Prosecution Insights
Last updated: April 19, 2026
Application No. 18/549,333

METHOD FOR DETECTING OR QUANTIFYING PHOTOAGED CELLS, APPLICATION OF SAME, AND METHOD FOR PREPARING PHOTOAGED CELLS

Non-Final OA §102§112
Filed
Sep 06, 2023
Examiner
DAUNER, JOSEPH G
Art Unit
1682
Tech Center
1600 — Biotechnology & Organic Chemistry
Assignee
USHIO DENKI KABUSHIKI KAISHA
OA Round
1 (Non-Final)
57%
Grant Probability
Moderate
1-2
OA Rounds
3y 4m
To Grant
91%
With Interview

Examiner Intelligence

Grants 57% of resolved cases
57%
Career Allow Rate
404 granted / 712 resolved
-3.3% vs TC avg
Strong +35% interview lift
Without
With
+34.7%
Interview Lift
resolved cases with interview
Typical timeline
3y 4m
Avg Prosecution
76 currently pending
Career history
788
Total Applications
across all art units

Statute-Specific Performance

§101
11.1%
-28.9% vs TC avg
§103
27.4%
-12.6% vs TC avg
§102
18.4%
-21.6% vs TC avg
§112
30.1%
-9.9% vs TC avg
Black line = Tech Center average estimate • Based on career data from 712 resolved cases

Office Action

§102 §112
DETAILED ACTION Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . The claims dated 10/19/2023 are under consideration. Priority The present application is a 371 national stage entry of PCT/JP2022/008772 (filed 3/2/2022), and claims benefit of JAPAN 2021-037357 (filed 3/9/2021). An English translation of the certified foreign priority document has not been received. Information Disclosure Statement The listing of references in the specification or the citation of references throughout the specification is not a proper information disclosure statement. 37 CFR 1.98(b) requires a list of all patents, publications, or other information submitted for consideration by the Office, and MPEP § 609.04(a) states, "the list may not be incorporated into the specification but must be submitted in a separate paper." Therefore, unless the references have been cited by the examiner on form PTO-892 or cited on a submitted IDS, they have not been considered. Specification The disclosure is objected to because of the following informalities: the specification recites “Cerabeam T” in paragraph 76. It appears it may have been intended to recite “CerabeamTM”. On page 27, the specification recites “LONZAKK” rather than “LONZA”, as was done on page 26. On page 28, the specification recites “TRIzol TM” rather than “TRIzolTM”. Appropriate correction is required. Claim Rejections - 35 USC § 112 The following is a quotation of 35 U.S.C. 112(b): (b) CONCLUSION.—The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the inventor or a joint inventor regards as the invention. The following is a quotation of 35 U.S.C. 112 (pre-AIA ), second paragraph: The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the applicant regards as his invention. Claims 1-3 and 5 are rejected under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA ), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention. Regarding claim 1, it is not clear how the recited preamble is intended to breathe life and meaning into the claims. The preamble of the claim recites a “method for detecting or quantifying a photoaged cell”. However, the method steps in the body of the claim only require a single step of detecting or quantifying expression of at least one gene selected from the group consisting of GPR17, CD34, GABRR1, OR2AG2, CMKLR1, CDH19, CD93, AVPR2, CCR7, and OXGR1 in a cell. Thus, it is unclear if applicant intends to cover any method of detecting or quantifying expression of at least one gene selected from the group consisting of GPR17, CD34, GABRR1, OR2AG2, CMKLR1, CDH19, CD93, AVPR2, CCR7, and OXGR1 in a cell, or if the method is intended to somehow require more to accomplish the goal set forth in the preamble. If it is the later, then it appears that the claims are incomplete, as they fail to provide any active steps that clearly accomplish the goal of detecting or quantifying a photoaged cell as set forth by the preamble of the claim. Amending the claim to include an active process step directed towards detecting or quantifying photoaged cells based on the detected or quantified expression of the at least one gene may overcome this objection. Claim 2 similarly is indefinite because it requires performing the method of claim 1. Regarding claim 1, the claim has the intended use of “quantifying a photoaged cell”, but the claim requires detecting or quantifying expression “in a cell”. It is unclear how to quantify a cell when only a single cell is analyzed. Claim 2 is similarly indefinite because it requires performing the method of claim 1. Regarding claim 3, the claim has the recited intended use of screening a substance that “reduces a photoaged cell” and “selecting” such a substance. It is unclear what is intended by or encompassed by a substance that “reduces” a photoaged cell or any type of cell, for that matter. For example, to what is the cell to be “reduced”? Regarding claim 5, the claim recites “a cell that has been irradiated with light having an emission peak within a wavelength range of 300 to 315 nm”. Based on the use of the passive voice, it is unclear if the claim is intended to include an active method step of “irradiating a cell with light having an emission peak within a wavelength range of 300 to 315 nm” or if the claim broadly encompasses any cell having the structure resulting from being irradiated by such light. Claim Rejections - 35 USC § 102 In the event the determination of the status of the application as subject to AIA 35 U.S.C. 102 and 103 (or as subject to pre-AIA 35 U.S.C. 102 and 103) is incorrect, any correction of the statutory basis (i.e., changing from AIA to pre-AIA ) for the rejection will not be considered a new ground of rejection if the prior art relied upon, and the rationale supporting the rejection, would be the same under either status. The following is a quotation of the appropriate paragraphs of 35 U.S.C. 102 that form the basis for the rejections under this section made in this Office action: A person shall be entitled to a patent unless – (a)(1) the claimed invention was patented, described in a printed publication, or in public use, on sale, or otherwise available to the public before the effective filing date of the claimed invention. Claim(s) 1-3 and 5 is/are rejected under 35 U.S.C. 102(a)(1) as being anticipated by Mittelbrunn (J Invest Dermatol. 2005. 125:334-342). Claim 1 is drawn to a “method for detecting or quantifying a photoaged cell”; however, the active method steps do not explicitly require an active method step of detecting or quantifying any type of cell. MPEP 2111.02 states: If the body of a claim fully and intrinsically sets forth all of the limitations of the claimed invention, and the preamble merely states, for example, the purpose or intended use of the invention, rather than any distinct definition of any of the claimed invention's limitations, then the preamble is not considered a limitation and is of no significance to claim construction. Accordingly, the claim language of "method for detecting or quantifying a photoaged cell" merely sets forth the intended use or purpose of the claimed methods, but does not limit the scope of the claims. The claims are given the broadest reasonable interpretation as requiring a single step of: detecting or quantifying expression of at least one gene selected from the group consisting of GPR17, CD34, GABRR1, OR2AG2, CMKLR1, CDH19, CD93, AVPR2, CCR7, and OXGR1 in a cell. PNG media_image1.png 168 700 media_image1.png Greyscale Regarding claim 1, Mittelbrunn teaches detecting the expression of CCR7 in a cell as depicted in Figure 9, reproduced below: Mittelbrunn anticipates claim 1 because it teaches all of the active method steps required by the claim. Claim 2 recites an active method step of “performing the detecting or quantifying according to claim 1”. The method of claim 1 is interpreted as described above. The first active method step of claim 2 is interpreted as requiring a single step of: detecting or quantifying expression of at least one gene selected from the group consisting of GPR17, CD34, GABRR1, OR2AG2, CMKLR1, CDH19, CD93, AVPR2, CCR7, and OXGR1 in a cell. Regarding claim 2, Mittelbrunn teaches detecting the expression of CCR7 in a cell as depicted in Figure 9, reproduced above. Mittelbrunn further teaches using a Flow cytometry analysis in which cells are analyzed and “sorted” based on fluorescence intensity (p. 341, Flow cytometry analysis). Mittelbrunn anticipates claim 2 because it teaches all of the active method steps required by the claim. Regarding claim 3, Mittelbrunn teaches bringing cells into contact with LPS as a “candidate substance” (Figure 9; and p. 341, Preparation of monocyte-derived DC). Mittelbrunn teaches detecting the expression of CCR7 in a cell contacted with LPS as depicted in Figure 9, reproduced above. Mittelbrunn identifies or “selects” LPS as a substance that suppresses development of a photoaged cell because LPS induces CCR7 expression, which irradiation suppressed as depicted in Fig. 9 reproduced above. Claim 5 is drawn to a “method for preparing a photoaged cell”; however, the active method steps do not explicitly require an active method step of preparing, treating or manipulating any type of cell. MPEP 2111.02 states: If the body of a claim fully and intrinsically sets forth all of the limitations of the claimed invention, and the preamble merely states, for example, the purpose or intended use of the invention, rather than any distinct definition of any of the claimed invention's limitations, then the preamble is not considered a limitation and is of no significance to claim construction. Accordingly, the claim language of "method for preparing a photoaged cell" merely sets forth the intended use or purpose of the claimed methods, but does not limit the scope of the claims. The claims are given the broadest reasonable interpretation as requiring a single step of: detecting or quantifying expression of at least one gene selected from the group consisting of GPR17, CD34, GABRR1, OR2AG2, CMKLR1, CDH19, CD93, AVPR2, CCR7, and OXGR1 in a cell that has been irradiated with light having an emission peak within a wavelength range of 300 to 315 nm. The type of cell analyzed in the step is limited to those that have the structure resulting from irradiation with the specified type of light; however, the claim does not require an active method step of irradiating the cell. Regarding claim 5, Mittelbrunn teaches detecting the expression of CCR7 in a cell as depicted in Figure 9, reproduced above, in cells that have been irradiated with UVB (p. 341, Solar-simulated UV radiation) having a peak wavelength of 290-320 nm (p. 334). The claimed wavelengths of light are encompassed by those used by Mittelbrunn. Conclusion No claims allowed. Any inquiry concerning this communication or earlier communications from the examiner should be directed to JOSEPH G DAUNER whose telephone number is (571)270-3574. The examiner can normally be reached 7 am EST to 4:30 EST with second Fridays Off. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Wu-Cheng Winston Shen can be reached at 5712723157. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /JOSEPH G. DAUNER/Primary Examiner, Art Unit 1682
Read full office action

Prosecution Timeline

Sep 06, 2023
Application Filed
Jan 01, 2026
Non-Final Rejection — §102, §112
Mar 12, 2026
Interview Requested
Mar 19, 2026
Applicant Interview (Telephonic)
Mar 21, 2026
Examiner Interview Summary

Precedent Cases

Applications granted by this same examiner with similar technology

Patent 12601006
TARGETED, LONG-READ NUCLEIC ACID SEQUENCING FOR THE DETERMINATION OF CYTOSINE MODIFICATIONS
2y 5m to grant Granted Apr 14, 2026
Patent 12595506
Compositions and Methods for Analyzing Modified Nucleotides
2y 5m to grant Granted Apr 07, 2026
Patent 12584162
HYDROXYMETHYLATION ANALYSIS OF CELL-FREE NUCLEIC ACID SAMPLES FOR ASSIGNING TISSUE OF ORIGIN, AND RELATED METHODS OF USE
2y 5m to grant Granted Mar 24, 2026
Patent 12571042
MARKERS SPECIFIC FOR PLURIPOTENT STEM CELLS, AND METHODS OF USING THE SAME
2y 5m to grant Granted Mar 10, 2026
Patent 12565682
METHODS OF TREATING CANCER
2y 5m to grant Granted Mar 03, 2026
Study what changed to get past this examiner. Based on 5 most recent grants.

AI Strategy Recommendation

Get an AI-powered prosecution strategy using examiner precedents, rejection analysis, and claim mapping.
Powered by AI — typically takes 5-10 seconds

Prosecution Projections

1-2
Expected OA Rounds
57%
Grant Probability
91%
With Interview (+34.7%)
3y 4m
Median Time to Grant
Low
PTA Risk
Based on 712 resolved cases by this examiner. Grant probability derived from career allow rate.

Sign in with your work email

Enter your email to receive a magic link. No password needed.

Personal email addresses (Gmail, Yahoo, etc.) are not accepted.

Free tier: 3 strategy analyses per month