Notice of Pre-AIA or AIA Status
The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA .
Claim Status
Claims 50-55 and 57-73 are pending and examined. Claims 1-49 and 56 have been cancelled.
Specification
The objections to the specification are withdrawn in light of the amendments.
Claim Objections
Claim 64 is objected to for failing to recite a transition between each of the wherein clauses.
Claim Rejections - 35 USC § 112
The following is a quotation of 35 U.S.C. 112(b):
(b) CONCLUSION.—The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the inventor or a joint inventor regards as the invention.
The following is a quotation of 35 U.S.C. 112 (pre-AIA ), second paragraph:
The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the applicant regards as his invention.
Claims 50-55, 57, 58 and 70-73 are rejected under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA ), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention.
Claim 50 is drawn to an enzyme operably linked to a promoter where said promoter is configured to control downstream gene transcription. The metes and bounds of the claim are indefinite because it is not clear if the promoter controls expression of the enzyme or a different gene.
Claim 52 recites the limitation “wherein said TSWV comprising the S, M, and L genome segments”. The metes and bounds of the claim are indefinite because it is not clear if the tospovirus vector that is derived from TSWV comprises these genomic segments, or, if the claim is merely reciting what TSWV comprises.
In other words, the tospovirus vector as encompassed by claim 52 is not required to comprise the S, M and L genome segments.
The lack of clarity arises from the fact that claim 50 is drawn to “a polynucleotide sequence” of tospovirus vector yet none of the claims which depend from claim 50 specifically limit the structure of this polynucleotide sequence.
To remedy this issue it is suggested that claim 50 a) either be directed to a tospovirus vector, or, claims which depend from claim 50 should reflect that it is the structure of the polynucleotide sequence of claim 50 a) which is being defined.
To expedite prosecution the claims are interpreted to mean that the polynucleotide sequence of part a) comprises sequences from a tospovirus vector, and in particular, sequences from TSWV.
Claim 55 recites the limitation “chimeric”: there is insufficient antecedent basis for this claim limitation.
Claim 57 is drawn to a tospovirus vector comprising nucleic acid constructs comprising the viral polynucleotide sequences of said tospovirus vector. The metes and bounds of the claim are indefinite because it is not clear how the claim is further limited as it appears that the tospovirus vector merely comprises itself.
Claim 58 is drawn to constructs comprising L, M and S genomic segments while the claim from which it depends is drawn to “chimeric” S, M and L genome segments. Thus, the metes and bounds of the claim are indefinite because it is not clear if the segments are chimeric or not.
The metes and bounds of claim 61 are indefinite because the method further comprises obtaining a virus-free plant through steps a), b) and c): it is not clear how these steps further limit the claim from which they depend as they appear to be method steps unrelated to claim 60 and because these steps fail to modify the genetic material of the plant.
This position is reiterated by the fact that claim 61 (b) recites infected by “a” tospovirus: it is not clear if this refers to the tospovirus vector of claim 60 or if it refers to a different tospovirus vector.
Claim 71 is drawn to a kit comprising a polynucleotide sequence of a tospovirus and at least a polynucleotide encoding a nucleic acid modifying enzyme and that comprises a construct comprising TSWV S, M and L genome segments comprising a nucleic acid modifying enzyme and helper expression vectors.
The metes and bounds of the claim are indefinite because it is not clear if the kit comprises the same nucleic acid modifying enzyme as encompassed by claim 50 or a different one. In other words, it is not clear if the limitations of claim 71 are additional and separate limitations, or if they are intended to further define virus vector system of claim 50.
Claims 51, 53, 54, 72 and 73 are rejected for depending upon a rejected base claim and for failing to remedy the issues of indefiniteness.
Response to Arguments
Applicant traverses the rejection of claim 52 because it is clear TSWV sequences are being recited (Applicant reply dated 17 November 2025, p. 14).
This argument is not persuasive. The office does not contest the claim recites TSWV sequences. The issue is whether the tospovirus vector, in fact, comprises the sequences, or, if the claim is merely reciting that TSWV comprises the sequences.
The following is a quotation of the first paragraph of 35 U.S.C. 112(a):
(a) IN GENERAL.—The specification shall contain a written description of the invention, and of the manner and process of making and using it, in such full, clear, concise, and exact terms as to enable any person skilled in the art to which it pertains, or with which it is most nearly connected, to make and use the same, and shall set forth the best mode contemplated by the inventor or joint inventor of carrying out the invention.
The following is a quotation of the first paragraph of pre-AIA 35 U.S.C. 112:
The specification shall contain a written description of the invention, and of the manner and process of making and using it, in such full, clear, concise, and exact terms as to enable any person skilled in the art to which it pertains, or with which it is most nearly connected, to make and use the same, and shall set forth the best mode contemplated by the inventor of carrying out his invention.
Claims 50-55 and 57-72 REMAIN rejected, and claim 73 is NOW rejected, under 35 U.S.C. 112(a) or 35 U.S.C. 112 (pre-AIA ), first paragraph, as failing to comply with the written description requirement. The claim(s) contains subject matter which was not described in the specification in such a way as to reasonably convey to one skilled in the relevant art that the inventor or a joint inventor, or for applications subject to pre-AIA 35 U.S.C. 112, the inventor(s), at the time the application was filed, had possession of the claimed invention.
Instant claims 50-55 and 57-73 are broadly drawn to a recombinant virus vector system comprising a tospovirus and at least a polynucleotide sequence encoding a sequence specific nucleic acid modifying enzyme that targets a genomic sequence in a plant cell, wherein the modification enzyme is a CRISPR/Cas nuclease and wherein the tospovirus is TSWV and comprises S, M and L genome segments wherein said segments are nucleic acid sequences having as little as 70% sequence identity to SEQ ID NO: 1, 2 and 3, respectively, wherein the S and/or M segments comprise a heterologous polynucleotide sequence encoding a component of the sequence specific nucleic acid modifying enzyme, wherein the segments are in molar ratios of 1~2:2~10:1~2, plants produced from said system, a method for modifying the genetic material of a plant cell by using said system, and a kit comprising said system.
The specification describes that all three TSWV S, M and L genome segments are needed for expression of recombinant genes in plant cells (e.g., see Example 2 and p. 49). However, the specification also describes that the M genomic segment is not suitable for rescue of recombinantly expressed Cas12a-crRNA (p. 52, last ¶). Moreover, the specification describes that it is a specific ratio of S, M and L genome segments that is required for functional TSWV-Cas12a-crRNA (p. 52, last ¶).
The written description requirement may be satisfied through sufficient description of a representative number of species by disclosing relevant and identifying characteristics such as structural or other physical and/or chemical properties, by disclosing functional characteristics coupled with a known or disclosed correlation between function and structure, or by a combination of such identifying characteristics, sufficient to show the applicant was in possession of the invention as claimed. See Eli Lilly,119 F.3d at 1568, 43 USPQ2d at 1406.
In the instant matter, the claims encompass a vast genus of viral vectors not limited to those derived from TSWV yet the specification only describes a single embodiment of the invention: a TSWV vector designed using the nucleic acid sequences of SEQ ID NO: 1, 2 and 3 as opposed to nucleic acid sequences having as little as 70% sequence identity to SEQ ID NO: 1, 2 and 3.
Here, and for example, because claim 52 encompasses nucleic acid sequences having as little as 70% sequence identity to SEQ ID NO: 1, the tospovirus vector of claim 50 encompasses a polynucleotide sequence having 890 nucleic acid substitutions relative to SEQ ID NO: 1 and would encompass 3890 distinct gene variants.
However, without describing where in the sequence of SEQ ID NO: 1 such variations can be sustained (or SEQ ID NO: 2 and 3), one would not be led to believe that Applicant was in possession of the genus of polynucleotide sequences that retain tospovirus functional activity to express a nucleic acid modifying enzyme to target or modify genomic sequences in a plant cell.
This description is paramount in light of the state of the art which describes that that reconstitution of NSVs in planta is particularly difficult as all constructs need to be delivered in to the same plant cell, because the RdRp of TSWV is exceptionally large making it difficult to express, and because achieving the proper ratios of all three genome segments is not easy or consistent (Feng et al, 2020, PNAS, 117:1181-1190; see p. 1182, col. 1, ¶ 3; see also p. 1187, col. 2, ¶ 1).
Feng et al further describe that S antigenomic, M genomic and L antigenomic DNA of TSWV wherein the M genomic DNA is codon optimized results in systemic infection and viral rescue whereas the nonstructural protein of the S segment (NS) interfered with rescue of genomic RNA (see Abstract; see also p. 1184, col. 1, ¶ 2 bridging p. 1185; see also p. 1188, col. 2, last ¶).
Moreover, Feng et al describe that particular promoters should be used in order to successfully express the aforementioned minireplicon system (e.g., see p. 1182, col. 2, penultimate ¶; see also p. 1186, col. 2, last ¶). Here, the specification suggests that any promoter may be used but only describes, in fact, the use of a double 35S promoter (e.g., see Figure 3).
Thus, the art makes clear that it is not any promoter or polynucleotide sequence of a tospovirus that permits expression of a sequence of interest. Rather, it is a specific promoter or species of polynucleotides of TSWV that permits expression of a sequence of interest in a plant cell.
The Federal Circuit has clarified the application of the written description requirement. The court stated that a written description of an invention "requires a precise definition, such as by structure, formula, [or] chemical name, of the claimed subject matter sufficient to distinguish it from other materials." University of California v. Eli Lilly and Co., 119 F.3d 1559, 1568; 43 USPQ2d 1398, 1406 (Fed. Cir. 1997).
The court also concluded that "naming a type of material generally known to exist, in the absence of knowledge as to what that material consists of, is not a description of that material." Id.
Further, the court held that to adequately describe a claimed genus, Patent Owner must describe a representative number of the species of the claimed genus, and that one of skill in the art should be able to "visualize or recognize the identity of the members of the genus." Id. See MPEP 2163.
Applicant should note that the written description requirement serves to warn an innocent purchaser of the infringement of a patent, and conversely requires the patentee to distinguish the invention in the disclosure, and thus prevents the inventor from practicing upon the credulity or fears of other persons or from pretending that the invention is more than what it is. see Vas-Cath Inc. v. Mahurkar 1991 (CA FC) 19 USPQ2d 1111, 1115.
Here, the instant specification has merely described that a particular set of TSWV polynucleotide sequence may introduce a sequence specific nucleic acid modifying enzyme, and has failed to describe any other polynucleotide sequence of a tospovirus that would persuade a skilled artisan that Applicant was in possession of the genus of virus vector systems, methods of using said system and kits comprising said system as broadly claimed.
As such, these claims are “reach through” claims in which Applicant has only described a starting material and at least one method step, but has not described the resulting product such that the genus of products that can be produced by the recited method steps and materials is so large that one of skill in the art would not readily envision the members of the claimed genus. (See Univ. of Rochester v. G.D. Searle & Co., 358 F.3d 916, 920-23, 69 USPQ2d 1886, 1890-93 (Fed. Cir. 2004)).
Therefore, in light of the extreme breadth of the claims, the failure of the specification to describe a representative number of species from the broad genus of tospovirus polynucleotide sequences, the lack of working examples and the state of the art which describes that specific TSWV polynucleotide sequences are required to express a polynucleotide of interest, the skilled artisan would not be of the opinion that Applicant was in possession of the invention as broadly claimed.
Response to Arguments
Applicant traverses the rejection of the claims by asserting the working examples demonstrate possession of the instant invention and that viruses of the genus tospovirus have similar particle morphology, genome structure, replication strategy and molecular characteristics (Applicant reply dated 17 November 2025, p. 15).
These arguments are not persuasive and fail to address the rejection as set forth above. In the instant matter, the claims encompass a vast genus of viral vectors not limited to those derived from TSWV yet the specification only describes a single embodiment of the invention: a TSWV vector designed using the nucleic acid sequences of SEQ ID NO: 1, 2 and 3 as opposed to nucleic acid sequences having as little as 70% sequence identity to SEQ ID NO: 1, 2 and 3.
Here, and for example, because claim 52 encompasses nucleic acid sequences having as little as 70% sequence identity to SEQ ID NO: 1, the tospovirus vector of claim 50 encompasses a polynucleotide sequence having 890 nucleic acid substitutions relative to SEQ ID NO: 1 and would encompass 3890 distinct gene variants.
However, without describing where in the sequence of SEQ ID NO: 1 such variations can be sustained (or SEQ ID NO: 2 and 3), one would not be led to believe that Applicant was in possession of the genus of polynucleotide sequences that retain tospovirus functional activity to express a nucleic acid modifying enzyme to target or modify genomic sequences in a plant cell.
In fact, Applicant’s own arguments support this position: the understanding of the art is that it is difficult to express the entire CRISPR/Cas system from a vector system (Applicant reply dated 15 November 2025, p. 18, ¶ 1; see also p. 19, last ¶). Thus, if Applicant’s position is accepted then the skilled artisan would certainly not be of the opinion that Applicant possesses virus vector systems comprising any conceivable polynucleotide sequence from the exhaustive genus of tospovirus vector as broadly claimed.
In other words, Applicant’s position is not commensurate in scope with what is claimed because is it is a specific combination of sequences that provide the alleged unexpected results rather than any possible tospovirus vector providing said results.
Claim Rejections - 35 USC § 103
In the event the determination of the status of the application as subject to AIA 35 U.S.C. 102 and 103 (or as subject to pre-AIA 35 U.S.C. 102 and 103) is incorrect, any correction of the statutory basis (i.e., changing from AIA to pre-AIA ) for the rejection will not be considered a new ground of rejection if the prior art relied upon, and the rationale supporting the rejection, would be the same under either status.
The following is a quotation of 35 U.S.C. 103 which forms the basis for all obviousness rejections set forth in this Office action:
A patent for a claimed invention may not be obtained, notwithstanding that the claimed invention is not identically disclosed as set forth in section 102, if the differences between the claimed invention and the prior art are such that the claimed invention as a whole would have been obvious before the effective filing date of the claimed invention to a person having ordinary skill in the art to which the claimed invention pertains. Patentability shall not be negated by the manner in which the invention was made.
Claim(s) 50-55 and 57-72 REMAIN rejected, and claim 73 is NOW rejected, under 35 U.S.C. 103 as being unpatentable over Harling et al (Patent No. US 11,492,630 B2) and Cody et al (2017, Plant Physiology, 175:23-35) and Ali et al (2015, Molecular Plant 8, 1288-1291) in view of Feng et al (2020, PNAS, 117:1181-1190) and Hu et al (2011, Arch Virol., 156(10)1905-1908) and in further view of Fazio et al (1980, Archives of Virology, 63:305-309).
The factual inquiries for establishing a background for determining obviousness under 35 U.S.C. 103 are summarized as follows:
1. Determining the scope and contents of the prior art.
2. Ascertaining the differences between the prior art and the claims at issue.
3. Resolving the level of ordinary skill in the pertinent art.
4. Considering objective evidence present in the application indicating obviousness or nonobviousness.
Claims 50-55 and 57-73 are broadly drawn to a recombinant virus vector system comprising a tospovirus and at least a polynucleotide sequence encoding a sequence specific nucleic acid modifying enzyme that targets a genomic sequence in a plant cell, wherein the modification enzyme is a CRISPR/Cas nuclease and wherein the tospovirus is TSWV and comprises S, M and L genome segments, wherein the S and/or M segments comprise a heterologous polynucleotide sequence encoding a component of the sequence specific nucleic acid modifying enzyme, wherein the segments are in molar ratios of 1~2:2~10:1~2, plants produced from said system, a method for modifying the genetic material of a plant cell by using said system and wherein medium comprising said cells comprises ribavirin, and a kit comprising said system.
Harling et al teach methods for the targeted modification of nucleic acid regions in plant using the CRISPR/Cas system and wherein the CRISPR/Cas system is introduced using a vector system comprising single-strand RNA viruses having various advantages (see Abstract; see also col. 15 bridging col. 16; see col. 66, Examples 2, 5 and 10). Harling et al teach that TMV is a suitable virus for the claimed methodology (col. 76, ¶ 1).
Similarly, Cody et al teach multiplexed gene editing and protein overexpression using a TMV viral vector which obviates the prior art problems of using CRISPR based editing processes in plants (see Abstract; see also p. 23, col. 2, ¶ 1; see also p. 24, col. 1, ¶ through col. 2). The use of Streptococcus pyogenes CRISPR/Cas9 was known in the art (e.g., see p. 23, col. 1).
Cody et al teach that CP deletion prevents the virus from systematically moving throughout the plant while still allowing localized cell to cell movement through the movement protein (p. 24, col. 1, ¶ 1).
Still more, Ali et al teach the predictable use of a viral vector for introducing the CRISPR/Cas9 system to target a desired polynucleotide sequence (p. 1288, col. 2, ¶ 1). Ali et al teach that the virus mediated genome editing platform is not limited to vectors derived from TRV but that other RNA viruses could also be used to deliver gRNA molecule to various plant species (p. 1290, col. 2, ¶ 1).
Thus, while Harling et al and Cody et al and Ali et al teach that it was known in the art to use different viral vectors for introducing a sequence specific nucleic acid modifying enzyme to target and cleave a specific genomic sequence in a plant cell, the issue is whether one would have modified the teachings of Harling et al and Cody et al and Ali et al by using a TSWV viral vector as opposed to a TMV or TRV viral vector.
To this point, Feng et al teach that reconstitution of NSVs in plana is particularly difficult as all constructs need to be delivered in to the same plant cell, and because the RdRp of TSWV is exceptionally large making it difficult to express, and because achieving the proper ratios of all three genome segments is not easy or consistent (p. 1182, col. 1, ¶ 3; see also p. 1187, col. 2, ¶ 1).
In particular, Feng et al teach that S antigenomic, M genomic and L antigenomic DNA of TSWV wherein the M genomic DNA is codon optimized resulted in systemic infection and viral rescue with the addition of various RNAi suppressors including P19, HcPro and γb whereas the nonstructural protein of the S segment (NS) interfered with rescue of genomic RNA (see Abstract; see also p. 1184, col. 1, ¶ 2 bridging p. 1185; see also p. 1188, col. 2, last ¶). These constructs included or excluded reported genes (e.g., see p. 1186, col. 1).
Feng et al teach that the choice of plant promoter is one of the major and critical factors for the construction of a reverse genetics system for TSWV, and that the 35S/Pol II promoter may be successfully used (p. 1186, col. 2, last ¶ par.). VSRs such as P19 increased expression, and the GP of the segment may be replaced with a heterologous polynucleotide (p. 1183, col. 2, ¶ 1 and last ¶).
Feng et al teach that TSWV has a very broad host range infecting more than 1,000 plant species over 80 families (p. 1181, col. 1, ¶ 1).
Hu et al teach that the S, M and L genomic segments as claimed were known and available in the art (see Attachment A).
Fazio et al teach that is was known in the art the Ribavirin may be used to control TSWV systemic infection (e.g., see Abstract).
Therefore, prior to the effective filing date of the instant invention it would have been prima facie obvious to one of ordinary skill in the art to modify the teachings of Harling et al and Cody et al and Ali et al by instead using TSWV as a viral vector as taught by Feng et al because the viral vectors are functionally equivalent and could be used in a wide array of different plant hosts.
Applicant should note that the equivalency must be recognized in the prior art, and cannot be based on applicant’s disclosure or the mere fact that the components at issue are functional or mechanical equivalents. In re Ruff, 256 F.2d 590, 118 USPQ 340 (CCPA 1958). See MPEP 2144.06
Factors that will support a conclusion that the prior art element is an equivalent are: (1) the prior art element performs the identical function specified in the claim in substantially the same way, and produces substantially the same results as the corresponding element disclosed in the specification. Kemco Sales, Inc. v. Control Papers Co. , 208 F.3d 1352, 54 USPQ2d 1308 (Fed. Cir. 2000); or (2) a person of ordinary skill in the art would have recognized the interchangeability of the element shown in the prior art for the corresponding element disclosed in the specification. Caterpillar Inc. v. Deere & Co. , 224 F.3d 1374, 56 USPQ2d 1305 (Fed. Cir. 2000); or (3) there are insubstantial differences between the prior art element and the corresponding element disclosed in the specification. IMS Technology, Inc. v. Haas Automation, Inc., 206 F.3d 1422, 1436, 54 USPQ2d 1129, 1138 (Fed. Cir. 2000). See MPEP 2183.
Here, the conclusion that TSWV and other viral vectors are equivalent is based on the fact (1) that one appreciates that viral vectors function in the same way in order to introduce sequence specific nucleic acid modifying enzymes; (2) virus mediated genome editing platforms are not limited to vectors derived from TRV and that other RNA viruses could also be used to deliver gRNA molecule to various plant species (e.g., see Ali et al); and (3) there are insubstantial differences between the disclosure of the instant specification and that of Feng et al and Hu et al which teach all of the necessary elements in order to produce a recombinant TSWV viral vector system for introducing a nucleic acid modifying enzyme.
One would have a reasonable expectation of success in arriving at the viral vector because each of Harling et al and Cody et al and Ali et al teach the successful use of viral vector systems to introduce the CRISPR system for genome editing in plants.
One would have found it obvious, and with a reasonable expectation of success in arriving at the ratios as recited in instant claims 58 and 69 because differences in concentration or temperature will not support the patentability of subject matter encompassed by the prior art unless there is evidence indicating such concentration or temperature is critical. "[W]here the general conditions of a claim are disclosed in the prior art, it is not inventive to discover the optimum or workable ranges by routine experimentation." In re Aller, 220 F.2d 454, 456, 105 USPQ 233, 235 (CCPA 1955). See MPEP 2144.05 (II)(A).
Here, Feng et al clearly teach the general conditions needed in order to produce a recombinant viral vector system as claimed with a reasonable expectation of success (e.g., see p. 1182, col. 2, last ¶).
Finally, one would have found it obvious to, and with a reasonable expectation of success, to add ribavirin to the culture medium comprising cells having the viral vector system as set forth above to ensure that there is no TSWV present to infect cells or regenerated plants produced therefrom or to prevent spread of TSWV to cells or plants that have not been treated with the viral vector system.
Response to Arguments
Applicant traverses the rejection of the claims by providing references that detail the purported difficulties associated with using viral vectors (Applicant reply dated 17 November 2025, p. 17-18; see also p. 19, last ¶).
These arguments are not persuasive because they fail to specifically address one’s reasonable expectation of success with respect to the cited references used to arrive at the rejection of the claims.
Applicant traverses the rejection of the claims by arguing what is or is not taught by the references (Applicant reply dated 17 November 2025, p. 18-20).
This argument is not persuasive because as Applicant is aware, one cannot show nonobviousness by attacking references individually where the rejections are based on combinations of references. See In re Keller, 642 F.2d 413, 208 USPQ 871 (CCPA 1981); In re Merck & Co., 800 F.2d 1091, 231 USPQ 375 (Fed. Cir. 1986).
Here, Applicant has merely argued what is or is not taught by the references, but the claims are not rejected for being anticipated by the prior art. In other words, each reference need not disclose all the claim limitations.
In the instant matter, Applicant has failed to proffer any reason for why it would not have been prima facie obvious to one of ordinary skill in the art to modify the teachings of Harling et al and Cody and Ali in view of Feng and in further view of Fazio.
Conclusion
No claim is allowed.
Applicant's amendment necessitated the new ground(s) of rejection presented in this Office action. Accordingly, THIS ACTION IS MADE FINAL. See MPEP § 706.07(a). Applicant is reminded of the extension of time policy as set forth in 37 CFR 1.136(a).
A shortened statutory period for reply to this final action is set to expire THREE MONTHS from the mailing date of this action. In the event a first reply is filed within TWO MONTHS of the mailing date of this final action and the advisory action is not mailed until after the end of the THREE-MONTH shortened statutory period, then the shortened statutory period will expire on the date the advisory action is mailed, and any nonprovisional extension fee (37 CFR 1.17(a)) pursuant to 37 CFR 1.136(a) will be calculated from the mailing date of the advisory action. In no event, however, will the statutory period for reply expire later than SIX MONTHS from the mailing date of this final action.
Any inquiry concerning this communication or earlier communications from the examiner should be directed to JASON DEVEAU-ROSEN whose telephone number is (571)272-2828. The examiner can normally be reached 7:30am - 4pm.
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If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Bratislav Stankovic can be reached at (571)270-0305. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300.
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/JASON DEVEAU ROSEN/Primary Examiner, Art Unit 1662
ATTACHMENT A
Alignment of JF960235 from Hu et al with SEQ ID NO: 1 of the instant invention
JF960235
LOCUS JF960235 2970 bp RNA linear VRL 05-OCT-2011
DEFINITION Tomato spotted wilt virus isolate TSWV-YN segment S, complete
sequence.
ACCESSION JF960235
VERSION JF960235.1
KEYWORDS .
SOURCE Orthotospovirus tomatomaculae
ORGANISM Orthotospovirus tomatomaculae
Viruses; Riboviria; Orthornavirae; Negarnaviricota;
Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae;
Orthotospovirus.
REFERENCE 1 (bases 1 to 2970)
AUTHORS Hu,Z.Z., Feng,Z.K., Zhang,Z.J., Liu,Y.B. and Tao,X.R.
TITLE Complete genome sequence of a tomato spotted wilt virus isolate
from China and comparison to other TSWV isolates of different
geographic origin
JOURNAL Arch. Virol. 156 (10), 1905-1908 (2011)
PUBMED 21805095
REFERENCE 2 (bases 1 to 2970)
AUTHORS Tao,X., Hu,Z., Feng,Z. and Yao,M.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2011) Plant Protection, Plant Pathology, Weigang
1#, Nanking, Jiangsu 3431383, China
COMMENT GenBank Accession Numbers JF960235-JF960237 represent the complete
genome of Tomato spotted wilt virus isolate TSWV-YN.
FEATURES Location/Qualifiers
source 1..2970
/organism="Orthotospovirus tomatomaculae"
/mol_type="genomic RNA"
/isolate="TSWV-YN"
/host="tomato"
/db_xref="taxon:3052585"
/segment="S"
/geo_loc_name="China"
/collection_date="Jul-2010"
gene 89..1492
/gene="NSs"
CDS 89..1492
/gene="NSs"
/note="silencing suppessor"
/codon_start=1
/product="non-structural protein"
/protein_id="AEI70835.1"
/translation="MSSSVYESIIQTKASVWGSTASGKAVVDSYWIHELGTGSPLVQT
QLYSDSRSKSSFGYTAKVGNLPCEEEEILFQHVYIPIFDDIDFSININDSVLALSVCS
NTVNTNGVKHQGHLKVLSPAQLHSIGSTMNRSDITDRFQLQEKDIIPNDRYIEAANKG
SLSCVKEHTYKVEMCYNQALGKVNVLSPNRNVHEWLYCFKPNFNQVESNNRTVNSLAV
KSLLMSAENNIMPNSQAFVKASTDSHFKLNLWLRVPKVLKQISIQKLFKVAGDKTNKT
FYLSIACIPNHNSVETALNISVICKHQLPIRKCQAPFELSMMFSDLKEPYNIVHDPSY
PQRIVHALLETHTSFAQVLCNNLQEDVIIYTLNNYELTPGKLDLGERTLNYSEDICKR
KYFLSKTLECLPSNTQTMSYLDSIQIPSWKIDFARGEIKISPQSVSVAKSLLKLDLSG
IKKKESKISEAYASGSK"
gene complement(2043..2819)
/gene="N"
CDS complement(2043..2819)
/gene="N"
/codon_start=1
/product="coat protein"
/protein_id="AEI70836.1"
/translation="MSKVKLTKENIVALLTQGKDLEFEEDQNLVAFNFKTFCLENLDQ
IKKMSIISCLTFLKNRQSIVKVIKQSDFTFGKITIKKTSDRIGATDMTFRRLDSLIRV
RLVEETGNSENLNTIKSKIASHPLIQAYGLPLDDAKSVRLAIMLGGSLPLIASVDSFE
MISVVLAIYQDAKYKDLGIDPKKYDTREALGKVCTVLKSKAFEMNEDQVKKGKEYAAI
LSSSNPNAKGSIAMEHYSETLNKFYEMFGVKKQTKLAELA
Query Match 98.9%; Score 2934.4; Length 2970;
Best Local Similarity 99.7%;
Matches 2961; Conservative 0; Mismatches 6; Indels 3; Gaps 2;
Qy 1 AGAGCAATTGTGTCATAATTTTATTCTTAATCAAACCTCACTTAGAAAATCACAATACTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1 AGAGCAATTGTGTCATAATTTTATTCTTAATCAAACCTCACTTAGAAAATCACAATACTG 60
Qy 61 TAATAAGAACACAGTACCAATAACCATAATGTCTTCAAGTGTTTATGAGTCGATCATTCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 TAATAAGAACACAGTACCAATAACCATAATGTCTTCAAGTGTTTATGAGTCGATCATTCA 120
Qy 121 GACAAAAGCTTCAGTCTGGGGATCAACTGCATCTGGTAAAGCTGTTGTAGATTCTTACTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 GACAAAAGCTTCAGTCTGGGGATCAACTGCATCTGGTAAAGCTGTTGTAGATTCTTACTG 180
Qy 181 GATTCATGAACTTGGTACTGGTTCTCCACTAGTTCAAACCCAGTTGTATTCTGATTCAAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 GATTCATGAACTTGGTACTGGTTCTCCACTAGTTCAAACCCAGTTGTATTCTGATTCAAG 240
Qy 241 AAGCAAAAGTAGCTTTGGCTATACTGCAAAGGTGGGGAATCTTCCCTGTGAAGAAGAAGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 AAGCAAAAGTAGCTTTGGCTATACTGCAAAGGTGGGGAATCTTCCCTGTGAAGAAGAAGA 300
Qy 301 GATTCTTTCTCAGCATGTGTATATCCCTATTTTTGATGATATTGATTTTAGCATCAATAT 360
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
Db 301 GATTCTTTTTCAGCATGTGTATATCCCTATTTTTGATGATATTGATTTTAGCATCAATAT 360
Qy 361 TAATGACTCTGTTCTGGCACTATCTGTTTGCTCAAATACAGTTAATACTAACGGAGTGAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 TAATGACTCTGTTCTGGCACTATCTGTTTGCTCAAATACAGTTAATACTAACGGAGTGAA 420
Qy 421 ACATCAAGGTCATTTGAAAGTTTTGTCTCCTGCTCAGCTCCATTCTATTGGATCTACCAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 ACATCAAGGTCATTTGAAAGTTTTGTCTCCTGCTCAGCTCCATTCTATTGGATCTACCAT 480
Qy 481 GAACAGATCTGATATTACAGACCGATTCCAGCTCCAAGAAAAAGACATAATTCCCAATGA 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 GAACAGATCTGATATTACAGACCGATTCCAGCTCCAAGAAAAAGACATAATTCCCAATGA 540
Qy 541 CAGATACATTGAAGCTGCAAACAAGGGCTCTTTGTCTTGTGTTAAAGAGCATACCTATAA 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 CAGATACATTGAAGCTGCAAACAAGGGCTCTTTGTCTTGTGTTAAAGAGCATACCTATAA 600
Qy 601 GGTCGAGATGTGCTACAATCAAGCTTTGGGCAAAGTGAATGTTCTATCCCCTAACAGAAA 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 GGTCGAGATGTGCTACAATCAAGCTTTGGGCAAAGTGAATGTTCTATCCCCTAACAGAAA 660
Qy 661 TGTCCATGAATGGCTGTACTGTTTCAAGCCAAATTTCAATCAAGTTGAAAGCAACAACAG 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 TGTCCATGAATGGCTGTACTGTTTCAAGCCAAATTTCAATCAAGTTGAAAGCAACAACAG 720
Qy 721 AACTGTAAATTCTCTTGCAGTGAAATCTCTTCTCATGTCAGCAGAAAACAACATCATGCC 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 AACTGTAAATTCTCTTGCAGTGAAATCTCTTCTCATGTCAGCAGAAAACAACATCATGCC 780
Qy 781 TAACTCTCAGGCTTTTGTAAAAGCTTCCACTGATTCTCATTTCAAGCTGAACCTCTGGCT 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 781 TAACTCTCAGGCTTTTGTAAAAGCTTCCACTGATTCTCATTTCAAGCTGAACCTCTGGCT 840
Qy 841 AAGAGTTCCAAAGGTTTTGAAGCAGATTTCCATTCAGAAATTGTTCAAAGTTGCAGGAGA 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 841 AAGAGTTCCAAAGGTTTTGAAGCAGATTTCCATTCAGAAATTGTTCAAAGTTGCAGGAGA 900
Qy 901 TGAAACAAACAAAACATTTTATTTATCTATTGCTTGCATTCCAAACCATAACAGTGTTGA 960
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 901 TAAAACAAACAAAACATTTTATTTATCTATTGCTTGCATTCCAAACCATAACAGTGTTGA 960
Qy 961 GACAGCTTTAAACATTTCTGTTATTTGCAAGCATCAGCTCCCAATCCGTAAATGTCAAGC 1020
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Db 961 GACAGCTTTAAACATTTCTGTTATTTGCAAGCATCAGCTTCCAATCCGTAAATGTCAAGC 1020
Qy 1021 TCCTTTTGAATTATCAATGATGTTTTCTGATTTAAAGGAGCCTTACAACATTGTTCATGA 1080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1021 TCCTTTTGAATTATCAATGATGTTTTCTGATTTAAAGGAGCCTTACAACATTGTTCATGA 1080
Qy 1081 TCCTTCATACCCTCAGAGGATTGTTCATGCTCTGCTTGAAACTCACACATCTTTTGCACA 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1081 TCCTTCATACCCTCAGAGGATTGTTCATGCTCTGCTTGAAACTCACACATCTTTTGCACA 1140
Qy 1141 AGTTCTTTGCAACAACTTGCAAGAAGATGTGATCATCTACACTTTGAACAACTATGAGCT 1200
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1141 AGTTCTTTGCAACAACTTGCAAGAAGATGTGATCATCTACACTTTGAACAACTATGAGCT 1200
Qy 1201 AACTCCTGGAAAGTTAGATCTAGGTGAAAGAACCTTGAATTACAGTGAAGATATCTGCAA 1260
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1201 AACTCCTGGAAAGTTAGATCTAGGTGAAAGAACCTTGAATTACAGTGAAGATATCTGCAA 1260
Qy 1261 AAGGAAATATTTCCTTTCAAAAACACTTGAATGTCTTCCATCTAACACACAAACTATGTC 1320
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1261 AAGGAAATATTTCCTTTCAAAAACACTTGAATGTCTTCCATCTAACACACAAACTATGTC 1320
Qy 1321 TTACTTAGACAGTATCCAAATCCCTTCCTGGAAGATAGACTTTGCCAGGGGAGAAATTAA 1380
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1321 TTACTTAGACAGTATCCAAATCCCTTCCTGGAAGATAGACTTTGCCAGGGGAGAAATTAA 1380
Qy 1381 AATTTCTCCACAATCTGTTTCAGTTGCAAAATCTTTGTTAAAGCTTGATTTAAGCGGGAT 1440
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1381 AATTTCTCCACAATCTGTTTCAGTTGCAAAATCTTTGTTAAAGCTTGATTTAAGCGGGAT 1440
Qy 1441 CAAAAAGAAAGAATCTAAGATCTCGGAAGCATATGCTTCAGGATCAAAATAATCTTGCCG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1441 CAAAAAGAAAGAATCTAAGATCTCGGAAGCATATGCTTCAGGATCAAAATAATCTTGCCG 1500
Qy 1501 TGTCCAGCTTTTCCTAATTATGTTATGTTTATTTTCTTTCTTTACTCATAATTATTTCTC 1560
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1501 TGTCCAGCTTTTCCTAATTATGTTATGTTTATTTTCTTTCTTTACTCATAATTATTTCTC 1560
Qy 1561 TGTTTTGTCATTTCTTTTGAATTTCTCCTGTTTAATAGAAACCATAAAAATAAAAATAAA 1620
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1561 TGTTTTGTCATTTCTTTTGAATTTCTCCTGTTTAATAGAAACCATAAAAATAAAAATAAA 1620
Qy 1621 AATAAAAATAAAATCAAAATGAAACAAAAATCAAAAAATGAAACAAAATCAAACAAAAAT 1680
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1621 AATAAAAATAAAATCAAAATGAAACAAAAATCAAAAAATGAAACAAAATCAAACAAAAAT 1680
Qy 1681 CAAAAAATGAAATAAAAACAAC-AAAAAATTAAAAAACAAAAACCCAAAAAGATCCCGAA 1739
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
Db 1681 CAAAAAATGAAATAAAAACAACAAAAAAATTAAAAAACAAAAACCCAAAAAGATCCCGAA 1740
Qy 1740 AGGGACAATTTTGGCCAAATTTGGGTTTTGTTTTGTTTTTTGTTTTTTGTTTTTTGTTTT 1799
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1741 AGGGACAATTTTGGCCAAATTTGGGTTTTGTTTTGTTTTTTGTTTTTTGTTTTTTGTTTT 1800
Qy 1800 TATTTTATTTTTATTTTTATTTATTTATTTTATTTTATTTTATTTTATTTTTTC--TTAT 1857
||||||||||||||||||| |||||||||||||||||||||||||||||||||| ||||
Db 1801 TATTTTATTTTTATTTTTACTTATTTATTTTATTTTATTTTATTTTATTTTTTCTTTTAT 1860
Qy 1858 TTTTATTTTATGTTTTTTGTTGTTTTTGTTATTTTGTTTATTATTAAGCACAACACACAG 1917
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1861 TTTTATTTTATGTTTTTTGTTGTTTTTGTTATTTTGTTTATTATTAAGCACAACACACAG 1920
Qy 1918 AAAGCAAACTTTAATTAAACACACTTATTTTAAATTTAACACACTAAGCAAGCACAAGCA 1977
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1921 AAAGCAAACTTTAATTAAACACACTTATTTTAAATTTAACACACTAAGCAAGCACAAGCA 1980
Qy 1978 ATAAAGATAAAGAAAGCTTTATATATTTATAGGCTTTTTTATAATTTAACTTACAACTGC 2037
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1981 ATAAAGATAAAGAAAGCTTTATATATTTATAGGCTTTTTTATAATTTAACTTACAACTGC 2040
Qy 2038 TTTTAAGCAAGTTCTGCAAGTTTTGTCTGTTTTTTAACCCCGAACATTTCATAGAACTTG 2097
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2041 TTTTAAGCAAGTTCTGCAAGTTTTGTCTGTTTTTTAACCCCGAACATTTCATAGAACTTG 2100
Qy 2098 TTAAGAGTTTCACTGTAATGTTCCATAGCAATACTTCCTTTAGCATTAGGATTGCTGGAG 2157
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2101 TTAAGAGTTTCACTGTAATGTTCCATAGCAATACTTCCTTTAGCATTAGGATTGCTGGAG 2160
Qy 2158 CTAAGTATAGCAGCATACTCTTTCCCTTTCTTCACCTGATCTTCATTCATTTCAAATGCT 2217
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2161 CTAAGTATAGCAGCATACTCTTTCCCTTTCTTCACCTGATCTTCATTCATTTCAAATGCT 2220
Qy 2218 TTGCTTTTTAGCACAGTGCAAACTTTTCCTAAGGCTTCCCTGGTGTCATACTTCTTTGGA 2277
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2221 TTGCTTTTTAGCACAGTGCAAACTTTTCCTAAGGCTTCCCTGGTGTCATACTTCTTTGGA 2280
Qy 2278 TCGATCCCGAGGTCTTTGTATTTTGCATCCTGATATATAGCCAAGACAACACTGATCATC 2337
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2281 TCGATCCCGAGGTCTTTGTATTTTGCATCCTGATATATAGCCAAGACAACACTGATCATC 2340
Qy 2338 TCAAAGCTATCAACTGAAGCAATAAGAGGTAAGCTACCTCCCAGCATTATGGCAAGCCTC 2397
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2341 TCAAAGCTATCAACTGAAGCAATAAGAGGTAAGCTACCTCCCAGCATTATGGCAAGCCTC 2400
Qy 2398 ACAGACTTTGCATCATCAAGAGGTAATCCATAGGCTTGAATCAGAGGGTGAGAAGCAATC 2457
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2401 ACAGACTTTGCATCATCAAGAGGTAATCCATAGGCTTGAATCAGAGGGTGAGAAGCAATC 2460
Qy 2458 TTAGATTTGATAGTATTGAGATTCTCAGAATTCCCAGTTTCCTCGACAAGCCTGACCCTG 2517
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Db 2461 TTAGATTTGATAGTATTGAGGTTCTCAGAATTCCCAGTTTCCTCGACAAGCCTGACCCTG 2520
Qy 2518 ATCAAGCTATCAAGCCTTCTGAAGGTCATGTCAGTGGCTCCAATCCTGTCTGAAGTTTTC 2577
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2521 ATCAAGCTATCAAGCCTTCTGAAGGTCATGTCAGTGGCTCCAATCCTGTCTGAAGTTTTC 2580
Qy 2578 TTTATAGTGATTTTGCCAAAAGTAAAATCACTTTGCTTAATAACCTTCATTATACTCTGA 2637
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Db 2581 TTTATAGTGATTTTGCCAAAAGTAAAATCACTTTGCTTAATAACCTTCACTATACTCTGA 2640
Qy 2638 CGATTCTTCAGGAATGTCAGACATGAAATAATGCTCATCTTCTTGATCTGGTCAAGGTTT 2697
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2641 CGATTCTTCAGGAATGTCAGACATGAAATAATGCTCATCTTCTTGATCTGGTCAAGGTTT 2700
Qy 2698 TCCAGACAAAAAGTCTTGAAGTTAAATGCTACCAGATTCTGATCTTCTTCAAATTCAAGA 2757
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2701 TCCAGACAAAAAGTCTTGAAGTTAAATGCTACCAGATTCTGATCTTCTTCAAATTCAAGA 2760
Qy 2758 TCTTTGCCTTGTGTCAACAAAGCAACAATGTTTTCCTTAGTGAGCTTAACCTTAGACATG 2817
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2761 TCTTTGCCTTGTGTCAACAAAGCAACAATGTTTTCCTTAGTGAGCTTAACCTTAGACATG 2820
Qy 2818 ATGATCGTAGAAGTTGTTATATGCTTTGACCGTATGTAATTCAAGGTGCGAAAGTACAAC 2877
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2821 ATGATCGTAGAAGTTGTTATATGCTTTGACCGTATGTAATTCAAGGTGCGAAAGTACAAC 2880
Qy 2878 TCTGTATTCCGCAGTCGTTTCTTAGGGTTTTAATGTGATGATTTGTAAGACTGAGTGTTA 2937
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2881 TCTGTATTCCGCAGTCGTTTCTTAGGGTTTTAATGTGATGATTTGTAAGACTGAGTGTTA 2940
Qy 2938 AGGTTTGAATAAAATTGACACAATTGCTCT 2967
||||||||||||||||||||||||||||||
Db 2941 AGGTTTGAATAAAATTGACACAATTGCTCT 2970
Alignment of JF960236 from Hu et al with SEQ ID NO: 2 of the instant invention
JF960236
LOCUS JF960236 4773 bp RNA linear VRL 05-OCT-2011
DEFINITION Tomato spotted wilt virus isolate TSWV-YN segment M, complete
sequence.
ACCESSION JF960236
VERSION JF960236.1
KEYWORDS .
SOURCE Orthotospovirus tomatomaculae
ORGANISM Orthotospovirus tomatomaculae
Viruses; Riboviria; Orthornavirae; Negarnaviricota;
Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae;
Orthotospovirus.
REFERENCE 1 (bases 1 to 4773)
AUTHORS Hu,Z.Z., Feng,Z.K., Zhang,Z.J., Liu,Y.B. and Tao,X.R.
TITLE Complete genome sequence of a tomato spotted wilt virus isolate
from China and comparison to other TSWV isolates of different
geographic origin
JOURNAL Arch. Virol. 156 (10), 1905-1908 (2011)
PUBMED 21805095
REFERENCE 2 (bases 1 to 4773)
AUTHORS Tao,X., Hu,Z., Feng,Z. and Yao,M.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2011) Plant Protection, Plant Pathology, Weigang
1#, Nanking, Jiangsu 3431383, China
COMMENT GenBank Accession Numbers JF960235-JF960237 represent the complete
genome of Tomato spotted wilt virus isolate TSWV-YN.
FEATURES Location/Qualifiers
source 1..4773
/organism="Orthotospovirus tomatomaculae"
/mol_type="genomic RNA"
/isolate="TSWV-YN"
/host="tomato"
/db_xref="taxon:3052585"
/segment="M"
/geo_loc_name="China"
/collection_date="Jul-2010"
gene 101..1009
/gene="NSm"
CDS 101..1009
/gene="NSm"
/codon_start=1
/product="non-structural protein"
/protein_id="AEI70837.1"
/translation="MLTFFGNKGSSKSARKDEGPLVSLAKHNGNVEVSKPWSSSDEKL
ALTKAMDTSKGKILLNTEGTSSFGTYESDSITESEGYDLSARMIVDTNHHISNWKNDL
FVGNGKQNANKVIKICPTWDSRKQYMMISRIVIWVCPTIPNPTGKLVVALVDPNMPSE
KQIILKGQGTITDPICFVFYLNWSIPKMNNTPENCCQLHLMCSQEYKKGVSFGSVMYS
WTKEFCDSPRADKDKSCMVIPLNRAIRARSQAFIEACKLIIPKGNSEKQIKKQLKELS
SNLERSVEEEEEGVYDNVAQLSFDEI"
gene complement(1282..4689)
/gene="GP"
CDS complement(1282..4689)
/gene="GP"
/codon_start=1
/product="glycoprotein precursor"
/protein_id="AEI70838.1"
/translation="MRILKLLELVVKVSLFTIALSSVLLAFLIFRATDAKVEIIRGDH
PEVYDDSAENEVPTAASIQRKAILETLTNLMLESQTPGTRQIREEESTIPIFAGSTTQ
KIISVSDLPNNCLNASSLKCEIKGISTYNVYYQVENNGVIYSCVSDSAEGLEKCDNSL
NLPKRFSKVPVIPITKLDNKRHFSVGTKFFISESLTQDNYPITYNSYPTNGTVSLQTV
KLSGDCKITKSNFANPYTVSITSPEKIMGYLIKKPGENAEHKVISFSGSASITFTEEM
LDGEHNLLCGDKSAKIPKTNKRVRDCIIKYSKSIYKQTACINFSWIRLILIALLIYFP
IRWLVNKTTKPLFLWYDLIGLITYPILLLINCLWKYFPFKCSNCGNLCIITHECTKIC
ICNKSKASKEHSSECPILSKETDHDYNKHKWTSMEWFHLIVNTKLSFSLLKFVTEILI
GLVILSQMPMSMAQTTQCLSGCFYVPGCPVLVTSKFEKCPEKDQCYCNVKEDKIIESI
FGTNIVIEGPNDCIENQNCVAHPSIDNLIKCRLGCEYLDLFRNKPLYNGFSDYTGSSL
GLTSVGLYEAKRLRNGIIDSYNRTDKISGMIAGDSLDKNETSIPENILPRQSLIFDSV
VDGKYRYMAEQSLLGGGGTVFMLNDKTSEKAKKFVIYIKSVGIHYEVSEKYTTAPIQS
THTDFYSTCTGNCDTCRKNQALTGFQDFCITPTSYWGCEEAWCFAINEGATCGFCRNI
YDMDKSYRIYSVLKSTIVADVCISGILGGQCSRITEEVPYENALFQADIQADLHNDGI
TIGELIAHGPDSHIYSGNIANLNDPVKMFGHPQLTHDGVPIFTKKTLEGHDMSWDCAA
IGKKSITIKTCGYDTYRFRSGLEQISDIPISFKDFSSFFLEKSFSLGKLKIVVDLPSD
LFKVAPKRPSITSTRLNCNGCLLCGQGLSCILEFFSDLTFSTAISIDACSLSTYQLAV
KKGSNKYNITMFCSANPDKKKMTLYPEGNPDISVEVLVNNVIVEEPENIIDQNDEYAH
EEQQYNSDSSAWGFWDYIKSPFNFIASYFGSFFDTIRVILLIAFIFLVIYFCSILTTI
CKGYVKNKSYKSRSKIEDDDDSEIKAPMLMKDTMTRRRPPMDFSHLV
Query Match 98.9%; Score 4719.8; Length 4773;
Best Local Similarity 99.6%;
Matches 4753; Conservative 0; Mismatches 17; Indels 3; Gaps 2;
Qy 1 AGAGCAATCAGTGCATCAGAAATATACCTATTATACACTTTGCTAAGAATCAATCAATTA 60
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Db 1 AGAGCAATCAGTGCGTCAGAAATATACCTATTATACACTTTGCTAAGAATCAATCAATTA 60
Qy 61 CATTACACAAGCTCCTCTACCTCAGGCTGTTGAACTCAAAATGTTGACTTTTTTTGGTAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 61 CATTACACAAGCTCCTCTACCTCAGGCTGTTGAACTCAAAATGTTGACTTTTTTTGGTAA 120
Qy 121 TAAGGGGTCTTCTAAGTCTGCCAGAAAGGATGAAGGGCCTTTAGTTTCACTTGCTAAACA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 121 TAAGGGGTCTTCTAAGTCTGCCAGAAAGGATGAAGGGCCTTTAGTTTCACTTGCTAAACA 180
Qy 181 TAACGGTAATGTTGAAGTCTCAAAACCATGGTCTTCTTCTGATGAAAAGCTTGCTTTAAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 181 TAACGGTAATGTTGAAGTCTCAAAACCATGGTCTTCTTCTGATGAAAAGCTTGCTTTAAC 240
Qy 241 CAAAGCTATGGATACATCCAAAGGAAAGATACTGTTGAACACAGAGGGAACATCTTCCTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 241 CAAAGCTATGGATACATCCAAAGGAAAGATACTGTTGAACACAGAGGGAACATCTTCCTT 300
Qy 301 TGGAACATATGAATCTGATTCTATCACAGAATCAGAGGGTTATGATCTTTCTGCAAGAAT 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Db 301 TGGAACATATGAATCTGATTCTATCACAGAATCAGAGGGTTATGATCTTTCTGCGAGAAT 360
Qy 361 GATAGTAGATACAAACCACCATATCTCAAACTGGAAAAATGATCTTTTTGTCGGCAACGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 361 GATAGTAGATACAAACCACCATATCTCAAACTGGAAAAATGATCTTTTTGTCGGCAACGG 420
Qy 421 GAAGCAAAACGCTAATAAGGTCATCAAGATCTGTCCAACTTGGGACAGCAGAAAACAATA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 421 GAAGCAAAACGCTAATAAGGTCATCAAGATCTGTCCAACTTGGGACAGCAGAAAACAATA 480
Qy 481 CATGATGATTTCCAGGATTGTGATATGGGTCTGCCCCACTATACCAAACCCTACAGGGAA 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 481 CATGATGATTTCCAGGATTGTGATATGGGTCTGCCCCACTATACCAAACCCTACAGGGAA 540
Qy 541 ACTTGTGGTTGCTCTGGTCGATCCCAACATGCCATCTGAAAAGCAAATCATTCTGAAGGG 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 541 ACTTGTGGTTGCTCTGGTCGATCCCAACATGCCATCTGAAAAGCAAATCATTCTGAAGGG 600
Qy 601 TCAGGGGACAATAACTGATCCTATCTGTTTTGTTTTTTATCTGAACTGGTCTATTCCGAA 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 601 TCAGGGGACAATAACTGATCCTATCTGTTTTGTTTTTTATCTGAACTGGTCTATTCCGAA 660
Qy 661 AATGAATAACACTCCAGAAAACTGCTGTCAGCTGCACTTGATGTGCAGTCAAGAATACAA 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 661 AATGAATAACACTCCAGAAAACTGCTGTCAGCTGCACTTGATGTGCAGTCAAGAATACAA 720
Qy 721 GAAGGGGGTTTCTTTTGGTAGTGTCATGTATTCTTGGACAAAGGAGTTTTGTGATTCACC 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 721 GAAGGGGGTTTCTTTTGGTAGTGTCATGTATTCTTGGACAAAGGAGTTTTGTGATTCACC 780
Qy 781 CAGAGCTGATAAAGACAAAAGTTGCATGGTCATACCTCTAAACAGGGCTATTAGAGCTAG 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 781 CAGAGCTGATAAAGACAAAAGTTGCATGGTCATACCTCTAAACAGGGCTATTAGAGCTAG 840
Qy 841 ATCTCAAGCATTCATTGAGGCTTGCAAGCTGATAATTCCTAAAGGAAACAGTGAGAAGCA 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 841 ATCTCAAGCATTCATTGAGGCTTGCAAGCTGATAATTCCTAAAGGAAACAGTGAGAAGCA 900
Qy 901 GATTAAAAAACAGCTTAAAGAACTGAGCTCAAATCTTGAGAGATCAGTTGAAGAAGAGGA 960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 901 GATTAAAAAACAGCTTAAAGAACTGAGCTCAAATCTTGAGAGATCAGTTGAAGAAGAGGA 960
Qy 961 GGAAGGGGTTTCTGATAATGTTGCTCAGTTATCTTTTGATGAAATATAGTACTTTTAATA 1020
||||||||||| |||||||||||||||||||||||||||||| |||||||||||||||||
Db 961 GGAAGGGGTTTATGATAATGTTGCTCAGTTATCTTTTGATGAGATATAGTACTTTTAATA 1020
Qy 1021 CCACTTATTTAAGCTTAAATCTCTGTTTATTTTGCATTTTTAAATCAAAAACCAAAC-AA 1079
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Db 1021 CCACTTATTTAAGCTTAAATCTCTGTTTATTTTGCATTTTTAAATCAAAAACCAAACAAA 1080
Qy 1080 AAACAAAAATAAAAAACAAACAAAAAATCAAAAACAAAAAACAAAAACAAAATAAGGCTG 1139
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1081 AAACAAAAATAAAAAACAAACAAAAAATCAAAAACAAAAAACAAAAACAAAATAAGGCTG 1140
Qy 1140 AAAAGCCAAACTTTGGTCCGAAGACTCTTTTTGTTGTTTTTTGTTTATTTGTA--TTTTT 1197
|||||||||||||||||| |||||||||||||||||||||||||||||||||| |||||
Db 1141 AAAAGCCAAACTTTGGTCTGAAGACTCTTTTTGTTGTTTTTTGTTTATTTGTATTTTTTT 1200
Qy 1198 GTTTGTTTTTTGTTTATTTTATATATATATAATTCGATTAATGTAGGAAATAGGATCAAA 1257
||||||| |||||||||||||||||||||||||||||||||||||||| |||||||||||
Db 1201 GTTTGTTCTTTGTTTATTTTATATATATATAATTCGATTAATGTAGGAGATAGGATCAAA 1260
Qy 1258 TTTAGCTTGTGACAAGCAGCCTCAGACAAGGTGAGAGAAATCCATAGGTGGCCTTCGTCT 1317
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1261 TTTAGCTTGTGACAAGCAGCCTCAGACAAGGTGAGAGAAATCCATAGGTGGCCTTCGTCT 1320
Qy 1318 TGTCATTGTATCTTTCATTAACATAGGGGCTTTGATCTCAGAACCATCATCATCCTCTAT 1377
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Db 1321 TGTCATTGTATCTTTCATTAACATAGGGGCTTTGATCTCAGAATCATCATCATCCTCTAT 1380
Qy 1378 CTTGGATCTAGATTTATAAGATTTATTCTTGACATATCCTTTACAAATTGTTGTTAGAAT 1437
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1381 CTTGGATCTAGATTTATAAGATTTATTCTTGACATATCCTTTACAAATTGTTGTTAGAAT 1440
Qy 1438 AGAACAGAAATAAATCACAAGGAAAATGAATGCAATAAGCAATATCACTCTGATAGTATC 1497
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1441 AGAACAGAAATAAATCACAAGGAAAATGAATGCAATAAGCAATATCACTCTGATAGTATC 1500
Qy 1498 AAAAAATGAGCCAAAGTAACTTGCAATGAAATTGAATGGGCTTTTAATATAATCCCAGAA 1557
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1501 AAAAAATGAGCCAAAGTAACTTGCAATGAAATTGAATGGGCTTTTAATATAATCCCAGAA 1560
Qy 1558 GCCCCATGCTGAGGAATCAGAATTATATTGTTGTTCTTCATGAGCATACTCATCATTTTG 1617
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1561 GCCCCATGCTGAGGAATCAGAATTATATTGTTGTTCTTCATGAGCATACTCATCATTTTG 1620
Qy 1618 ATCTATTATGTTCTCCGGTTCTTCTACAATAACATTATTGACCAGAACTTCCACAGAAAT 1677
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1621 ATCTATTATGTTCTCCGGTTCTTCTACAATAACATTATTGACCAGAACTTCCACAGAAAT 1680
Qy 1678 ATCCGGATTGCCTTCTGGATACAATGTCATTTTCTTCTTATCCGGATTAGCAGAACAAAA 1737
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1681 ATCCGGATTGCCTTCTGGATACAATGTCATTTTCTTCTTATCCGGATTAGCAGAACAAAA 1740
Qy 1738 CATTGTTATTTTGTATTTGTTAGACCCTTTTTTAACAGCCAGCTGATAAGTAGATAGAGA 1797
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1741 CATTGTTATGTTGTATTTGTTAGACCCTTTTTTAACAGCCAGCTGATAAGTAGATAGAGA 1800
Qy 1798 GCAAGCATCTATAGAAATTGCAGTAGAAAATGTCAAATCTGAGAAAAATTCCAAAATGCA 1857
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1801 GCAAGCATCTATAGAAATTGCAGTAGAAAATGTCAAATCTGAGAAAAATTCCAAAATGCA 1860
Qy 1858 AGATAAACCTTGACCGCATAGAAGACAGCCATTGCAATTCAATCTTGTCGAAGTTATGGA 1917
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Db 1861 AGATAAACCTTGACCGCATAGAAGACAGCCGTTGCAATTCAATCTTGTCGAAGTTATGGA 1920
Qy 1918 AGGTCTCTTAGGAGCAACTTTAAAAAGATCAGATGGAAGATCAACGACAATCTTGAGTTT 1977
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1921 AGGTCTCTTAGGAGCAACTTTAAAAAGATCAGATGGAAGATCAACGACAATCTTGAGTTT 1980
Qy 1978 CCCTAAACTAAAAGATTTTTCCAAGAAAAAACTAGAGAAATCTTTGAAACTAATGGGAAT 2037
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1981 CCCTAAACTAAAAGATTTTTCCAAGAAAAAACTAGAGAAATCTTTGAAACTAATGGGAAT 2040
Qy 2038 ATCTGATATTTGCTCTAAACCAGATCTAAATCTGTATGTGTCATATCCGCATGTTTTAAT 2097
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2041 ATCTGATATTTGCTCTAAACCAGATCTAAATCTGTATGTGTCATATCCGCATGTTTTAAT 2100
Qy 2098 AGTGATTGATTTTTTCCCTATTGCTGCACAATCCCAAGACATGTCATCTCCTTCTAGAGT 2157
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Db 2101 AGTGATTGATTTTTTCCCTATTGCTGCACAATCCCAAGACATGTCATGTCCTTCTAGAGT 2160
Qy 2158 TTTCTTAGTAAAAATAGGCACTCCATCATGAGTCAATTGTGGATGACCAAACATTTTGAC 2217
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2161 TTTCTTAGTAAAAATAGGCACTCCATCATGAGTCAATTGTGGATGACCAAACATTTTGAC 2220
Qy 2218 AGGATCATTCAAGTTTGCAATATTTCCAGAATAAATATGGCTGTCAGGTCCATGAGCTAT 2277
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2221 AGGATCATTCAAGTTTGCAATATTTCCAGAATAAATATGGCTGTCAGGTCCATGAGCTAT 2280
Qy 2278 CAGTTCACCTATAGTGATACCATCATTGTGCAGATCTGCTTGTATATCAGCTTGAAACAA 2337
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2281 CAGTTCACCTATAGTGATACCATCATTGTGCAGATCTGCTTGTATATCAGCTTGAAACAA 2340
Qy 2338 TGCATTTTCATAAGGAACCTCTTCAGTAATCCTTGAGCATTGACCTCCTAAAATACCAGA 2397
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2341 TGCATTTTCATAAGGAACCTCTTCAGTAATCCTTGAGCATTGACCTCCTAAAATACCAGA 2400
Qy 2398 AATGCAAACATCTGCCACTATAGTTGATTTTAGCACTGAATAAATTCTATATGATTTATC 2457
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2401 AATGCAAACATCTGCCACTATAGTTGATTTTAGCACTGAATAAATTCTATATGATTTATC 2460
Qy 2458 CATGTCATAAATATTTCGACAGAACCCGCATGTAGCACCTTCATTAATTGCAAAACACCA 2517
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2461 CATGTCATAAATATTTCGACAGAACCCGCATGTAGCACCTTCATTAATTGCAAAACACCA 2520
Qy 2518 AGCTTCTTCACATCCCCAATAAGAAGTTGGTGTTATACAAAAATCTTGGAAACCTGTTAA 2577
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2521 AGCTTCTTCACATCCCCAATAAGAAGTTGGTGTTATACAAAAATCTTGGAAACCTGTTAA 2580
Qy 2578 AGCTTGATTTTTTCTGCAAGTGTCACAGTTTCCTGTACAAGTGGAATAAAAATCTGTGTG 2637
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2581 AGCTTGATTTTTTCTGCAAGTGTCACAGTTTCCTGTACAAGTGGAATAAAAATCTGTGTG 2640
Qy 2638 AGTGCTTTGGATAGGAGCTGTTGTGTATTTTTCAGACACTTCATAATGAATTCCCACACT 2697
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2641 AGTGCTTTGGATAGGAGCTGTTGTGTATTTTTCAGACACTTCATAATGAATTCCCACACT 2700
Qy 2698 TTTGATATAAATCACGAATTTTTTTGCTTTTTCTGAGGTCTTATCATTTAACATGAATAC 2757
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2701 TTTGATATAAATCACGAATTTTTTTGCTTTTTCTGAGGTCTTATCATTTAACATGAATAC 2760
Qy 2758 AGTCCCTCCTCCTCCTAAAAGAGATTGTTCTACCATATATCTATATTTCCCATCCACAAC 2817
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Db 2761 AGTCCCTCCTCCTCCTAAAAGAGATTGTTCTGCCATATATCTATATTTCCCATCCACAAC 2820
Qy 2818 AGAATCAAAGATCAATGATTGTCTGGGCAGAATGTTCTCTGGTATGCTTGTTTCATTTTT 2877
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Db 2821 AGAATCAAAGATCAATGATTGTCTAGGCAGAATGTTCTCTGGTATGCTTGTTTCATTTTT 2880
Qy 2878 GTCTAGAGAATCTCCTGCAATCATTCCGGAAATCTTGTCTGTACGGTTATAGGAATCTAT 2937
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2881 GTCTAGAGAATCTCCTGCAATCATTCCGGAAATCTTGTCTGTACGGTTATAGGAATCTAT 2940
Qy 2938 TATACCATTCCTCAATCTCTTAGCCTCATACAGACCAATCGATGTTAACCCCAAAGAGCT 2997
|||||||||||||||||||||||||||||||||||||| ||||||||| |||||||||||
Db 2941 TATACCATTCCTCAATCTCTTAGCCTCATACAGACCAACCGATGTTAAACCCAAAGAGCT 3000
Qy 2998 TCCTGTATAATCTGAAAACCCATTATACAAAGGTTTGTTGCGAAATAAATCTAAGTATTC 3057
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3001 TCCTGTATAATCTGAAAACCCATTATACAAAGGTTTGTTGCGAAATAAATCTAAGTATTC 3060
Qy 3058 GCAACCTAATCTACATTTTATAAGATTATCAATAGATGGGTGTGCAACACAATTCTGGTT 3117
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3061 GCAACCTAATCTACATTTTATAAGATTATCAATAGATGGGTGTGCAACACAATTCTGGTT 3120
Qy 3118 CTCTATGCAATCATTAGGACCTTCTATAACAATGTTAGTGCCAAAGATACTTTCTATAAT 3177
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3121 CTCTATGCAATCATTAGGACCTTCTATAACAATGTTAGTGCCAAAGATACTTTCTATAAT 3180
Qy 3178 CTTATCTTCTTTTACATTACAGTAACATTGATCTTTTTCAGGGCATTTTTCAAATTTGCT 3237
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3181 CTTATCTTCTTTTACATTACAGTAACATTGATCTTTTTCAGGGCATTTTTCAAATTTGCT 3240
Qy 3238 TGTAACCAAAACTGGACAGCCTGGAACATAAAAACATCCACTCAAACATTGAGTAGTTTG 3297
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3241 TGTAACCAAAACTGGACAGCCTGGAACATAAAAACATCCACTCAAACATTGAGTAGTTTG 3300
Qy 3298 AGCCATAGACATGGGCATTTGAGACAAAATGACCAAACCTATTAAGATTTCAGTCACAAA 3357
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3301 AGCCATAGACATGGGCATTTGAGACAAAATGACCAAACCTATTAAGATTTCAGTCACAAA 3360
Qy 3358 TTTCAGCAAACTAAAGCTTAGCTTAGTGTTCACTATTAGATGGAACCATTCCATGCTAGT 3417
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3361 TTTCAGCAAACTAAAGCTTAGCTTAGTGTTCACTATTAGATGGAACCATTCCATGCTAGT 3420
Qy 3418 CCACTTATGTTTGTTGTAGTCATGATCTGTTTCTTTGGACAGTATGGGACACTCTGAAGA 3477
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3421 CCACTTATGTTTGTTGTAGTCATGATCTGTTTCTTTGGACAGTATGGGACACTCTGAAGA 3480
Qy 3478 GTGTTCTTTTGAGGCTTTGCTTTTGTTGCAGATGCAAATTTTAGTACACTCATGTGTGAT 3537
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3481 GTGTTCTTTTGAGGCTTTGCTTTTGTTGCAGATGCAAATTTTAGTACACTCATGTGTGAT 3540
Qy 3538 TATGCACAAATTGCCACAGTTAGAACATTTGAATGGAAAATATTTCCATAAGCAATTTAT 3597
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3541 TATGCACAAATTGCCACAGTTAGAACATTTGAATGGAAAATATTTCCATAAGCAATTTAT 3600
Qy 3598 GAGCAACAAAATAGGGTATGTGATCAAGCCTATAAGATCATACCAGAGAAAGAGAGGTTT 3657
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3601 GAGCAACAAAATAGGGTATGTGATCAAGCCTATAAGATCATACCAGAGAAAGAGAGGTTT 3660
Qy 3658 AGTTGTCTTGTTTACTAACCACCGGATAGGGAAATAGATCAACAAAGCTATCAATATTAA 3717
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3661 AGTTGTCTTGTTTACTAACCACCGGATAGGGAAATAGATCAACAAAGCTATCAATATTAA 3720
Qy 3718 TCTTATCCAAGAGAAATTGATGCAGGCTGTTTGCTTATAAATGCTCTTTGAATATTTGAT 3777
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Db 3721 TCTTATCCAAGAGAAATTGATGCAGGCTGTTTGTTTATAAATGCTCTTTGAATATTTGAT 3780
Qy 3778 TATGCAATCTCTAACTCTTTTGTTTGTTTTTGGTATTTTAGCTGATTTGTCACCGCACAA 3837
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3781 TATGCAATCTCTAACTCTTTTGTTTGTTTTTGGTATTTTAGCTGATTTGTCACCGCACAA 3840
Qy 3838 GAGATTGTGCTCACCATCCAACATTTCTTCAGTAAAAGTGATGCTTGCTGATCCAGAAAA 3897
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3841 GAGATTGTGCTCACCATCCAACATTTCTTCAGTAAAAGTGATGCTTGCTGATCCAGAAAA 3900
Qy 3898 AGATATGACCTTGTGTTCCGCATTTTCACCAGGTTTTTTTATCAAATAACCCATGATCTT 3957
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3901 AGATATGACCTTGTGTTCCGCATTTTCACCAGGTTTTTTTATCAAATAACCCATGATCTT 3960
Qy 3958 CTCGGGGCTAGTGATGCTAACAGTATAGGGATTGGCAAAGTTTGATTTAGTTATTTTGCA 4017
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3961 CTCGGGGCTAGTGATGCTAACAGTATAGGGATTGGCAAAGTTTGATTTAGTTATTTTGCA 4020
Qy 4018 GTCTCCAGATAACTTTACGGTTTGTAATGATACTGTTCCATTAGTAGGGTACGAGTTATA 4077
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4021 GTCTCCAGATAACTTTACGGTTTGTAATGATACTGTTCCATTAGTAGGGTACGAGTTATA 4080
Qy 4078 AGTTATAGGATAATTATCTTGTGTCAGGCTTTCTGAAATGAAGAATTTTGTTCCAACTGA 4137
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4081 AGTTATAGGATAATTATCTTGTGTCAGGCTTTCTGAAATGAAGAATTTTGTTCCAACTGA 4140
Qy 4138 AAAATGCCTTTTGTTGTCAAGTTTGGTAATGGGAATAACCGGGACTTTGGAGAATCTCTT 4197
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4141 AAAATGCCTTTTGTTGTCAAGTTTGGTAATGGGAATAACCGGGACTTTGGAGAATCTCTT 4200
Qy 4198 TGGCAAATTTAAAGAATTGTCACATTTTTCTAACCCTTCTGCTGAATCAGAAACACAGGA 4257
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4201 TGGCAAATTTAAAGAATTGTCACATTTTTCTAACCCTTCTGCTGAATCAGAAACACAGGA 4260
Qy 4258 ATATATGACACCATTATTTTCCACTTGATAATAAACATTATAAGTGGATATCCCTTTTAT 4317
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4261 ATATATGACACCATTATTTTCCACTTGATAATAAACATTATAAGTGGATATCCCTTTTAT 4320
Qy 4318 CTCGCATTTTAATGAAGAAGCATTCAAGCAGTTGTTAGGAAGATCCGAGACAGAGATTAT 4377
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4321 CTCGCATTTTAATGAAGAAGCATTCAAGCAGTTGTTAGGAAGATCCGAGACAGAGATTAT 4380
Qy 4378 TTTTTGCGTAGTTGACCCAGCAAAAATAGGGATGGTTGATTCTTCTTCTCGTATCTGACG 4437
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4381 TTTTTGCGTAGTTGACCCAGCAAAAATAGGGATGGTTGATTCTTCTTCTCGTATCTGACG 4440
Qy 4438 GGTTCCAGGAGTCTGAGATTCTAGCATTAGACTAGTTAAAGTCTCCAAGATAGCCTTGCG 4497
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Db 4441 GGTTCCAGGAGTCTGAGATTCTAGCATTAGATTAGTTAAAGTCTCCAAGATAGCCTTGCG 4500
Qy 4498 TTGAATCGATGCAGCAGTGGGTACTTCATTCTCAGCAGAATCATCATAAACCTCAGGATG 4557
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4501 TTGAATCGATGCAGCAGTGGGTACTTCATTCTCAGCAGAATCATCATAAACCTCAGGATG 4560
Qy 4558 ATCTCCACGAATTATTTCTACTTTGGCATCTGTGGCTCTGAAGATCAAGAATGCCAACAA 4617
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4561 ATCTCCACGAATTATTTCTACTTTGGCATCTGTGGCTCTGAAGATCAAGAATGCCAACAA 4620
Qy 4618 GACAGAACTCAGGGCAATTGTGAAAAGACTCACTTTTACCACTAGTTCTAGTAGTTTTAA 4677
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4621 GACAGAACTCAGGGCAATTGTGAAAAGACTCACTTTTACCACTAGTTCTAGTAGTTTTAA 4680
Qy 4678 AATTCTCATCTTATACGTCTGCTCAGATTATAATGGTTATATGATTAATTTCAAGATGTC 4737
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4681 AATTCTCATCTTATACGTCTGCTCAGATTATAATGGTTATATGATTAATTTCAAGATGTC 4740
Qy 4738 TGGATTAAGGTTTTTGTTTGCACTGATTGCTCT 4770
|||||||||||||||||||||||||||||||||
Db 4741 TGGATTAAGGTTTTTGTTTGCACTGATTGCTCT 4773
Alignment of JF960237 from Hu et al with SEQ ID NO: 3 of the instant invention
LOCUS JF960237 8910 bp RNA linear VRL 05-OCT-2011
DEFINITION Tomato spotted wilt virus isolate TSWV-YN segment L, complete
sequence.
ACCESSION JF960237
VERSION JF960237.1
KEYWORDS .
SOURCE Orthotospovirus tomatomaculae
ORGANISM Orthotospovirus tomatomaculae
Viruses; Riboviria; Orthornavirae; Negarnaviricota;
Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae;
Orthotospovirus.
REFERENCE 1 (bases 1 to 8910)
AUTHORS Hu,Z.Z., Feng,Z.K., Zhang,Z.J., Liu,Y.B. and Tao,X.R.
TITLE Complete genome sequence of a tomato spotted wilt virus isolate
from China and comparison to other TSWV isolates of different
geographic origin
JOURNAL Arch. Virol. 156 (10), 1905-1908 (2011)
PUBMED 21805095
REFERENCE 2 (bases 1 to 8910)
AUTHORS Tao,X., Hu,Z., Feng,Z. and Yao,M.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2011) Plant Protection, Plant Pathology, Weigang
1#, Nanking, Jiangsu 3431383, China
COMMENT GenBank Accession Numbers JF960235-JF960237 represent the complete
genome of Tomato spotted wilt virus isolate TSWV-YN.
FEATURES Location/Qualifiers
source 1..8910
/organism="Orthotospovirus tomatomaculae"
/mol_type="genomic RNA"
/isolate="TSWV-YN"
/host="tomato"
/db_xref="taxon:3052585"
/segment="L"
/geo_loc_name="China"
/collection_date="Jul-2010"
gene 34..8670
/gene="RdRp"
CDS 34..8670
/gene="RdRp"
/codon_start=1
/product="RNA polymerase"
/protein_id="AEI70839.1"
/translation="MNIQKIQKLIENGTTLLLSIEDCVGSNHDLALDLHKRNSDEIPE
DVIINNNAKNYETMRELIVKITADGEGLNKGMATVDVKKLSEMVSLFEQKYLETELAR
HDIFGELISRHLRIKPKQRSEVEIEHALREYLDELNKKSCINKLSDDEFERINKEYVA
TNATPDNYVIYKESKNSELCLIIYDWKISVDARTETKQWRNTYKNIWKSFKDIKVNGK
PFLEDHPVFVSIVILKPIAGMPITVTSSRVLEKFEDSPSALHGERIKHARNAKLLNIS
HVGQIVGTTPTVVRNYYANTQKIKSEVRGILGDDFGSKDVFFSHWTSKYKERNPTEIA
YSEDIERIIDSLVTDEITKEEIIHFLFGNFCFHIETMNDQHIADEFRGYQNSCINLKI
EPKVDLADLKDHLIQKQQIWESLYGKHLEKIMLRIRGKKKKEKEIPDITTAFNQNAAE
YEEKYPNCFTNDLSETKTNFSMTWSPSFEKIELSSEVDYNNAIINKFRESFKSSSRVI
YNGPYSSINNQTNKARDITNLVRLCLTELSCDTTKMEKQELEDEIDINTGSIKVERTK
KSKEWNKQGSCLTRNKNEFCMKETGRENKTIYFKGLAVMNIGMSSKKRILKKEEIKER
ISKGLEYDTSERQADPNDDYSSIDMSSLTHMKKLIRHDNEDSLSWCERIKDSLFVLHN
GDIREEGKITSVYNNYAKNPECLYIQDSVLKTELETCKKINKLCNDLAIYHYSEDMMQ
FSKGLMVADRYMTKESFKILTTANTSMMLLAFKGDGMNTGGSGVPYIALHIVDEDMSD
QFNICYTKEIYSYFRNGSNYIYIMRPQRLNQVRLLSLFKTPSKVPVCFAQFSKKANEM
EKWLKNKDIEKVNVFSMTMTVKQILINIVFSSVMIGTVTKLSRMGIFDFMRYAGFLPL
SDYSNIKEYIRDKFDPDITNCGRYLFRNGIKKLLFRMEDLNLSTNAKPVVVDHENDII
GGITDLNIKCPITGSTLLTLEDLYNNVYLAIYMMPKSLHNHVHNLTSLLNVPAEWELK
FRKELGFNIFEDIYPKKAMFDDKDLFSINGALNVKALSDYYLGNIENVGLMRSEIENK
EDFLSPCYKISTLKSSKKCSQSNIISTDETIECLQNAKIQDIENWKGNNLAIIKGLIR
TYNEEKNRLVEFFEDNCVNSLYLVEKLKEIINSGSITVGKSVTSKFIRNNHPLTVETY
LKTKLYYRNNVTVLKSKKVSEELYDLVKQFHNMMEIDLDSVMNLGKGTEGKKHTFLQM
LEFVMSKAKNVTGSVDFLVSVFEKMQRTKTDREIYLMSMKVKMMLYFIEHTFKHVAQS
DPSEAISISGDNKIRALSTLSLDTITSYNDILNKNSKKSRLAFLSADQSKWSASDLTY
KYVLAIILNPILTTGEASLMIECILMYVKLKKVCIPTDIFLNLRKAQGTFGQNETAIG
LLTKGLTTNTYPVSMNWLQGNLNYLSSVYHSCAMKAYHKTLECYKDCDFQTRWIVHSD
DNATSLIASGEVDKMLTDFSSSSLPEMLFRSIEAHFKSFCITLNPKKSYASSSEVEFI
SERISKWSNYSSLLQTFSKLLHRIFTYKLFDDLMSLSIHVTMLLRKGCPNEVIPFAYG
AVQVQALSIYSMLPGEVNDSIRIFKKLGVSLKSNEIPTNMGGWLTSPIEPLSILGPSS
NDQIIYYNVIRDFLNKKSLEEVKDSVSSSSYLQMRFRELKGKYEKGTLEEKDKKMIFL
INLFEKASVSEDSDVLTIGMKFQTMLTQIIKLPNFINENALNKMSSYKDFSKLYPNLK
KNEDLYKSTKNLKIDEDAILEEDELYEKIASSLEMESVHDIMIKNPETILIAPLNDRD
FLLSQLFMYTSPSKRNQLSNQSTEKLALDRVLRSKARTFVDISSTVKMTYEENMEKKI
LEMLKFDLDSYCSFKTCVNLVIKDVNFSMLIPILDSAYPCESRKRDNYNFRWFQTEKW
IPVVEGSPGLVVMHAVYGSNYIENLGLKNIPLTDDSINVLTSTFGTGLIMEDVKSLVK
GKDSFETEAFSNSNECQRLVKACNYMIAAQNRLLAINTCFTRKSFPFYSKFNLGRGFI
SNTLALLSTIYSKEESYHFVSTASYKLDKTIRTVISAQQDMNLEKILDTAVYISDKLQ
SLFPTITREDIVLILQNVCLDSKPIWQSLEDKMKKINNSTASGFTVSNVILPHNSELN
TIQKQIVWMWNMGLCSHRTLDFVIRYIRRSDVRYVKTEEQDESRNYISGTMYKIGIMT
RSCYVQLIASDQDVAVSLRTPFEILNERDYLFDTYRESIEKLLQKFMFDKVNIINQTT
TDCFPRTRRCCIRMTTDNKMIVKVNATSRQIRLENVKLVVKIKYENVNSDVWDIIESQ
KSLVLRLPEVGECFSDMYKTADSETETIKTIKNRLMTSLTFIEAFGTLSQQIKEIVDD
DIRETMDEFLMNIRDTCLEGLENCKSVEEYDSYLDENGFNDTVELFENLLRTHDNFAN
EYSPLFSEIVDKAKQYTRDLEGFKEILLMLKYSLINDASGFKSYRATGMHAVELMAKK
HIEIGEFNLLGMIQLIKACETCHNNDSILNLASLRNVLSRTYATFGRRIRLNHDLDLQ
NNLMEKSYDFKTLVLPEIKLSELSREILKENGFVISGENLKMDRSDEEFEGLASFNVL
RLDEEEMYEGLIKEMKIKRKKKGSLFPANTLLLSELIKFLIGGIKGTSFDIETLLRNS
FRPDIFSTDRLGRLSSSVPALKVYATVYMEYKNVNCPLNEIADSLEGYLKLTKSKSKE
HFLSGRVKKALIQLRDEQSRTKKLEVYKDIANFLSRHPLCLSEKTLYGRYTYSDINDY
IMQTREIILSKISVLDEVVETDEDNFLLSYLRGEEDAFDEDDSDEEEDTD
Query Match 98.3%; Score 8764.2; Length 8910;
Best Local Similarity 99.4%;
Matches 8860; Conservative 0; Mismatches 48; Indels 7; Gaps 6;
Qy 1 AGAGCAATCAGGTACAACTAAAACATATAATCTCTCCACAGCCAGACTTTACAAATAAAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8910 AGAGCAATCAGGTACAACTAAAACATATAATCTCTCCACAGCCAGACTTTACAAATAAAT 8851
Qy 61 TATATAAGAATTCCCTCCAGTGAAACTATACCGGGACTCTATATTTATATACACTATAGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8850 TATATAAGAATTCCCTCCAGTGAAACTATACCGGGACTCTATATTTATATACACTATAGA 8791
Qy 121 TTTGGTTCACCAGATTGTCTTTAGGCTAAGTAGATCTAATCTAATTCTAAATAACAATAA 180
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8790 TTCGGTTCACCAGATTGTCTTTAGGCTAAGTAGATCTAATCTAATTCTAAATAACAATAA 8731
Qy 181 ATTTATATTCTAAGTTCTGTCTAATCTATTATTCATGCATTGTTAGGCATTACTTTTAAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8730 ATTTATATTCTAAGTTCTGTCTAATCTATTATTCATGCATTGTTAGGCATTACTTTTAAT 8671
Qy 241 TTAATCCGTGTCTTCTTCTTCATCAGAATCATCTTCGTCAAAGGCATCTTCTTCCCCTCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8670 TTAATCCGTGTCTTCTTCTTCATCAGAATCATCTTCGTCAAAGGCATCTTCTTCCCCTCT 8611
Qy 301 TAGATAACTAAGCAAGAAATTGTCTTCATCTGTTTCAACAACCTCATCCAACTCACTTAT 360
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Db 8610 TAGATAACTAAGCAAGAAATTGTCTTCATCTGTTTCAACAACCTCATCCAACACACTTAT 8551
Qy 361 TTTACTCAAAATAATCTCTCTTGTTTGCATGATATAATCATTGATATCAGAGTAAGTGTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8550 TTTACTCAAAATAATCTCTCTTGTTTGCATGATATAATCATTGATATCAGAGTAAGTGTA 8491
Qy 421 TCTTCCATACAATGTTTTTTCTGATAAGCATAATGGGTGCCTAGAAAGGAAATTTGCGAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8490 TCTTCCATACAATGTTTTTTCTGATAAGCATAATGGGTGCCTAGAAAGGAAATTTGCGAT 8431
Qy 481 ATCTTTATAGACCTCTAGTTTTTTAGTTCGCGATTGTTCATCTCTTAATTGTATCAAAGC 540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8430 ATCTTTATAGACCTCTAGTTTTTTAGTTCGCGATTGTTCATCTCTTAATTGTATCAAAGC 8371
Qy 541 TTTCTTAACTCTTCCAGATAAGAAATGCTCCTTAGACTTGCTTTTTGTCAGTTTTAGATA 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8370 TTTCTTAACTCTTCCAGATAAGAAATGCTCCTTAGACTTGCTTTTTGTCAGTTTTAGATA 8311
Qy 601 ACCTTCTAAGCTGTCAGCTATCTCATTTAAAGGACAATTGACATTCTTATATTCCATATA 660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8310 ACCTTCTAAGCTGTCAGCTATCTCATTTAAAGGACAATTGACATTCTTATATTCCATATA 8251
Qy 661 AACAGTTGCATAAACTTTGAGTGCAGGTACACTGGAACTTAATCTGCCCAGTCTGTCAGT 720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8250 AACAGTTGCATAAACTTTGAGTGCAGGTACACTGGAACTTAATCTGCCCAGTCTGTCAGT 8191
Qy 721 TGAAAATATGTCTGGTCTAAAACTATTCCGCAACAATGTCTCTATATCAAAACTGGTTCC 780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8190 TGAAAATATGTCTGGTCTAAAACTATTCCGCAACAATGTCTCTATATCAAAACTGGTTCC 8131
Qy 781 CTTTATCCCTCCAATCAAGAACTTTATCAACTCACTTAGTAAAAGTGTATTTGCCGGGAA 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8130 CTTTATCCCTCCAATCAAGAACTTTATCAACTCACTTAGTAAAAGTGTATTTGCCGGGAA 8071
Qy 841 TAAAGACCCTTTCTTTTTTCTTTTAATCTTCATTTCTTTGATCAAACCTTCATACATTTC 900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8070 TAAAGACCCTTTCTTTTTTCTTTTAATCTTCATTTCTTTGATCAAACCTTCATACATTTC 8011
Qy 901 TTCCTCATCTAGCCTCAACACATTAAAACTGGCAAGACCTTCAAATTCTTCATCAGACCT 960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 8010 TTCCTCATCTAGCCTCAACACATTAAAACTGGCAAGACCTTCAAATTCTTCATCAGACCT 7951
Qy 961 ATCCATTTTTAGATTCTCTCCAGATATAACAAACCCATTCTCTTTTAGTATCTCCCTTGA 1020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7950 ATCCATTTTTAGATTCTCTCCAGATATAACAAACCCATTCTCTTTTAGTATCTCCCTTGA 7891
Qy 1021 TAGTTCTGATAATTTTATTTCTGGTAAAACCAGTGTCTTGAAATCATAACTTTTTTCCAT 1080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7890 TAGTTCTGATAATTTTATTTCTGGTAAAACCAGTGTCTTGAAATCATAACTTTTTTCCAT 7831
Qy 1081 CAAGTTATTTTGCAAATCCAGATCATGATTCAATCTTATTCTTCTCCCAAATGTGGCATA 1140
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7830 CAAGTTATTTTGCAAATCCAGATCATGATTCAATCTTATTCTTCTCCCAAATGTGGCATA 7771
Qy 1141 TGTCCTGCTAAGAACATTCCTCAAACTTGCTAAGTTTAATATAGAGTCGTTGTTGTGGCA 1200
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Db 7770 TGTCCTGCTAAGAACATTCCTCAAACTTGCTAAGTTTAATATAGAGTCATTGTTGTGGCA 7711
Qy 1201 TGTTTCGCAAGCTTTAATCAGTTGGATCATTCCTAGCAAGTTGAATTCCCCTATCTCTAT 1260
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7710 TGTTTCGCAAGCTTTAATCAGTTGGATCATTCCTAGCAAGTTGAATTCCCCTATCTCTAT 7651
Qy 1261 GTGCTTTTTTGCCATTAGTTCGACAGCATGCATTCCAGTGGCTCTATAGCTTTTAAATCC 1320
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7650 GTGCTTTTTTGCCATTAGTTCGACAGCATGCATTCCAGTGGCTCTATAGCTTTTAAATCC 7591
Qy 1321 TGATGCATCATTTATCAAAGAATATTTAAGCATGAGCAGTATTTCTTTGAAACCTTCTAA 1380
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7590 TGATGCATCATTTATCAAAGAATATTTAAGCATGAGCAGTATTTCTTTGAAACCTTCTAA 7531
Qy 1381 ATCTCTAGTATACTGTTTTGCTTTGTCAACAATCTCTGAAAACAGAGGACTATACTCATT 1440
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7530 ATCTCTAGTATACTGTTTTGCTTTGTCAACAATCTCTGAAAACAGAGGACTATACTCATT 7471
Qy 1441 TTCAAAGTTGTCATGTGTTCTTAACAAGTTTTCGAATAGTTCTACTGTATCATTAAACCC 1500
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7470 TGCAAAGTTGTCATGTGTTCTTAACAAGTTTTCGAATAGTTCTACTGTATCATTAAACCC 7411
Qy 1501 ATTTTCATCAAGATAGCTATCATATTCTTCCACACTTTTGCAGTTTTCCAAACCTTCTAA 1560
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7410 ATTTTCATCAAGATAGCTATCATATTCTTCCACACTTTTGCAGTTTTCCAAACCTTCTAA 7351
Qy 1561 GCAGGTATCCCGGATGTTCATTAAGAATTCATCCATCGTTTCTCTGATATCATCATCCAC 1620
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7350 GCAGGTATCCCGGATGTTCATTAAGAATTCATCCATCGTTTCTCTGATATCATCATCCAC 7291
Qy 1621 AATCTCTTTGATCTGCTGTGATAAGTTTCCAAAGGCTTCTATGAAAGTTAAAGAAGTCAT 1680
|||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Db 7290 GATCTCTTTGATCTGCTGTGATAAGGTTCCAAAGGCTTCTATGAAAGTTAAAGAAGTCAT 7231
Qy 1681 AAGCCTGTTTTTTATGGTTTTGATTGTTTCAGTTTCAGAGTCTGCAGTTTTGTACATATC 1740
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7230 AAGCCTGTTTTTTATGGTTTTGATTGTTTCAGTTTCAGAGTCTGCAGTTTTGTACATATC 7171
Qy 1741 AGAGAAACATTCCCCTACTTCAGGGAGCCTTAAGACTAGAGATTTTTGGCTTTCTATAAT 1800
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7170 AGAGAAACATTCCCCTACTTCAGGGAGCCTTAAGACTAGAGATTTTTGGCTTTCTATAAT 7111
Qy 1801 ATCCCACACATCGGAGTTCACGTTTTCATATTTTATCTTCACAACTAATTTCACATTCTC 1860
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7110 ATCCCACACATCGGAGTTCACGTTTTCATATTTTATCTTCACAACTAATTTCACATTCTC 7051
Qy 1861 TAGTCTTATTTGTCTTGATGTGGCATTAACCTTTACAATCATCTTGTTGTCTGTGGTCAT 1920
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 7050 TAGTCTTATTTGTCTTGATGTGGCATTAACCTTTACAATCATCTTGTTGTCTGTGGTCAT 6991
Qy 1921 TCTGATGCAGGCATCTCCTGGTTCTAGGAAAACAATCTGTGGTTGTTTTGATTTTATTAT 1980
||||||||| |||||||||||||||||||||||||||||||||||||| ||||||||
Db 6990 TCTGATGCA-GCATCTCCTGGTTCTAGGAAAACAATCTGTGGTTGTTT--GATTTATTAT 6934
Qy 1981 GTTCACTTTATCAAACATAAATTTCTGCAATAATTTCTCTATACTTTCTCTGTATGTGTC 2040
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6933 GTTCACTTTATCAAACATAAATTTCTGCAATAATTTCTCTATACTTTCTCTGTATGTGTC 6874
Qy 2041 AAAAAGATAATCTCTTTCATTCAATATCTCAAATGGTGTCCTCAAAGAAACTGCTACATC 2100
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6873 AAAAAGATAATCTCTTTCATTCAATATCTCAAATGGTGTCCTCAAAGAAACTGCTACATC 6814
Qy 2101 TTGATCAGATGCTATCAATTGAACATAACAGCTTCTTGTCATGATCCCGATTTTGTACAT 2160
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6813 TTGATCAGATGCTATCAATTGAACATAACAGCTTCTTGTCATGATCCCGATTTTGTACAT 6754
Qy 2161 AGTTCCAGAGATATAATTTCCTGATTCGTCTTGTTCTTCAGTTTTCACATATCTTACATC 2220
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Db 6753 AGTTCCAGAGATATAATTTCGTGATTCGTCTTGTTCTTCAGTTTTCACATATCTTACATC 6694
Qy 2221 ACTTCTCCTAATATACCTGATAACAAAATCTAATGTTCTATGAGAACATAAACCCATGTT 2280
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6693 ACTTCTCCTAATATACCTGATAACAAAATCTAATGTTCTATGAGAACATAAACCCATGTT 6634
Qy 2281 CCACATCCAGACAATTTGTTTCTGGATTGTGTTCAATTCACTGTTATGTGACAGAATCAC 2340
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Db 6633 CCACATCCAGACAATTTGTTTCTGGATTGTGTTCAATTCACTGTTATGTGGCAGAATCAC 6574
Qy 2341 ATTTGACACTGTGAAGCCACTTGCTGTTGAATTGTTAATCTTTTTCATTTTGTCTTCTAG 2400
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Db 6573 ATTTGACACTGTGAAGCCGCTTGCTGTTGAATTGTTAATCTTTTTCATTTTGTCTTCTAG 6514
Qy 2401 ACTCTTCCATATAGGTTTGCTGTCTAGGCACACATTTTGCAATATCAAAACTATATCCTC 2460
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6513 ACTCTGCCATATAGGTTTGCTGTCTAGGCACACATTTTGCAATATCAAAACTATATCCTC 6454
Qy 2461 TCTTGTAATTGTTGGGAAAAGTGACTGCAATTTATCTGATATGTATACAGCAGTGTCCAG 2520
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6453 TCTTGTAATTGTTGGGAAAAGTGACTGCAATTTATCTGATATGTATACAGCAGTGTCCAG 6394
Qy 2521 TATTTTCTCTAAGTTCATATCTTGCTGAGCACTTATCACAGTTCTAATAGTTTTGTCTAA 2580
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6393 TATTTTCTCTAAGTTCATATCTTGCTGAGCACTTATCACAGTTCTAATAGTTTTGTCTAA 6334
Qy 2581 TTTATAACTAGCTGTAGAAACAAAATGATAGGATTCTTCTTTACTGTAGATGGTGGATAA 2640
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6333 TTTATAACTAGCTGTAGAAACAAAATGATAGGATTCTTCTTTACTGTAGATGGTGGATAA 6274
Qy 2641 GAGAGCTAATGTGTTTGAGATAAACCCTCTCCCTAGGTTGAATTTAGAATAGAAGGGGAA 2700
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6273 GAGAGCTAATGTGTTTGAGATAAACCCTCTCCCTAGGTTGAATTTAGAATAGAAGGGGAA 6214
Qy 2701 GCTTTTCCTAGTAAAGCATGTGTTAATTGCTAAAAGCCTGTTTTGTGCTGCTATCATATA 2760
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6213 GCTTTTCCTAGTAAAGCATGTGTTAATTGCTAAAAGCCTGTTTTGTGCTGCTATCATATA 6154
Qy 2761 ATTGCATGCTTTCACCAATCTTTGACATTCGTTAGAATTGCTGAAAGCCTCTGTTTCGAA 2820
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6153 ATTGCATGCTTTCACCAATCTTTGACATTCGTTAGAATTGCTGAAAGCCTCTGTTTCGAA 6094
Qy 2821 GCTGTCTTTGCCTTTAACTAAGGATTTTACATCCTCCATGATTAAACCTGTTCCAAACGT 2880
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6093 GCTGTCTTTGCCTTTAACTAAGGATTTTACATCCTCCATGATTAAACCTGTTCCAAACGT 6034
Qy 2881 GCTTGTTAAAACATTAATGCTATCGTCTGTTAGAGGGATGTTTTTTAAACCTAAATTTTC 2940
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 6033 GCTTGTTAAAACATTAATGCTATCGTCTGTTAGAGGGATGTTTTTTAAACCTAAATTTTC 5974
Qy 2941 TATATAATTTGATCCATAGACAGCATGCATTACCACTAATCCCGGAGAGCCTTCAACTAC 3000
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Db 5973 TATATAATTTGATCCATAGACAGCATGCATTACCACTAATCCCGGAGAGCCTTCAACAAC 5914
Qy 3001 AGGTATCCACTTCTCAGTCTGAAACCACCTGAAATTGTAGTTATCTCTTTTCCTAGATTC 3060
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5913 AGGTATCCACTTCTCAGTCTGAAACCACCTGAAATTGTAGTTATCTCTTTTCCTAGATTC 5854
Qy 3061 ACAAGGGTATGCAGAATCCAATATTGGGATCAGCATGCTGAAATTAACATCCTTGATCAC 3120
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Db 5853 ACAAGGGTATGCAGAATCCAATATTGGAATCAGCATGCTGAAATTAACATCCTTGATCAC 5794
Qy 3121 TAGATTTACACATGTTTTAAATGAACAATATGAATCTAAATCAAATTTTAGCATTTCCAA 3180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5793 TAGATTTACACATGTTTTAAATGAACAATATGAATCTAAATCAAATTTTAGCATTTCCAA 5734
Qy 3181 GATTTTCTTTTCCATGTTTTCTTCATAAGTCATCTTCACAGTGGAAGAAATGTCTACAAA 3240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5733 GATTTTCTTTTCCATGTTTTCTTCATAAGTCATCTTCACAGTGGAAGAAATGTCTACAAA 5674
Qy 3241 TGTTCTAGCTTTTGACCTTAATACTCTATCCAAAGCAAGTTTCTCTGTAGATTGGTTAGA 3300
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Db 5673 TGTTCTAGCTTTCGACCTTAATACTCTATCCAAAGCAAGTTTCTCTGTAGATTGGTTAGA 5614
Qy 3301 TAACTGATTCCTTTTAGAAGGGCTTGTGTACATGAACAGCTGACTAAGTAAAAAATCTCT 3360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5613 TAACTGATTCCTTTTAGAAGGGCTTGTGTACATGAACAGCTGACTAAGTAAAAAATCTCT 5554
Qy 3361 ATCATTCAATGGTGCTATCAGAATTGTTTCAGGATTTTTTATCATTATGTCATGAACAGA 3420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5553 ATCATTCAATGGTGCTATCAGAATTGTTTCAGGATTTTTTATCATTATGTCATGAACAGA 5494
Qy 3421 TTCCATTTCTAAGCTAGATGCAATCTTCTCATATAACTCATCTCCCTCTAAAATAGCATC 3480
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Db 5493 TTCCATTTCTAAGCTAGATGCAATCTTCTCATATAACTCATCTTCCTCTAAAATAGCATC 5434
Qy 3481 CTCGTCTATCTTTAAGTTCTTAGTACTTTTATATAAATCTTCATTCTTTTTCAAATTAGG 3540
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5433 CTCGTCTATCTTTAAGTTCTTAGTACTTTTATATAAATCTTCATTCTTTTTCAAATTAGG 5374
Qy 3541 GTAAAGTTTTGAAAAATCTTTATAACTTGACATCTTGTTTAAAGCATTCTCATTTATAAA 3600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5373 GTAAAGTTTTGAAAAATCTTTATAACTTGACATCTTGTTTAAAGCATTCTCATTTATAAA 5314
Qy 3601 ATTGGGCAATTTTATAATCTGAGTTAACATAGTTTGAAATTTCATCCCGATTGTTAGAAC 3660
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5313 ATTGGGCAATTTTATAATCTGAGTTAACATAGTTTGAAATTTCATCCCGATTGTTAGAAC 5254
Qy 3661 GTCTGAATCTTCAGACACTGATGCTTTCTCAAACAGATTGATAAGAAATATCATCTTTTT 3720
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5253 GTCTGAATCTTCAGACACTGATGCTTTCTCAAACAGATTGATAAGAAATATCATCTTTTT 5194
Qy 3721 ATCTTTTTCTTCCAGAGTTCCTTTTTCATACTTTCCTTTTAGCTCTCTGAATCTCATCTG 3780
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5193 ATCTTTTTCTTCCAGAGTTCCTTTTTCATACTTTCCTTTTAGCTCTCTGAATCTCATCTG 5134
Qy 3781 TAGATAGCTGGAAGAAGAGACGCTATCTTTTACTTCCTCTAAACTTTTTTTGTTCAAAAA 3840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5133 TAGATAGCTGGAAGAAGAGACGCTATCTTTTACTTCCTCTAAACTTTTTTTGTTCAAAAA 5074
Qy 3841 ATCTCTTATCACATTGTAATAGATGATTTGATCATTCGATGATGGACCTAATATAGACAA 3900
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5073 ATCTCTTATCACATTGTAATAGATGATTTGATCATTCGATGATGGACCTAATATAGACAA 5014
Qy 3901 TGGCTCTATAGGAGAAGTCAACCAGCCCCCCATGTTTGTGGGAATCTCGTTTGACTTTAA 3960
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 5013 TGGCTCTATAGGAGAAGTCAACCAGCCCCCCATGTTTGTGGGAATCTCGTTTGACTTTAA 4954
Qy 3961 ACTTACTCCAAGCTTCTTAAAAATTCTGATGCTATCATTCACTTCACCAGGAAGCATTGA 4020
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4953 ACTTACTCCAAGCTTCTTAAAAATTCTGATGCTATCATTCACTTCACCAGGAAGCATTGA 4894
Qy 4021 ATAGATGCTTAATGCTTGTACCTGTACAGCCCCATAAGCAAAAGGTATAACTTCATTAGG 4080
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4893 ATAGATGCTTAATGCTTGTACCTGTACAGCCCCATAAGCAAAAGGTATAACTTCATTAGG 4834
Qy 4081 ACAGCCTTTTCTCAGAAGCATCGTAACATGTATACTTAGTGACATTAGATCATCAAAATA 4140
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Db 4833 ACAGCCTTTTCTCAGAAGCATCGTAACATGTATACTTAGTGACATTAGATCATC-AAATA 4775
Qy 4141 ACTTATATGTGAAGATTCTGTGCAACAATTTGCTAAATGTCTGCAATAGAGAGGAATAAT 4200
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4774 ACTTATATGTGAAGATTCTGTGCAACAATTTGCTAAATGTCTGCAATAGAGAGGAATAAT 4715
Qy 4201 TGCTCCATTTAC-AATTCTTTCAGATATAAACTCTACTTCTGATGAAGAAGCATAACTCT 4259
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Db 4714 TGCTCCATTTACTAATTCTTTCAGATATAAACTCTACTTCTGATGAAGAAGCATAACTCT 4655
Qy 4260 TTTTTGGGTTCAAAGTTATGCAAAAGCTTTTGAAATGAGCTTCAATGCTTCTAAACAACA 4319
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4654 TTTTTGGGTTCAAAGTTATGCAAAAGCTTTTGAAATGAGCTTCAATGCTTCTAAACAACA 4595
Qy 4320 TTTCTGGCAGAGATGAGCTTGAAAAGTCTGTTAGCATTTTATCGACCTCTCCACTGGCTA 4379
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4594 TTTCTGGCAGAGATGAGCTTGAAAAGTCTGTTAGCATTTTATCGACCTCTCCACTGGCTA 4535
Qy 4380 TCAATGATGTCGCATTATCATCAGAGTGCACAATCCATCTAGTTTGGAAATCACAGTCTT 4439
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4534 TCAATGATGTCGCATTATCATCAGAGTGCACAATCCATCTAGTTTGGAAATCACAGTCTT 4475
Qy 4440 TGTAGCATTCTAAAGTCTTGTGATAAGCTTTCATTGCACAAGAGTGATAAACAGAAGACA 4499
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Db 4474 TGTAGCATTCTAAAGTCTTGTGATAAGCTTTCATTGCACAAGAATGATAAACAGAAGACA 4415
Qy 4500 GATAATTTAAATTGCCTTGTAACCAGTTCATGCTAACAGGGTATGTGTTTGTCGTCAAAC 4559
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4414 GATAATTTAAATTGCCTTGTAACCAGTTCATGCTAACAGGGTATGTGTTTGTCGTCAAAC 4355
Qy 4560 CTTTAGTCAAAAGTCCTATGGCAGTTTCATTTTGCCCGAAAGTTCCTTGAGCTTTTCTTA 4619
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4354 CTTTAGTCAAAAGTCCTATGGCAGTTTCATTTTGCCCGAAAGTTCCTTGAGCTTTTCTTA 4295
Qy 4620 GATTCAAAAAAATATCTGTTGGTATACAAACCTTCTTCAATTTAACATACATTAGAATAC 4679
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Db 4294 GATTCAAAAAAATATCTGTTGGTATACAAACCTTCTTCAATTTAACATACATTAAAATAC 4235
Qy 4680 ATTCTATCATTAAACTAGCTTCACCAGTAGTTAAAATTGGATTTAATATGATAGCTAAAA 4739
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4234 ATTCTATCATTAAACTAGCTTCACCAGTAGTTAAAATTGGATTTAATATGATAGCTAAAA 4175
Qy 4740 CATATTTATAGGTAAGGTCTGATGCCGACCATTTCGATTGATCAGCAGACAGGAAAGCCA 4799
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4174 CATATTTATAGGTAAGGTCTGATGCCGACCATTTCGATTGATCAGCAGACAGGAAAGCCA 4115
Qy 4800 ATCTTGACTTCTTTGAATTCTTGTTTAAAATGTCGTTGTAAGACGTGATTGTGTCCAAAG 4859
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Db 4114 ATCTTGACTTCTTTGAATTTTTGTTTAAAATGTCGTTGTAAGACGTGATTGTGTCCAAAG 4055
Qy 4860 ATAATGTAGAAAGTGCCCTTATTTTATTGTCTCCACTTATAGATATGGCTTCTGATGGAT 4919
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 4054 ATAATGTAGAAAGTGCCCTTATTTTATTGTCTCCACTTATAGATATGGCTTCTGATGGAT 3995
Qy 4920 CACTCTGCGCAACATGTTTGAATGTGTGCTCTATAAAATAAAGCATCATTTTCACTTTCA 4979
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3994 CACTCTGCGCAACATGTTTGAATGTGTGCTCTATAAAATAAAGCATCATTTTCACTTTCA 3935
Qy 4980 TACTCATCAAGTATATTTCTCTGTCTGTTTTGGTTCTCTGCATTTTTTCAAAAACAGAAA 5039
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3934 TACTCATCAAGTATATTTCTCTGTCTGTTTTGGTTCTCTGCATTTTTTCAAAAACAGAAA 3875
Qy 5040 CTAGAAAATCTACAGACCCGGTGACATTTTTAGCTTTGGACATGACAAATTCAAGCATCT 5099
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3874 CTAGAAAATCTACAGACCCGGTGACATTTTTAGCTTTGGACATGACAAATTCAAGCATCT 3815
Qy 5100 GCAAGAATGTGTGTTTTTTCCCTTCTGTACCTTTCCCAAGGTTCATAACAGAATCTAAGT 5159
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Db 3814 GCAAGAATGTGTGTTTTTTTCCTTCTGTACCTTTCCCAAGGTTCATAACAGAATCTAAGT 3755
Qy 5160 CTATTTCCATCATGTTATGAAACTGTTTTACAAGGTCATAAAGCTCCTCTGATACTTTTT 5219
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3754 CTATTTCCATCATGTTATGAAACTGTTTTACAAGGTCATAAAGCTCCTCTGATACTTTTT 3695
Qy 5220 TAGACTTTAAAACAGTCACATTATTTCTATAATATAGTTTTGTTTTGAGATATGTTTCTA 5279
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3694 TAGACTTTAAAACAGTCACATTATTTCTATAATATAGTTTTGTTTTGAGATATGTTTCTA 3635
Qy 5280 CTGTTAAAGGATGGTTATTTCTTATAAATTTAGATGTTACGGATTTCCCTACAGTTATCG 5339
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Db 3634 CTGTTAAAGGATGGTTATTTCTTATAAATTTAGATGTTACGGATTTCCCTACAGTTATTG 3575
Qy 5340 ATCCACTATTAATTATCTCTTTAAGCTTTTCTACAAGATACAGTGAATTGACACAATTAT 5399
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3574 ATCCACTATTAATTATCTCTTTAAGCTTTTCTACAAGATACAGTGAATTGACACAATTAT 3515
Qy 5400 CTTCAAAAAATTCCACCAATCTGTTTTTCTCCTCATTGTAGGTTCTTATAAGCCCTTTTA 5459
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3514 CTTCAAAAAATTCCACCAATCTGTTTTTCTCCTCATTGTAGGTTCTTATAAGCCCTTTTA 3455
Qy 5460 TAATGGCCAGGTTATTTCCTTTCCAATTTTCTATATCTTGGATCTTTGCATTCTGGAGAC 5519
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3454 TAATGGCCAGGTTATTTCCTTTCCAATTTTCTATATCTTGGATCTTTGCATTCTGGAGAC 3395
Qy 5520 ACTCTATTATCTCATCAGTACTTATAATGTTTGACTGCGAGCATTTTTTTGAAGATTTTA 5579
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3394 ACTCTATTGTCTCATCAGTACTTATAATGTTTGACTGCGAGCATTTTTTTGAAGATTTTA 3335
Qy 5580 AAGTAGATATTTTATAACAAGGGCTTAGAAAATCTTCTTTATTTTCTATTTCTGATCTCA 5639
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3334 AAGTAGATATTTTATAACAAGGGCTTAGAAAATCTTCTTTATTTTCTATTTCTGATCTCA 3275
Qy 5640 TCAAACCCACATTTTCTATATTTCCTAGATAGTAATCAGATAATGCTTTCACGTTCAAAG 5699
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3274 TCAAACCCACATTTTCTATATTTCCTAGATAGTAATCAGATAATGCTTTCACGTTCAAAG 3215
Qy 5700 CTCCATTTATAGAGAATAGGTCTTTGTCATCAAACATTGCTTTCTTAGGGTATATGTCCT 5759
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3214 CTCCATTTATAGAGAATAGGTCTTTGTCATCAAACATTGCTTTCTTAGGGTATATGTCCT 3155
Qy 5760 CAAATATGTTGAAACCTAACTCTTTTCTGAACTTTAGCTCCCACTCAGCAGGGACATTTA 5819
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3154 CAAATATGTTGAAACCTAACTCTTTTCTGAACTTTAGCTCCCACTCAGCAGGGACATTTA 3095
Qy 5820 ACAAGCTTGTTAGATTGTGAACGTGATTGTGCAATGATTTAGGCATCATGTAAATAGCCA 5879
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3094 ACAAGCTTGTTAGATTGTGAACGTGATTGTGCAATGATTTAGGCATCATGTAAATAGCCA 3035
Qy 5880 AATAGACATTATTATACAAATCCTCAAGAGTCAGTAGAGTTGATCCTGTTATTGGACATT 5939
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 3034 AATAGACATTATTATACAAATCCTCAAGAGTCAGTAGAGTTGATCCTGTTATTGGACATT 2975
Qy 5940 TTATATTCAAGTCTGTTATCCCTCCTATAATATCATTTTCATGATCTACAACAACGGGTT 5999
|||||||||||||||||||||||||||||||||||||||| |||||||||||||| || |
Db 2974 TTATATTCAAGTCTGTTATCCCTCCTATAATATCATTTTCGTGATCTACAACAACAGGCT 2915
Qy 6000 TGGCATTTGTGCTTAAATTGAGATCTTCCATTCTGAACAATAGCTTTTTGATTCCATTAA 6059
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Db 2914 TGGCATTTGTGCTTAAATTGAGATCTTCCATTCTGAACAATAGCTTTTTGATTCCATT-A 2856
Qy 6060 CGAAATAGATATCTGCCACA-TTGGTTATATCAGGATCAAATTTGTCTCTAATGTACTCT 6118
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Db 2855 CGAAATAGATATCTGCCACAGTTGGTTATATCAGGATCAAATTTGTCTCTAATGTACTCT 2796
Qy 6119 TTTATATTAGAATAATCAGACAGTGGCAAAAAACCTGCATACCGCATGAAGTCAAAAATT 6178
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2795 TTTATATTAGAATAATCAGACAGTGGCAAAAAACCTGCATACCGCATGAAGTCAAAAATT 2736
Qy 6179 CCCATCCTGCTGAGCTTTGTCACAGTTCCTATCATGACAGATGAAAACACAATATTTATT 6238
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2735 CCCATCCTGCTGAGCTTTGTCACAGTTCCTATCATGACAGATGAAAACACAATATTTATT 2676
Qy 6239 AATATCTGTTTTACAGTCATTGTCATAGAAAAGACATTTACTTTTTCTATATCTTTGTTT 6298
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2675 AATATCTGTTTTACAGTCATTGTCATAGAAAAGACATTTACTTTTTCTATATCTTTGTTT 2616
Qy 6299 TTCAGCCATTTTTCCATTTCGTTAGCTTTCTTTGAAAATTGTGCAAAACATACAGGAACT 6358
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2615 TTCAGCCATTTTTCCATTTCGTTAGCTTTCTTTGAAAATTGTGCAAAACATACAGGAACT 2556
Qy 6359 TTACTAGGCGTTTTGAAAAGGCTTAGCAGCCTCACCTGATTGAGTCTCTGTGGTCTCATT 6418
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2555 TTACTAGGCGTTTTGAAAAGGCTTAGCAGCCTCACCTGATTGAGTCTCTGTGGTCTCATT 2496
Qy 6419 ATATAAATGTAATTACTACCATTTCGGAAATAGCTATAAATTTCTTTAGTATAACATATG 6478
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2495 ATATAAATGTAATTACTACCATTTCGGAAATAGCTATAAATTTCTTTAGTATAACATATG 2436
Qy 6479 TTAAATTGATCTGACATGTCTTCATCCACTATATGCAATGCTATGTAAGGAACTCCTGAT 6538
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2435 TTAAATTGATCTGACATGTCTTCATCCACTATATGCAATGCTATGTAAGGAACTCCTGAT 2376
Qy 6539 CCTCCGGTGTTCATTCCATCTCCTTTGAATGCCAATAGCATCATGCTCGTATTTGCTGTG 6598
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2375 CCTCCGGTGTTCATTCCATCTCCTTTGAATGCCAATAGCATCATGCTCGTATTTGCTGTG 2316
Qy 6599 GTTAGTATTTTGAAACTTTCTTTGGTCATGTACCTGTCAGCCACCATTAAACCTTTGGAG 6658
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2315 GTTAGTATTTTGAAACTTTCTTTGGTCATGTACCTGTCAGCCACCATTAAACCTTTGGAG 2256
Qy 6659 AATTGCATCATGTCTTCAGAGTAATGGTAAATGGCTAGGTCATTGCATAATTTGTTTATC 6718
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2255 AATTGCATCATGTCTTCAGAGTAATGGTAAATGGCTAGGTCATTGCATAATTTGTTTATC 2196
Qy 6719 TTTTTGCAAGTCTCTAATTCAGTTTTCAGTACTGAATCTTGAATGTACAAGCATTCAGGA 6778
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2195 TTTTTGCAAGTCTCTAATTCAGTTTTCAGTACTGAATCTTGAATGTACAAGCATTCAGGA 2136
Qy 6779 TTTTTAGCATAATTATTGTAAACAGATGTGATCTTGCCTTCCTCTCTTATATCACCATTA 6838
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2135 TTTTTAGCATAATTATTGTAAACAGATGTGATCTTGCCTTCCTCTCTTATATCACCATTA 2076
Qy 6839 TGAAGAACAAACAAAGAGTCCTTAATCCTTTCACACCAGCTTAAGCTATCCTCGTTGTCA 6898
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2075 TGAAGAACAAACAAAGAGTCCTTAATCCTTTCACACCAGCTTAAGCTATCCTCGTTGTCA 2016
Qy 6899 TGTCTTATCAGTTTTTTCATATGAGTCAGAGATGACATGTCTATACTTGAATAATCATCA 6958
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 2015 TGTCTTATCAGTTTTTTCATATGAGTCAGAGATGACATGTCTATACTTGAATAATCATCA 1956
Qy 6959 TTTGGGTCAGCCTGCCTTTCAGAGGTATCATATTCCAAGCCTTTAGAAATCCTCTCTTTT 7018
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1955 TTTGGGTCAGCCTGCCTTTCAGAGGTATCATATTCCAAGCCTTTAGAAATCCTCTCTTTT 1896
Qy 7019 ATTTCTTCTTTTTTTAGAATTCTTTTTTTAGAACTCATTCCTATATTCATTACTGCTAAG 7078
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1895 ATTTCTTCTTTTTTTAGAATTCTTTTTTTAGAACTCATTCCTATATTCATTACTGCTAAG 1836
Qy 7079 CCTTTAAAATAGATAGTTTTGTTCTCCCTGCCTGTCTCTTTCATGCAAAATTCATTTTTG 7138
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1835 CCTTTAAAATAGATAGTTTTGTTCTCCCTGCCTGTCTCTTTCATGCAAAATTCATTTTTG 1776
Qy 7139 TTCCTGGTTAAACACGAACCCTGCTTATTCCATTCTTTAGATTTTTTTGTTCTCTCAACT 7198
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1775 TTCCTGGTTAAACACGAACCCTGCTTATTCCATTCTTTAGATTTTTTTGTTCTCTCAACT 1716
Qy 7199 TTAATACTCCCAGTGTTTATATCTATTTCATCCTCAAGCTCCTGCTTTTCCATTTTTGTT 7258
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1715 TTAATACTCCCAGTGTTTATATCTATTTCATCCTCAAGCTCCTGCTTTTCCATTTTTGTT 1656
Qy 7259 GTATCACAACTTAGCTCTGTTAAACACAGTCTAACTAAGTTTGTTATATCTCTTGCTTTA 7318
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1655 GTATCACAACTTAGCTCTGTTAAACACAGTCTAACTAAGTTTGTTATATCTCTTGCTTTA 1596
Qy 7319 TTTGTGTGGTTATTTATGCAACTATATGGGCTATTATAAATAACCCTTGAAGAACTTTTG 7378
||||| ||||||||||||| ||||||||||| ||||||||||||||||||||||||||||
Db 1595 TTTGTTTGGTTATTTATGCTACTATATGGGCCATTATAAATAACCCTTGAAGAACTTTTG 1536
Qy 7379 AAGCTTTCCCGAAACTTGTTTATAATTGCGTTGTTGTAATCTACCTCTGAGCTCAATTCT 7438
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1535 AAGCTTTCCCGAAACTTGTTTATAATTGCGTTGTTGTAATCTACCTCTGAGCTCAATTCT 1476
Qy 7439 ATTTTTTCAAAACTTGGGGACCAAGTCATAGAAAAGTTAGTTTTAGTTTCAGAGAGATCA 7498
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1475 ATTTTTTCAAAACTTGGGGACCAAGTCATAGAAAAGTTAGTTTTAGTTTCAGAGAGATCA 1416
Qy 7499 TTTGTAAAACAGTTTGGGTACTTTTCTTCATATTCAGCAGCATTCTGGTTAAAAGCTGTG 7558
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1415 TTTGTAAAACAGTTTGGGTACTTTTCTTCATATTCAGCAGCATTCTGGTTAAAAGCTGTG 1356
Qy 7559 GTTATGTCAGGTATTTCTTTTTCTTTTTTCTTTTTTTCTCTAATTCTAAGCATGATTTTC 7618
||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Db 1355 GTTATGTCAGGTATTTCTTTTTCTTTTTTCTTTTTCCCTCTAATTCTAAGCATGATTTTC 1296
Qy 7619 TCAAGATGTTTCCCATACAGAGATTCCCATATTTGCTGCTTCTGGATTAAGTGGTCCTTC 7678
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1295 TCAAGATGTTTCCCATACAGAGATTCCCATATTTGCTGCTTCTGGATTAAGTGGTCCTTC 1236
Qy 7679 AAATCAGCTAAATCAACTTTTGGCTCTATTTTTAAATTGATACAAGAGCTTTGGTACCCT 7738
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Db 1235 AAATCAGCTAAATCAACTTTTGGCTCTATTTTTAAATTGATACAAGAGTTTTGGTACCCT 1176
Qy 7739 TTAAATTTGTCGGCGATATGCTGGTCATTCATTGTTTCAATGTGGAAACAGAAATTTCCA 7798
|||||| ||| ||||||||||||||||||||||||||||||||||||||||||||||||
Db 1175 CTAAATTCGTCAGCGATATGCTGGTCATTCATTGTTTCAATGTGGAAACAGAAATTTCCA 1116
Qy 7799 AACAAAAAATGTATTATTTCCTCTTTAGTTATTTCATCTGTAACAAGTGAATCAATTATT 7858
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1115 AACAAAAAATGTATTATTTCCTCTTTAGTTATTTCATCTGTAACAAGTGAATCAATTATT 1056
Qy 7859 CTTTCAATATCTTCAGAATAGGCTATTTCAGTAGGATTTCTTTCTTTGTATTTGCTGGTC 7918
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 1055 CTTTCAATATCTTCAGAATAGGCTATTTCAGTAGGATTTCTTTCTTTGTATTTGCTGGTC 996
Qy 7919 CAGTGACTAAAAAACACATCTTTAGATCCAAAATCATCACCTAGTATTCCTCTGACTTCA 7978
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 995 CAGTGACTAAAAAACACATCTTTAGATCCAAAATCATCACCTAGTATTCCTCTGACTTCA 936
Qy 7979 GATTTGATCTTTTGAGTGTTTGCATAATAGTTTCTCACCACTGTGGGTGTGGTTCCAACT 8038
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 935 GATTTGATCTTTTGAGTGTTTGCATAATAGTTTCTCACCACTGTGGGTGTGGTTCCAACT 876
Qy 8039 ATTCGCCCAACATGAGAAATATTTAGCAATTTGGCATTTCTAGCATGCTTTATTCTTTCT 8098
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 875 ATTTGCCCAACATGAGAAATATTTAGCAATTTGGCATTTCTAGCATGCTTTATTCTTTCT 816
Qy 8099 CCATGCAATGCTGATGGAGAATCTTCAAATTTCTCCAAAACCCTGCTGCTAGTAACAGTG 8158
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 815 CCATGCAATGCTGATGGAGAATCTTCAAATTTCTCCAAAACCCTGCTGCTAGTAACAGTG 756
Qy 8159 ATTGGCATCCCAGCAATAGGTTTCAATATAACTATAGAAACGAAAACAGGGTGATCTTCC 8218
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 755 ATTGGCATCCCAGCAATAGGTTTCAATATAACTATAGAAACGAAAACAGGGTGATCTTCC 696
Qy 8219 AGGAATGGCTTTCCATTCACTTTTATATCTTTGAAAGATTTCCAGATATTTTTGTAGTAT 8278
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 695 AGGAATGGCTTTCCATTCACTTTTATATCTTTGAAAGATTTCCAGATATTTTTGTAGGTA 636
Qy 8279 TTCTCCATTGTTTTAGTTTCTGTCCTGGCATCGACAGATATTTTCCAATCATAAATGATT 8338
|| | | |||||||||||||||||||||||||||||||||||||||||||||||||
Db 635 TTTCTCCATTGTTTAGTTTCTGTCCTGGCATCGACAGATATTTTCCAATCATAAATGATT 576
Qy 8339 AAACAAAGCTCACTGTTTTTTGATTCTTTATATATCACATAGTTGTCAGGTGTGGCGTTA 8398
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 575 AAACAAAGCTCACTGTTTTTTGATTCTTTATATATCACATAGTTGTCAGGTGTGGCGTTA 516
Qy 8399 GTTGCCACATATTCTTTATTTATTCTCTCAAACTCATCATCAGAGAGCTTATTAATGCAG 8458
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 515 GTTGCCACATATTCTTTATTTATTCTCTCAAACTCATCATCAGAGAGCTTATTAATGCAG 456
Qy 8459 GACTTTTTGTTAAGTTCATCCAGATATTCTCTGAGCGCATGCTCTATCTCCACTTCACTT 8518
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 455 GACTTTTTGTTAAGTTCATCCAGATATTCTCTGAGCGCATGCTCTATCTCCACTTCACTT 396
Qy 8519 CTTTGTTTGGGCTTTATTCTCAGGTGCCTGGAGATCAGCTCTCCAAAAATATCATGCCTT 8578
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 395 CTTTGTTTGGGCTTTATTCTCAGGTGCCTGGAGATCAGCTCTCCAAAAATATCATGCCTT 336
Qy 8579 GCTAACTCTGTTTCTAGGTATTTTTGCTCAAATAGAGAAACCATCTCACTTAACTTCTTG 8638
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Db 335 GCTAACTCTGTTTCTAGGTATTTTTGCTCAAACAGAGAAACCATCTCACTTAACTTCTTG 276
Qy 8639 ACGTCTACAGTTGCCATCCCTTTGTTTAGTCCTTCTCCATCAGCAGTGATTTTGACAATT 8698
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 275 ACGTCTACAGTTGCCATCCCTTTGTTTAGTCCTTCTCCATCAGCAGTGATTTTGACAATT 216
Qy 8699 AACTCTCTCATCGTCTCATAATTTTTTGCATTATTATTAATAATCACATCTTCTGGGATC 8758
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Db 215 AACTCTCTCATCGTCTCATAATTTTTTGCATTATTATTAATAATCACATCTTCTGGGATC 156
Qy 8759 TCATCACTATTTCTCTTATGTAAATCCAAAGCTAGGTCGTAATTAGAACCTACACAATCC 8818
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Db 155 TCATCACTATTTCTCTTATGTAAATCCAAAGCTAGGTCGTGATTAGAACCTACACAATCC 96
Qy 8819 TCAATAGACAACAATAAAGTAGTTCCATTCTCTATTAATTTTTGTATTTTCTGGATGTTC 8878
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
Db 95 TCAATAGATAACAATAAAGTAGTTCCATTCTCTATTAATTTTTGTATTTTCTGGATGTTC 36
Qy 8879 ATGTTTGCTTAAAATCGTTGTTACCTGATTGCTCT 8913
|||||||||||||||||||||||||||||||||||
Db 35 ATGTTTGCTTAAAATCGTTGTTACCTGATTGCTCT 1