CONSTRUCTION METHOD OF BACTERIAL CELLULOSE (BC)-ENRICHED PLANT USING MULTI-GENE TANDEM AND USE THEREOF

§103 §112

DETAILED ACTION Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . Continued Examination Under 37 CFR 1.114 A request for continued examination under 37 CFR 1.114, including the fee set forth in 37 CFR 1.17(e), was filed in this application after final rejection. Since this application is eligible for continued examination under 37 CFR 1.114, and the fee set forth in 37 CFR 1.17(e) has been timely paid, the finality of the previous Office action has been withdrawn pursuant to 37 CFR 1.114. Applicant's submission filed on 7/29/2026 has been entered. Status of Claims Claims 11 & 19 are under examination on the merits. Claims 5-10 & 13-18 are withdrawn. The rejection of claims 11-12 & 19-20 under 35 U.S.C. 103 over Lu et al (2002) Acta Botanica Sinica. 44 (4): 441-445, in view of GenBank AB091060.1 and GenBank sequence X54676.1, Reddy et al (2006) J. Agric. Food Chem. 54: 8077-8081, Mozes-Koch et al (2012) Plant Physiology. 158: 1883–1892, and Kawabe et al (2003) Genes Genet. Syst. 78: 343-352 is withdrawn in light of Applicant’s amendments. Claim Objections Claim 11 & 19 are objected to because of the following informalities: Claim 11 (line 3): --and-- should be inserted after “orientation,”. Claim 19 (line 3): --the rice plant comprising-- should be inserted after “wherein”. A transformation vector, which is a nucleic acid molecule, does not produce cellulose, but a plant does. Appropriate correction is required. Claim Rejections - 35 USC § 112 Indefiniteness The following is a quotation of 35 U.S.C. 112(b): (b) CONCLUSION.—The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the inventor or a joint inventor regards as the invention. The following is a quotation of 35 U.S.C. 112 (pre-AIA ), second paragraph: The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the applicant regards as his invention. Claims 11 & 19 are rejected under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA ), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention. The term “about” in claim 11 (line 10) & claim 19 (line 4) is a relative term which renders the claim indefinite. The term “about” is not defined by the claim, the specification does not provide a standard for ascertaining the requisite degree, and one of ordinary skill in the art would not be reasonably apprised of the scope of the invention. It is indefinite how much variation around the given value of 3.81% the claims encompass, so one of ordinary skill in the art would be unable to determine where infringement occurs. Additionally, claims 11 (lines 9-10) & 19 (lines 3-4) recite “bacterial cellulose at a content of about 3.81% of total cellulose content”. It is indefinite whether this limitation requires that 3.81% of the total cellulose content is bacterial cellulose (the plain language reading) or whether the cellulose content of the plant which is bacterial cellulose is 3.81% (as in paragraph [0091] of the specification). Because these two interpretations are distinct and would encompass different potential embodiments, the claims are indefinite. Written Description The following is a quotation of the first paragraph of 35 U.S.C. 112(a): (a) IN GENERAL.—The specification shall contain a written description of the invention, and of the manner and process of making and using it, in such full, clear, concise, and exact terms as to enable any person skilled in the art to which it pertains, or with which it is most nearly connected, to make and use the same, and shall set forth the best mode contemplated by the inventor or joint inventor of carrying out the invention. The following is a quotation of the first paragraph of pre-AIA 35 U.S.C. 112: The specification shall contain a written description of the invention, and of the manner and process of making and using it, in such full, clear, concise, and exact terms as to enable any person skilled in the art to which it pertains, or with which it is most nearly connected, to make and use the same, and shall set forth the best mode contemplated by the inventor of carrying out his invention. Claims 11 & 19 are rejected under 35 U.S.C. 112(a) or 35 U.S.C. 112 (pre-AIA ), first paragraph, as failing to comply with the written description requirement. The claim(s) contains subject matter which was not described in the specification in such a way as to reasonably convey to one skilled in the relevant art that the inventor or a joint inventor, or for applications subject to pre-AIA 35 U.S.C. 112, the inventor(s), at the time the application was filed, had possession of the claimed invention. This is a NEW MATTER rejection. A. Claims 11 & 19 require a recombinant plant transformation vector comprising three complete gene expression cassettes, each comprising a codon-optimized acsAB gene, acsC gene, or acsD gene. The plant transformation vector broadly requires three expression cassettes comprising a gene selected from acsAB gene, acsC gene, or acsD gene. Under the Broadest Reasonable Interpretation, this encompasses vectors that have more than one expression cassette comprising the same gene, for example, a vector comprising three expression cassettes comprising an acsAB gene. The originally filed claims required a transformation vector which comprised all three genes. The specification describes a vector which comprises one expression cassette of each gene (figure 1), paragraph [0018]), paragraphs [0052-0058]). The original disclosure does not describe any vectors other than the vector comprising one gene each of acsAB gene, acsC gene, and acsD, such as a transformation vector comprising a multi-gene cassette comprising duplicate acs genes. Thus, the amended claims encompass a broader genus of transformation vectors than is supported by Written Description in the originally filed disclosure. The expanded scope of the claimed material constitutes New Matter. In response to this rejection, Applicant is required to specifically point to support for the concept in the original disclosure or to cancel the new matter. Applicant urges that support for the amendments can be found in paragraphs [0018], [0026-0030] and working examples 1 & 3 (Remarks, page 6, paragraph 1). This argument is unpersuasive, because paragraphs [0018] and [0026-0030] and working examples 1 & 3 all describe a plant transformation vector comprising an acsAB gene, an acsC gene, and an acsD gene whereas the amended claims require an transformation vector comprising an acsAB gene, an acsC gene, or an acsD gene, which encompasses vectors which have multiple copies of one gene and no copies of others. Only a vector comprising one copy of each gene is described in the original disclosure. B. Claim 11 (lines 9-10) recites and claim 19 (lines 3-4) requires “wherein the recombinant plant transformation vector produces bacterial cellulose at a content of about 3.81% of total cellulose content”. The initially filed claims do not recite any level of bacterial cellulose production. The instant specification describes that transgenic rice had a total cellulose content 3.81% higher than the total cellulose content of the wild-type rice and concludes that the excess total cellulose in the transgenic rice was bacterial cellulose (paragraph [0091]). The claims are indefinite whether this limitation requires that 3.81% of the total cellulose content is bacterial cellulose (the plain language reading) or whether the cellulose content of the plant which is bacterial cellulose is 3.81% (which may find support in paragraph [0091] of the specification). Regardless of the interpretation, no description found in the instant application of methods or vectors which when transformed into a rice plant produce a different level of bacterial cellulose. “About 3.81% of total cellulose” may encompass a wide range of values which do not have written description support over the full scope in the originally filed disclosure. Thus, the limitation constitutes New Matter. In response to this rejection, Applicant is required to specifically point to support for the concept in the original disclosure or to cancel the new matter. Applicant urges that support for the amendments can be found in paragraphs [0018], [0026-0030] and working examples 1 & 3 (Remarks, page 6, paragraph 1). This argument is unpersuasive, because paragraph [0018] and working example 3 describe transgenic rice plants with a BC content of 3.81%, which does not fully describe the genus encompassed by “about 3.81%”. Paragraphs [0026-0030] and working example 1 do not describe the bacterial cellulose content of plants. Claim Rejections - 35 USC § 103 In the event the determination of the status of the application as subject to AIA 35 U.S.C. 102 and 103 (or as subject to pre-AIA 35 U.S.C. 102 and 103) is incorrect, any correction of the statutory basis (i.e., changing from AIA to pre-AIA ) for the rejection will not be considered a new ground of rejection if the prior art relied upon, and the rationale supporting the rejection, would be the same under either status. The following is a quotation of 35 U.S.C. 103 which forms the basis for all obviousness rejections set forth in this Office action: A patent for a claimed invention may not be obtained, notwithstanding that the claimed invention is not identically disclosed as set forth in section 102, if the differences between the claimed invention and the prior art are such that the claimed invention as a whole would have been obvious before the effective filing date of the claimed invention to a person having ordinary skill in the art to which the claimed invention pertains. Patentability shall not be negated by the manner in which the invention was made. Claim(s) 11 & 19 are rejected under 35 U.S.C. 103 as being unpatentable over Akhtar et al (2023) Plant Science. 328. 111576 (available online 12/22/2022, hereafter Akhtar) in view of GenBank AB091060.1 (available 2/9/2010) and GenBank sequence X54676.1 (available 6/13/2006), Lu et al (2002) Acta Botanica Sinica. 44 (4): 441-445 (published 2002, hereafter Lu), Reddy et al (2006) J. Agric. Food Chem. 54: 8077-8081 (published online 9/26/2002, hereafter Reddy), Mozes-Koch et al (2012) Plant Physiology. 158: 1883–1892 (published 2/21/2012, hereafter Mozes-Koch) and Kawabe et al (2003) Genes Genet. Syst. 78: 343-352 (published 2003, hereafter Kawabe). This is a new rejection necessitated by amendment. Applicant’s remarks filed 9/27/2026 have been considered as they apply to the new rejection, but they are unpersuasive. Claims 11 & 19 are drawn to a transformation vector comprising a multi-gene expression cassette comprising three gene expression cassettes comprising SEQ ID NO: 2, 4 & 6 operably linked to a 35S promoter and NOS terminator and a method comprising transforming the vector into a rice plant. Akhtar teaches that bacterial cellulose has enhanced structural purity, crystalline architecture, and polymerization compared to plant cellulose due to the absence of non-cellulosic substances, so bacterial cellulose has many industrial applications such as food and paper (page 2, left column, paragraph 4). Akhtar teaches that genetic engineering approaches have been used to enhance cotton fiber (page 2, left column, 5-right column paragraph 1). Akhtar teaches that an acs operon comprising an acsA, acsB, acsC, and acsD gene regulates cellulose production in Gluconacetobacter xylinus (formerly Acetobacter xylinum) (page 2, left column, paragraph 4). Akhtar teaches a method for expressing transgenes of acsA and acsB in cotton, comprising codon-optimization of acsA and acsB genes for G. hirsutum, synthesis in a gene cassette in pUC57, ligation into pCAMBIA 1301 and transformation into cotton via Agrobacterium transformation (page 2, right column, paragraph 3-page 3, left column, paragraph 1) leading to improvement in cotton fiber quality with greater fiber length, strength, and fineness (page 12, left column, paragraph 1). Transgenic cotton had significantly increased total cellulose in cotton fibers (258.7 μg/g in non-transgenic control plant line compared to 301.2-316.8 μg/g cellulose contents of transgenic cotton fibers; figure 7, page 7, left column, paragraph 2-right column). Akhtar teaches that the sequence for the acsA and acsB genes, which were optimized, came from the NCBI database and Du 2016 (page 2, right column, paragraph 3). Akhtar does not teach a transformation vector comprising an acsAB gene of SEQ ID NO: 2, an acsC gene of SEQ ID NO: 4 and an acsD gene of SEQ ID NO: 6 in tandem orientation and operably linked to a 35S promoter and a nopaline synthase terminator. GenBank AB091060.1 teaches a sequence from Gluconaectobacter hansenii (Novacetimonas hansenii ATCC 2376, see SOURCE) comprising acsAB, acsC and acsD genes for cellulose synthase (see DEFINTION). The acsAB sequence (first FEATURE) has 100% sequence identity to instant SEQ ID NO: 1. See alignment below. GenBank AB091060.1 teaches that this sequence encodes an acsAB protein with 100% sequence identity to the sequence encoded by SEQ ID NO: 2. See alignment of translated nucleotide SEQ ID NO: 2 (top) vs. GenBank AB091060.1 acsAB protein (bottom) below. SEQ ID NO: 1 vs GenBank AB091060.1 Score Expect Identities Gaps Strand 8593 bits(4653) 0.0 4653/4653(100%) 0/4653(0%) Plus/Plus Query 1 ATGCCAGAGGTTCGGTCGTCAACGCAGTCAGAGTCAGGAATGTCACAGTGGATGGGGAAA 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 88 ATGCCAGAGGTTCGGTCGTCAACGCAGTCAGAGTCAGGAATGTCACAGTGGATGGGGAAA 147 Query 61 ATTCTCTCCATTCGCGGTGCTGGGCTGATTATTGGTGTTTTTGGCCTGTGTGCGCTGATT 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 148 ATTCTCTCCATTCGCGGTGCTGGGCTGATTATTGGTGTTTTTGGCCTGTGTGCGCTGATT 207 Query 121 GCGGCTACGTCCGTGACCCTGCCGCCAGAACAGCAGTTGATTGTGGCATTTGTATGTGTC 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 208 GCGGCTACGTCCGTGACCCTGCCGCCAGAACAGCAGTTGATTGTGGCATTTGTATGTGTC 267 Query 181 GTGATCTTTTTTATTGTCGGTCATAAGCCCAGCCGTCGGTCCCAGATTTTCCTTGAAGTG 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 268 GTGATCTTTTTTATTGTCGGTCATAAGCCCAGCCGTCGGTCCCAGATTTTCCTTGAAGTG 327 Query 241 CTGTCAGGGCTGGTTTCGCTGCGCTATCTGACATGGCGCCTGACGGAAACGCTTTCATTC 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 328 CTGTCAGGGCTGGTTTCGCTGCGCTATCTGACATGGCGCCTGACGGAAACGCTTTCATTC 387 Query 301 GATACATGGTTGCAGGGTCTGCTTGGGACAATGCTTCTGGTGGCGGAACTTTACGCCCTG 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 388 GATACATGGTTGCAGGGTCTGCTTGGGACAATGCTTCTGGTGGCGGAACTTTACGCCCTG 447 Query 361 ATGATGCTGTTCCTCAGCTACTTCCAGACGATCGCGCCGCTGCATCGTGCGCCTCTGCCG 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 448 ATGATGCTGTTCCTCAGCTACTTCCAGACGATCGCGCCGCTGCATCGTGCGCCTCTGCCG 507 Query 421 CTGCCGCCGAACCCTGACGAATGGCCCACGGTCGATATCTTCGTCCCGACCTATAACGAA 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 508 CTGCCGCCGAACCCTGACGAATGGCCCACGGTCGATATCTTCGTCCCGACCTATAACGAA 567 Query 481 GAACTGAGCATTGTCCGCCTGACGGTGCTGGGATCACTGGGGATTGACTGGCCACCGGAA 540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 568 GAACTGAGCATTGTCCGCCTGACGGTGCTGGGATCACTGGGGATTGACTGGCCACCGGAA 627 Query 541 AAGGTGCGGGTTCATATCCTTGATGACGGCCGTCGTCCTGAATTCGCCGCCTTTGCCGCT 600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 628 AAGGTGCGGGTTCATATCCTTGATGACGGCCGTCGTCCTGAATTCGCCGCCTTTGCCGCT 687 Query 601 GAATGTGGCGCGAATTATATCGCCCGCCCGACGAACGAACATGCAAAGGCCGGTAATCTT 660 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 688 GAATGTGGCGCGAATTATATCGCCCGCCCGACGAACGAACATGCAAAGGCCGGTAATCTT 747 Query 661 AACTATGCCATTGGTCATACCGATGGTGATTACATCCTGATCTTTGACTGCGACCACGTC 720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 748 AACTATGCCATTGGTCATACCGATGGTGATTACATCCTGATCTTTGACTGCGACCACGTC 807 Query 721 CCGACCCGCGCCTTCCTGCAGTTGACAATGGGCTGGATGGTCGAAGACCCGAAGATCGCG 780 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 808 CCGACCCGCGCCTTCCTGCAGTTGACAATGGGCTGGATGGTCGAAGACCCGAAGATCGCG 867 Query 781 CTGATGCAGACCCCGCATCACTTCTATTCCCCCGACCCGTTCCAGCGGAACCTGTCGGCT 840 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 868 CTGATGCAGACCCCGCATCACTTCTATTCCCCCGACCCGTTCCAGCGGAACCTGTCGGCT 927 Query 841 GGTTATCGTACCCCGCCCGAGGGCAACCTGTTTTATGGCGTGGTGCAGGATGGCAACGAT 900 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 928 GGTTATCGTACCCCGCCCGAGGGCAACCTGTTTTATGGCGTGGTGCAGGATGGCAACGAT 987 Query 901 TTCTGGGATGCGACCTTCTTTTGCGGGTCATGTGCAATCCTGCGTCGCACGGCGATTGAG 960 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 988 TTCTGGGATGCGACCTTCTTTTGCGGGTCATGTGCAATCCTGCGTCGCACGGCGATTGAG 1047 Query 961 CAGATCGGCGGCTTTGCGACCCAGACCGTGACCGAAGACGCGCATACCGCACTCAAGATG 1020 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1048 CAGATCGGCGGCTTTGCGACCCAGACCGTGACCGAAGACGCGCATACCGCACTCAAGATG 1107 Query 1021 CAGCGTCTGGGCTGGTCCACGGCCTATCTGCGTATCCCGCTTGCCGGTGGTCTCGCGACG 1080 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1108 CAGCGTCTGGGCTGGTCCACGGCCTATCTGCGTATCCCGCTTGCCGGTGGTCTCGCGACG 1167 Query 1081 GAACGCCTGATCCTGCATATCGGACAGCGCGTGCGCTGGGCGCGTGGGATGCTGCAGATC 1140 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1168 GAACGCCTGATCCTGCATATCGGACAGCGCGTGCGCTGGGCGCGTGGGATGCTGCAGATC 1227 Query 1141 TTCCGCATCGACAATCCTCTGTTCGGGCGTGGCCTGTCATGGGGGCAGCGGCTTTGTTAC 1200 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1228 TTCCGCATCGACAATCCTCTGTTCGGGCGTGGCCTGTCATGGGGGCAGCGGCTTTGTTAC 1287 Query 1201 CTGTCGGCCATGACGTCGTTCCTGTTCGCTGTCCCGCGCGTCATCTTCCTGAGCTCCCCG 1260 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1288 CTGTCGGCCATGACGTCGTTCCTGTTCGCTGTCCCGCGCGTCATCTTCCTGAGCTCCCCG 1347 Query 1261 CTGGCGTTCCTGTTCTTTGGGCAGAACATCATTGCCGCGTCGCCGCTCGCGCTGCTGGCC 1320 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1348 CTGGCGTTCCTGTTCTTTGGGCAGAACATCATTGCCGCGTCGCCGCTCGCGCTGCTGGCC 1407 Query 1321 TATGCCATCCCGCATATGTTCCACGCCGTCGGCACGGCGTCGAAGATCAACAAGGGCTGG 1380 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1408 TATGCCATCCCGCATATGTTCCACGCCGTCGGCACGGCGTCGAAGATCAACAAGGGCTGG 1467 Query 1381 CGCTACTCCTTCTGGAGTGAGGTCTATGAAACCACCATGGCGCTGTTCCTGGTGCGCGTG 1440 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1468 CGCTACTCCTTCTGGAGTGAGGTCTATGAAACCACCATGGCGCTGTTCCTGGTGCGCGTG 1527 Query 1441 ACGATTGTCACCCTGCTCAGCCCTTCACGTGGGAAGTTCAACGTGACGGACAAGGGCGGG 1500 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1528 ACGATTGTCACCCTGCTCAGCCCTTCACGTGGGAAGTTCAACGTGACGGACAAGGGCGGG 1587 Query 1501 TTGCTTGAAAAAGGTTATTTCGACCTTGGCGCCGTCTATCCGAACATCATCCTTGGCCTG 1560 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1588 TTGCTTGAAAAAGGTTATTTCGACCTTGGCGCCGTCTATCCGAACATCATCCTTGGCCTG 1647 Query 1561 ATCATGTTCGGCGGTCTGGCGCGTGGTGTCTATGAACTGTCTTTCGGCCATCTCGACCAG 1620 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1648 ATCATGTTCGGCGGTCTGGCGCGTGGTGTCTATGAACTGTCTTTCGGCCATCTCGACCAG 1707 Query 1621 ATCGCCGAACGTGCCTACCTGCTGAACTCCGCTTGGGCGATGCTCAGCCTCATCATCATC 1680 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1708 ATCGCCGAACGTGCCTACCTGCTGAACTCCGCTTGGGCGATGCTCAGCCTCATCATCATC 1767 Query 1681 CTTGCGGCCATCGCCGTGGGGCGTGAAACACAGCAGAAACGCAACAGTCATCGCATCCCC 1740 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1768 CTTGCGGCCATCGCCGTGGGGCGTGAAACACAGCAGAAACGCAACAGTCATCGCATCCCC 1827 Query 1741 GCAACCATCCCGGTGGAAGTGGCGAATGCCGATGGGTCCATCATCGTGACGGGCGTGACC 1800 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1828 GCAACCATCCCGGTGGAAGTGGCGAATGCCGATGGGTCCATCATCGTGACGGGCGTGACC 1887 Query 1801 GAGGACCTGTCCATGGGTGGGGCCGCGGTAAAGATGTCATGGCCTGCGAAGCTGTCGGGG 1860 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1888 GAGGACCTGTCCATGGGTGGGGCCGCGGTAAAGATGTCATGGCCTGCGAAGCTGTCGGGG 1947 Query 1861 CCGACGCCGGTTTATATCCGTACTGTCCTTGATGGAGAGGAACTGATCCTGCCCGCCAGG 1920 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1948 CCGACGCCGGTTTATATCCGTACTGTCCTTGATGGAGAGGAACTGATCCTGCCCGCCAGG 2007 Query 1921 ATCATCCGTGCTGGCAACGGGCGGGGGATCTTCATCTGGACGATTGATAACCTGCAGCAG 1980 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2008 ATCATCCGTGCTGGCAACGGGCGGGGGATCTTCATCTGGACGATTGATAACCTGCAGCAG 2067 Query 1981 GAATTCTCGGTTATCCGCCTGGTGTTCGGCCGTGCCGACGCATGGGTTGACTGGGGCAAT 2040 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2068 GAATTCTCGGTTATCCGCCTGGTGTTCGGCCGTGCCGACGCATGGGTTGACTGGGGCAAT 2127 Query 2041 TACAAGGCCGACCGCCCGCTGCTCAGCCTCATGGACATGGTTCTCAGCGTCAAGGGCCTG 2100 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2128 TACAAGGCCGACCGCCCGCTGCTCAGCCTCATGGACATGGTTCTCAGCGTCAAGGGCCTG 2187 Query 2101 TTCCGTTCAAGCGGCGACATCGTCCATCGCAGTTCCCCAACCAAGCCTTCGGCTGGCAAT 2160 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2188 TTCCGTTCAAGCGGCGACATCGTCCATCGCAGTTCCCCAACCAAGCCTTCGGCTGGCAAT 2247 Query 2161 GCCCTGTCTGACGATACGAACAACCCGTCACGCAAGGAGCGTGTGCTGAAGGGAACCGTG 2220 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2248 GCCCTGTCTGACGATACGAACAACCCGTCACGCAAGGAGCGTGTGCTGAAGGGAACCGTG 2307 Query 2221 AAAATGGTTTCGCTTCTGGCGCTGCTGACATTTGCTTCCTCGGCACAGGCGGCGTCAGCG 2280 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2308 AAAATGGTTTCGCTTCTGGCGCTGCTGACATTTGCTTCCTCGGCACAGGCGGCGTCAGCG 2367 Query 2281 CCCAGGGCCGTCGCGGCGAAGGCCCCGGCCCATCAGCCCGAAGCCTCTGACCTGCCGCCG 2340 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2368 CCCAGGGCCGTCGCGGCGAAGGCCCCGGCCCATCAGCCCGAAGCCTCTGACCTGCCGCCG 2427 Query 2341 TTGCCCGCGCTCCTGCCGGCCACCAGCGGTGCGGCGCAGGCGGGTTCGGGCGATGCCGGC 2400 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2428 TTGCCCGCGCTCCTGCCGGCCACCAGCGGTGCGGCGCAGGCGGGTTCGGGCGATGCCGGC 2487 Query 2401 GCCGATGGACCCGGCAGCCCCACGGGCCAGCCCCTGGCGGCCGACAGTGCCGATGCGCTG 2460 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2488 GCCGATGGACCCGGCAGCCCCACGGGCCAGCCCCTGGCGGCCGACAGTGCCGATGCGCTG 2547 Query 2461 GTGGAAAATGCGGAAAATACGTCCGATACGGCGACTGTCCATAATTATACCCTCAAGGAT 2520 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2548 GTGGAAAATGCGGAAAATACGTCCGATACGGCGACTGTCCATAATTATACCCTCAAGGAT 2607 Query 2521 CTTGGCGCCGCAGGGTCCATCACAATGCGTGGCCTTGCCCCGTTGCAGGGGATCGAGTTC 2580 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2608 CTTGGCGCCGCAGGGTCCATCACAATGCGTGGCCTTGCCCCGTTGCAGGGGATCGAGTTC 2667 Query 2581 GGGATTCCCTCTGACCAGCTTGTGACGTCCGCGCGCCTTGTGCTGTCGGGTTCGATGTCG 2640 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2668 GGGATTCCCTCTGACCAGCTTGTGACGTCCGCGCGCCTTGTGCTGTCGGGTTCGATGTCG 2727 Query 2641 CCCAACCTGCGTCCGGAAACCAATTCGGTCACAATGACGCTGAACGAGCAGTATATCGGC 2700 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2728 CCCAACCTGCGTCCGGAAACCAATTCGGTCACAATGACGCTGAACGAGCAGTATATCGGC 2787 Query 2701 ACGTTGCGTCCCGACCCGGCGCACCCGACATTCGGCCCCATGTCGTTCGAGATCAACCCG 2760 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2788 ACGTTGCGTCCCGACCCGGCGCACCCGACATTCGGCCCCATGTCGTTCGAGATCAACCCG 2847 Query 2761 ATCTTCTTCGTCAGCGGAAACCGTCTGAACTTCAACTTCGCCTCCGGGTCGAAGGGATGT 2820 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2848 ATCTTCTTCGTCAGCGGAAACCGTCTGAACTTCAACTTCGCCTCCGGGTCGAAGGGATGT 2907 Query 2821 TCGGACATCACGAACGATACGCTGTGGGCCACGATCTCGCAGAACTCGCAGCTTCAGATC 2880 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2908 TCGGACATCACGAACGATACGCTGTGGGCCACGATCTCGCAGAACTCGCAGCTTCAGATC 2967 Query 2881 ACGACAATCGCGCTGCCGCCGCGTCGCCTGCTGTCACGTCTGCCGCAGCCCTTCTATGAC 2940 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2968 ACGACAATCGCGCTGCCGCCGCGTCGCCTGCTGTCACGTCTGCCGCAGCCCTTCTATGAC 3027 Query 2941 AAGAACGTGCGCCAGCATGTTACGGTCCCGATGGTGCTGGCGCAGACCTATGACCCGCAG 3000 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3028 AAGAACGTGCGCCAGCATGTTACGGTCCCGATGGTGCTGGCGCAGACCTATGACCCGCAG 3087 Query 3001 ATACTCAAGTCCGCGGGGATTCTCGCTTCGTGGTTTGGCAAGCAGACGGACTTCCTCGGC 3060 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3088 ATACTCAAGTCCGCGGGGATTCTCGCTTCGTGGTTTGGCAAGCAGACGGACTTCCTCGGC 3147 Query 3061 GTGACGTTCCCGGTGTCGTCCACCATCCCACAGAGTGGCAACGCCATCCTGATCGGCGTG 3120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3148 GTGACGTTCCCGGTGTCGTCCACCATCCCACAGAGTGGCAACGCCATCCTGATCGGCGTG 3207 Query 3121 GCCGATGAACTGCCGACCAGCTTCGGGCGGCCGCAGGTCAATGGCCCGGCGGTTCTGGAA 3180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3208 GCCGATGAACTGCCGACCAGCTTCGGGCGGCCGCAGGTCAATGGCCCGGCGGTTCTGGAA 3267 Query 3181 CTGCCGAACCCGTCGGATGCAAATGCCACGATCCTGGTGGTGACGGGGCGTGACCGTGAT 3240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3268 CTGCCGAACCCGTCGGATGCAAATGCCACGATCCTGGTGGTGACGGGGCGTGACCGTGAT 3327 Query 3241 GAGGTTATTACCGCGAGCAAGGGCATCGCCTTCGCGTCTGCTCCCCTGCCGACCGACAGC 3300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3328 GAGGTTATTACCGCGAGCAAGGGCATCGCCTTCGCGTCTGCTCCCCTGCCGACCGACAGC 3387 Query 3301 CATATGGATGTCGCGCCGGTCGATATCGCCCCGCGCAAGCCCAATGACGCGCCATCTTTT 3360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3388 CATATGGATGTCGCGCCGGTCGATATCGCCCCGCGCAAGCCCAATGACGCGCCATCTTTT 3447 Query 3361 ATCGCGATGGACCATCCGGTGCGCTTTGGCGACCTTGTAACGGCAAGCAAGCTACAGGGA 3420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3448 ATCGCGATGGACCATCCGGTGCGCTTTGGCGACCTTGTAACGGCAAGCAAGCTACAGGGA 3507 Query 3421 ACCGGCTTTACGTCCGGTGTGCTGTCGGTTCCGTTCCGCATTCCGCCCGACCTTTATACG 3480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3508 ACCGGCTTTACGTCCGGTGTGCTGTCGGTTCCGTTCCGCATTCCGCCCGACCTTTATACG 3567 Query 3481 TGGCGTAACCGCCCGTACAAGATGCAGGTACGTTTCCGTTCCCCCGCGGGGGAGGCGAAG 3540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3568 TGGCGTAACCGCCCGTACAAGATGCAGGTACGTTTCCGTTCCCCCGCGGGGGAGGCGAAG 3627 Query 3541 GATGTCGAGAAGTCACGTCTCGATGTCGGGATCAACGAGGTTTACCTGCATTCCTATCCG 3600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3628 GATGTCGAGAAGTCACGTCTCGATGTCGGGATCAACGAGGTTTACCTGCATTCCTATCCG 3687 Query 3601 CTGCGCGAAACGCATGGCCTGATTGGCGCGGTTTTGCAGGGTGTCGGCCTTGCCCGCCCT 3660 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3688 CTGCGCGAAACGCATGGCCTGATTGGCGCGGTTTTGCAGGGTGTCGGCCTTGCCCGCCCT 3747 Query 3661 GCAAGCGGCATGCAGGTGCATGATCTCGACGTACCGCCGTGGACCGTGTTCGGGCAGGAT 3720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3748 GCAAGCGGCATGCAGGTGCATGATCTCGACGTACCGCCGTGGACCGTGTTCGGGCAGGAT 3807 Query 3721 CAGTTGAACTTCTACTTTGACGCGATGCCCCTTGCGCGCGGAATCTGTCAAAGTGGCGCG 3780 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3808 CAGTTGAACTTCTACTTTGACGCGATGCCCCTTGCGCGCGGAATCTGTCAAAGTGGCGCG 3867 Query 3781 GCAAACAATGCATTCCATCTTGGGCTTGACCCGGATTCCACCATTGATTTTTCCCGTGCC 3840 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3868 GCAAACAATGCATTCCATCTTGGGCTTGACCCGGATTCCACCATTGATTTTTCCCGTGCC 3927 Query 3841 CATCACATCGCCCAGATGCCCAACCTTGCCTATATGGCGACGGTCGGTTTTCCTTTCACC 3900 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3928 CATCACATCGCCCAGATGCCCAACCTTGCCTATATGGCGACGGTCGGTTTTCCTTTCACC 3987 Query 3901 ACCTATGCCGATCTGTCGCAGACGGCGGTTGTTCTGCCTGAACACCCGAATGCCGCGACT 3960 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3988 ACCTATGCCGATCTGTCGCAGACGGCGGTTGTTCTGCCTGAACACCCGAATGCCGCGACT 4047 Query 3961 GTTGGCGCCTATCTTGACCTGATGGGGTTCATGGGCGCGGCGACGTGGTATCCGGTTGCG 4020 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4048 GTTGGCGCCTATCTTGACCTGATGGGGTTCATGGGCGCGGCGACGTGGTATCCGGTTGCG 4107 Query 4021 GGCGTGGACATTGTGTCGGCCGATCATGTCAGTGACGTTGCGGACCGTAACCTGCTGGTG 4080 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4108 GGCGTGGACATTGTGTCGGCCGATCATGTCAGTGACGTTGCGGACCGTAACCTGCTGGTG 4167 Query 4081 ATTTCGACGCTGGCGACAAGTGGCGAGATCGCGCCGCTGCTGTCACGTTCATCCTACGAA 4140 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4168 ATTTCGACGCTGGCGACAAGTGGCGAGATCGCGCCGCTGCTGTCACGTTCATCCTACGAA 4227 Query 4141 GTGGCGGATGGTCATTTGCGCACGGTGTCGCATGCGTCCGCGCTGGATAACGCGATCAAG 4200 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4228 GTGGCGGATGGTCATTTGCGCACGGTGTCGCATGCGTCCGCGCTGGATAACGCGATCAAG 4287 Query 4201 GCGGTCGACGATCCGCTGACGGCTTTCCGCGACCGTGACAGCAAGCCGCAGGATGTCGAT 4260 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4288 GCGGTCGACGATCCGCTGACGGCTTTCCGCGACCGTGACAGCAAGCCGCAGGATGTCGAT 4347 Query 4261 ACCCCCCTGACGGGTGGTGTCGGCGCGATGATCGAGGCGGAATCCCCCCTGACGGCGGGC 4320 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4348 ACCCCCCTGACGGGTGGTGTCGGCGCGATGATCGAGGCGGAATCCCCCCTGACGGCGGGC 4407 Query 4321 CGCACCGTTCTGGCGCTGCTGTCGTCTGACGGTGCGGGGCTGAACAACCTCCTTCAGATG 4380 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4408 CGCACCGTTCTGGCGCTGCTGTCGTCTGACGGTGCGGGGCTGAACAACCTCCTTCAGATG 4467 Query 4381 TTGGGGGAGCGCAAGAAGCAGGCGAATATCCAGGGGGACCTGGTTGTTGCCCATGGCGAG 4440 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4468 TTGGGGGAGCGCAAGAAGCAGGCGAATATCCAGGGGGACCTGGTTGTTGCCCATGGCGAG 4527 Query 4441 GACCTGTCTTCGTACCGCACTTCGCCGGTCTATACGATCGGCACCCTGCCGCTCTGGCTG 4500 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4528 GACCTGTCTTCGTACCGCACTTCGCCGGTCTATACGATCGGCACCCTGCCGCTCTGGCTG 4587 Query 4501 TGGCCGGACTGGTATATGCACAACAGACCGGTTCGTGTGCTGCTTGTCGGCCTGTTGGGA 4560 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4588 TGGCCGGACTGGTATATGCACAACAGACCGGTTCGTGTGCTGCTTGTCGGCCTGTTGGGA 4647 Query 4561 TGTATTCTGATCGTCAGTGTTCTTGCACGGGCTCTGGCGCGGCATGCGGCCCGGCGTTTC 4620 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4648 TGTATTCTGATCGTCAGTGTTCTTGCACGGGCTCTGGCGCGGCATGCGGCCCGGCGTTTC 4707 Query 4621 AAGCAGCTTGAGGATGAGAGGCGCAAGTCGTGA 4653 ||||||||||||||||||||||||||||||||| Sbjct 4708 AAGCAGCTTGAGGATGAGAGGCGCAAGTCGTGA 4740 Translated SEQ ID NO: 2 vs GenBank AB091060.1 Score Expect Method Identities Positives Gaps Frame 2809 bits(7282) 0.0 Compositional matrix adjust. 1550/1550(100%) 1550/1550(100%) 0/1550(0%) +1 Query 1 MPEVRSSTQSESGMSQWMGKILSIRGAGLIIGVFGLCALIAATSVTLPPEQQLivafvcv 180 MPEVRSSTQSESGMSQWMGKILSIRGAGLIIGVFGLCALIAATSVTLPPEQQLIVAFVCV Sbjct 1 MPEVRSSTQSESGMSQWMGKILSIRGAGLIIGVFGLCALIAATSVTLPPEQQLIVAFVCV 60 Query 181 viffivGHKPSRRSQIFLEVLSGLVSLRYLTWRLTETLSFDTWLQGLLGTMLLVAELYAL 360 VIFFIVGHKPSRRSQIFLEVLSGLVSLRYLTWRLTETLSFDTWLQGLLGTMLLVAELYAL Sbjct 61 VIFFIVGHKPSRRSQIFLEVLSGLVSLRYLTWRLTETLSFDTWLQGLLGTMLLVAELYAL 120 Query 361 MMLFLSYFQTIAplhraplplppnpdewpTVDIFVPTYNEELSIVRLTVLGSLGIDWPPE 540 MMLFLSYFQTIAPLHRAPLPLPPNPDEWPTVDIFVPTYNEELSIVRLTVLGSLGIDWPPE Sbjct 121 MMLFLSYFQTIAPLHRAPLPLPPNPDEWPTVDIFVPTYNEELSIVRLTVLGSLGIDWPPE 180 Query 541 KVRVHILDDGRRPEFAAFAAECGANYIARPTNEHAKAGNLNYAIGHTDGDYILIFDCDHV 720 KVRVHILDDGRRPEFAAFAAECGANYIARPTNEHAKAGNLNYAIGHTDGDYILIFDCDHV Sbjct 181 KVRVHILDDGRRPEFAAFAAECGANYIARPTNEHAKAGNLNYAIGHTDGDYILIFDCDHV 240 Query 721 PTRAFLQLTMGWMVEDPKIALMQTPHHFYSPDPFQRNLSAGYRTPPEGNLFYGVVQDGND 900 PTRAFLQLTMGWMVEDPKIALMQTPHHFYSPDPFQRNLSAGYRTPPEGNLFYGVVQDGND Sbjct 241 PTRAFLQLTMGWMVEDPKIALMQTPHHFYSPDPFQRNLSAGYRTPPEGNLFYGVVQDGND 300 Query 901 FWDATFFCGSCAILRRTAIEQIGGFATQTVTEDAHTALKMQRLGWSTAYLRIPLAGGLAT 1080 FWDATFFCGSCAILRRTAIEQIGGFATQTVTEDAHTALKMQRLGWSTAYLRIPLAGGLAT Sbjct 301 FWDATFFCGSCAILRRTAIEQIGGFATQTVTEDAHTALKMQRLGWSTAYLRIPLAGGLAT 360 Query 1081 ERLILHIGQRVRWARGMLQIFRIDNPLFGRGLSWGQRLCYLSAMTSFLFAVPRVIFLSSP 1260 ERLILHIGQRVRWARGMLQIFRIDNPLFGRGLSWGQRLCYLSAMTSFLFAVPRVIFLSSP Sbjct 361 ERLILHIGQRVRWARGMLQIFRIDNPLFGRGLSWGQRLCYLSAMTSFLFAVPRVIFLSSP 420 Query 1261 LAFLFFGQNIIAASPLALLAYAIPHMFHAVGTASKINKGWRYSFWSEVYETTMALFLVRV 1440 LAFLFFGQNIIAASPLALLAYAIPHMFHAVGTASKINKGWRYSFWSEVYETTMALFLVRV Sbjct 421 LAFLFFGQNIIAASPLALLAYAIPHMFHAVGTASKINKGWRYSFWSEVYETTMALFLVRV 480 Query 1441 TIVTLLSPSRGKFNVTDKGGLLEKGYFDLGAVYPNIILGLIMFGGLARGVYELSFGHLDQ 1620 TIVTLLSPSRGKFNVTDKGGLLEKGYFDLGAVYPNIILGLIMFGGLARGVYELSFGHLDQ Sbjct 481 TIVTLLSPSRGKFNVTDKGGLLEKGYFDLGAVYPNIILGLIMFGGLARGVYELSFGHLDQ 540 Query 1621 IAERAYLLNSAWamlsliiilaaiaVGRETQQKRNSHRIPATIPVEVANADGSIIVTGVT 1800 IAERAYLLNSAWAMLSLIIILAAIA VGRETQQKRNSHRIPATIPVEVANADGSIIVTGVT Sbjct 541 IAERAYLLNSAWAMLSLIIILAAIA VGRETQQKRNSHRIPATIPVEVANADGSIIVTGVT 600 Query 1801 EDLSMGGAAVKMSWPAKLSGPTPVYIRTVLDGEELILPARIIRAGNGRGIFIWTIDNLQQ 1980 EDLSMGGAAVKMSWPAKLSGPTPVYIRTVLDGEELILPARIIRAGNGRGIFIWTIDNLQQ Sbjct 601 EDLSMGGAAVKMSWPAKLSGPTPVYIRTVLDGEELILPARIIRAGNGRGIFIWTIDNLQQ 660 Query 1981 EFSVIRLVFGRADAWVDWGNYKADRPLLSLMDMVLSVKGLFRSSGDIVHRSSPTKPSAGN 2160 EFSVIRLVFGRADAWVDWGNYKADRPLLSLMDMVLSVKGLFRSSGDIVHRSSPTKPSAGN Sbjct 661 EFSVIRLVFGRADAWVDWGNYKADRPLLSLMDMVLSVKGLFRSSGDIVHRSSPTKPSAGN 720 Query 2161 ALSDDTNNPSRKERVLKGTVKMVsllalltfassaqaasapravaakapahqpeasDlpp 2340 ALSDDTNNPSRKERVLKGTVKMVSLLALLTFASSAQAASAPRAVAAKAPAHQPEASDLPP Sbjct 721 ALSDDTNNPSRKERVLKGTVKMVSLLALLTFASSAQAASAPRAVAAKAPAHQPEASDLPP 780 Query 2341 lpallpatsgaaqagsgdagadgpgsptgQPLAADSADALVENAENTSDTATVHNYTLKD 2520 LPALLPATSGAAQAGSGDAGADGPGSPTGQPLAADSADALVENAENTSDTATVHNYTLKD Sbjct 781 LPALLPATSGAAQAGSGDAGADGPGSPTGQPLAADSADALVENAENTSDTATVHNYTLKD 840 Query 2521 LGAAGSITMRGLAPLQGIEFGIPSDQLVTSARLVLSGSMSPNLRPETNSVTMTLNEQYIG 2700 LGAAGSITMRGLAPLQGIEFGIPSDQLVTSARLVLSGSMSPNLRPETNSVTMTLNEQYIG Sbjct 841 LGAAGSITMRGLAPLQGIEFGIPSDQLVTSARLVLSGSMSPNLRPETNSVTMTLNEQYIG 900 Query 2701 TLRPDPAHPTFGPMSFEINPIFFVSGNRLNFNFASGSKGCSDITNDTLWATISQNSQLQI 2880 TLRPDPAHPTFGPMSFEINPIFFVSGNRLNFNFASGSKGCSDITNDTLWATISQNSQLQI Sbjct 901 TLRPDPAHPTFGPMSFEINPIFFVSGNRLNFNFASGSKGCSDITNDTLWATISQNSQLQI 960 Query 2881 TTIAlpprrllsrlpqpFYDKNVRQHVTVPMVLAQTYDPQILKSAGILASWFGKQTDFLG 3060 TTIALPPRRLLSRLPQPFYDKNVRQHVTVPMVLAQTYDPQILKSAGILASWFGKQTDFLG Sbjct 961 TTIALPPRRLLSRLPQPFYDKNVRQHVTVPMVLAQTYDPQILKSAGILASWFGKQTDFLG 1020 Query 3061 VTFPVSSTIPQSGNAILIGVADELPTSFGRPQVNGPAVLELPNPSDANATILVVTGRDRD 3240 VTFPVSSTIPQSGNAILIGVADELPTSFGRPQVNGPAVLELPNPSDANATILVVTGRDRD Sbjct 1021 VTFPVSSTIPQSGNAILIGVADELPTSFGRPQVNGPAVLELPNPSDANATILVVTGRDRD 1080 Query 3241 EVITASKGIAFASAPLPTDSHMDVAPVDIAPRKPNDAPSFIAMDHPVRFGDLVTASKLQG 3420 EVITASKGIAFASAPLPTDSHMDVAPVDIAPRKPNDAPSFIAMDHPVRFGDLVTASKLQG Sbjct 1081 EVITASKGIAFASAPLPTDSHMDVAPVDIAPRKPNDAPSFIAMDHPVRFGDLVTASKLQG 1140 Query 3421 TGFTSGVLSVPFRIPPDLYTWRNRPYKMQVRFRSPAGEAKDVEKSRLDVGINEVYLHSYP 3600 TGFTSGVLSVPFRIPPDLYTWRNRPYKMQVRFRSPAGEAKDVEKSRLDVGINEVYLHSYP Sbjct 1141 TGFTSGVLSVPFRIPPDLYTWRNRPYKMQVRFRSPAGEAKDVEKSRLDVGINEVYLHSYP 1200 Query 3601 LRETHGLIGAVLQGVGLARPASGMQVHDLDVPPWTVFGQDQLNFYFDAMPLARGICQSGA 3780 LRETHGLIGAVLQGVGLARPASGMQVHDLDVPPWTVFGQDQLNFYFDAMPLARGICQSGA Sbjct 1201 LRETHGLIGAVLQGVGLARPASGMQVHDLDVPPWTVFGQDQLNFYFDAMPLARGICQSGA 1260 Query 3781 ANNAFHLGLDPDSTIDFSRAHHIAQMPNLAYMATVGFPFTTYADLSQTAVVLPEHPNAAT 3960 ANNAFHLGLDPDSTIDFSRAHHIAQMPNLAYMATVGFPFTTYADLSQTAVVLPEHPNAAT Sbjct 1261 ANNAFHLGLDPDSTIDFSRAHHIAQMPNLAYMATVGFPFTTYADLSQTAVVLPEHPNAAT 1320 Query 3961 VGAYLDLMGFMGAATWYPVAGVDIVSADHVSDVADRNLLVISTLATSGEIAPLLSRSSYE 4140 VGAYLDLMGFMGAATWYPVAGVDIVSADHVSDVADRNLLVISTLATSGEIAPLLSRSSYE Sbjct 1321 VGAYLDLMGFMGAATWYPVAGVDIVSADHVSDVADRNLLVISTLATSGEIAPLLSRSSYE 1380 Query 4141 VADGHLRTVSHASALDNAIKAVDDPLTAFRDRDSKPQDVDTPLTGGVGAMIEAESPLTAG 4320 VADGHLRTVSHASALDNAIKAVDDPLTAFRDRDSKPQDVDTPLTGGVGAMIEAESPLTAG Sbjct 1381 VADGHLRTVSHASALDNAIKAVDDPLTAFRDRDSKPQDVDTPLTGGVGAMIEAESPLTAG 1440 Query 4321 RTVLALLSSDGAGLNNLLQMLGERKKQANIQGDLVVAHGEDLSSYRTSPVYTIGTLPLWL 4500 RTVLALLSSDGAGLNNLLQMLGERKKQANIQGDLVVAHGEDLSSYRTSPVYTIGTLPLWL Sbjct 1441 RTVLALLSSDGAGLNNLLQMLGERKKQANIQGDLVVAHGEDLSSYRTSPVYTIGTLPLWL 1500 Query 4501 WPDWYMHNRPvrvllvgllgcilivsvlaralarhaarrFKQLEDERRKS 4650 WPDWYMHNRPVRVLLVGLLGCILIVSVLARALARHAARRFKQLEDERRKS Sbjct 1501 WPDWYMHNRPVRVLLVGLLGCILIVSVLARALARHAARRFKQLEDERRKS 1550 GenBank sequence AB091060.1 teaches an acsC gene (second FEATURE) that encodes a protein with 99% identity to the protein encoded by instant SEQ ID NO: 4. See alignment below. Score Expect Method Identities Positives Gaps Frame 2421 bits(6275) 0.0 Compositional matrix adjust. 1297/1302(99%) 1298/1302(99%) 0/1302(0%) +1 Query 1 MTHKRYASSLSAGLLATTCVaglllqangaraqqaaeaqapaSSTTMMQAATVAPAQSGq 180 MTHKRYASSLSAGLLATTCVAGLLLQANGARAQQAAEAQAPASSTTMMQAATVAPAQSGQ Sbjct 1 MTHKRYASSLSAGLLATTCVAGLLLQANGARAQQAAEAQAPASSTTMMQAATVAPAQSGQ 60 Query 181 aavvqrlvqqarFWMQQHQYENARQSLQSAARLAPDSVDLLEAEGEYQSHIGNRDAALDT 360 AAVVQRLVQQARFWMQQHQYENARQSLQSAARLAPDSVDLLEAEGEYQSHIGNRDAALDT Sbjct 61 AAVVQRLVQQARFWMQQHQYENARQSLQSAARLAPDSVDLLEAEGEYQSHIGNRDAALDT 120 Query 361 QRRLHQAAPGSTYESQLNDLLHEQAISQPDLAHARSLAASGHSDQAVEAYQHLFNGSTPT 540 QRRLHQAAPGSTYESQLNDLLHEQAISQPDLAHARSLAASGHSDQAVEAYQHLFNGSTPT Sbjct 121 QRRLHQAAPGSTYESQLNDLLHEQAISQPDLAHARSLAASGHSDQAVEAYQHLFNGSTPT 180 Query 541 PSLAVEYYQTLAGVSGQAGTAQDGLIRLVKANPSDFRAQLALAQVLTYQPGTRMEGLQRL 720 PSLAVEYYQTLA VSGQAGTAQDGLIRLVKANPSDFRAQLALAQVLTYQPGTRMEGLQRL Sbjct 181 PSLAVEYYQTLADVSGQAGTAQDGLIRLVKANPSDFRAQLALAQVLTYQPGTRMEGLQRL 240 Query 721 QALQKYQSSAPVEAATAEKSYRQTLSWLPVTPETLPLMQKWLDAHPSDSALRTHMAEPAG 900 QALQKYQSSAPVEAATAEKSYRQTLSWLPVTPETLPLMQKWLDAHPSDSALRTHMAEPAG Sbjct 241 QALQKYQSSAPVEAATAEKSYRQTLSWLPVTPETLPLMQKWLDAHPSDSALRTHMAEPAG 300 Query 901 GPPDKGALARQDGFKALNAGRLsaaqaafqsalnlnaKDGDAlgglglVAMRAGHNEEAH 1080 GPPDKGALARQDGFKALNAGRLSAAQAAFQSALNLNAKDGDALGGLGLVAMRAGHNEEAH Sbjct 301 GPPDKGALARQDGFKALNAGRLSAAQAAFQSALNLNAKDGDALGGLGLVAMRAGHNEEAH 360 Query 1081 RYLEDAIA ADPKNAAHWRPALAGMAVGEEYGSVRRLIASGQTQEAEQRLMTLARQPGQSE 1260 RYLEDAIA ADPKNAAHWRPALAGMAVGEEYGSVRRLIASGQTQEAEQRLMTLARQPGQSE Sbjct 361 RYLEDAIA ADPKNAAHWRPALAGMAVGEEYGSVRRLIASGQTQEAEQRLMTLARQPGQSE 420 Query 1261 GATLMLADLQRSTGQTGEAERNYRAILARNGDNPIALMGLARVLMGEGQESEANALLSRL 1440 GATLMLADLQRSTGQTGEAERNYRAILARNGDNPIALMGLARVLMGEGQE+EANALLSRL Sbjct 421 GATLMLADLQRSTGQTGEAERNYRAILARNGDNPIALMGLARVLMGEGQENEANALLSRL 480 Query 1441 GGRYSDQVQQIEVSGIMAEAARTSDSAQKVSLLRQAMTKAPDDPWLRINLANALQQQGDS 1620 GGRYSDQVQQIEVSGIMAEAARTSDSAQKVSLLRQAMTKAPDDPWLRINLANALQQQGDS Sbjct 481 GGRYSDQVQQIEVSGIMAEAARTSDSAQKVSLLRQAMTKAPDDPWLRINLANALQQQGDS 540 Query 1621 AEAANVMRPLLTSPRTPADYQAAILYASGNGNDTLARRLLAGLSPDDYSPAIRTIADEMA 1800 AEAANVMRPLLTSPRTPADYQAAILYASGNGNDTLARRLLAGLSPDDYSPAIRTIADEMA Sbjct 541 AEAANVMRPLLTSPRTPADYQAAILYASGNGNDTLARRLLAGLSPDDYSPAIRTIADEMA 600 Query 1801 IKADLASRLSMVSNPTPLVREALAAPDPTGARGVAVADLFRQRGDMLHAHMALRIASTRN 1980 IKADLASRLSMVSNPTPLVREALAAPDPTGARGVAVADLFRQRGDMLHAHMALRIASTRN Sbjct 601 IKADLASRLSMVSNPTPLVREALAAPDPTGARGVAVADLFRQRGDMLHAHMALRIASTRN 660 Query 1981 IDLTTEQRLAYATEYMKISNPVAAARLLAPLGDGSGTATGSAMSPDQRQTLMQLRMGISV 2160 IDLTTEQRLAYATEYMKISNPVAAARLLAPLGDGSGTATGSAMSPDQRQTLMQLRMGISV Sbjct 661 IDLTTEQRLAYATEYMKISNPVAAARLLAPLGDGSGTATGSAMSPDQRQTLMQLRMGISV 720 Query 2161 AQSDLLNQRGDQAAAYDHLAPALQADPEATSPKLALARLYNGRGKYGHALDIDLAVLRHN 2340 AQSDLLNQRGDQAAA DHLAPALQADPEATSPKLALARLYNGRGKYGHALDIDLAVLRHN Sbjct 721 AQSDLLNQRGDQAAACDHLAPALQADPEATSPKLALARLYNGRGKYGHALDIDLAVLRHN 780 Query 2341 PqdldarqaavqaaandGKDNLAMQLAQDGVQQSPMDARSWLGMAVADRAVGHGDRTLAD 2520 PQDLDARQAAVQAAANDGKDNLAMQLAQDGVQQSPMDARSWLGMAVAD AVGHGDRTLAD Sbjct 781 PQDLDARQAAVQAAANDGKDNLAMQLAQDGVQQSPMDARSWLGMAVADHAVGHGDRTLAD 840 Query 2521 LRRAYELRLQQLKISRGDAIGGDETQATAPPTANPFRRDAYGHALSLGAPPGENGYSTAG 2700 LRRAYELRLQQLKISRGDAIGGDETQATAPPTANPFRRDAYGHALSLGAPPGENGYSTAG Sbjct 841 LRRAYELRLQQLKISRGDAIGGDETQATAPPTANPFRRDAYGHALSLGAPPGENGYSTAG 900 Query 2701 SVPEISDQMLSSINGQIHTLSEDMAPSVDAGLGFRVRSGTPGMGALTEASVPIVGRIPLQ 2880 SVPEISDQMLSSINGQIHTLSEDMAPSVDAGLGFRVRSGTPGMGALTEASVPIVGRIPLQ Sbjct 901 SVPEISDQMLSSINGQIHTLSEDMAPSVDAGLGFRVRSGTPGMGALTEASVPIVGRIPLQ 960 Query 2881 AgtsaltftatptfltsgHLPQTGYDIPRFGTNLFALERNLQNQNNSAEHRINTDTIGRE 3060 AGTSALTFTATPTFLTSGHLPQTGYDIPRFGTNLFALERNLQNQNNSAEHRINTDTIGRE Sbjct 961 AGTSALTFTATPTFLTSGHLPQTGYDIPRFGTNLFALERNLQNQNNSAEHRINTDTIGRE 1020 Query 3061 AGVAPDVRFANNWVSADVGASPLGFTLPNVIGGVEFAPRVGPVTFRVSGERRSITNSVLS 3240 AGVAPDVRFANNWVSADVGASPLGFTLPNVIGGVEFAPRVGPVTFRVSGERRSITNSVLS Sbjct 1021 AGVAPDVRFANNWVSADVGASPLGFTLPNVIGGVEFAPRVGPVTFRVSGERRSITNSVLS 1080 Query 3241 YGGMTDALTGKKWGGVVTNHFHGQVEATLGNTIVYGGGGYAIQTGHHVQSNTEVEGGLGA 3420 YGGMTDALTGKKWGGVVTNHFHGQVEATLGNTIVYGGGGYAIQTGHHVQSNTEVEGGLGA Sbjct 1081 YGGMTDALTGKKWGGVVTNHFHGQVEATLGNTIVYGGGGYAIQTGHHVQSNTEVEGGLGA 1140 Query 3421 NTLVYRNRKHEVRVGVNLTYFGYKHNEDFYTYGQGGYFSPQSYFAATVPVRYSGHSGLFD 3600 NTLVYRNRKHEVRVGVNLTY GYKHNEDFYTYGQGGYFSPQSYFAATVPVRYSGHSGLFD Sbjct 1141 NTLVYRNRKHEVRVGVNLTYLGYKHNEDFYTYGQGGYFSPQSYFAATVPVRYSGHSGLFD 1200 Query 3601 WDVTGSIGYQLFHEHSSAFFPTNPVYQALANGLAGVSTAELSLESARYPGDDVGSLVGGF 3780 WDVTGSIGYQLFHEHSSAFFPTNPVYQALANGLAGVSTAELSLESARYPGDDVGSLVGGF Sbjct 1201 WDVTGSIGYQLFHEHSSAFFPTNPVYQALANGLAGVSTAELSLESARYPGDDVGSLVGGF 1260 Query 3781 DGRVGYRVSHSLRLDLSGRFQKAGNWDEGGAMISAHYLIMDQ 3906 DGRVGYRVSHSLRLDLSGRFQKAGNWDEGGAMISAHYLIMDQ Sbjct 1261 DGRVGYRVSHSLRLDLSGRFQKAGNWDEGGAMISAHYLIMDQ 1302 Finally, GenBank sequence AB091060.1 teaches an acsD gene (third FEATURE) which has 100% sequence identity to instant SEQ ID NO: 5 (first alignment below) encoding a protein with 100% identity to the protein encoded by instant SEQ ID NO: 6 (second alignment below). The acsAB, acsC, and acsD genes are all present sequentially and in the same orientation within GenBank sequence AB091060.1. SEQ ID NO: 5 vs. GenBank sequence AB091060.1 Score Expect Identities Gaps Strand 870 bits(471) 0.0 471/471(100%) 0/471(0%) Plus/Plus Query 1 ATGACAATTTTTGAGAAAAAACCGGATTTCACCCTGTTTCTTCAGACCCTGTCATGGGAA 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8645 ATGACAATTTTTGAGAAAAAACCGGATTTCACCCTGTTTCTTCAGACCCTGTCATGGGAA 8704 Query 61 ATTGATGATCAGGTCGGGATCGAGGTCAGGAACGAGCTCCTGCGTGAGGTCGGACGGGGC 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8705 ATTGATGATCAGGTCGGGATCGAGGTCAGGAACGAGCTCCTGCGTGAGGTCGGACGGGGC 8764 Query 121 ATGGGCACGCGCATCATGCCGCCGCCGTGCCAGACCGTGGACAAGCTGCAGATCGAACTG 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8765 ATGGGCACGCGCATCATGCCGCCGCCGTGCCAGACCGTGGACAAGCTGCAGATCGAACTG 8824 Query 181 AACGCGCTTCTGGCCCTGATCGGCTGGGGCACCGTGACGCTCGAACTCCTCAGCGAGGAC 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8825 AACGCGCTTCTGGCCCTGATCGGCTGGGGCACCGTGACGCTCGAACTCCTCAGCGAGGAC 8884 Query 241 CAGTCCCTGCGCATCGTGCATGAAAACCTGCCGCAGGTTGGCAGCGCGGGCGAACCTTCG 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8885 CAGTCCCTGCGCATCGTGCATGAAAACCTGCCGCAGGTTGGCAGCGCGGGCGAACCTTCG 8944 Query 301 GGCACGTGGCTGGCTCCGGTGCTGGAGGGGCTGTATGGCCGCTGGGTGACGTCGCAGGCG 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8945 GGCACGTGGCTGGCTCCGGTGCTGGAGGGGCTGTATGGCCGCTGGGTGACGTCGCAGGCG 9004 Query 361 GGTGCGTTTGGTGATTATGTCGTTACACGCGATGTGGACGCCGAGGATCTGAATGCTGTT 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 9005 GGTGCGTTTGGTGATTATGTCGTTACACGCGATGTGGACGCCGAGGATCTGAATGCTGTT 9064 Query 421 CCGCGTCAGACCATCATCATGTACATGCGCGTGCGCAGTTCCGCGACCTGA 471 ||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 9065 CCGCGTCAGACCATCATCATGTACATGCGCGTGCGCAGTTCCGCGACCTGA 9115 Translated SEQ ID NO: 6 vs GenBank sequence AB091060.1 acsD protein Score Expect Method Identities Positives Gaps Frame 318 bits(814) 2e-118 Compositional matrix adjust. 156/156(100%) 156/156(100%) 0/156(0%) +1 Query 1 MTIFEKKPDFTLFLQTLSWEIDDQVGIEVRNELLREVGRGMGTRIMPPPCQTVDKLQIEL 180 MTIFEKKPDFTLFLQTLSWEIDDQVGIEVRNELLREVGRGMGTRIMPPPCQTVDKLQIEL Sbjct 1 MTIFEKKPDFTLFLQTLSWEIDDQVGIEVRNELLREVGRGMGTRIMPPPCQTVDKLQIEL 60 Query 181 NALLALIGWGTVTLELLSEDQSLRIVHENLPQVGSAGEPSGTWLAPVLEGLYGRWVTSQA 360 NALLALIGWGTVTLELLSEDQSLRIVHENLPQVGSAGEPSGTWLAPVLEGLYGRWVTSQA Sbjct 61 NALLALIGWGTVTLELLSEDQSLRIVHENLPQVGSAGEPSGTWLAPVLEGLYGRWVTSQA 120 Query 361 GAFGDYVVTRDVDAEDLNAVPRQTIIMYMRVRSSAT 468 GAFGDYVVTRDVDAEDLNAVPRQTIIMYMRVRSSAT Sbjct 121 GAFGDYVVTRDVDAEDLNAVPRQTIIMYMRVRSSAT 156 GenBank sequence X54676.1 teaches an Acetobacter xylinum gene for cellulose biosynthesis (see DEFINITION) from strain ATCC 53582 (see FEATURES source). The sequence comprises acsA, acsB, acsC, and acsD genes (see FEATURES). GenBank sequence X54676.1 teaches a sequence that comprises a sequence with 100% sequence identity to instant SEQ ID NO: 3, encoding an acsC protein with 100% identity to the protein encoded by instant SEQ ID NO: 4. See alignments below. SEQ ID NO: 3 vs GenBank sequence X54676.1 Score Expect Identities Gaps Strand 7219 bits(3909) 0.0 3909/3909(100%) 0/3909(0%) Plus/Plus Query 1 GTGACCCATAAACGATATGCTTCGTCCCTGTCCGCCGGTCTTCTCGCAACGACCTGCGTC 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5286 GTGACCCATAAACGATATGCTTCGTCCCTGTCCGCCGGTCTTCTCGCAACGACCTGCGTC 5345 Query 61 GCAGGTCTGTTGCTGCAGGCGAACGGCGCACGGGCACAGCAGGCGGCAGAGGCGCAGGCC 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5346 GCAGGTCTGTTGCTGCAGGCGAACGGCGCACGGGCACAGCAGGCGGCAGAGGCGCAGGCC 5405 Query 121 CCGGCCAGCAGCACCACCATGATGCAAGCAGCCACCGTTGCCCCCGCCCAGAGTGGGCAG 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5406 CCGGCCAGCAGCACCACCATGATGCAAGCAGCCACCGTTGCCCCCGCCCAGAGTGGGCAG 5465 Query 181 GCCGCGGTGGTGCAGCGGCTGGTGCAGCAGGCCCGTTTCTGGATGCAGCAGCACCAGTAT 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5466 GCCGCGGTGGTGCAGCGGCTGGTGCAGCAGGCCCGTTTCTGGATGCAGCAGCACCAGTAT 5525 Query 241 GAAAATGCGCGCCAGTCCCTGCAGAGTGCTGCGCGACTTGCACCGGATTCCGTTGATCTG 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5526 GAAAATGCGCGCCAGTCCCTGCAGAGTGCTGCGCGACTTGCACCGGATTCCGTTGATCTG 5585 Query 301 CTGGAGGCCGAGGGCGAATACCAGTCGCATATCGGCAATCGTGATGCCGCCCTTGATACG 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5586 CTGGAGGCCGAGGGCGAATACCAGTCGCATATCGGCAATCGTGATGCCGCCCTTGATACG 5645 Query 361 CAGCGTCGCCTGCATCAGGCCGCACCTGGCAGCACGTATGAAAGCCAGTTGAACGACCTG 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5646 CAGCGTCGCCTGCATCAGGCCGCACCTGGCAGCACGTATGAAAGCCAGTTGAACGACCTG 5705 Query 421 CTGCATGAACAGGCGATTTCCCAGCCGGACCTTGCGCATGCGCGCTCGCTTGCCGCATCC 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5706 CTGCATGAACAGGCGATTTCCCAGCCGGACCTTGCGCATGCGCGCTCGCTTGCCGCATCC 5765 Query 481 GGGCACAGCGATCAGGCGGTGGAAGCGTACCAGCACCTGTTCAACGGTTCGACGCCCACG 540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5766 GGGCACAGCGATCAGGCGGTGGAAGCGTACCAGCACCTGTTCAACGGTTCGACGCCCACG 5825 Query 541 CCTTCGCTCGCGGTTGAATATTACCAGACGCTGGCTGGCGTCTCGGGGCAGGCCGGTACG 600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5826 CCTTCGCTCGCGGTTGAATATTACCAGACGCTGGCTGGCGTCTCGGGGCAGGCCGGTACG 5885 Query 601 GCACAGGATGGGCTGATCCGTCTGGTCAAGGCCAATCCTTCCGATTTCCGGGCGCAACTG 660 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5886 GCACAGGATGGGCTGATCCGTCTGGTCAAGGCCAATCCTTCCGATTTCCGGGCGCAACTG 5945 Query 661 GCGCTTGCACAGGTCCTGACCTATCAGCCCGGCACCCGGATGGAGGGGCTGCAGCGGCTT 720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 5946 GCGCTTGCACAGGTCCTGACCTATCAGCCCGGCACCCGGATGGAGGGGCTGCAGCGGCTT 6005 Query 721 CAGGCGCTCCAGAAGTACCAGTCTTCCGCCCCGGTGGAGGCTGCGACGGCGGAAAAATCA 780 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6006 CAGGCGCTCCAGAAGTACCAGTCTTCCGCCCCGGTGGAGGCTGCGACGGCGGAAAAATCA 6065 Query 781 TATCGCCAGACGCTGTCATGGTTACCGGTTACACCCGAAACACTGCCGTTGATGCAGAAA 840 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6066 TATCGCCAGACGCTGTCATGGTTACCGGTTACACCCGAAACACTGCCGTTGATGCAGAAA 6125 Query 841 TGGCTGGATGCACATCCATCCGACAGCGCATTGCGGACCCATATGGCAGAACCGGCAGGC 900 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6126 TGGCTGGATGCACATCCATCCGACAGCGCATTGCGGACCCATATGGCAGAACCGGCAGGC 6185 Query 901 GGGCCGCCGGATAAAGGCGCGCTGGCGCGGCAGGACGGGTTCAAGGCGTTGAACGCCGGA 960 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6186 GGGCCGCCGGATAAAGGCGCGCTGGCGCGGCAGGACGGGTTCAAGGCGTTGAACGCCGGA 6245 Query 961 CGTCTGTCCGCAGCCCAGGCCGCGTTCCAGAGCGCACTGAACCTGAATGCCAAGGACGGC 1020 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6246 CGTCTGTCCGCAGCCCAGGCCGCGTTCCAGAGCGCACTGAACCTGAATGCCAAGGACGGC 6305 Query 1021 GATGCCCTGGGCGGCCTTGGCCTTGTCGCCATGCGCGCGGGCCATAACGAGGAAGCGCAT 1080 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6306 GATGCCCTGGGCGGCCTTGGCCTTGTCGCCATGCGCGCGGGCCATAACGAGGAAGCGCAT 6365 Query 1081 CGTTACCTCGAAGATGCGATCGCGGCTGATCCCAAGAATGCGGCGCACTGGCGTCCGGCA 1140 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6366 CGTTACCTCGAAGATGCGATCGCGGCTGATCCCAAGAATGCGGCGCACTGGCGTCCGGCA 6425 Query 1141 CTGGCTGGCATGGCCGTGGGCGAGGAATATGGCAGCGTCCGTCGCCTGATCGCCAGTGGA 1200 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6426 CTGGCTGGCATGGCCGTGGGCGAGGAATATGGCAGCGTCCGTCGCCTGATCGCCAGTGGA 6485 Query 1201 CAGACACAGGAAGCCGAACAGCGCCTGATGACGCTGGCGCGTCAGCCCGGACAGTCCGAG 1260 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6486 CAGACACAGGAAGCCGAACAGCGCCTGATGACGCTGGCGCGTCAGCCCGGACAGTCCGAG 6545 Query 1261 GGCGCGACCCTTATGCTGGCGGATTTGCAGCGCAGCACGGGCCAGACCGGCGAGGCCGAG 1320 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6546 GGCGCGACCCTTATGCTGGCGGATTTGCAGCGCAGCACGGGCCAGACCGGCGAGGCCGAG 6605 Query 1321 CGCAATTACCGGGCGATCCTGGCGCGCAATGGTGACAATCCCATCGCACTGATGGGACTG 1380 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6606 CGCAATTACCGGGCGATCCTGGCGCGCAATGGTGACAATCCCATCGCACTGATGGGACTG 6665 Query 1381 GCCCGTGTGCTGATGGGTGAAGGCCAGGAGAGCGAAGCGAACGCCCTGCTGTCGCGCCTT 1440 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6666 GCCCGTGTGCTGATGGGTGAAGGCCAGGAGAGCGAAGCGAACGCCCTGCTGTCGCGCCTT 6725 Query 1441 GGTGGTCGCTATAGCGACCAGGTACAGCAGATCGAGGTTTCGGGCATCATGGCCGAAGCC 1500 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6726 GGTGGTCGCTATAGCGACCAGGTACAGCAGATCGAGGTTTCGGGCATCATGGCCGAAGCC 6785 Query 1501 GCCCGCACGTCGGATTCAGCGCAGAAGGTCAGCCTTCTGCGGCAGGCCATGACCAAGGCC 1560 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6786 GCCCGCACGTCGGATTCAGCGCAGAAGGTCAGCCTTCTGCGGCAGGCCATGACCAAGGCC 6845 Query 1561 CCGGATGACCCGTGGTTGCGCATCAACCTTGCCAATGCGCTGCAACAGCAAGGTGACAGC 1620 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6846 CCGGATGACCCGTGGTTGCGCATCAACCTTGCCAATGCGCTGCAACAGCAAGGTGACAGC 6905 Query 1621 GCGGAAGCGGCCAATGTCATGCGTCCGCTTCTGACCAGCCCGCGTACGCCGGCCGATTAT 1680 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6906 GCGGAAGCGGCCAATGTCATGCGTCCGCTTCTGACCAGCCCGCGTACGCCGGCCGATTAT 6965 Query 1681 CAGGCGGCGATCCTGTATGCGTCCGGCAATGGCAATGATACGCTGGCGCGCCGTTTGCTT 1740 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6966 CAGGCGGCGATCCTGTATGCGTCCGGCAATGGCAATGATACGCTGGCGCGCCGTTTGCTT 7025 Query 1741 GCGGGACTGTCGCCGGATGACTATTCCCCGGCCATCCGCACCATTGCCGATGAAATGGCG 1800 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7026 GCGGGACTGTCGCCGGATGACTATTCCCCGGCCATCCGCACCATTGCCGATGAAATGGCG 7085 Query 1801 ATCAAGGCCGATCTGGCCAGCCGCCTTTCGATGGTTTCGAATCCCACGCCGCTTGTGCGC 1860 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7086 ATCAAGGCCGATCTGGCCAGCCGCCTTTCGATGGTTTCGAATCCCACGCCGCTTGTGCGC 7145 Query 1861 GAGGCTCTGGCCGCGCCGGACCCGACGGGCGCGCGTGGTGTGGCGGTGGCCGACCTGTTC 1920 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7146 GAGGCTCTGGCCGCGCCGGACCCGACGGGCGCGCGTGGTGTGGCGGTGGCCGACCTGTTC 7205 Query 1921 CGGCAGCGTGGTGACATGCTGCATGCGCATATGGCGCTGCGCATCGCCTCCACGCGCAAT 1980 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7206 CGGCAGCGTGGTGACATGCTGCATGCGCATATGGCGCTGCGCATCGCCTCCACGCGCAAT 7265 Query 1981 ATTGACCTGACGACCGAACAGCGGCTGGCCTATGCCACCGAATATATGAAGATCAGCAAT 2040 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7266 ATTGACCTGACGACCGAACAGCGGCTGGCCTATGCCACCGAATATATGAAGATCAGCAAT 7325 Query 2041 CCCGTTGCCGCAGCACGCCTGCTTGCACCGTTGGGCGATGGCAGCGGGACCGCAACCGGT 2100 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7326 CCCGTTGCCGCAGCACGCCTGCTTGCACCGTTGGGCGATGGCAGCGGGACCGCAACCGGT 7385 Query 2101 TCCGCGATGTCGCCGGATCAGCGGCAGACGCTGATGCAGTTGCGCATGGGTATTTCGGTG 2160 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7386 TCCGCGATGTCGCCGGATCAGCGGCAGACGCTGATGCAGTTGCGCATGGGTATTTCGGTG 7445 Query 2161 GCACAGTCGGACCTGCTGAACCAGCGTGGCGATCAGGCGGCGGCGTATGACCATCTTGCG 2220 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7446 GCACAGTCGGACCTGCTGAACCAGCGTGGCGATCAGGCGGCGGCGTATGACCATCTTGCG 7505 Query 2221 CCCGCGTTGCAGGCCGATCCAGAGGCGACATCCCCCAAGCTTGCGTTGGCGCGACTGTAT 2280 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7506 CCCGCGTTGCAGGCCGATCCAGAGGCGACATCCCCCAAGCTTGCGTTGGCGCGACTGTAT 7565 Query 2281 AACGGGCGTGGCAAGTATGGGCACGCGCTGGACATCGACCTTGCTGTTCTTCGGCATAAT 2340 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7566 AACGGGCGTGGCAAGTATGGGCACGCGCTGGACATCGACCTTGCTGTTCTTCGGCATAAT 7625 Query 2341 CCGCAGGACCTTGATGCGCGGCAGGCCGCCGTGCAGGCCGCGGCGAATGACGGCAAGGAC 2400 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7626 CCGCAGGACCTTGATGCGCGGCAGGCCGCCGTGCAGGCCGCGGCGAATGACGGCAAGGAC 7685 Query 2401 AACCTGGCCATGCAACTGGCGCAAGATGGGGTCCAGCAGTCGCCGATGGATGCGCGCAGC 2460 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7686 AACCTGGCCATGCAACTGGCGCAAGATGGGGTCCAGCAGTCGCCGATGGATGCGCGCAGC 7745 Query 2461 TGGCTTGGCATGGCGGTGGCGGACCGCGCTGTGGGCCATGGCGACCGGACGCTTGCTGAC 2520 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7746 TGGCTTGGCATGGCGGTGGCGGACCGCGCTGTGGGCCATGGCGACCGGACGCTTGCTGAC 7805 Query 2521 CTGCGCCGGGCGTATGAATTGCGCCTGCAGCAGCTCAAGATCAGTCGGGGTGATGCCATC 2580 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7806 CTGCGCCGGGCGTATGAATTGCGCCTGCAGCAGCTCAAGATCAGTCGGGGTGATGCCATC 7865 Query 2581 GGCGGTGATGAAACGCAGGCGACCGCGCCGCCGACGGCCAATCCGTTCCGTCGCGATGCA 2640 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7866 GGCGGTGATGAAACGCAGGCGACCGCGCCGCCGACGGCCAATCCGTTCCGTCGCGATGCA 7925 Query 2641 TACGGGCATGCGCTGTCGTTGGGTGCGCCCCCGGGTGAGAACGGGTACAGCACGGCAGGA 2700 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7926 TACGGGCATGCGCTGTCGTTGGGTGCGCCCCCGGGTGAGAACGGGTACAGCACGGCAGGA 7985 Query 2701 AGCGTGCCCGAGATTTCGGACCAGATGCTGTCCTCCATCAACGGGCAGATCCATACCCTG 2760 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7986 AGCGTGCCCGAGATTTCGGACCAGATGCTGTCCTCCATCAACGGGCAGATCCATACCCTG 8045 Query 2761 TCCGAAGACATGGCGCCGTCTGTTGATGCCGGCCTTGGCTTCCGTGTCCGTTCCGGCACC 2820 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8046 TCCGAAGACATGGCGCCGTCTGTTGATGCCGGCCTTGGCTTCCGTGTCCGTTCCGGCACC 8105 Query 2821 CCGGGCATGGGCGCGTTGACCGAGGCATCCGTGCCGATCGTCGGGCGCATCCCGCTGCAG 2880 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8106 CCGGGCATGGGCGCGTTGACCGAGGCATCCGTGCCGATCGTCGGGCGCATCCCGCTGCAG 8165 Query 2881 GCTGGTACTTCGGCACTGACATTCACGGCCACGCCAACCTTCCTGACGTCGGGGCACCTG 2940 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8166 GCTGGTACTTCGGCACTGACATTCACGGCCACGCCAACCTTCCTGACGTCGGGGCACCTG 8225 Query 2941 CCGCAGACCGGGTATGATATACCGCGTTTCGGCACCAATCTGTTCGCGCTGGAACGGAAC 3000 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8226 CCGCAGACCGGGTATGATATACCGCGTTTCGGCACCAATCTGTTCGCGCTGGAACGGAAC 8285 Query 3001 CTGCAGAACCAGAACAACAGCGCGGAACATCGCATCAATACCGATACGATCGGGCGCGAG 3060 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8286 CTGCAGAACCAGAACAACAGCGCGGAACATCGCATCAATACCGATACGATCGGGCGCGAG 8345 Query 3061 GCAGGTGTGGCGCCTGATGTCCGCTTTGCCAATAACTGGGTCAGTGCCGATGTCGGTGCC 3120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8346 GCAGGTGTGGCGCCTGATGTCCGCTTTGCCAATAACTGGGTCAGTGCCGATGTCGGTGCC 8405 Query 3121 TCGCCTCTGGGCTTTACGCTGCCGAACGTGATCGGTGGTGTTGAATTCGCCCCGCGTGTC 3180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8406 TCGCCTCTGGGCTTTACGCTGCCGAACGTGATCGGTGGTGTTGAATTCGCCCCGCGTGTC 8465 Query 3181 GGGCCTGTGACCTTCCGTGTCAGTGGGGAACGCCGCTCCATTACCAACAGCGTCCTGTCC 3240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8466 GGGCCTGTGACCTTCCGTGTCAGTGGGGAACGCCGCTCCATTACCAACAGCGTCCTGTCC 8525 Query 3241 TATGGCGGGATGACCGATGCCCTGACCGGCAAGAAGTGGGGCGGTGTTGTCACCAACCAC 3300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8526 TATGGCGGGATGACCGATGCCCTGACCGGCAAGAAGTGGGGCGGTGTTGTCACCAACCAC 8585 Query 3301 TTCCATGGACAGGTCGAGGCCACGCTGGGCAATACCATTGTGTATGGTGGCGGTGGTTAT 3360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8586 TTCCATGGACAGGTCGAGGCCACGCTGGGCAATACCATTGTGTATGGTGGCGGTGGTTAT 8645 Query 3361 GCCATCCAGACCGGCCATCATGTCCAGAGCAATACCGAAGTGGAAGGCGGGCTGGGGGCG 3420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8646 GCCATCCAGACCGGCCATCATGTCCAGAGCAATACCGAAGTGGAAGGCGGGCTGGGGGCG 8705 Query 3421 AATACCCTGGTTTATCGCAACCGCAAGCACGAAGTGCGTGTCGGTGTGAACCTGACCTAT 3480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8706 AATACCCTGGTTTATCGCAACCGCAAGCACGAAGTGCGTGTCGGTGTGAACCTGACCTAT 8765 Query 3481 TTCGGCTATAAGCATAACGAGGACTTCTATACTTACGGGCAGGGTGGCTACTTCTCGCCG 3540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8766 TTCGGCTATAAGCATAACGAGGACTTCTATACTTACGGGCAGGGTGGCTACTTCTCGCCG 8825 Query 3541 CAGTCCTATTTTGCGGCAACGGTGCCCGTCCGGTATTCCGGGCATAGTGGCCTGTTTGAC 3600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8826 CAGTCCTATTTTGCGGCAACGGTGCCCGTCCGGTATTCCGGGCATAGTGGCCTGTTTGAC 8885 Query 3601 TGGGATGTCACCGGGTCCATCGGTTATCAGTTGTTTCATGAACACAGTTCGGCCTTCTTC 3660 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8886 TGGGATGTCACCGGGTCCATCGGTTATCAGTTGTTTCATGAACACAGTTCGGCCTTCTTC 8945 Query 3661 CCGACCAATCCTGTGTATCAGGCCCTTGCGAACGGGCTGGCGGGTGTATCTACTGCTGAA 3720 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 8946 CCGACCAATCCTGTGTATCAGGCCCTTGCGAACGGGCTGGCGGGTGTATCTACTGCTGAA 9005 Query 3721 CTATCCTTGGAATCGGCCAGGTATCCCGGCGATGATGTCGGTAGCCTTGTTGGTGGTTTC 3780 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 9006 CTATCCTTGGAATCGGCCAGGTATCCCGGCGATGATGTCGGTAGCCTTGTTGGTGGTTTC 9065 Query 3781 GATGGCAGGGTGGGTTATCGCGTCAGCCATTCGCTGCGCCTTGATCTGTCCGGTCGTTTC 3840 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 9066 GATGGCAGGGTGGGTTATCGCGTCAGCCATTCGCTGCGCCTTGATCTGTCCGGTCGTTTC 9125 Query 3841 CAGAAGGCTGGAAACTGGGATGAGGGCGGCGCCATGATCTCGGCTCACTATCTTATTATG 3900 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 9126 CAGAAGGCTGGAAACTGGGATGAGGGCGGCGCCATGATCTCGGCTCACTATCTTATTATG 9185 Query 3901 GACCAGTAA 3909 ||||||||| Sbjct 9186 GACCAGTAA 9194 Translated SEQ ID NO: 4 vs GenBank sequence X54676.1 acsC Score Expect Method Identities Positives Gaps Frame 2433 bits(6305) 0.0 Compositional matrix adjust. 1302/1302(100%) 1302/1302(100%) 0/1302(0%) +1 Query 1 MTHKRYASSLSAGLLATTCVaglllqangaraqqaaeaqapaSSTTMMQAATVAPAQSGq 180 MTHKRYASSLSAGLLATTCVAGLLLQANGARAQQAAEAQAPASSTTMMQAATVAPAQSGQ Sbjct 1 MTHKRYASSLSAGLLATTCVAGLLLQANGARAQQAAEAQAPASSTTMMQAATVAPAQSGQ 60 Query 181 aavvqrlvqqarFWMQQHQYENARQSLQSAARLAPDSVDLLEAEGEYQSHIGNRDAALDT 360 AAVVQRLVQQARFWMQQHQYENARQSLQSAARLAPDSVDLLEAEGEYQSHIGNRDAALDT Sbjct 61 AAVVQRLVQQARFWMQQHQYENARQSLQSAARLAPDSVDLLEAEGEYQSHIGNRDAALDT 120 Query 361 QRRLHQAAPGSTYESQLNDLLHEQAISQPDLAHARSLAASGHSDQAVEAYQHLFNGSTPT 540 QRRLHQAAPGSTYESQLNDLLHEQAISQPDLAHARSLAASGHSDQAVEAYQHLFNGSTPT Sbjct 121 QRRLHQAAPGSTYESQLNDLLHEQAISQPDLAHARSLAASGHSDQAVEAYQHLFNGSTPT 180 Query 541 PSLAVEYYQTLAGVSGQAGTAQDGLIRLVKANPSDFRAQLALAQVLTYQPGTRMEGLQRL 720 PSLAVEYYQTLAGVSGQAGTAQDGLIRLVKANPSDFRAQLALAQVLTYQPGTRMEGLQRL Sbjct 181 PSLAVEYYQTLAGVSGQAGTAQDGLIRLVKANPSDFRAQLALAQVLTYQPGTRMEGLQRL 240 Query 721 QALQKYQSSAPVEAATAEKSYRQTLSWLPVTPETLPLMQKWLDAHPSDSALRTHMAEPAG 900 QALQKYQSSAPVEAATAEKSYRQTLSWLPVTPETLPLMQKWLDAHPSDSALRTHMAEPAG Sbjct 241 QALQKYQSSAPVEAATAEKSYRQTLSWLPVTPETLPLMQKWLDAHPSDSALRTHMAEPAG 300 Query 901 GPPDKGALARQDGFKALNAGRLsaaqaafqsalnlnaKDGDAlgglglVAMRAGHNEEAH 1080 GPPDKGALARQDGFKALNAGRLSAAQAAFQSALNLNAKDGDALGGLGLVAMRAGHNEEAH Sbjct 301 GPPDKGALARQDGFKALNAGRLSAAQAAFQSALNLNAKDGDALGGLGLVAMRAGHNEEAH 360 Query 1081 RYLEDAIA ADPKNAAHWRPALAGMAVGEEYGSVRRLIASGQTQEAEQRLMTLARQPGQSE 1260 RYLEDAIA ADPKNAAHWRPALAGMAVGEEYGSVRRLIASGQTQEAEQRLMTLARQPGQSE Sbjct 361 RYLEDAIA ADPKNAAHWRPALAGMAVGEEYGSVRRLIASGQTQEAEQRLMTLARQPGQSE 420 Query 1261 GATLMLADLQRSTGQTGEAERNYRAILARNGDNPIALMGLARVLMGEGQESEANALLSRL 1440 GATLMLADLQRSTGQTGEAERNYRAILARNGDNPIALMGLARVLMGEGQESEANALLSRL Sbjct 421 GATLMLADLQRSTGQTGEAERNYRAILARNGDNPIALMGLARVLMGEGQESEANALLSRL 480 Query 1441 GGRYSDQVQQIEVSGIMAEAARTSDSAQKVSLLRQAMTKAPDDPWLRINLANALQQQGDS 1620 GGRYSDQVQQIEVSGIMAEAARTSDSAQKVSLLRQAMTKAPDDPWLRINLANALQQQGDS Sbjct 481 GGRYSDQVQQIEVSGIMAEAARTSDSAQKVSLLRQAMTKAPDDPWLRINLANALQQQGDS 540 Query 1621 AEAANVMRPLLTSPRTPADYQAAILYASGNGNDTLARRLLAGLSPDDYSPAIRTIADEMA 1800 AEAANVMRPLLTSPRTPADYQAAILYASGNGNDTLARRLLAGLSPDDYSPAIRTIADEMA Sbjct 541 AEAANVMRPLLTSPRTPADYQAAILYASGNGNDTLARRLLAGLSPDDYSPAIRTIADEMA 600 Query 1801 IKADLASRLSMVSNPTPLVREALAAPDPTGARGVAVADLFRQRGDMLHAHMALRIASTRN 1980 IKADLASRLSMVSNPTPLVREALAAPDPTGARGVAVADLFRQRGDMLHAHMALRIASTRN Sbjct 601 IKADLASRLSMVSNPTPLVREALAAPDPTGARGVAVADLFRQRGDMLHAHMALRIASTRN 660 Query 1981 IDLTTEQRLAYATEYMKISNPVAAARLLAPLGDGSGTATGSAMSPDQRQTLMQLRMGISV 2160 IDLTTEQRLAYATEYMKISNPVAAARLLAPLGDGSGTATGSAMSPDQRQTLMQLRMGISV Sbjct 661 IDLTTEQRLAYATEYMKISNPVAAARLLAPLGDGSGTATGSAMSPDQRQTLMQLRMGISV 720 Query 2161 AQSDLLNQRGDQAAAYDHLAPALQADPEATSPKLALARLYNGRGKYGHALDIDLAVLRHN 2340 AQSDLLNQRGDQAAAYDHLAPALQADPEATSPKLALARLYNGRGKYGHALDIDLAVLRHN Sbjct 721 AQSDLLNQRGDQAAAYDHLAPALQADPEATSPKLALARLYNGRGKYGHALDIDLAVLRHN 780 Query 2341 PqdldarqaavqaaandGKDNLAMQLAQDGVQQSPMDARSWLGMAVADRAVGHGDRTLAD 2520 PQDLDARQAAVQAAANDGKDNLAMQLAQDGVQQSPMDARSWLGMAVADRAVGHGDRTLAD Sbjct 781 PQDLDARQAAVQAAANDGKDNLAMQLAQDGVQQSPMDARSWLGMAVADRAVGHGDRTLAD 840 Query 2521 LRRAYELRLQQLKISRGDAIGGDETQATAPPTANPFRRDAYGHALSLGAPPGENGYSTAG 2700 LRRAYELRLQQLKISRGDAIGGDETQATAPPTANPFRRDAYGHALSLGAPPGENGYSTAG Sbjct 841 LRRAYELRLQQLKISRGDAIGGDETQATAPPTANPFRRDAYGHALSLGAPPGENGYSTAG 900 Query 2701 SVPEISDQMLSSINGQIHTLSEDMAPSVDAGLGFRVRSGTPGMGALTEASVPIVGRIPLQ 2880 SVPEISDQMLSSINGQIHTLSEDMAPSVDAGLGFRVRSGTPGMGALTEASVPIVGRIPLQ Sbjct 901 SVPEISDQMLSSINGQIHTLSEDMAPSVDAGLGFRVRSGTPGMGALTEASVPIVGRIPLQ 960 Query 2881 AgtsaltftatptfltsgHLPQTGYDIPRFGTNLFALERNLQNQNNSAEHRINTDTIGRE 3060 AGTSALTFTATPTFLTSGHLPQTGYDIPRFGTNLFALERNLQNQNNSAEHRINTDTIGRE Sbjct 961 AGTSALTFTATPTFLTSGHLPQTGYDIPRFGTNLFALERNLQNQNNSAEHRINTDTIGRE 1020 Query 3061 AGVAPDVRFANNWVSADVGASPLGFTLPNVIGGVEFAPRVGPVTFRVSGERRSITNSVLS 3240 AGVAPDVRFANNWVSADVGASPLGFTLPNVIGGVEFAPRVGPVTFRVSGERRSITNSVLS Sbjct 1021 AGVAPDVRFANNWVSADVGASPLGFTLPNVIGGVEFAPRVGPVTFRVSGERRSITNSVLS 1080 Query 3241 YGGMTDALTGKKWGGVVTNHFHGQVEATLGNTIVYGGGGYAIQTGHHVQSNTEVEGGLGA 3420 YGGMTDALTGKKWGGVVTNHFHGQVEATLGNTIVYGGGGYAIQTGHHVQSNTEVEGGLGA Sbjct 1081 YGGMTDALTGKKWGGVVTNHFHGQVEATLGNTIVYGGGGYAIQTGHHVQSNTEVEGGLGA 1140 Query 3421 NTLVYRNRKHEVRVGVNLTYFGYKHNEDFYTYGQGGYFSPQSYFAATVPVRYSGHSGLFD 3600 NTLVYRNRKHEVRVGVNLTYFGYKHNEDFYTYGQGGYFSPQSYFAATVPVRYSGHSGLFD Sbjct 1141 NTLVYRNRKHEVRVGVNLTYFGYKHNEDFYTYGQGGYFSPQSYFAATVPVRYSGHSGLFD 1200 Query 3601 WDVTGSIGYQLFHEHSSAFFPTNPVYQALANGLAGVSTAELSLESARYPGDDVGSLVGGF 3780 WDVTGSIGYQLFHEHSSAFFPTNPVYQALANGLAGVSTAELSLESARYPGDDVGSLVGGF Sbjct 1201 WDVTGSIGYQLFHEHSSAFFPTNPVYQALANGLAGVSTAELSLESARYPGDDVGSLVGGF 1260 Query 3781 DGRVGYRVSHSLRLDLSGRFQKAGNWDEGGAMISAHYLIMDQ 3906 DGRVGYRVSHSLRLDLSGRFQKAGNWDEGGAMISAHYLIMDQ Sbjct 1261 DGRVGYRVSHSLRLDLSGRFQKAGNWDEGGAMISAHYLIMDQ 1302 Lu teaches a motivation to synthesize cellulose from A. xylinum in plants for textile fibers, because bacterial cellulose has useful properties such as fine structure and high Young’s modulus suitable for industrial application (page 441, left column, paragraph 1-right column, paragraph 1). Lu teaches that producing bacterial cellulose in cotton might enhance fiber strength, fineness, and useful mechanical properties (page 441, right column, paragraph 2-page 442, left column, paragraph 1). Lu teaches that the cellulose synthesizing operon from A. xylinum comprises acsA, acsB, acsC, and acsD genes and the gene acsA was named for A. xylinum strain ATCC 53582 (page 441, right column, paragraph 2). Lu teaches a method of amplifying the genes from A. xylinum DNA and cloning the genes into a pBluescript SK vector (page 442, left column, paragraph 2-right column, paragraph 5). After ligation of the genes with CaMV35S, Lu teaches the acsA and acsB genes cloned into pCAMBIA1301 and acsC and acsD genes cloned into pCOB302-3 (page 442 right column, paragraph 5; figure 4). pCOB302-3-C+D and pCAMBIA-A + B are plant transformation vectors (page 443, left column, paragraph 3). Lu teaches each gene linked to a promoter and a 3’ element; acsD is linked to a NOS 3’ element (figure 4). Transgenic cotton plants were produced via the pollen-tube-pathway comprising these vectors (page 443, left column, paragraph 4-right column, paragraph 1). Mozes-Koch teaches a method of transforming plants with a bacterial prn operon to produce an antifungal, antibacterial compound (page 18866, left column, paragraph 1-right column, paragraph 3). Mozes-Koch teaches a motivation to express an entire bacterial operon in plants for metabolic engineering because the clustering of genes together indicates possibility of preserving clusters upon meiosis and recombination and may result in coregulation (page 1883, left column, paragraph 1-right column, paragraph 2). Mozes-Koch teaches that the I operon is rather large (page 1888, right column, paragraph 2). Reddy teaches that rice straw is one of the most common sources of biomass available in the world and is cheaper and more environmentally friendly than cotton fiber because cotton consumes >25% of insecticide used in the world (page 8077, left column, paragraphs 1-2). Reddy teaches a motivation to use rice straw for fiber to replace environmentally unfriendly natural fibers currently in use (page 8081, left column, paragraph 2). Kawabe teaches that there is a positive correlation between codon usage and gene expression in plants transformed with vectors expressing genes with modified codon usage (page 343, left column, paragraph 2). Kawabe summarizes differences in codon usage among plant species, including rice (table 1) and teaches preferred codons in different species, including rice (table 2). Before the filing date of the instant application, it would have been obvious to one of ordinary skill in the art to modify the method of Akhtar and Lu to transform a cotton plant with transformation vectors comprising acsA, acsB, acsC, and acsD genes to use a single acs operon comprising acsAB, acsC and acsD genes. One of ordinary skill in the art would have been motivated to use a single nucleic acid molecule comprising all genes in the same orientation, as is found naturally in Gluconacetobacter hansenii, because a single nucleic acid molecule, as in an operon, allows for cosegregation of the genes. One of ordinary skill in the art would have had reasonable expectation of success in using a single transformation vector comprising all genes, because transformation of plants with a single vector comprising multiple transgenes was known in the art prior to the filing of the instant application. Furthermore, one of ordinary skill in the art would have had reasonable expectation of success in transforming and expressing acsAB, acsC and acsD genes, because Akhtar’s method of expressing just the transgenes of acsA and acsB in cotton led to increased cellulose in cotton fibers and enhanced cellulose quality. Before the filing date of the instant application, it would have been obvious to one of ordinary skill in the art to use an acsAB and an acsD gene identical to those found in GenBank sequence AB091060.1, in which the acsAB, acsC, and acsD genes are all present sequentially and in the same orientation within GenBank sequence AB091060.1, which reads on tandemly in a same orientation. Likewise, it would have been obvious to one of ordinary skill in the art to use an acsC gene identical to the acsC gene of A. xylinum ATCC 53582 as taught by GenBank sequence X54676.1. One of ordinary skill in the art would have been motivated to test different known gene sequences encoding the acsAB, acsC, and acsD genes as part of design choice. One of ordinary skill in the art would have had reasonable expectation of success, because acsAB, acsC, and acsD genes encoding amino acid sequences with 100% sequence identity to the proteins encoded by instant SEQ ID NOs: 2, 4 & 6 were known in the art prior to the filing of the instant specification. One of ordinary skill in the art would have expected known acsAB, acsC, and acsD genes to function similarly to other bacterial cellulose A, B, C, and D genes. Before the filing date of the instant application, it would have been obvious to one of ordinary skill in the art to operably link each gene with a 35S promoter and a NOS terminator. The transformation vectors of Lu teach each acs gene operably linked individually to a promoter and a 3’ region, including a 35S promoter and a Nos 3’ element (figure 4). Choosing to link each gene with a 35S promoter and a NOS terminator rather than a variety of promoters and terminators would have been a design choice, well within the bounds of vector design which would have been routine to one of ordinary skill in the art prior to the filing of the instant application. Finally, before the filing date of the instant application, it would have been obvious to one of ordinary skill in the art to apply the method of transformation taught by Lu to transform rice. One of ordinary skill in the art would have been motivated to transform rice instead of cotton, because rice straw is one of the most common sources of biomass and would provide an environmentally friendly alternative to cotton. One of ordinary skill in the art would have had reasonable expectation of success because transformation of rice plants was routine prior to the filing of the instant application. In transforming a rice plant, it would have been obvious to use codon-optimized nucleic acid sequences of SEQ ID NO: 2, 4, and 6, which encode sequences identical to the proteins encoded by the acsAB and acsD genes of GenBank sequence AB091060.1 and the acsC gene of GenBank sequence X54676.1. One of ordinary skill in the art would have been motivated to codon optimize the acs genes, and apply any other sequence optimization, because plants transformed with genes with modified codon usage have a positive correlation between codon usage and gene expression. One of ordinary skill in the art would have had reasonable expectation of success, because modifying codon usage in vectors for plant transformation had been known and practiced in the art long before the filing date of the instant application. Akhtar teaches that the cellulose content of the cotton fibers increased from 258.7 μg/g in non-transgenic control plant line to 301.2-316.8 μg/g cellulose contents of transgenic cotton fibers. (301.2-258.7)/258.7 is 16.4%. It is indefinite what phenotypes are encompassed by “about 3.81% of total cellulose content”. A 16.4% increase in cellulose content of cotton fiber reads on the Broadest Reasonable Interpretation of the limitation. Thus, a plant transformation vector comprising a multi-gene expression cassette comprising three complete gene expression cassettes tandemly ligated in the same orientation, comprising a codon optimized acsAB gene of SEQ ID NO: 2, a codon-optimized acsC gene of SEQ ID NO: 4, and a codon-optimized acsD gene of SEQ ID NO: 6, each operably linked to a 35S promoter and a NOS terminator, would have been obvious to one of skill in the art, and transforming the plant transformation into a rice plant would have been obvious. Applicant urges that there is not a reasonable expectation that combining known biological tools would achieve the specific, complex functional result claimed in the instant invention. Applicant urges that because the claims now require the vector, when transformed into a rice plant, to produce bacterial cellulose at a content of about 3.81% of total cellulose content, and the prior art does not provide a reasonable expectation that such a vector would achieve functional bacterial cellulose production at the claimed level in rice (Remarks, page 6, paragraph 3). This argument is unpersuasive, because the specification does not define “about 3.81%” in such a way that patentably distinguishes the claimed result from prior art, which teaches an increase in cellulose from the expression of bacterial cellulose transgenes in plants. Because the prior art teaches an increase in cellulose from the expression of bacterial cellulose transgenes, one of ordinary skill in the art would have had a reasonable expectation of success in combining known biological tools to achieve a result similar to that taught by the prior art. Applicant urges that Lu did not demonstrate functional expression of the bacterial cellulose genes and so the absence of reported bacterial cellulose production in Lu’s cotton transformants underscores the gap between gene delivery and functional assembly (Remarks, page 7, paragraph 1; Affidavit, paragraph 8). This argument is unpersuasive, because Akhtar describes modified cellulose production in cotton as a result of introduced bacterial cellulose genes, including an increase in total cellulose and increased fiber quality. Akhtar’s measurements read on the instant specification’s description of functional expression of the bacterial cellulose transgenes based on total cellulose content and improved rice straw quality (paragraph [0091], table 1). The prior art teaches that the functional expression of bacterial cellulose genes is enabled in plants. Furthermore, Lu's silence regarding the expression of bacterial cellulose does not make expression of bacterial cellulose in plants an unexpected result. Applicant urges that because a soluble metabolite pathway is fundamentally biologically distinct from a membrane-associated multi-protein complex, Mozes-Koch’s teachings of a motivation to use a tandem expression vector for transgenes conflates motivation with reasonable expectation of success. Applicant urges that acs proteins must localize to the plasma membrane, insert correctly, and assemble into a functional complex to polymerize and secrete cellulose. Applicant urges that heterologous expression of bacterial membrane protein complexes in plants is difficult and unpredictable due to differences in membrane composition, protein folding machinery, and ER quality control. Applicant urges that Mozes-Koch’s success with a soluble pathway in a dicot host provides no reasonable expectation of success for a membrane complex in a monocot (Remarks, page 7, paragraph 2). This argument is unpersuasive, because Akhtar teaches expression of bacterial cellulose proteins in plants, which resulted in modified and increased cellulose expression in plants, which provides a reasonable expectation of success. Mozes-Koch’s teachings provide evidence that a tandem expression vector of multiple transgenes for expression in a plant was obvious prior to the filing of the instant application. Based on Mozes-Koch, one of skill in the art would have had reasonable expectation of success in using a single, tandem vector rather than two separate vectors with differently oriented gene expression cassettes (as Lu used). Localization, insertion, and assembly of proteins, and membrane composition, protein folding, and ER quality control, do not relate to the enablement of transformation and transcription of a tandemly ligated expression vector, which is the teaching of Mozes-Koch relied on for the instant rejection. Applicant urges that a POSITA would have recognized the biological distinction between soluble prn metabolic pathway and membrane bound acsABCD complex because soluble enzymes in the cytosol face different challenges than membrane proteins, so success with a soluble pathway in a dicot provides no meaningful guidance for expressing a bacterial membrane complex in a monocot (Affadavit paragraph 9). This argument is unpersuasive, because Mozes-Koch was relied on in the instant rejection for the motivation to transform a plant with transgenes on the same vector: to achieve cosegregation of transgenes. As Applicant describes in paragraph 8 of the Affidavit, DNA delivery is distinct from functional expression. Applicant's argument that Mozes-Koch teaches a system with a different functional expression than the instant application is irrelevant to the teachings regarding DNA delivery and Mozes-Koch's motivation to use a single vector for DNA delivery of multiple genes. Applicant urges that the instant invention overcomes a technical prejudice that would have discouraged a POSITA from pursing the claimed approach, because reconstructing a bacterial membrane complex in a plant would require precise stoichiometric regulation of subunit expression, and conventional wisdom held that differential promoter strengths or other regulatory mechanisms were necessary to balance expression of the genes (Remarks, page 7, paragraph 3-page 8, paragraph 1; affidavit paragraph 10). This argument is unpersuasive, because selection of any one known promoter or terminator over another can be considered a design choice in the use of a transformation vector. Testing different combinations of regulatory elements in a transformation vector would have been routine and well within the ordinary level of skill in the art of plant biotechnology. Applicant has not provided any data to demonstrate that Applicant's vector design, using a 35S promoter and NOS terminator for each transgene, provided unexpectedly beneficial results over another combination of promoters and terminators. Applicant does not provide evidence comparing the vector of the instant claims to the hypothetical design Applicant urges a POSITA would have used. If Applicant's design does not provide unexpectedly better results than the combination of regulatory mechanisms that would have been obvious to a POSITA, then the vector of the instant Application cannot be considered non-obvious, given the known motivations to deliver multi-gene transgenic constructs to plants. Applicant urges that the specification demonstrates that the claimed sequences in the claimed arrangement represents the first successful demonstration of bacterial cellulose synthesis in a plant system, and that this was unexpected (page 8, paragraph 2; affidavit paragraph 11). This argument is unpersuasive, because Akhtar teaches expression of transgenes encoding proteins involved in bacterial cellulose in cotton (page 6, left column, paragraph 3) and significantly increased cellulose in the fibers of the transgenic plant (page 7, left column, paragraph 1-right column; figure 7). Proteins involved in bacterial cellulose synthase had been successfully expressed in plants and were capable of functioning in the production of cellulose. Applicant's production of a bacterial cellulose synthase complex in a plant would not be considered an unexpected result in light of the prior art teachings of bacterial cellulose synthase complex genes functioning in plants. Conclusion No claims are allowed. Any inquiry concerning this communication or earlier communications from the examiner should be directed to Victoria L DeLeo whose telephone number is (703)756-5998. The examiner can normally be reached M-F 8:00am-4pm EDT. 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Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /VICTORIA L DELEO/Examiner, Art Unit 1662 /Anne Kubelik/Primary Examiner, Art Unit 1663