Prosecution Insights
Last updated: August 06, 2026
Application No. 18/214,793

THREE-DIMENSIONAL CULTURE DEVICE AND BIOLOGICAL CULTURE INSTRUMENT

Final Rejection §103§112
Filed
Jun 27, 2023
Priority
Feb 02, 2023 — provisional 63/442,776
Examiner
LOPEZLIRA, ASHLEY NICOLE
Art Unit
1799
Tech Center
1700 — Chemical & Materials Engineering
Assignee
Transformative Cell Processing Co. Ltd.
OA Round
2 (Final)
67%
Grant Probability
Favorable
3-4
OA Rounds
8m
Est. Remaining
99%
With Interview

Examiner Intelligence

Grants 67% — above average
67%
Career Allowance Rate
30 granted / 45 resolved
+1.7% vs TC avg
Strong +48% interview lift
Without
With
+48.4%
Interview Lift
resolved cases with interview
Typical timeline
3y 9m
Avg Prosecution
24 currently pending
Career history
72
Total Applications
across all art units

Statute-Specific Performance

§101
0.9%
-39.1% vs TC avg
§103
46.8%
+6.8% vs TC avg
§102
22.4%
-17.6% vs TC avg
§112
27.0%
-13.0% vs TC avg
Black line = Tech Center average estimate • Based on career data from 45 resolved cases

Office Action

§103 §112
DETAILED ACTION Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . Status of the Claims The amendment filed 6/2/2026 has been entered. Claims 1-3 and 5-20 remain pending in the application; claims 13-20 remain withdrawn; and claim 4 has been cancelled. Applicant’s amendments to the claims have overcome each objection and 112(b) rejection previously set forth in the Non-Final Office Action mailed 3/20/2026. Response to Arguments Applicant's arguments filed 6/2/2026 have been fully considered but they are not persuasive. Applicant added limitations to independent claim 1 to include “wherein, in each of the accommodating wells, the filtering bottom structure includes a base and a plurality of upright arms that are connected to the base and that are spaced apart from each other, and any two of the upright arms adjacent to each other have a gap therebetween being smaller than a diameter of any one of the bioparticles”; “each of the insertion pipes has a nozzle abutting against the upright arms of the filtering bottom structure of a corresponding one of the accommodating wells”; “wherein the accommodating wells are enclosed by the cover to maintain air in the culture spaces at a fixed amount”; and “a liquid level of the culture medium in each of the culture spaces to be controlled in a liquid-pressure manner”. These limitations do not distinguish the claimed invention over the prior art, as Zhong et al. (US 2010/0029000 A1) (already of record) in view of Kim et al. (WO 2021/215894 A1) (machine translated) (already of record) teach these limitations, which will be discussed further in the 35 USC § 103 section below. Applicant argues on p. 12 of Remarks that though Kim et al. discloses a micro pattern on the bottom of wells, the micro pattern is not in contact with any tube, and therefore fails to disclose a nozzle abutting against the filtering bottom structure. This is not persuasive, as para. 0034 of Zhong et al. discloses that outlets 50 insert into the wells precisely and consistently, and it is clearly shown in Figs. 7-8 of Zhong et al. that the outlets insert deep enough into the wells such that the outlets are close to the bottom of the wells. Regarding Applicant’s argument that Kim et al. does not disclose a nozzle abutting against the micro pattern, it has been held that the test for obviousness “is what the combined teachings of those references would have suggested to those of ordinary skill in the art… combining the teachings of references does not involve an ability to combine their specific structures (MPEP 2145 III)”. Zhong et al. teaches nozzles/outlets that insert into wells such that they are close to the bottom of the wells and Kim et al. teaches a micro pattern on the bottom of wells; and one of ordinary skill in the art would be motivated to have the nozzle abutting against the micro pattern based on the teachings of both Zhong et al. and Kim et al. Applicant also argues on p. 12 of Remarks that Kim et al. fails to disclose “the accommodating wells are enclosed by the cover to maintain air in the culture spaces at a fixed amount, and the driving mechanism is configured to drive the culture medium in the cover so as to allow the culture medium to flow into or out of each of the culture spaces for enabling a liquid level of the culture medium in each of the culture spaces to be controlled in a liquid-pressure manner”. This is not persuasive, as these limitations are disclosed by Zhong et al., as will be discussed in the 35 USC § 103 section below. Claim Interpretation The following is a quotation of 35 U.S.C. 112(f): (f) Element in Claim for a Combination. – An element in a claim for a combination may be expressed as a means or step for performing a specified function without the recital of structure, material, or acts in support thereof, and such claim shall be construed to cover the corresponding structure, material, or acts described in the specification and equivalents thereof. The following is a quotation of pre-AIA 35 U.S.C. 112, sixth paragraph: An element in a claim for a combination may be expressed as a means or step for performing a specified function without the recital of structure, material, or acts in support thereof, and such claim shall be construed to cover the corresponding structure, material, or acts described in the specification and equivalents thereof. The claims in this application are given their broadest reasonable interpretation using the plain meaning of the claim language in light of the specification as it would be understood by one of ordinary skill in the art. The broadest reasonable interpretation of a claim element (also commonly referred to as a claim limitation) is limited by the description in the specification when 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, is invoked. As explained in MPEP § 2181, subsection I, claim limitations that meet the following three-prong test will be interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph: (A) the claim limitation uses the term “means” or “step” or a term used as a substitute for “means” that is a generic placeholder (also called a nonce term or a non-structural term having no specific structural meaning) for performing the claimed function; (B) the term “means” or “step” or the generic placeholder is modified by functional language, typically, but not always linked by the transition word “for” (e.g., “means for”) or another linking word or phrase, such as “configured to” or “so that”; and (C) the term “means” or “step” or the generic placeholder is not modified by sufficient structure, material, or acts for performing the claimed function. Use of the word “means” (or “step”) in a claim with functional language creates a rebuttable presumption that the claim limitation is to be treated in accordance with 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph. The presumption that the claim limitation is interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, is rebutted when the claim limitation recites sufficient structure, material, or acts to entirely perform the recited function. Absence of the word “means” (or “step”) in a claim creates a rebuttable presumption that the claim limitation is not to be treated in accordance with 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph. The presumption that the claim limitation is not interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, is rebutted when the claim limitation recites function without reciting sufficient structure, material or acts to entirely perform the recited function. Claim limitations in this application that use the word “means” (or “step”) are being interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, except as otherwise indicated in an Office action. Conversely, claim limitations in this application that do not use the word “means” (or “step”) are not being interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, except as otherwise indicated in an Office action. This application includes one or more claim limitations that do not use the word “means,” but are nonetheless being interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, because the claim limitation(s) uses a generic placeholder that is coupled with functional language without reciting sufficient structure to perform the recited function and the generic placeholder is not preceded by a structural modifier. Such claim limitation(s) is/are: "driving mechanism. configured to drive the culture medium" in claim 1. Because this/these claim limitation(s) is/are being interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, it/they is/are being interpreted to cover the corresponding structure described in the specification as performing the claimed function, and equivalents thereof. If applicant does not intend to have this/these limitation(s) interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph, applicant may: (1) amend the claim limitation(s) to avoid it/them being interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph (e.g., by reciting sufficient structure to perform the claimed function); or (2) present a sufficient showing that the claim limitation(s) recite(s) sufficient structure to perform the claimed function so as to avoid it/them being interpreted under 35 U.S.C. 112(f) or pre-AIA 35 U.S.C. 112, sixth paragraph. Claim Rejections - 35 USC § 112 The following is a quotation of 35 U.S.C. 112(d): (d) REFERENCE IN DEPENDENT FORMS.—Subject to subsection (e), a claim in dependent form shall contain a reference to a claim previously set forth and then specify a further limitation of the subject matter claimed. A claim in dependent form shall be construed to incorporate by reference all the limitations of the claim to which it refers. The following is a quotation of pre-AIA 35 U.S.C. 112, fourth paragraph: Subject to the following paragraph [i.e., the fifth paragraph of pre-AIA 35 U.S.C. 112], a claim in dependent form shall contain a reference to a claim previously set forth and then specify a further limitation of the subject matter claimed. A claim in dependent form shall be construed to incorporate by reference all the limitations of the claim to which it refers. Claims 6-8 are rejected under 35 U.S.C. 112(d) or pre-AIA 35 U.S.C. 112, 4th paragraph, as being of improper dependent form for failing to further limit the subject matter of the claim upon which it depends, or for failing to include all the limitations of the claim upon which it depends. Claim 6 does not contain a reference to a claim previously set forth, but instead contains a reference to a cancelled claim (claim 4). For the purposes of examination on the merits, the examiner is interpreting claim 6 as if dependent on claim 1, of which claim 6 specifies a further limitation of the subject matter claimed. Applicant may cancel the claim(s), amend the claim(s) to place the claim(s) in proper dependent form, rewrite the claim(s) in independent form, or present a sufficient showing that the dependent claim(s) complies with the statutory requirements. Dependent claims are rejected for the same reason as the base claim(s) upon which they depend. Claim Rejections - 35 USC § 103 In the event the determination of the status of the application as subject to AIA 35 U.S.C. 102 and 103 (or as subject to pre-AIA 35 U.S.C. 102 and 103) is incorrect, any correction of the statutory basis (i.e., changing from AIA to pre-AIA ) for the rejection will not be considered a new ground of rejection if the prior art relied upon, and the rationale supporting the rejection, would be the same under either status. The following is a quotation of 35 U.S.C. 103 which forms the basis for all obviousness rejections set forth in this Office action: A patent for a claimed invention may not be obtained, notwithstanding that the claimed invention is not identically disclosed as set forth in section 102, if the differences between the claimed invention and the prior art are such that the claimed invention as a whole would have been obvious before the effective filing date of the claimed invention to a person having ordinary skill in the art to which the claimed invention pertains. Patentability shall not be negated by the manner in which the invention was made. Claims 1-3, 6, 8-10, and 12 are rejected under 35 U.S.C. 103 as being unpatentable over Zhong et al. (US 2010/0029000 A1) (already of record) in view of Kim et al. (WO 2021/215894 A1) (machine translated) (already of record). Regarding claim 1, Zhong et al. discloses a culture device for culturing a plurality of bioparticles (abstract “multiwell plate… cell culture”), the 3D culture device comprising: a plurality of accommodating wells (wells 40) each having a surrounding wall, wherein the surrounding wall of each of the accommodating wells defines a culture space therein for accommodating at least one of the bioparticles (wells 40; para. 0038 “culture cells on the bottom of well 40”); a cover (lid 10) including a communication portion (skirt 22) and a plurality of insertion pipes (outlets 50) that are connected to the communication portion, wherein the insertion pipes are in spatial communication with each other through the communication portion, and wherein the insertion pipes are respectively inserted into the culture spaces of the accommodating wells, and each of the insertion pipes has a nozzle corresponding one of the accommodating wells and is in spatial communication with the culture space of the corresponding one of the accommodating wells through the filtering bottom structure (outlets 50, hole/slot/orifice 54); a culture medium that is filled in the culture spaces of the accommodating wells, the communication portion of the cover, and the insertion pipes of the cover, so that the bioparticles respectively located in the culture spaces are immersed in the culture medium (para. 0039 “adding fresh medium to cell culture wells”); and a driving mechanism assembled to the cover (Fig. 7 external pump 100 connected to lid 10), wherein the accommodating wells are enclosed by the cover to maintain air in the culture spaces at a fixed amount (Fig. 1 lid 10 covers wells 40), and the driving mechanism is configured to drive the culture medium in the cover so as to allow the culture medium to flow into or out of each of the culture spaces for enabling a liquid level of the culture medium in each of the culture spaces to be controlled in a liquid-pressure manner (para. 0016 “medium flow is driven by an external pump which can control flow rate”; para. 0037 “fresh medium is driven by an external pump 100 into the compartment 25 of lid 10 through the inlet 60 of the upper section 26 of the lid 10 and then is distributed into wells 40”). Zhong et al. does not teach wells with a filtering bottom structure. However, Kim et al. teaches a micro pattern on the bottom of wells (micro pattern 230) to reduce the contact area between the cell and the culture groove so cells can grow into three-dimensional spheroids having uniform size (para. 0077). It would have been obvious to a person of ordinary skill in the art to use the Kim et al. configuration of a micropattern on the bottom of wells in Zhong et al.’s device with a reasonable expectation that it would reduce the contact area between the cell and the bottom of the well so cells can grow into three-dimensional spheroids having uniform size. This method for improving Zhong et al.’s device was within the ability of one of ordinary skill in the art based on the teachings of Kim et al. Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to combine the teachings of Zhong et al. and Kim et al. to obtain the invention as specified in claim 1. Zhong et al. does not disclose wherein, in each of the accommodating wells, a filtering bottom structure includes a base and a plurality of upright arms that are connected to the base and that are spaced apart from each other, and wherein, in the filtering bottom structure of each of the accommodating wells, any two of the upright arms adjacent to each other have a gap therebetween being smaller than a diameter of any one of the bioparticles. However, Kim et al. teaches a micro pattern on the bottom of wells (micro pattern 230) including a base (culture groove 220) and a plurality of upright arms spaced apart from each other (protrusions 231) wherein upright arms adjacent to each other have a gap therebetween smaller that a diameter of any one of the bioparticles (Figs. 2-8b). Kim et al. teaches that the micro pattern reduces the contact area between the cell and the culture groove so cells can grow into three-dimensional spheroids having uniform size (para. 0077). It would have been obvious to a person of ordinary skill in the art to use the Kim et al. configuration of a micropattern on the bottom of wells in Zhong et al.’s device with a reasonable expectation that it would reduce the contact area between the cell and the bottom of the well so cells can grow into three-dimensional spheroids having uniform size. This method for improving Zhong et al.’s device was within the ability of one of ordinary skill in the art based on the teachings of Kim et al. Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to combine the teachings of Zhong et al. and Kim et al. to obtain the invention as specified in claim 1. Regarding claim 2, Zhong et al. discloses a culture device wherein the culture space of each of the accommodating wells is only in spatial communication with at least one of the insertion pipes arranged therein (Fig. 7 one outlet 50 inserted into each well 40). Regarding claim 3, Zhong et al. discloses a culture device wherein the culture device allows the culture medium to flow among the culture spaces through the cover of the accommodating wells (para. 0037 “fresh medium is driven by an external pump 100 into the compartment 25 of lid 10 through the inlet 60 of the upper section 26 of the lid 10 and then is distributed into wells 40”). Regarding the limitation “culture medium is capable of transmitting biological information generated from the bioparticles respectively located in the culture spaces”, it has been held that a claim containing a recitation with respect to the manner in which a claimed apparatus is intended to be employed does not differentiate the claimed apparatus from a prior art apparatus if the prior art apparatus teaches all the structural limitations of the claim (MPEP § 2114 II). Therefore, the apparatus disclosed by Zhong et al. would be fully capable of achieving every claimed intended use because the prior art apparatus is disclosed to pump culture medium into the wells and release spend medium from the wells (para. 0037) and would be structurally capable of transmitting biological information generated from the bioparticles absent clear evidence otherwise. Zhong et al. does not teach wells with a filtering bottom structure. However, Kim et al. teaches a micro pattern on the bottom of wells (micro pattern 230) to reduce the contact area between the cell and the culture groove so cells can grow into three-dimensional spheroids having uniform size (para. 0077). It would have been obvious to a person of ordinary skill in the art to use the Kim et al. configuration of a micropattern on the bottom of wells in Zhong et al.’s device with a reasonable expectation that it would reduce the contact area between the cell and the bottom of the well so cells can grow into three-dimensional spheroids having uniform size. This method for improving Zhong et al.’s device was within the ability of one of ordinary skill in the art based on the teachings of Kim et al. Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to combine the teachings of Zhong et al. and Kim et al. to obtain the invention as specified in claim 3. Regarding claim 6, Zhong et al. discloses a culture device further comprising: a carrier (plate 20); and a frame that is connected to the carrier to jointly form the accommodating wells, wherein the frame includes the surrounding walls that are spaced apart from each other (sidewalls 14). Zhong et al. does not teach wells with a filtering bottom structure. However, Kim et al. teaches a micro pattern on the bottom of wells (micro pattern 230) to reduce the contact area between the cell and the culture groove so cells can grow into three-dimensional spheroids having uniform size (para. 0077). It would have been obvious to a person of ordinary skill in the art to use the Kim et al. configuration of a micropattern on the bottom of wells in Zhong et al.’s device with a reasonable expectation that it would reduce the contact area between the cell and the bottom of the well so cells can grow into three-dimensional spheroids having uniform size. This method for improving Zhong et al.’s device was within the ability of one of ordinary skill in the art based on the teachings of Kim et al. Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to combine the teachings of Zhong et al. and Kim et al. to obtain the invention as specified in claim 6. Regarding claim 8, Zhong et al. discloses a culture device wherein the frame has a top end surface connected to the surrounding walls (upper section 14A of sidewalls 14), and the communication portion of the cover abuts against the top end surface to enclose the accommodating wells (Fig. 1; para. 0031 “a skirt 22 surrounding the lower section defining a plurality of corners on the lid 10 and extending downwardly”). Regarding claim 9, Zhong et al. discloses a culture device wherein the communication portion has a bottom side (lower section 24) and a top side that is opposite to the bottom side (upper section 26), where the insertion pipes are connected to the bottom side of the communication portion (Fig. 1 outlets 50 connected to lower section 24), wherein the cover includes an external pipe that is connected to the top side and that is in spatial communication with each of the insertion pipes through the communication portion (Fig. 1 inlet 60), and wherein the driving mechanism is assembled to the external pipe (Fig. 7 external pump 100 connected to inlet 60). Regarding claim 10, Zhong et al. discloses a culture device wherein at least one of the insertion pipes has a plurality of filtering holes and is able to be in spatial communication with the corresponding culture space through the filtering holes (hole/slot/orifice 54; Fig. 3). Regarding claim 12, Zhong et al. discloses a culture device wherein the filtering holes of the at least one of the insertion pipes (hole/slot/orifice 54; Fig. 3) are arranged adjacent to the corresponding wells and are immersed in the culture medium (Fig. 7 outlets 50 inserted into wells 40 with culture medium). Zhong et al. does not teach wells with a filtering bottom structure. However, Kim et al. teaches a micro pattern on the bottom of wells (micro pattern 230) to reduce the contact area between the cell and the culture groove so cells can grow into three-dimensional spheroids having uniform size (para. 0077). It would have been obvious to a person of ordinary skill in the art to use the Kim et al. configuration of a micropattern on the bottom of wells in Zhong et al.’s device with a reasonable expectation that it would reduce the contact area between the cell and the bottom of the well so cells can grow into three-dimensional spheroids having uniform size. This method for improving Zhong et al.’s device was within the ability of one of ordinary skill in the art based on the teachings of Kim et al. Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to combine the teachings of Zhong et al. and Kim et al. to obtain the invention as specified in claim 12. Claim 5 is rejected under 35 U.S.C. 103 as being unpatentable over Zhong et al. (US 2010/0029000 A1) (already of record) in view of Kim et al. (WO 2021/215894 A1) (machine translated) (already of record) as applied to claim 1 above, and further in view of Takahashi et al. (US 2013/0323839 A1) (already of record). Regarding claim 5, modified Zhong et al. discloses a culture device comprising a filtering bottom structure comprising upright arms as Kim et al. discloses a micro pattern (230) comprising protrusions (231). Modified Zhong et al. does not disclose wherein a gap between adjacent upright arms/protrusions is less than or equal to 2 µm, or wherein the upright arms/protrusions have an average height being within a range from 5 µm to 15 µm. However, Takahashi et al. teaches a pillar pitch (para. 0061 “distance 107 from the center of the tip of the projection to the center of the tip of the adjacent projection”) ranging from 0.18 to 20 µm (para. 0109) and a pillar height of 1 µm (para. 0061) which control the adhesiveness and migration of cells so that uniformly-sized three-dimensional cell structures are formed (abstract). Regarding the pillar pitch, it has been held that in the case where the claimed ranges overlap or lie inside ranges disclosed by the prior art, a prima facie case of obviousness exists (MPEP § 2144.05). The claimed range of a gap between adjacent upright arms is obvious in view of Takahashi et al. because Takahashi et al. discloses a range of 0.18 to 20 µm, which overlaps the claimed range of less than or equal to 2 µm. It would have been obvious to one of ordinary skill in the art to select the claimed range from the broader prior art range because one of ordinary skill in the art would reasonably expect that a pillar/upright arm gap within the range of 0.18 to 20 µm would help control the adhesiveness and migration of cells so that uniformly-sized three-dimensional cell structures are formed (abstract). Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to select the claimed range from the broader prior art range to obtain the invention as specified in claim 5. Regarding the pillar height, it has been held that a prima facie case of obviousness exists where the claimed ranges or amounts do not overlap with the prior art but are merely close (MPEP 2144.05 I). The claimed range of 5 µm to 15 µm is obvious in view of Takahashi et al. because Takahashi et al. discloses a height of 1 µm which is close to the claimed range. It would have been obvious to one of ordinary skill in the art to select the claimed range from the close prior art range because one of ordinary skill in the art would reasonably expect that a pillar/upright arm height close to 1 µm would help control the adhesiveness and migration of cells so that uniformly-sized three-dimensional cell structures are formed (abstract). Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to combine the teachings of modified Zhong et al. and Takahashi et al. to obtain the invention as specified in claim 5. Claim 7 is rejected under 35 U.S.C. 103 as being unpatentable over Zhong et al. (US 2010/0029000 A1) (already of record) in view of Kim et al. (WO 2021/215894 A1) (machine translated) (already of record) as applied to claim 6 above, and further in view of Tipgunlakant et al. (US 2014/0273191 A1) (already of record). Regarding claim 7, Zhong et al. discloses a culture device comprising a carrier (plate 20), a frame (sidewalls 14), and a driving mechanism (external pump 100), but does not disclose wherein the carrier and the frame are a semiconductor chip, and the driving mechanism is a piezoelectric micro-controller. However, Tipgunlakant et al. teaches that a semiconductor cell plate (para. 0172) and a piezoelectric actuator (para. 0365) are known in the art. Though modified Zhong et al. does not explicitly teach a semiconductor cell plate or a piezoelectric actuator, it would have been obvious to a person of ordinary skill in the art to use a semiconductor cell plate and a piezoelectric actuator because the substitution of one known element for another would have predictably resulted in a microfluidic system that monitors the biological properties of cells (para. 0172) and has controllable valves suitable for microfluidic systems (para. 0365), with reasonable expectation. Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to substitute the teachings of modified Zhong et al. with the teachings of Tipgunlakant et al. to obtain the invention as specified in claim 7. Claim 11 is rejected under 35 U.S.C. 103 as being unpatentable over Zhong et al. (US 2010/0029000 A1) (already of record) in view of Kim et al. (WO 2021/215894 A1) (machine translated) (already of record) as applied to claim 10 above, and further in view of Nguyen et al. (US 2023/0080802 A1) (already of record). Regarding claim 11, Zhong et al. discloses a culture device comprising insertion pipes comprising filtering holes (hole/slot/orifice 54), but does not disclose wherein the filtering holes of the at least one of the insertion pipes have an average aperture of less than 1 µm. However, Nguyen et al. teaches a membrane with an average pore size within a range of 0.2 µm to 10 µm (para. 0042) which allows cells of different sizes to be studied (para. 0042). It has been held that in the case where the claimed ranges overlap or lie inside ranges disclosed by the prior art, a prima facie case of obviousness exists (MPEP § 2144.05). The claimed range of an aperture less than 1 µm is obvious in view of Nguyen et al. because Nguyen et al. discloses a range of 0.2 µm to 10 µm, which overlaps the claimed range of less than 1 µm. It would have been obvious to one of ordinary skill in the art to select the claimed range from the broader prior art range because one of ordinary skill in the art would reasonably expect that a membrane with a pore size of 0.2 µm to 10 µm would allow cells of different sizes to be studied. Therefore, it would have been obvious to one of ordinary skill in the art before the effective filing date of the claimed invention to select the claimed range from the broader prior art range to obtain the invention as specified in claim 11. Conclusion THIS ACTION IS MADE FINAL. Applicant is reminded of the extension of time policy as set forth in 37 CFR 1.136(a). A shortened statutory period for reply to this final action is set to expire THREE MONTHS from the mailing date of this action. In the event a first reply is filed within TWO MONTHS of the mailing date of this final action and the advisory action is not mailed until after the end of the THREE-MONTH shortened statutory period, then the shortened statutory period will expire on the date the advisory action is mailed, and any nonprovisional extension fee (37 CFR 1.17(a)) pursuant to 37 CFR 1.136(a) will be calculated from the mailing date of the advisory action. In no event, however, will the statutory period for reply expire later than SIX MONTHS from the mailing date of this final action. Any inquiry concerning this communication or earlier communications from the examiner should be directed to ASHLEY LOPEZLIRA whose telephone number is (703)756-5517. The examiner can normally be reached Mon - Fri: 8:30-5:00. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Michael Marcheschi can be reached at 571-272-1374. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /ASHLEY LOPEZLIRA/Examiner, Art Unit 1799 /MICHAEL A MARCHESCHI/Supervisory Patent Examiner, Art Unit 1799
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Prosecution Timeline

Jun 27, 2023
Application Filed
Mar 20, 2026
Non-Final Rejection mailed — §103, §112
Jun 02, 2026
Response Filed
Jul 17, 2026
Final Rejection mailed — §103, §112 (current)

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Prosecution Projections

3-4
Expected OA Rounds
67%
Grant Probability
99%
With Interview (+48.4%)
3y 9m (~8m remaining)
Median Time to Grant
Moderate
PTA Risk
Based on 45 resolved cases by this examiner. Grant probability derived from career allowance rate.

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