No claims are allowed.
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/ALEKSANDAR RADOSAVLJEVIC/ Examiner, Art Unit 1662 /BRENT T PAGE/Primary Examiner, Art Unit 1663
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