Prosecution Insights
Last updated: April 19, 2026
Application No. 17/802,377

METHODS AND COMPOSITIONS FOR IDENTIFYING TRAITS USING A CELL-FREE SYSTEM

Non-Final OA §101§102
Filed
Aug 25, 2022
Examiner
BOESEN, CHRISTIAN C
Art Unit
1684
Tech Center
1600 — Biotechnology & Organic Chemistry
Assignee
Synvitrobio, Inc.
OA Round
1 (Non-Final)
75%
Grant Probability
Favorable
1-2
OA Rounds
3y 8m
To Grant
97%
With Interview

Examiner Intelligence

Grants 75% — above average
75%
Career Allow Rate
463 granted / 616 resolved
+15.2% vs TC avg
Strong +22% interview lift
Without
With
+21.9%
Interview Lift
resolved cases with interview
Typical timeline
3y 8m
Avg Prosecution
22 currently pending
Career history
638
Total Applications
across all art units

Statute-Specific Performance

§101
8.5%
-31.5% vs TC avg
§103
28.8%
-11.2% vs TC avg
§102
18.1%
-21.9% vs TC avg
§112
25.4%
-14.6% vs TC avg
Black line = Tech Center average estimate • Based on career data from 616 resolved cases

Office Action

§101 §102
Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . DETAILED ACTION This Non-Final Office Action is responsive to the communication received 8/25/2022. Claims 1-14, 17-18, 20 and 22-24 are pending. Claims 1-14, 17-18, 20 and 22-24 are under examination in this Office Action. Claim Rejections - 35 USC § 101 35 U.S.C. 101 reads as follows: Whoever invents or discovers any new and useful process, machine, manufacture, or composition of matter, or any new and useful improvement thereof, may obtain a patent therefor, subject to the conditions and requirements of this title. Claims 1-14, 17-18, 20 and 22-24 are rejected under 35 U.S.C. 101 because the claimed invention is directed to nonstatutory subject matter. The claim(s) does/do not fall within at least one of the four categories of patent eligible subject matter because the claimed invention is directed to a judicial exception, an abstract idea, without significantly more. Claims 2-14, 17-18, 20 and 22-24 depend directly or indirectly from claim 1. The claim 1 limitations directed to mental processes are determining whether the one or more protein of interest exhibits the desired phenotype. The claim(s) does/do not include additional elements that are sufficient to amount to significantly more than the judicial exception because the claim recites additional elements that consist of well understood, routine, conventional activity already engaged in by the scientific community. The claim 1 limitations directed to well understood, routine, conventional activity already engaged in by the scientific community are a method for screening using an in vitro transcription/translation (TXTL) system, comprising: (a) providing a library of genetic candidates to be screened, each genetic candidate encoding one or more protein of interest; (b) expressing each genetic candidate in an in vitro transcription/translation (TXTL) system, thereby producing a plurality of compositions each comprising the one or more protein of interest; and (c) subjecting the plurality of compositions to an assay for assessing a desired phenotype. Labaer et al (11/24/2025) US Patent Application Publication 2005/0260653 A1 (hereinafter known as "Labaer") teaches a method for screening using an in vitro transcription/translation (TXTL) system, comprising: (a) providing a library of genetic candidates to be screened, each genetic candidate encoding one or more protein of interest; (b) expressing each genetic candidate in an in vitro transcription/translation (TXTL) system, thereby producing a plurality of compositions each comprising the one or more protein of interest; and (c) subjecting the plurality of compositions to an assay for assessing a desired phenotype (see [0391] to [0794]). Claim Rejections - 35 USC § 102 The following is a quotation of the appropriate paragraphs of 35 U.S.C. 102 that form the basis for the rejections under this section made in this Office action: A person shall be entitled to a patent unless – (a)(1) the claimed invention was patented, described in a printed publication, or in public use, on sale or otherwise available to the public before the effective filing date of the claimed invention. Claims 1-14, 17-18, 20 and 22-24 are rejected under 35 U.S.C. 102(a)(1) as being anticipated by Labaer et al (11/24/2025) US Patent Application Publication 2005/0260653 A1 (hereinafter known as "Labaer"). With regards to claims 1-14, 17-18, 20 and 22-24, Labaer teaches: a) as in claims 1-14, 17-18, 20 and 22-24, a method for screening using an in vitro transcription/translation (TXTL) system, comprising: (a) providing a library of genetic candidates to be screened, each genetic candidate encoding one or more protein of interest; (b) expressing each genetic candidate in an in vitro transcription/translation (TXTL) system, thereby producing a plurality of compositions each comprising the one or more protein of interest; and (c) subjecting the plurality of compositions to an assay for assessing a desired phenotype, thereby determining whether the one or more protein of interest exhibits the desired phenotype; wherein the library comprises at least 2 genetic candidates; wherein the library comprises a genome of an organism of interest; wherein the library comprises a plurality of homologs; wherein the library comprises a plurality of variants; wherein each genetic candidate is a gene; wherein each gene is engineered to be under the control of the same promoter; wherein each genetic candidate comprises a linear DNA; wherein step (b) comprises substantially simultaneously expressing all genetic candidates; wherein step (c) comprises substantially simultaneously subjecting the plurality of compositions to the assay; wherein the assay comprises an organism, wherein the organism is an insect; wherein the desired phenotype comprises death or growth of the organism; wherein the subjecting step comprises feeding or injecting each composition to the organism; wherein the desired phenotype is insecticide; further comprising comparing the plurality of compositions to one another to identify a final composition having a desired characteristic; wherein the desired characteristic is production amount; wherein the genetic candidates are variants having alternate codons encoding the same protein, and wherein the library is used for codon optimization and/or identification of internal translation sites; wherein the genetic candidates are variants encoding the same protein, and wherein the library is used to discover domain-shuffled variants; wherein the genetic candidates are engineered genes having different genetic elements, and wherein the library is used to identify optimal transcription and/or translation regulatory units; further comprising purifying the one or more proteins of interest and subjecting the purified proteins to the assay (see [0391] to [0794]). Thus, Labaer anticipates the present claims. Conclusion No claim is allowed. Any inquiry concerning this communication or earlier communications from the Examiner should be directed to Christian Boesen whose telephone number is 571-270-1321. The Examiner can normally be reached on Monday-Friday 9:00 AM to 5:00 PM. If attempts to reach the Examiner by telephone are unsuccessful, the Examiner’s supervisor, Heather Calamita can be reached at 571-272-2876. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of an application may be obtained from the Patent Application Information Retrieval (PAIR) system. Status information for published applications may be obtained from either Private PAIR or Public PAIR. Status information for unpublished applications is available through Private PAIR only. For more information about the PAIR system, see http://pair-direct.uspto.gov. Should you have questions on access to the Private PAIR system, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative or access to the automated information system, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice . /CHRISTIAN C BOESEN/Primary Examiner, Art Unit 1684
Read full office action

Prosecution Timeline

Aug 25, 2022
Application Filed
Sep 25, 2025
Non-Final Rejection — §101, §102 (current)

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Study what changed to get past this examiner. Based on 5 most recent grants.

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Prosecution Projections

1-2
Expected OA Rounds
75%
Grant Probability
97%
With Interview (+21.9%)
3y 8m
Median Time to Grant
Low
PTA Risk
Based on 616 resolved cases by this examiner. Grant probability derived from career allow rate.

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