Prosecution Insights
Last updated: April 19, 2026
Application No. 18/348,605

IMMUNOTHERAPEUTIC METHODS USING ELECTROPORATION

Final Rejection §103
Filed
Jul 07, 2023
Examiner
PEFFLEY, MICHAEL F
Art Unit
3794
Tech Center
3700 — Mechanical Engineering & Manufacturing
Assignee
VIRGINIA TECH INTELLECTUAL PROPERTIES, INC.
OA Round
4 (Final)
78%
Grant Probability
Favorable
5-6
OA Rounds
3y 7m
To Grant
90%
With Interview

Examiner Intelligence

Grants 78% — above average
78%
Career Allow Rate
1037 granted / 1334 resolved
+7.7% vs TC avg
Moderate +13% lift
Without
With
+12.6%
Interview Lift
resolved cases with interview
Typical timeline
3y 7m
Avg Prosecution
54 currently pending
Career history
1388
Total Applications
across all art units

Statute-Specific Performance

§101
1.0%
-39.0% vs TC avg
§103
36.8%
-3.2% vs TC avg
§102
28.3%
-11.7% vs TC avg
§112
14.6%
-25.4% vs TC avg
Black line = Tech Center average estimate • Based on career data from 1334 resolved cases

Office Action

§103
Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . Information Disclosure Statement The numerous references cited in the multiple Information Disclosure Statements have been afforded a cursory review, similar to what would be expected of a classification search of the prior art. Should there be any references of particular relevance to the instant application claims, applicant is respectfully requested to identify such references for further review by the examiner. Claim Rejections - 35 USC § 103 The following is a quotation of 35 U.S.C. 103 which forms the basis for all obviousness rejections set forth in this Office action: A patent for a claimed invention may not be obtained, notwithstanding that the claimed invention is not identically disclosed as set forth in section 102, if the differences between the claimed invention and the prior art are such that the claimed invention as a whole would have been obvious before the effective filing date of the claimed invention to a person having ordinary skill in the art to which the claimed invention pertains. Patentability shall not be negated by the manner in which the invention was made. Claim 21-33 and 35-45 are rejected under 35 U.S.C. 103(a) as being unpatentable over Davalos et al (2010/0030211) in view of the teaching of Har-Noy (2008/0112963). Regarding claim 21, Davalos et al provide a method for treating tissue comprising placing a probe in tissue, the probe having a first electrode (Figure 8b, for example). A plurality of electrical pulses are applied to tissue, the pulses having pulse parameters as claimed (see paragraphs [0104-0110], for example). The pulses are intended to generate a uniform electric field (para. [0044], for example) to cause cell death, and the treatment is also known to stimulate an immune response within the body (para. [0009], for example). Davalos et al also disclose that one or more exogenous or endogenous agents may be administered into tissue during treatment (Figure 9 and para. [0063], for example). Davalos et al also specifically teach a multi-modal treatment including the use of chemotherapeutic agents. See, for example, paragraph [0053]. In as much as newly added claim 45 indicates that administering chemotherapeutic agents will stimulate or otherwise modulate an immune system response within the body, the use of the same agents as disclosed by Davalos et al would obviously have the same effect. Additionally, Har-Noy disclose a method comprising the steps of ablating tissue with, for example, irreversible electroporation energy and then providing an agent to stimulate or otherwise modulate the immune response. See Abstract, for example. To have provided the Davalos et al method with the step of administering an agent specifically to stimulate or modulate the immune response within the body would have been an obvious consideration for one of ordinary skill in the art at the time of the invention, particularly since Davalos et al disclose the steps of administering chemotherapeutic agents in a multi-modality treatment and further since Har-Noy expressly teach it is known to administer agents to modulate the immune response of the body after irreversible electroporation. Regarding claim 22, there is necessarily a delay between pulses, and Davalos et al disclose a pulse length that may be less than 10 microseconds (para. [0105], for example). Regarding claim 23, Davalos et al disclose a frequency of pulse application of 0.001-100 Hz which would necessarily include a delay between pulses much longer than the pulse length (e.g. if using the smaller end of the pulse application frequency. Regarding claim 24, see paragraph [0110]. Regarding claim 25, the pulses are applied in bursts having a frequency of approximately 1Hz. See, also, paragraph [0039]. Regarding claim 26, IRE is well-known to alter the transmembrane potential of cells (para. [0009], for example). Regarding claim 27, Davalos et al provide a method for treating tissue comprising applying a plurality of biphasic pulses with a frequency, voltage and pulse length within the claimed range (para. [0104-0110]). There is inherently a delay between pulses, and the pulses cause irreversible disruption of a cellular membrane (e.g. IRE) in a first zone of tissue and also promotes an immune response (para. [0009]). Again, Davalos et al disclose using chemotherapeutic agents during the treatment, which agent is identified as being capable of stimulating or otherwise modulating the immune response within the body in applicant’s claim 45. Additionally, Har-Noy disclose a method comprising the steps of ablating tissue with, for example, irreversible electroporation energy and then providing an agent to stimulate or otherwise modulate the immune response. See Abstract, for example. To have provided the Davalos et al method with the step of administering an agent specifically to stimulate or modulate the immune response within the body would have been an obvious consideration for one of ordinary skill in the art at the time of the invention, particularly since Davalos et al disclose the steps of administering chemotherapeutic agents in a multi-modality treatment and further since Har-Noy expressly teach it is known to administer agents to modulate the immune response of the body after irreversible electroporation. Regarding claim 28, Davalos et al discuss different effects including IRE (abstract) and ECT (para. [0082]). Regarding claim 29, Davalos et al disclose a single burst duration within the claimed range (based on the disclosed number of pulses and pulse lengths disclosed in paragraphs [0104-0109]). Regarding claim 30, again Davalos et al disclose the use of pulse bursts having a pulse burst frequency (e.g. up to 100 Hz), each pulse burst necessarily comprising a pulse train. Regarding claim 31, Davalos et al disclose multiple bursts of pulse trains, and there is necessarily a delay between bursts. Regarding claims 32, 33 and 35, see again discussion of claims 22 and 23 and the Davalos et al frequency of pulse application which would provide such a delay. Regarding claims 36-38, Davalos et al disclose promoting an immune response by the application of a chemotherapeutic agent as addressed previously, which immune response would necessarily occur in the same areas given the use of similar parameters. Regarding claim 39, see paragraph [0107}, for example. Regarding claim 40, Davalos et al again disclose a method of treating tissue comprising generating a plurality of biphasic pulses in bursts to cause non-thermal ablation of cells. See paragraphs [0104-0112] which discloses the use of bursts having biphasic properties and parameters within the claimed ranges. See paragraph [0069] for the discussion of using a monopolar electrode. As addressed above, Davalos et al teach that IRE treatment is known to promote an immune response (para. [0009]) in treated tissue and further disclose the use of chemotherapeutic agents which are indicated to be an agent to promote an immune response as now recited in claim 45. Additionally, Har-Noy disclose a method comprising the steps of ablating tissue with, for example, irreversible electroporation energy and then providing an agent to stimulate or otherwise modulate the immune response. See Abstract, for example. To have provided the Davalos et al method with the step of administering an agent specifically to stimulate or modulate the immune response within the body would have been an obvious consideration for one of ordinary skill in the art at the time of the invention, particularly since Davalos et al disclose the steps of administering chemotherapeutic agents in a multi-modality treatment and further since Har-Noy expressly teach it is known to administer agents to modulate the immune response of the body after irreversible electroporation. Regarding claim 41, see again discussion of claim 23 and the delays that would result from using the smaller end of the frequency range. Regarding claims 42-43, see again paragraphs [0104-0111]. Regarding claim 44, Har-Noy specifically disclose administering monoclonal antibodies (para. [0042]). Regarding claim 45, see again paragraph [0053] of Davalos et al which discloses the use of chemotherapeutic agents. Also, Har-Noy disclose the use of several other agents that may be used to stimulate an immune response after irreversible electroporation. The exhaustive list of claim 45 is taken to mean that all the agents listed are obvious variants, particularly since there is no disclosure of criticality or unexpected results associated with any individual agent. Rather, applicant has merely provided a comprehensive list of known agents, and all agents are deemed to be within the purview of the skilled artisan. Response to Arguments Applicant’s arguments with respect to the pending claims have been considered but are moot because the new ground of rejection provides an additional reference to teach the newly claimed subject matter. Regarding the exhaustive list of agents provided in claim 45, the examiner again maintains such a listing would indicate the various agents are all obvious alternatives that would be within the purview of the skilled artisan. Again, there is no express disclosure of criticality or unexpected results associated with any of the agents, and no indication that any of the agents would be unknown to those of ordinary skill in the art. Further, the support for some of the agents seem questionable, particularly where applicant relies on the background section (e.g. paragraphs [0005] and [0009]) which recite prior art references that are not clear if the disclosed agents are being used in the same procedure of applying electrical pulses within the scope of the independent claims. Conclusion Applicant's amendment necessitated the new ground(s) of rejection presented in this Office action. Accordingly, THIS ACTION IS MADE FINAL. See MPEP § 706.07(a). Applicant is reminded of the extension of time policy as set forth in 37 CFR 1.136(a). A shortened statutory period for reply to this final action is set to expire THREE MONTHS from the mailing date of this action. In the event a first reply is filed within TWO MONTHS of the mailing date of this final action and the advisory action is not mailed until after the end of the THREE-MONTH shortened statutory period, then the shortened statutory period will expire on the date the advisory action is mailed, and any nonprovisional extension fee (37 CFR 1.17(a)) pursuant to 37 CFR 1.136(a) will be calculated from the mailing date of the advisory action. In no event, however, will the statutory period for reply expire later than SIX MONTHS from the mailing date of this final action. Any inquiry concerning this communication or earlier communications from the examiner should be directed to MICHAEL PEFFLEY whose telephone number is (571)272-4770. The examiner can normally be reached Mon-Fri 8 am-5 pm. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Linda Dvorak can be reached at (571) 272-4764. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /MICHAEL F PEFFLEY/Primary Examiner, Art Unit 3794 /M.F.P/ January 16, 2026
Read full office action

Prosecution Timeline

Jul 07, 2023
Application Filed
Nov 01, 2023
Response after Non-Final Action
Sep 04, 2024
Non-Final Rejection — §103
Dec 05, 2024
Response Filed
Jan 31, 2025
Final Rejection — §103
May 27, 2025
Interview Requested
Jun 04, 2025
Examiner Interview Summary
Jun 04, 2025
Applicant Interview (Telephonic)
Jul 07, 2025
Request for Continued Examination
Jul 09, 2025
Response after Non-Final Action
Jul 11, 2025
Non-Final Rejection — §103
Nov 13, 2025
Examiner Interview Summary
Nov 13, 2025
Applicant Interview (Telephonic)
Nov 14, 2025
Response Filed
Jan 16, 2026
Final Rejection — §103 (current)

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Study what changed to get past this examiner. Based on 5 most recent grants.

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Prosecution Projections

5-6
Expected OA Rounds
78%
Grant Probability
90%
With Interview (+12.6%)
3y 7m
Median Time to Grant
High
PTA Risk
Based on 1334 resolved cases by this examiner. Grant probability derived from career allow rate.

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