Prosecution Insights
Last updated: April 19, 2026
Application No. 18/540,517

BIOMARKERS FOR EARLY DIAGNOSIS AND DIFFERENTIATION OF MYCOBACTERIAL INFECTION

Non-Final OA §112
Filed
Dec 14, 2023
Examiner
SALMON, KATHERINE D
Art Unit
1682
Tech Center
1600 — Biotechnology & Organic Chemistry
Assignee
Wisconsin Alumni Research Foundation
OA Round
3 (Non-Final)
42%
Grant Probability
Moderate
3-4
OA Rounds
3y 11m
To Grant
80%
With Interview

Examiner Intelligence

Grants 42% of resolved cases
42%
Career Allow Rate
329 granted / 776 resolved
-17.6% vs TC avg
Strong +38% interview lift
Without
With
+38.0%
Interview Lift
resolved cases with interview
Typical timeline
3y 11m
Avg Prosecution
105 currently pending
Career history
881
Total Applications
across all art units

Statute-Specific Performance

§101
18.3%
-21.7% vs TC avg
§103
27.9%
-12.1% vs TC avg
§102
13.2%
-26.8% vs TC avg
§112
33.7%
-6.3% vs TC avg
Black line = Tech Center average estimate • Based on career data from 776 resolved cases

Office Action

§112
DETAILED ACTION Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . A request for continued examination under 37 CFR 1.114, including the fee set forth in 37 CFR 1.17(e), was filed in this application after final rejection. Since this application is eligible for continued examination under 37 CFR 1.114, and the fee set forth in 37 CFR 1.17(e) has been timely paid, the finality of the previous Office action has been withdrawn pursuant to 37 CFR 1.114. Applicant's submission filed on 9/29/2025 has been entered. Applicant’s election without traverse of Group I and LOC108634521 in the reply filed on 9/25/2024 is acknowledged. Claims 1-2, 4-21 are pending. Claim 3 is cancelled. Claims 7-20 are withdrawn as being drawn to a nonelected invention. The following rejections are modified with response to arguments following or newly applied (35 USC 112b). This action for claims 1-2 and 4-6, 21 is NONFINAL. Claim Rejections - 35 USC § 112 The following is a quotation of the first paragraph of 35 U.S.C. 112(a): (a) IN GENERAL.—The specification shall contain a written description of the invention, and of the manner and process of making and using it, in such full, clear, concise, and exact terms as to enable any person skilled in the art to which it pertains, or with which it is most nearly connected, to make and use the same, and shall set forth the best mode contemplated by the inventor or joint inventor of carrying out the invention. The following is a quotation of the first paragraph of pre-AIA 35 U.S.C. 112: The specification shall contain a written description of the invention, and of the manner and process of making and using it, in such full, clear, concise, and exact terms as to enable any person skilled in the art to which it pertains, or with which it is most nearly connected, to make and use the same, and shall set forth the best mode contemplated by the inventor of carrying out his invention. Claims 1-2 and 4-6, 21 are rejected under 35 U.S.C. 112(a) or 35 U.S.C. 112 (pre-AIA ), first paragraph, as failing to comply with the written description requirement. The claim(s) contains subject matter which was not described in the specification in such a way as to reasonably convey to one skilled in the relevant art that the inventor or a joint inventor, or for applications subject to pre-AIA 35 U.S.C. 112, the inventor(s), at the time the application was filed, had possession of the claimed invention. The claims are drawn administering antibiotics based upon determining differential expression of LOC1088634521. Therefore the claims encompass administering based upon expression level however, the specification does not describe administering in any ruminant mammal as compared to a naïve ruminant mammal. In particular the specificiaotn has not limited the definition of “ruminant mammal” in such a way that it would not encompass “nave ruminant mammals”. The specification, however, appears to determine antibiotic treatment in goats infected with mycobacteria and not as claimed any “ruminant mammal”. The specification discloses in table 1 that host of goat and cow that genes can differentiate infected from naïve animals (p. 15). The table discloses LOC108634521 is upregulated in infected animals versus naïve animals. However, the specification does not provide that any differential expression in any sample from any ruminant mammals functionally detect infection. Furhtermore, as provided below LOC108634521 appears to be derived from goat, however, the specification does not appear to provide support that this structure is observed in cows, rather the table does not indicate that these expression profiles are from both goat and cow or “goat or cow”. Therefore the specification does not provide description of the breath of the claims. The art (NCBI LOC108634521 downloaded 10/25/2024) teaches that this structures is from goat organism, however, there is no indication that this same structure is found within cow or any other mammal. Further, the art does not provide guidance that expression of LOC108634521 provides that one can treat in any ruminant mammal. The art does not provide the critical structural elements for the asserted functionality. In particular Enard et al. (Science 2002 Vol 296 p. 340 cited on IDS) teaches that even between closely related species gene expression patterns differ (abstract). Enard et al. teaches that mRNA expression levels are different between humans, chimpanzees, orangutans and rhesus marcques (p. 340 1st column last sentence-2nd column 1st paragraph). Enard et al. teaches that there are a large number of quantitative differences in gene expression in closely related mammals (p. 342 2nd column last paragraph). Therefore the art teaches that even between very closely related mammals there is a divergence of gene expression. As such Enard et al. teaches that merely being a biomarker in one species would not be sufficient to provide support for the structure in other species. The claims encompass any sample. The art of Cobb et al (Crit Care Med 2002 Vol. 30 p. 2711, newly applied) teaches the unpredictability in analysis of gene expression in spleen and liver sample from septic mice. Notably, the reference teaches that, when compared to a non-septic sample, the relevant expression profiles of the septic mouse spleen and the septic mouse liver contain different nucleic acids at different levels (Table 1; p.2714, middle col., lns.2-8). As such the art teaches that expression levels of the same nucleic acids in different tissue samples differ. Therefore the art indicates that an association of expression level to a disease in one sample would not be described to an association to any other sample type. Accordingly, the specification has not provided the critical elements needed in the structure to predictive functionally. Therefore the specification lacks written description of any subject representative of the broadly claimed genus. In analysis of the claims for compliance with the written description requirement of 35 U.S.C. 112, first paragraph, the written description guidelines note regarding genus/species situations that “Satisfactory disclosure of a ``representative number'' depends on whether one of skill in the art would recognize that the applicant was in possession of the necessary common attributes or features of the elements possessed by the members of the genus in view of the species disclosed.” (See: 'Written Description" Requirement, Federal Register, Vol. 66, No. 4, pages 1099-1111, Friday January 5, 2001.) Response to Arguments The reply traverses the rejection. A summary of the arguments is provided below with response to arguments following. The reply asserts that the claims have been amended to increased expression and infection with mycobacterium (p. 7). This amendment has removed some issues set forth above with regard to naïve and expression patterns, however, the description has been maintained based upon the breadth of the claims. The reply asserts that there is at lest 90% homology of LOC108634521 and ruminant animals (p. 7). The reply asserts that as the specification disclose LOC108634521 and homology and that the claims only requires detection of expression. These arguments have been reviewed but have not been found persuasive. The claims as amended require antibiotic administration based upon increased expression of LOC108634521 or a homology of 90%. However, the specification has not described these homologies or LOC108634521 in any ruminant animal and the functionality of administering antibiotics. Although the skilled artisan could determine the structure that would represent 90% homology and the structure of LOC1086534521 the specification does not provide that increased expression of these structures are functionally determined in any ruminant species. Furthermore the specification does not describe that increased expression functionally is associated with provided antibiotics. Therefore the rejection provided above is maintained. Newly Applied Claim Rejections - 35 USC § 112 The following is a quotation of 35 U.S.C. 112(b): (b) CONCLUSION.—The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the inventor or a joint inventor regards as the invention. The following is a quotation of 35 U.S.C. 112 (pre-AIA ), second paragraph: The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the applicant regards as his invention. Claims 1-2 and 4-6, 21 are rejected under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA ), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention. Claims 1-2 and 4-6 are indefinite over “the subject ruminant mammal with increased expression of LOC108634521 as compared to the sample from the naïve ruminant mammal is infected with a mycobacterium”. The phrase is unclear as it is not clear if the claim is intending that the subject ruminant is a mycobacterium infected subject ruminant or if the claim intends that the detection of expression detects that the subject ruminant is infected with mycobacterium. Claims 2 is unclear as it appears the claim 1 subject ruminant has mycobacterial infection and as such it is not clear how claim 2 limits claim 1. Conclusion No claims are allowed. Any inquiry concerning this communication or earlier communications from the examiner should be directed to KATHERINE D SALMON whose telephone number is (571)272-3316. The examiner can normally be reached 9-530. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Wu Cheng (Winston) Shen can be reached on 5712723157. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /KATHERINE D SALMON/Primary Examiner, Art Unit 1682
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Prosecution Timeline

Dec 14, 2023
Application Filed
Oct 28, 2024
Non-Final Rejection — §112
Feb 28, 2025
Response Filed
Mar 28, 2025
Final Rejection — §112
Sep 29, 2025
Request for Continued Examination
Oct 06, 2025
Response after Non-Final Action
Dec 15, 2025
Non-Final Rejection — §112 (current)

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Study what changed to get past this examiner. Based on 5 most recent grants.

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Prosecution Projections

3-4
Expected OA Rounds
42%
Grant Probability
80%
With Interview (+38.0%)
3y 11m
Median Time to Grant
High
PTA Risk
Based on 776 resolved cases by this examiner. Grant probability derived from career allow rate.

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