DETAILED ACTION Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA. Election/Restrictions Applicant’s election of Species A: 1; Species B: 1; Species C: 2; Species D: 3; Species E: 1; Species F: 1; Species G: 1; and Species H: 4 in the reply filed on 11/14/2025 is acknowledged. Because applicant did not distinctly and specifically point out the supposed errors in the restriction requirement, the election has been treated as an election without traverse (MPEP § 818.01(a)). Drawings The drawings are objected to under 37 CFR 1.83(a). The drawings must show every feature of the invention specified in the claims. Therefore, the wheelbase (claim 3) , mastermix chamber (claim 17) must be shown or the feature(s) canceled from the claim(s). No new matter should be entered. Corrected drawing sheets in compliance with 37 CFR 1.121(d) are required in reply to the Office action to avoid abandonment of the application. Any amended replacement drawing sheet should include all of the figures appearing on the immediate prior version of the sheet, even if only one figure is being amended. The figure or figure number of an amended drawing should not be labeled as “amended.” If a drawing figure is to be canceled, the appropriate figure must be removed from the replacement sheet, and where necessary, the remaining figures must be renumbered and appropriate changes made to the brief description of the several views of the drawings for consistency. Additional replacement sheets may be necessary to show the renumbering of the remaining figures. Each drawing sheet submitted after the filing date of an application must be labeled in the top margin as either “Replacement Sheet” or “New Sheet” pursuant to 37 CFR 1.121(d). If the changes are not accepted by the examiner, the applicant will be notified and informed of any required corrective action in the next Office action. The objection to the drawings will not be held in abeyance. Claim Rejections - 35 USC § 112 The following is a quotation of 35 U.S.C. 112(b): (b) CONCLUSION.—The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the inventor or a joint inventor regards as the invention. The following is a quotation of 35 U.S.C. 112 (pre-AIA), second paragraph: The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the applicant regards as his invention. Claims 1-20 are rejected under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention. Claim s 1 -20 are not clear with respect to what applicant is claiming. The claim s do not clearly set forth the metes and bounds of the patent protection desired. Regarding c laim 1 , the phrases such as “providing”, “pressurizing”, “controlling”, and “flow path” , etc. make the claim unclear whether the limitations following the phrase are part of the claimed invention. It is unclear if the applicant is claiming first and second pneumatic flow paths , a mixing chamber , a buffer chamber , a first valve, a second valve, a third valve, and a fourth valve , sample , a magnet bead , a buffer , and the target analyte , because these limitations are not positive elements of the claim. In addition, it is unclear if the applica nt is trying to claim a device, or method of using a device. Claims 3 , 7, 9, 13 , 15 and 20 are similarly unclear if the applicant is claiming a motor (claim 3), lysis buffer (claim 7), binding buffer (claim 9), washing buffer (claim 13) , elution buffer (claims 15, 20), and mastermix (claim 17) . For the reasons above, claims relating to these elements are unclear. Claim 3 is vague and unclear reciting “ further comprising a wheelbase on which the magnetic unit, the heating unit, and the ultrasonic unit are mounted, and wherein the controller controls a motor connected to the wheelbase to move each of the magnetic unit, the heating unit, and the ultrasonic unit to a position corresponding to the mixing chamber ”. A wheelbase is generally understood as the distance between the front and rear axles of a vehicle. However, the specification is unclear of the device having a wheelbase distance. In addition to the motor (see above) , it is unclear structural connection between a motor to the wheelbase (distance) to move each of the magnetic unit, the heating unit, and the ultrasonic unit to a position corresponding to the mixing chamber. Claim 4 recites the limitation " the other side of the metering chamber " in L2 . There is insufficient antecedent basis for this limitation in the claim. Claim 4 is unclear reciting “[...] the other side of the metering chamber and then is connected to the waste flow path ” because it is unclear what “the other side” is being directed to, and what “ is ” being connected to the waste flow path. Claim Rejections - 35 USC § 102 In the event the determination of the status of the application as subject to AIA 35 U.S.C. 102 and 103 (or as subject to pre-AIA 35 U.S.C. 102 and 103) is incorrect, any correction of the statutory basis (i.e., changing from AIA to pre-AIA) for the rejection will not be considered a new ground of rejection if the prior art relied upon, and the rationale supporting the rejection, would be the same under either status. The following is a quotation of the appropriate paragraphs of 35 U.S.C. 102 that form the basis for the rejections under this section made in this Office action: A person shall be entitled to a patent unless – (a)(1) the claimed invention was patented, described in a printed publication, or in public use, on sale, or otherwise available to the public before the effective filing date of the claimed invention. (a)(2) the claimed invention was described in a patent issued under section 151, or in an application for patent published or deemed published under section 122(b), in which the patent or application, as the case may be, names another inventor and was effectively filed before the effective filing date of the claimed invention. Claim(s) 1-20 is/are rejected under 35 U.S.C. 102a1/a2 as being anticipated by Handique et al. (US 2007/0292941). Regarding claim 1, Handique et al. teach: 1. A target analyte detection device (e.g., microfluidic cartridge 200/ 300, Figs. 10, 14A, 14B, 15A, and 15B , 50A-50J +) , comprising: a pneumatic unit (e.g., gas actuators (e.g., pumps) Pi ¶ 0322 , Actuators ¶ 0292+) capable of providing pressure to a first and a second pneumatic flow paths of the cartridge (see i.e., An actuator can be a component that provides a gas pressure that can move material (e.g., sample material and/or reagent material) between one location in a network e.g., network 201, and another location. For example, referring to FIG. 13, actuator 244 includes a chamber 272 having a mass 273 of thermally expansive material (TEM) therein. When heated, the TEM expands decreasing the free volume within chamber 272 and pressurizing the gas (e.g., air) surrounding mass 273 within chamber 272. Typically, gates such as gates 246 and 242 in network 201 can be actuated with actuator 244. Consequently, the pressurized gas drives liquid in fluid reservoir 279 towards junction 255. ¶ 0292); a magnetic unit capable of providing an electromagnetic field to a mixing chamber (e.g., 220, 216) of the cartridge (see i.e., In other embodiments, the beads are magnetic, and are concentrated at a particular location of the microfluidic cartridge, such as a chamber or a channel, by applying a magnetic field configured to concentrate lines of flux at the location in question. The magnet employed may be an electromagnet, such as controlled by the processor to switch on and off at specified times during sample analysis. ¶ 0426); a heating unit capable of providing heat to the mixing chamber (see i.e., In various embodiments, the at least one heat pump can be a contact heat source selected from a resistive heater, a radiator, a fluidic heat exchanger and a Peltier device. The contact heat source can be configured at the receiving bay to be thermally coupled to a distinct location in a microfluidic cartridge received in the receiving bay, whereby the distinct location can be selectively heated. At least one additional contact heat source can be included, wherein the contact heat sources can be each configured at the receiving bay to be independently thermally coupled to a different distinct location in a microfluidic cartridge received in the receiving bay, whereby the distinct locations can be independently heated. ¶ 0017); a pressurizing unit capable of pressurizing a buffer chamber of the cartridge (see i.e., Another aspect of the microfluidic cartridge includes a liquid reservoir capable of holding a liquid (e.g., a solvent, a buffer, a reagent, or combination thereof). In general, the reservoir can have one or more of the following features, as further described in international application publication no. WO2006/079082. The reservoir can include a wall that can be manipulated (e.g., pressed or depressed) to decrease a volume within the reservoir. For example, the reservoir can include a piercing member (e.g., a needle-like or otherwise pointed or sharp member) that ruptures another portion of the reservoir (e.g., a portion of the wall) to release liquid. The piercing member can be internal to the reservoir such that the piercing member ruptures the wall from an inner surface of the reservoir (e.g., wall) outwards. ¶ 0248-0249); and a controller capable of controlling opening and closing of a first valve, a second valve, a third valve, and a fourth valve (e.g., valves Vi, double valves V'i , gates Gi, mixing gates MGi ¶ 0322, Figs. 14A-15B) of the cartridge and capable of controlling the pneumatic unit, the magnetic unit, the heating unit, and the pressurizing unit (see Figs. 1, 4 and e.g., programmable processor and a computer program product programmed to operate the apparatus ¶ 0009, 0016, 0079-0086, 0205, 0426+), wherein the cartridge including: a sample chamber (e.g., inlet 202 Fig. 10, lysing chamber 302 having sample input/output ports SP1/SP2 Figs. 14-15) into which sample is inserted (¶ 0263, 0368, 0449-0450, Fig. 65); a metering chamber (e.g., channels in Figs. 10, 14-15 such as C8 & ¶ 0263; channels Ci ¶ 0322; channel M ¶ 0419 ) connected to the sample chamber capable of metering a predetermined amount of sample (see i.e., Upon injection of a sample, the cartridge may be placed in the apparatus 800 and further operations; for example, sample preparation, reagent metering/mixing, and PCR amplification/detection may be performed in an automated and hands-free manner ¶ 0408; In some embodiments, a liquid drop handling system can be used to produce liquid-sample injection and motion based on thermally actuated pumps (e.g. thermo-pneumatic pumping) that may be operated electronically without the use of mechanical valves. For example, by heating air trapped inside chambers that can be connected to the main channel, significant air pressure can be generated for thermo-pneumatic pumping. Increasing the temperature of the air can cause the pressure inside the chamber to rise until the pressure can be high enough to split off a drop (meter an aliquot) and move it to the desired location. This technique can be implemented as an on-cartridge actuation mechanism and may use, for example, molded chambers, channels and heaters. Typically, this can avoid mechanical moving parts and can facilitate fabrication. FIG. 46 shows photos of a demonstration showing the mixing of two fluids (“A”—blue and “B”—orange) using the drop-handling system described above. Pressure pumps P1 and P2 can be activated in a precisely controlled manner which can force the liquids to move as alternating rolling discrete drops along channel M, where they can mix, and finally be positioned into chamber C, where the PCR can take place. ¶ 0419); the mixing chamber (e.g., processing chamber/region 220, retention member 216, first processing region B1) connected to the metering chamber capable of receiving a magnet bead (see i.e., In some embodiments, at least some (e.g., all) of the particles can be magnetic. ¶ 0280; Referring also to FIG. 11, processing chamber 220 includes a plurality of particles (e.g., beads, microspheres) 218 [...] ¶ 0266; Processing region B1 can be configured and operated as processing region 220 of cartridge 200. In some embodiments, first processing region B1 includes a retention member (e.g., multiple particles (e.g., microspheres or beads), a porous member, multiple walls) having at least one surface modified with one or more ligands as described for processing region 220. ¶ 0334; Particles 218 (with polynucleotides) can be retained within processing region B1 ¶ 0378); a waste chamber (e.g., waste reservoir/waste chamber 232; Wi ¶ 0333) connected to the metering chamber (see Figs. 10, 15A-15B, ¶ 0333, 0378+ for example); the buffer chamber (e.g., reservoir 279; reagent reservoirs Ri ¶ 0344) connected to the mixing chamber capable of receiving a buffer (see i.e., In various embodiments, the microfluidic cartridge can further include a reservoir containing a wash buffer, wherein the microfluidic network can be configured to contact the polynucleotide-loaded retention member with the wash buffer, for example, the wash buffer can have a pH of at least about 10. ¶ 0041; During operation of cartridge 200, the liquid of reservoir 279 can typically be used as a wash liquid to remove inhibitors from processing region 220 ¶ 0289; In the configuration shown, reagent reservoir R1 typically holds a release liquid (e.g., a hydroxide solution as described above for cartridge 200) for releasing polynucleotides retained within processing region B1. Reagent reservoir R2 typically holds a wash liquid (e.g., a buffer solution as described above for cartridge 200) [...] ¶ 0349; actuating pump P2 and opening gates G6, G4 and GS to move release liquid from reagent reservoir R1 into processing region B1 and into contact with particles 218. ¶ 0380); a detection chamber (e.g., detection regions D1, D2) connected to the mixing chamber capable of detecting the target analyte (see i.e., typically above one or both of detection regions D1, D2, is a window (as in, e.g., FIG. 9) that permits detection of fluorescence from a fluorescent substance in reaction mixture when a detector is situated above the window. ¶ 0392); the first valve (e.g., V’1/V1’) capable of controlling a fluid flow between the sample chamber and the metering chamber (see Fig. 15B & ¶ 0378 for example); the second valve (e.g., V’6/V6’) capable of controlling a fluid flow between the metering chamber and the mixing chamber (see Fig. 15B & ¶ 0380 for example); the third valve (e.g., V’9/V9’, gates G2, G3 and G9) capable of controlling a fluid flow between the mixing chamber and the waste chamber (see Fig. 15B & ¶ 0379 for example) ; the fourth valve (e.g., V’24/V24’, gates G18, MG20 and G22) capable of controlling a fluid flow between the mixing chamber and the detection chamber (see Figs. 15A-15B & ¶ 0390, 0392 for example); a metering flow path (i.e., downstream channel path of SP2 such as channel C9) connecting the sample chamber and the metering chamber (see Figs. 14-15 for example); a waste flow path (i.e., downstream channel path of channel C18) connecting the metering chamber and the waste chamber (see Fig. 15A for example); a mixing flow path (i.e., upstream channel path of B1 such as channel C8) connecting the metering chamber and the mixing chamber (see Fig. 15A for example); a buffer flow path (i.e., channel path of R1 to B1, such as channel C5/6) connecting the buffer chamber and the mixing chamber (see Fig. 15A for example); a detection flow path (i.e., upstream channel path of D2 such as channel C37) connecting the mixing chamber and the detection chamber (see Fig. 15A for example); a drain flow path (i.e., upstream channel path of W5; flow path of outlet 267, channel 228) connecting the mixing chamber and the waste chamber (see Fig. 15A & i.e., The sample passes through processing region 220, exits via outlet 267, and passes along channel 228 to waste chamber 232. ¶ 0301); the first pneumatic flow path (i.e., flow path of pump P1) communicating with a first pneumatic port and connected to the waste flow path (see Fig. 15A & ¶ 0379 for example); and the second pneumatic flow path (i.e., flow path of pump P2) connected to a second pneumatic port and connected to the mixing chamber (see Fig. 15A & ¶ 0380 for example). With regard to limitations in claims 1-20 (e.g., a pneumatic unit providing pressure to a first and a second pneumatic flow paths of the cartridge; a magnetic unit providing an electromagnetic field to a mixing chamber of the cartridge; a heating unit providing heat to the mixing chamber; a pressurizing unit pressurizing a buffer chamber of the cartridge , etc.), these claim limitations are considered process or intended use limitations, which do not further delineate the structure of the claimed apparatus from that of the prior art. The cited prior art teaches all of the positively recited structure of the claimed apparatus. The recitation of a new intended use, for an old product, does not make a claim to that old product patentable. The Courts have held that a statement of intended use in an apparatus claim fails to distinguish over a prior art apparatus. See In re Sinex , 309 F.2d 488, 492, 135 USPQ 302, 305 (CCPA 1962). The Courts have held that the manner of operating an apparatus does not differentiate an apparatus claim from the prior art, if the prior art apparatus teaches all of the structural limitations of the claim. See Ex Parte Masham , 2 USPQ2d 1647 (BPAI 1987). The Courts have held that apparatus claims must be structurally distinguishable from the prior art in terms of structure, not function. See In re Danley , 120 USPQ 528, 531 (CCPA 1959); and Hewlett-Packard Co. V. Bausch and Lomb , Inc. , 15 USPQ2d 1525, 1528 (Fed. Cir. 1990) (see MPEP §§ 2114 and 2173.05(g)). "Expressions relating the apparatus to contents thereof during an intended operation are of no significance in determining patentability of the apparatus claim." Ex parte Thibault , 164 USPQ 666,667 (Bd. App. 1969). Furthermore, "[i] nclusion of material or article worked upon by a structure being claimed does not impart patentability to the claims." See In re Young , 75 F.2d *>996, 25 USPQ 69 (CCPA 1935) (as restated in In re Otto , 312 F.2d 937, 136 USPQ 458, 459 (CCPA 1963)) (see MPEP § 2115). Regarding claims 2- 20 , Handique et al. teach: 2. The target analyte detection device of claim 1, further comprising an ultrasonic unit capable of providing an ultrasonic wave to the mixing chamber (¶ 0224), and wherein the controller is capable of controlling the ultrasonic unit (see ¶ 0079-0086, 0161+; 0224 for example). 3. The target analyte detection device of claim 2, further comprising a base (e.g., 2000) on which the magnetic unit, the heating unit, and the ultrasonic unit are capable of being mounted (see ¶ 0178+), and wherein the controller is capable of controlling a n automatic mechanical actuator ( ¶ 0439). 4. The target analyte detection device of claim 1, wherein the first pneumatic flow path of the cartridge joins a side of the metering chamber and connected to the waste flow path (see Figs. 15A-15B for example), and wherein the controller is capable of performing a metering chamber filling step (see ¶ 0079-0086, 0161+; 0408, 0419 for example) . 5. The target analyte detection device of claim 4, wherein the controller is capable of performing a metering step (see ¶ 0079-0086, 0161+; 0408, 0419 for example) . 6. The target analyte detection device of claim 5, wherein the controller is capable of performing a mixing chamber transfer step (see ¶ 0079-0086, 0161+; 0331, 0378-0393 for example) . 7. The target analyte detection device of claim 2, wherein in the cartridge, the buffer chamber includes a lysis buffer chamber (see ¶ 0368, one of three LPs in Fig. 14A; and e.g., lysis reservoir 830) capable of receiving a lysis buffer (see i.e., Exemplary Microfluidic Cartridge having a Lysing Chamber [...] Microfluidic cartridge 300 can therefore be a self-contained environment that comprises all reagents and materials necessary to perform steps of workup, cell-lysis, polynucleotide isolation, pre-amplification processing, amplification, and detection of a sample. ¶ 0320-0321+), and the buffer flow path includes a first buffer flow path (i.e., one of three LP flow paths) connecting the lysis buffer chamber and the mixing chamber, and wherein the controller is capable of performing a lysis buffer providing step (see ¶ 0079-0086, 0161+; 0378-0393 for example). 8. The target analyte detection device of claim 7, wherein the controller is capable of performing a lysis mixing step (see ¶ 0079-0086, 0161+; 0378-0393 for example). 9. The target analyte detection device of claim 8, wherein in the cartridge, the buffer chamber further includes a binding buffer chamber (e.g., one of three LPs in Fig. 14A) capable of receiving a binding buffer (¶ 0266, 0368, 0377), and the buffer flow path further includes a second buffer flow path (i.e., one of three LP flow paths) connecting the binding buffer chamber and the mixing chamber, and wherein the controller is capable of performing a binding buffer providing step (see ¶ 0079-0086, 0161+; 0378-0393 for example). 10. The target analyte detection device of claim 9, wherein the controller is capable of performing a first buffer flow path cleaning step (see ¶ 0079-0086, 0161+; 0378-0393 for example) . 11. The target analyte detection device of claim 10, wherein the controller is capable of performing a first incubating step (see Example 12 & Fig. 70A for example). 12. The target analyte detection device of claim 11, wherein the controller is capable of performing a first drain step (see ¶ 0079-0086, 0161+; 0431 for example) . 13. The target analyte detection device of claim 12, wherein in the cartridge, the buffer chamber further includes a washing buffer chamber (e.g., 279) capable of receiving a washing buffer, wherein the buffer flow path further includes a washing buffer flow path connecting the washing buffer chamber and the mixing chamber (e.g., channels 257, 261, and 214 ¶ 0304), and wherein the controller is capable of performing a first washing step (see ¶ 0079-0086, 0161+; 0304 for example). 14. The target analyte detection device of claim 13, wherein the controller is capable of performing a second drain step (see ¶ 0079-0086, 0161+; 0304 for example) . 15. The target analyte detection device of claim 14, wherein in the cartridge, the buffer chamber further includes an elution buffer chamber (e.g., one of three LPs in Fig. 14A) capable of receiving an elution buffer, and the buffer flow path further includes a second buffer flow path connecting the elution buffer chamber and the mixing chamber (see Example 10 for example), and wherein the controller is capable of performing an elution buffer providing step (see ¶ 0079-0086, 0161+; 0454-0457 for example). 16. The target analyte detection device of claim 15, wherein the controller is capable of performing an elution incubating step (see Example 10 for example) . 17. The target analyte detection device of claim 1, wherein the cartridge further includes: a mastermix chamber (e.g., mixing channel C37) provided between the mixing chamber and the detection chamber, connected with the mixing chamber through a mastermix flow path, and receiving the mastermix ; and a fifth valve (e.g., G22) capable of controlling a fluid flow between the mixing chamber and the mastermix chamber (see Figs. 15A-15B & ¶ 0390 for example), wherein the first pneumatic flow path includes a first branched pneumatic flow path connected to the waste flow path and a second branched pneumatic flow path connected to the mastermix chamber (see Figs. 15A-15B for example), and wherein the controller is capable of controlling opening and closing of the fifth valve (see ¶ 0079-0086, 0161+; 0378-0393 for example). 18. The target analyte detection device of claim 8, wherein the controller is capable of providing a positive pressure (see ¶ 0079-0086, 0161+; 0249 for example) . 19. The target analyte detection device of claim 1, wherein the controller is capable of performing a mixing step (see ¶ 0079-0086, 0161+; 0378-0393 for example) . 20. The target analyte detection device of claim 17, wherein in the cartridge, the buffer chamber further includes an elution buffer chamber (e.g., one of three LPs in Fig. 14A) capable of receiving an elution buffer, and the buffer flow path further includes a second buffer flow path connecting the elution buffer chamber and the mixing chamber (see Example 10 for example), and wherein the controller is capable of performing an elution buffer providing step (see ¶ 0079-0086, 0161+; 0454-0457 for example). Conclusion Any inquiry concerning this communication or earlier communications from the examiner should be directed to FILLIN "Examiner name" \* MERGEFORMAT DEAN KWAK whose telephone number is FILLIN "Phone number" \* MERGEFORMAT (571)270-7072 . 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Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /DEAN KWAK/ Primary Examiner, Art Unit 1798 FILLIN "Examiner Stamp" \* MERGEFORMAT DEAN KWAK Primary Examiner Art Unit 1798