Prosecution Insights
Last updated: April 19, 2026
Application No. 17/881,038

COMPOUNDS AND METHODS FOR FORMING ION CHANNELS IN BIOLOGICAL MEMBRANES

Final Rejection §102§103
Filed
Aug 04, 2022
Examiner
SIMMONS, CHRIS E
Art Unit
1622
Tech Center
1600 — Biotechnology & Organic Chemistry
Assignee
Wisconsin Alumni Research Foundation
OA Round
6 (Final)
34%
Grant Probability
At Risk
7-8
OA Rounds
3y 10m
To Grant
53%
With Interview

Examiner Intelligence

Grants only 34% of cases
34%
Career Allow Rate
229 granted / 665 resolved
-25.6% vs TC avg
Strong +19% interview lift
Without
With
+18.9%
Interview Lift
resolved cases with interview
Typical timeline
3y 10m
Avg Prosecution
45 currently pending
Career history
710
Total Applications
across all art units

Statute-Specific Performance

§101
1.0%
-39.0% vs TC avg
§103
46.2%
+6.2% vs TC avg
§102
14.3%
-25.7% vs TC avg
§112
23.8%
-16.2% vs TC avg
Black line = Tech Center average estimate • Based on career data from 665 resolved cases

Office Action

§102 §103
DETAILED ACTION Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . Status Claims 3, 5, 7, 10-20, 23, 24, 26 and 28 are pending, wherein Claim 28 is newly added. Claims 10-20 are withdrawn from further consideration pursuant to 37 CFR 1.142(b), as being drawn to a nonelected invention. Therefore, Claims 3, 5, 7, 23, 24, 26 and 28 are presented for examination. Election/Restrictions Applicant elected Invention I (a compound) with traverse in the reply filed on 6/26/2023. Priority This application claims priority to U.S. Provisional Patent Application No. 63/275,987, filed November 5, 2021, and U.S. Provisional Patent Application No. 63/229,265, filed August 4, 2021. Information Disclosure Statement No Information Disclosure Statement was filed with Applicant’s remarks. Withdrawn Claim Rejections Claim 7 was rejected under 35 U.S.C. 102(a)(1) as being anticipated by Tashmukhamedova et al. (“Preparation of acylated and alkylated 2,3-benzo-15-crown-5.” Bioorganicheskaya Khimiya (1980), 6(2), 281-4.) Applicant’s amendment and corresponding reply pertaining to the newly added limitation have overcome the 35 U.S.C. rejections above made of record in the previous Office Action, specifically, the addition of the limitation to Claim 7 that requires if m=2, then n=9. Claim Rejections - 35 USC § 103 Rejection Maintained The following is a quotation of 35 U.S.C. 103 which forms the basis for all obviousness rejections set forth in this Office action: A patent for a claimed invention may not be obtained, notwithstanding that the claimed invention is not identically disclosed as set forth in section 102, if the differences between the claimed invention and the prior art are such that the claimed invention as a whole would have been obvious before the effective filing date of the claimed invention to a person having ordinary skill in the art to which the claimed invention pertains. Patentability shall not be negated by the manner in which the invention was made. The factual inquiries for establishing a background for determining obviousness under 35 U.S.C. 103 are summarized as follows: 1. Determining the scope and contents of the prior art. 2. Ascertaining the differences between the prior art and the claims at issue. 3. Resolving the level of ordinary skill in the pertinent art. 4. Considering objective evidence present in the application indicating obviousness or nonobviousness. This application currently names joint inventors. In considering patentability of the claims the examiner presumes that the subject matter of the various claims was commonly owned as of the effective filing date of the claimed invention(s) absent any evidence to the contrary. Applicant is advised of the obligation under 37 CFR 1.56 to point out the inventor and effective filing dates of each claim that was not commonly owned as of the effective filing date of the later invention in order for the examiner to consider the applicability of 35 U.S.C. 102(b)(2)(C) for any potential 35 U.S.C. 102(a)(2) prior art against the later invention. A. Slightly modified to address amendment - Claims 7, 26, and 28 are rejected under 35 U.S.C. 103 as being unpatentable over by Kikukawa et al. (J. Chem. Soc. Perkin Trans. II 1987.) Claimed invention Claim 7 is drawn to a self-assembling compound for the formation of ion channels in biological membranes, the self-assembling compound being one of a monoacylated benzo(crown-ether) (MAkBCE) compound: MAkBCE PNG media_image1.png 146 296 media_image1.png Greyscale , wherein m is 2 or 3 and n is 4 or 9; wherein is m is 2 then n is 9. Claim 26 limits formula (IIB) of Claim 7 wherein m is 3 and n is 9. Claim 28 limits formula (IIB) of Claim 7 wherein m is 3 and n is 4. Prior art Kikukawa teaches the crown ether, Compound 2b PNG media_image2.png 310 842 media_image2.png Greyscale . See p. 136, 1st column. This compound corresponds to formula (IIB) PNG media_image1.png 146 296 media_image1.png Greyscale , wherein m is 3 and n is 6. Kikukawa further teaches that the crown ethers are useful for solvent extraction and complexation of alkali metal cations. See title. However, Compound 2b differs from the claimed compound because n is 6 instead of 9 (as required in Claim 26) or 4 (as required by Claim 28). However, Kikukawa teaches that Compound 2b possessed the highest extraction percentage for potassium ion as compared to the other substituted benzocrown ethers (Table 1 at p. 138). It can further be seen that the substituted benzocrown ethers can maintain a similar extraction percentage capability for potassium ion even when the length of the alkyl chain differs by a large number of repeating -CH2- groups. For example, Table 1 shows that Compounds 2c and 2d, which are structural homologs amongst each other, possess similar extraction percentages of 56.7% and 56.5% for potassium ion even though these compounds differ structurally by 12 repeating -CH2- groups: PNG media_image3.png 170 186 media_image3.png Greyscale Compound 2c: R = n-C4H9O, Compound 2d: R = n-C16H33O. Compound 2b (where n=6 as it corresponds to instant formula IIB) is a homolog of instant compounds of formula IIB (where n = 9 or 4). “Compounds which are position isomers (compounds having the same radicals in physically different positions on the same nucleus) or homologs (compounds differing regularly by the successive addition of the same chemical group, e.g., by -CH2- groups) are generally of sufficiently close structural similarity that there is a presumed expectation that such compounds possess similar properties.” See In re Wilder, 563 F.2d 457, 195 USPQ 426 (CCPA 1977). See MPEP § 2144.09. In the current case, the compound of formula IIB PNG media_image1.png 146 296 media_image1.png Greyscale (where m is 3 and n is 9 or 4) would have been obvious because Compound 2b (where m is 3 but n is 6) is a homolog differing only by 2 or 3 regular successions of the same chemical group, i.e., by -CH2- groups. The POSA would have initially selected Compound 2b for further modification because it possessed the highest extraction percentage for potassium ion as compared to the other substituted benzocrown ethers. Therefore, the POSA would have found it obvious to modify Compound 2b by altering the length of the alkyl chain by changing the number of -CH2- groups therein, including wherein n is 9 or 4. The POSA would have reasonably expected the suggested compound to be useful for potassium ion extraction. Response to arguments Applicant's arguments have been fully considered but have not been found to be persuasive. Applicant argues that compounds 2c and 2d differ in moiety class from compound 2b and thus their performance is not predictive of 2b derivatives. However, consistent with In re Wilder, the rejection relies on homologous relationship involving regular -CH2- repeating groups variation. Like In re Wilder, the rejection recognizes such variation as a basis for presuming similar properties. MPEP § 2144.09 further explains that such regular homologous changes are generally regarded as predictable modifications. Further, Kikukawa discloses compound 2b (corresponding to instant alkyl chain wherein n is 6, instead of 4 or 9) and compounds 2c (R = n-C4H9O) and 2d (R = n-C16H33O). Despite a twelve-carbon difference in substituent chain length between 2c and 2d, the reported potassium ion extraction efficiencies are nearly identical (56.7% and 56.5%). This demonstrates that substantial variation in carbon-length did not produce significant change in extraction performance. A POSA would reasonably infer from this data that a more modest chain-length adjustment in the n-alkyl chain would likewise not materially affect function. Applicant also argues that the differences in extraction percentages from compounds 1a-e to the homolog compounds 2a-e do not possess similar properties. This is not persuasive because the changes addressed in this rejection is not the crown size (15-crown ethers (1a-e) vs 18-crown ethers (2a-e)), it is rather changes in the methylene chain length. Therefore, Applicants arguments distinguishing between ring size variation and side-chain homologation do not overcome the rejection. B. Rejection maintained - Claims 3, 5, and 7 are rejected under 35 U.S.C. 103 as being unpatentable over Luboch et al. (“The synthesis of some aromatic crown ether derivatives and their ion-selective electrode properties.” Tetrahedron (1990); 46(7): 2461-2472.) Claimed invention Claim 3 is drawn to a self-assembling compound for the formation of ion channels in biological membranes, the self-assembling compound being one of a monoacylated benzo(crown-ether) (MAcBCE) compound: MAcBCE PNG media_image4.png 165 297 media_image4.png Greyscale , wherein m is 2 or 3, and n is 4, 6 or 9, wherein if m is 2, then n is 9. Claim 7 is drawn to a self-assembling compound for the formation of ion channels in biological membranes, the self-assembling compound being one of a monoacylated benzo(crown-ether) (MAkBCE) compound: MAkBCE PNG media_image5.png 146 296 media_image5.png Greyscale , wherein m is 2 or 3 and n is 4 or 9 wherein if m is 2, then n is 9. Prior art Luboch reports on the synthesis of aromatic “crown ether” derivatives and their ion-selective properties. See Luboch, title. Luboch teaches benzo-15-crown-5 and benzo-18-crown-6 derivatives. Specific examples include PNG media_image6.png 390 237 media_image6.png Greyscale . See Fig. 1 at p. 2462. Compounds 5 and 6 in the figure read on the following structures: PNG media_image7.png 82 245 media_image7.png Greyscale (compound 5); PNG media_image8.png 86 258 media_image8.png Greyscale (compound 6). Luboch teaches highly lipophilic crown ether compounds including compounds 5 and 6 are ion carriers that exhibit the best selectivity towards potassium with log kK,Na values of approximately -3.3 to -3.5. Luboch teaches a rise in lipophilicity is also advantageous as it causes an increase stability. See Luboch, pp. 2461 and 2468. Claims 3 and 5 are obvious Compound 5 PNG media_image9.png 82 245 media_image9.png Greyscale differs from the instant compounds of formula IB PNG media_image10.png 149 278 media_image10.png Greyscale because, in Compound 5 of the reference, n would be 8, which falls outside the scope of the claims. However, the compounds of Claims 3 and 5 would have been obvious because of the close structural similarities between Compound 5 and compounds of formula IB. Compound 5 (where n=8) is a homolog of instant compounds of formula IB (where n = 4, 6 and 9). “Compound which are position isomers (compounds having the same radicals in physically different positions on the same nucleus) or homologs (compounds differing regularly by the successive addition of the same chemical group, e.g., by -CH2- groups) are generally of sufficiently close structural similarity that there is a presumed expectation that such compounds possess similar properties.” See In re Wilder, 563 F.2d 457, 195 USPQ 426 (CCPA 1977). See MPEP § 2144.09.In the current case, the compound of formula IB PNG media_image11.png 154 288 media_image11.png Greyscale (where m is 2 and n is 9) would have been obvious to a person of ordinary skill in the art (POSA) because it is a homolog of Compound 5 (where m is 2 but n is 8) differing only by regular successions of the same chemical group, i.e., by -CH2- groups. The POSA would have initially selected Compound 5 for further modification because it was one of the compounds Luboch identified as providing the best selectivity for potassium. Therefore, the POSA would have found it obvious to modify Compound 5 by altering the length of the alkyl chain by changing the number of -CH2- groups therein, including wherein n is 9. Further given the teaching that a rise in lipophilicity is also advantageous as it causes an increase stability, the artisan would have found it obvious to further change the carbon chain length of Compounds 5 and 6 to adjust the lipophilicity in order to optimize stability. The POSA would have reasonably expected the suggested compounds to be useful as potassium ion carriers. Claim 7 is obvious Compound 6 PNG media_image12.png 86 258 media_image12.png Greyscale differs from the instant compounds of formula IIB PNG media_image5.png 146 296 media_image5.png Greyscale because, in Compound 6 of the reference, n would be 8, which falls outside the scope of the claims. However, the compounds of Claim 7 would have been obvious because of the close structural similarities between Compound 6 and compounds of formula IIB. Compound 6 (where n=8) is a homolog of instant compounds of formula IIB (where n = 4 or 9). “Compounds which are position isomers (compounds having the same radicals in physically different positions on the same nucleus) or homologs (compounds differing regularly by the successive addition of the same chemical group, e.g., by -CH2- groups) are generally of sufficiently close structural similarity that there is a presumed expectation that such compounds possess similar properties.” See In re Wilder, 563 F.2d 457, 195 USPQ 426 (CCPA 1977). See MPEP § 2144.09.In the current case, the compound of formula IIB PNG media_image5.png 146 296 media_image5.png Greyscale (where m is 2 and n is 9) would have been obvious because it is a homolog of Compound 6 (where m is 2 but n is 8) differing only by regular successions of the same chemical group, i.e., by -CH2- groups. The POSA would have initially selected Compound 6 for further modification because it was one of the compounds Luboch identified as providing the best selectivity for potassium. Therefore, the POSA would have found it obvious to modify Compound 6 by altering the length of the alkyl chain by changing the number of -CH2- groups therein, including wherein n is 9. Further given the teaching that a rise in lipophilicity is also advantageous as it causes an increase stability, the artisan would have found it obvious to further change the carbon chain length of Compound 6 to adjust the lipophilicity in order to optimize stability. The POSA would have reasonably expected the suggested compounds to be useful as potassium ion carriers. Response to arguments Applicant relies on the same arguments above against Kikukawa (which is not used in this rejection) would not suggest changing the length of the alkyl chain. Applicant adds that Kikukawa teaches that compound 1a (R=H) has an extraction percentage of 14.2 while compound 1b (R=C8H17) has an extraction percentage of 11-5; thus, decreasing extraction. However, this line of argument is not persuasive for reasons set forth above. Additionally, Luboch provides its own internal data directly supporting the value of lipophilic side chain (affected by chain length) in optimizing stability: “A rise in lipophilicity is also advantageous as it causes an increase of the membrane stability.” See p. 2468. Accordingly, the POSA would not rely on Kikukawa to contradict or override the direct structure-activity (chain length-lipophilicity-stability) correlation disclosed in Luboch. Applicant also argues that Luboch does not attribute increased stability to chain length, but instead to branching of the substituent chain and therefore, would not motivate a POSA to alter the number of -CH2- units are proposed in the rejection. However, while certain examples in Luboch involve branched substituents, the reference also includes n-alkyl and n-acyl differing in chain length. For example, Fig. 1 at p. 2462 discloses both n-alkyl and n-acyl compounds of varying lengths: PNG media_image6.png 390 237 media_image6.png Greyscale . Compounds 5 and 6 (the main homologs to the instant compounds used in the rejection) are n-alkyl and n-acyl derivatives – not branched – and are still described as “highly lipophilic compounds” that give the best selectivity. See Luboch, pp. 2461 and 2468.Taken together with the related text at pp. 2467-2468, Luboch does not limit lipophilicity to substituent branching as alleged by Applicant. C. Rejection maintained - Claims 23, 24 and 26 are rejected under 35 U.S.C. 103 as being unpatentable over by Luboch et al., as applied to claims 3, 5, and 7 above, in further view of Frensdorff et al. (“Stability Constants of Cyclic Polyether Complexes with Univalent Cations.” Journal of the American Chemical Society (1971); 93(3): 600-606.) Claimed invention Claim 23 further defines the compounds of Claim 3 wherein m = 3 and Claim 24 further defines the compounds of Claim 23 wherein n = 9. Claims 26 further defines the compounds of Claim 7 and wherein m = 3 and wherein n = 9. Prior art As outlined above, Luboch teaches the compounds PNG media_image13.png 120 108 media_image13.png Greyscale (compound 5) and PNG media_image14.png 86 258 media_image14.png Greyscale (compound 6) and suggests their homolog compounds of formula IB and formula IIB, respectively, including wherein n = 9. While Luboch suggests compounds of instant formulae IB and IIB where m = 2 and n = 9, Luboch does not teach the compounds where m = 3, i.e., wherein the crown is PNG media_image15.png 327 48 media_image15.png Greyscale . However, Luboch teaches highly lipophilic crown ether compounds including compounds 5 and 6 are ion carriers that exhibit the best selectivity towards potassium with log kK,Na values of approximately -3.3 to -3.5. Luboch teaches a rise in lipophilicity is also advantageous as it causes an increase stability. See Luboch, pp. 2461 and 2468. Frensdorff reported the stability constants for the 1:1 complexes of 22 cyclic polyethers (12- to 60-membered rings of C-C-O units with various substituents including nitrogen and sulfur) with several cations (Li+, Na+, K+, Cs+, NH4+, Ag+) in water and in methanol have been determined by potentiometry with cation-selective electrodes. Selectivity toward the different cations varies with polyether ring size, the optimum ring size being such that the cation just fits into the hole, i.e., 15-18 for Na+, 18 for K+, and 18-21 for Cs+. See abstract. Frensdorff teaches that the hole size in the 18-Crown-6 polyether ring PNG media_image15.png 327 48 media_image15.png Greyscale corresponds with the size of the potassium ion diameter. See Table III at p. 603. Frensdorff further teaches that the stability constant (k) for 18-Crown-6 (Type 1 = A(CH2CH2OCH2CH2OCH2CH2)2B where A = B = O) was highest with potassium. See Table VII at p. 605. Therefore, one of ordinary skill in the art (POSA) would have found it obvious to alter the size of the “crown” of two of the compounds that provided the best selectivity for potassium according to Luboch such as the compounds PNG media_image13.png 120 108 media_image13.png Greyscale (compound 5) and PNG media_image16.png 120 360 media_image16.png Greyscale (compound 6) to arrive at the compounds PNG media_image17.png 122 364 media_image17.png Greyscale and PNG media_image18.png 122 364 media_image18.png Greyscale because both Luboch and Frensdorff teach the use of crown ethers to form complexes with metal ions including potassium. Because Frensdorff teaches the 18-Crown-6 polyether ring PNG media_image15.png 327 48 media_image15.png Greyscale corresponds with the diameter of potassium and possesses the highest stability constant (k) for potassium, the POSA would have used the 18-Crown-6 polyether ring PNG media_image15.png 327 48 media_image15.png Greyscale in place of the 15-Crown-5 polyether ring PNG media_image13.png 120 108 media_image13.png Greyscale in either compound 5 or compound 6 of Luboch in order to optimize the compounds affinity or compatibility for potassium ion. Further given that a rise in lipophilicity is also advantageous as it causes an increase stability, the artisan would have found it obvious to further change the carbon chain length of Compounds 5 and 6 as outlined above to adjust the lipophilicity in order to optimize stability. Therefore, the claimed invention as a whole would have been prima facie obvious at the time the invention application was filed. Response to arguments Applicant relies on the same arguments above that Luboch does not suggest varying chain length because Luboch attributes lipophilicity and consequently stability to branching side substituent. This is not deemed to be persuasive as indicated above because Compounds 5 and 6 were both described as “highly lipophilic compounds” that give the best selectivity. Conclusion No claims are allowed. Applicant's amendment necessitated the new ground(s) of rejection presented in this Office action. Accordingly, THIS ACTION IS MADE FINAL. See MPEP § 706.07(a). Applicant is reminded of the extension of time policy as set forth in 37 CFR 1.136(a). A shortened statutory period for reply to this final action is set to expire THREE MONTHS from the mailing date of this action. In the event a first reply is filed within TWO MONTHS of the mailing date of this final action and the advisory action is not mailed until after the end of the THREE-MONTH shortened statutory period, then the shortened statutory period will expire on the date the advisory action is mailed, and any nonprovisional extension fee (37 CFR 1.17(a)) pursuant to 37 CFR 1.136(a) will be calculated from the mailing date of the advisory action. In no event, however, will the statutory period for reply expire later than SIX MONTHS from the mailing date of this final action. Any inquiry concerning this communication or earlier communications from the examiner should be directed to CHRIS E SIMMONS whose telephone number is (571)272-9065. The examiner can normally be reached M-F: 9:30-6:00p. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, James H. Alstrum-Acevedo can be reached on (571) 272-5548. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /CHRIS E SIMMONS/Examiner, Art Unit 1629 /JAMES H ALSTRUM-ACEVEDO/Supervisory Patent Examiner, Art Unit 1622
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Prosecution Timeline

Aug 04, 2022
Application Filed
Sep 26, 2023
Non-Final Rejection — §102, §103
Jan 08, 2024
Response Filed
Apr 06, 2024
Final Rejection — §102, §103
Jul 08, 2024
Response after Non-Final Action
Aug 02, 2024
Examiner Interview (Telephonic)
Aug 15, 2024
Response after Non-Final Action
Aug 29, 2024
Request for Continued Examination
Aug 30, 2024
Response after Non-Final Action
Sep 21, 2024
Non-Final Rejection — §102, §103
Dec 16, 2024
Response Filed
Jan 16, 2025
Final Rejection — §102, §103
Apr 24, 2025
Request for Continued Examination
Apr 28, 2025
Response after Non-Final Action
May 30, 2025
Response Filed
Jul 12, 2025
Non-Final Rejection — §102, §103
Oct 10, 2025
Response Filed
Nov 04, 2025
Final Rejection — §102, §103 (current)

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Expected OA Rounds
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Grant Probability
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