Prosecution Insights
Last updated: April 19, 2026
Application No. 17/688,116

Reagents And Methods For The Analysis of Linked Nucleic Acids

Non-Final OA §DP
Filed
Mar 07, 2022
Examiner
ZHANG, KAIJIANG
Art Unit
1684
Tech Center
1600 — Biotechnology & Organic Chemistry
Assignee
Cs Genetics Limited
OA Round
1 (Non-Final)
76%
Grant Probability
Favorable
1-2
OA Rounds
2y 11m
To Grant
99%
With Interview

Examiner Intelligence

Grants 76% — above average
76%
Career Allow Rate
518 granted / 678 resolved
+16.4% vs TC avg
Strong +36% interview lift
Without
With
+35.5%
Interview Lift
resolved cases with interview
Typical timeline
2y 11m
Avg Prosecution
28 currently pending
Career history
706
Total Applications
across all art units

Statute-Specific Performance

§101
6.3%
-33.7% vs TC avg
§103
26.5%
-13.5% vs TC avg
§102
24.5%
-15.5% vs TC avg
§112
26.6%
-13.4% vs TC avg
Black line = Tech Center average estimate • Based on career data from 678 resolved cases

Office Action

§DP
DETAILED ACTION Notice of Pre-AIA or AIA Status 1. The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . Double Patenting 2. The nonstatutory double patenting rejection is based on a judicially created doctrine grounded in public policy (a policy reflected in the statute) so as to prevent the unjustified or improper timewise extension of the “right to exclude” granted by a patent and to prevent possible harassment by multiple assignees. A nonstatutory double patenting rejection is appropriate where the conflicting claims are not identical, but at least one examined application claim is not patentably distinct from the reference claim(s) because the examined application claim is either anticipated by, or would have been obvious over, the reference claim(s). See, e.g., In re Berg, 140 F.3d 1428, 46 USPQ2d 1226 (Fed. Cir. 1998); In re Goodman, 11 F.3d 1046, 29 USPQ2d 2010 (Fed. Cir. 1993); In re Longi, 759 F.2d 887, 225 USPQ 645 (Fed. Cir. 1985); In re Van Ornum, 686 F.2d 937, 214 USPQ 761 (CCPA 1982); In re Vogel, 422 F.2d 438, 164 USPQ 619 (CCPA 1970); In re Thorington, 418 F.2d 528, 163 USPQ 644 (CCPA 1969). A timely filed terminal disclaimer in compliance with 37 CFR 1.321(c) or 1.321(d) may be used to overcome an actual or provisional rejection based on nonstatutory double patenting provided the reference application or patent either is shown to be commonly owned with the examined application, or claims an invention made as a result of activities undertaken within the scope of a joint research agreement. See MPEP § 717.02 for applications subject to examination under the first inventor to file provisions of the AIA as explained in MPEP § 2159. See MPEP § 2146 et seq. for applications not subject to examination under the first inventor to file provisions of the AIA . A terminal disclaimer must be signed in compliance with 37 CFR 1.321(b). The filing of a terminal disclaimer by itself is not a complete reply to a nonstatutory double patenting (NSDP) rejection. A complete reply requires that the terminal disclaimer be accompanied by a reply requesting reconsideration of the prior Office action. Even where the NSDP rejection is provisional the reply must be complete. See MPEP § 804, subsection I.B.1. For a reply to a non-final Office action, see 37 CFR 1.111(a). For a reply to final Office action, see 37 CFR 1.113(c). A request for reconsideration while not provided for in 37 CFR 1.113(c) may be filed after final for consideration. See MPEP §§ 706.07(e) and 714.13. The USPTO Internet website contains terminal disclaimer forms which may be used. Please visit www.uspto.gov/patent/patents-forms. The actual filing date of the application in which the form is filed determines what form (e.g., PTO/SB/25, PTO/SB/26, PTO/AIA /25, or PTO/AIA /26) should be used. A web-based eTerminal Disclaimer may be filled out completely online using web-screens. An eTerminal Disclaimer that meets all requirements is auto-processed and approved immediately upon submission. For more information about eTerminal Disclaimers, refer to www.uspto.gov/patents/apply/applying-online/eterminal-disclaimer. 3. Claims 22-38 are rejected on the ground of nonstatutory double patenting as being unpatentable over claims 1-17 of U.S. Patent No. 10,640,811 in view of Booth et al. (US 2014/0178881 A1). Claims 1-17 of U.S. Patent No. 10,640,811 teach all the steps and elements as recited in instant claims 22-38, except the feature of measuring 5-hydroxy-methylcytosine (i.e., the method disclosed in claims 1-17 of U.S. Patent No. 10,640,811 involves measuring 5-methylcytosine instead). However, Booth et al. teach that, in metazoa, 5-methylcytosine (5mC) can be oxidized to 5-hydroxy-methylcytosine (5hmc) which also constitutes an epigenetic mark (see paragraph [0002]). Booth et al. further teach measuring both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) (see the whole document, e.g., Abstract; paragraphs [0002]-[0011]). It would have been prima facie obvious to one of ordinary skill in the art before the effective filling date of the claimed invention to measure 5-hydroxy-methylcytosine (5hmc) (or measure both 5-methylcytosine (5mC) and 5-hydroxy-methylcytosine (5hmC)), as taught by Booth et al., in the claimed method of U.S. Patent No. 10,640,811 thus arriving at the instantly claimed invention, because: 1) the sequencing process in the claimed method of U.S. Patent No. 10,640,811 is a general or generic sequencing process that can be used for measuring any sequence variants or modifications including 5-hydroxy-methylcytosine (5hmC); 2) Booth et al. taught that, in metazoa, 5-methylcytosine (5mC) can be oxidized to 5-hydroxy-methylcytosine (5hmc) which also constitutes an epigenetic mark (see paragraph [0002]). Given the teachings of the prior art and the level of the ordinary skilled artisan at the effective filling date of the applicant’s claimed invention, it must be considered, absent evidence to the contrary, that said skilled artisan would have had a reasonable expectation of success in practicing the claimed invention. 4. Claims 22-38 are rejected on the ground of nonstatutory double patenting as being unpatentable over claims 1-13 of U.S. Patent No. 10,287,624 in view of Booth et al. (US 2014/0178881 A1). Regarding claims 22 and 25 Claim 1 of U.S. Patent No. 10,287,624 teach all the features of the instantly claimed method, except the use of the sequencing process for measuring 5-hydroxy-methylcytosine. However, Booth et al. teach sequencing can be used to measure 5-hydroxy-methylcytosine (see the whole document, particularly Abstract; paragraphs [0004]-[0011]) which is produced by oxidation of 5-methylcytosine (which is a well-studied epigenetic DNA mark that plays important roles in gene silencing and genome stability) in metazoa and also constitutes an epigenetic mark (see paragraph [0002]). It would have been prima facie obvious to one of ordinary skill in the art before the effective filling date of the claimed invention to use the sequencing process in the method as recited in claim 1 of U.S. Patent No. 10,287,624 for measuring 5-hydroxy-methylcytosine as taught by Booth et al. thus arriving at the instantly claimed invention, because: 1) the sequencing process in the method as recited in claim 1 of U.S. Patent No. 10,287,624 is a general or generic sequencing process that can be used for measuring any sequence variants or modifications including 5-hydroxy-methylcytosine; 2) Booth et al. taught that, in metazoa, 5-methylcytosine can be oxidized to 5-hydroxy-methylcytosine which also constitutes an epigenetic mark (see paragraph [0002]). Given the teachings of the prior art and the level of the ordinary skilled artisan at the effective filling date of the applicant’s claimed invention, it must be considered, absent evidence to the contrary, that said skilled artisan would have had a reasonable expectation of success in practicing the claimed invention. Regarding claims 23-24 According to Booth et al., the step of measuring 5-hydroxy-methylcytosine (i.e., bisulfite sequencing) comprises: an enrichment process to enrich for fragments of genomic DNA containing 5-hydroxy-methylcytosine (paragraphs [0003] and [0095]), and a bisulfite conversion process (Abstract; paragraphs [0004]-[0011]; claims 1-3 and 21). Regarding claims 26-38 Claims 2-13 of U.S. Patent No. 10,287,624 further disclose all the additional features as recited in instant claims 26-38. 5. Claims 22-38 are rejected on the ground of nonstatutory double patenting as being unpatentable over claims 1-21 of U.S. Patent No. 11,268,131 in view of Booth et al. (US 2014/0178881 A1). Regarding claims 22 and 25 Claim 1 of U.S. Patent No. 11,268,131 teach all the features of the instantly claimed method, except the use of the sequencing process for measuring 5-hydroxy-methylcytosine. However, Booth et al. teach sequencing can be used to measure 5-hydroxy-methylcytosine (see the whole document, particularly Abstract; paragraphs [0004]-[0011]) which is produced by oxidation of 5-methylcytosine (which is a well-studied epigenetic DNA mark that plays important roles in gene silencing and genome stability) in metazoa and also constitutes an epigenetic mark (see paragraph [0002]). It would have been prima facie obvious to one of ordinary skill in the art before the effective filling date of the claimed invention to use the sequencing process in the method as recited in claim 1 of U.S. Patent No. 11,268,131 for measuring 5-hydroxy-methylcytosine as taught by Booth et al. thus arriving at the instantly claimed invention, because: 1) the sequencing process in the method as recited in claim 1 of U.S. Patent No. 11,268,131 is a general or generic sequencing process that can be used for measuring any sequence variants or modifications including 5-hydroxy-methylcytosine; 2) Booth et al. taught that, in metazoa, 5-methylcytosine can be oxidized to 5-hydroxy-methylcytosine which also constitutes an epigenetic mark (see paragraph [0002]). Given the teachings of the prior art and the level of the ordinary skilled artisan at the effective filling date of the applicant’s claimed invention, it must be considered, absent evidence to the contrary, that said skilled artisan would have had a reasonable expectation of success in practicing the claimed invention. Regarding claims 23-24 According to Booth et al., the step of measuring 5-hydroxy-methylcytosine (i.e., bisulfite sequencing) comprises: an enrichment process to enrich for fragments of genomic DNA containing 5-hydroxy-methylcytosine (paragraphs [0003] and [0095]), and a bisulfite conversion process (Abstract; paragraphs [0004]-[0011]; claims 1-3 and 21). Regarding claims 26-38 Claims 2-21 of U.S. Patent No. 11,268,131 further teach all the additional features as recited in instant claims 26-38. Conclusion 6. No claim is allowed. Any inquiry concerning this communication or earlier communications from the examiner should be directed to KAIJIANG ZHANG whose telephone number is (571)272-5207. The examiner can normally be reached Monday - Friday, 8:30 am - 5 pm. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Heather Calamita can be reached on 571-272-2876. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /KAIJIANG ZHANG/Primary Examiner, Art Unit 1684
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Prosecution Timeline

Mar 07, 2022
Application Filed
Nov 25, 2025
Non-Final Rejection — §DP (current)

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Study what changed to get past this examiner. Based on 5 most recent grants.

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Prosecution Projections

1-2
Expected OA Rounds
76%
Grant Probability
99%
With Interview (+35.5%)
2y 11m
Median Time to Grant
Low
PTA Risk
Based on 678 resolved cases by this examiner. Grant probability derived from career allow rate.

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