Prosecution Insights
Last updated: April 19, 2026
Application No. 18/628,166

Anomalous Fragment Detection and Classification

Non-Final OA §101§112§DP
Filed
Apr 05, 2024
Examiner
LIU, GUOZHEN
Art Unit
1686
Tech Center
1600 — Biotechnology & Organic Chemistry
Assignee
Grail, Inc.
OA Round
1 (Non-Final)
50%
Grant Probability
Moderate
1-2
OA Rounds
4y 8m
To Grant
75%
With Interview

Examiner Intelligence

Grants 50% of resolved cases
50%
Career Allow Rate
47 granted / 95 resolved
-10.5% vs TC avg
Strong +25% interview lift
Without
With
+25.4%
Interview Lift
resolved cases with interview
Typical timeline
4y 8m
Avg Prosecution
39 currently pending
Career history
134
Total Applications
across all art units

Statute-Specific Performance

§101
37.1%
-2.9% vs TC avg
§103
25.2%
-14.8% vs TC avg
§102
7.3%
-32.7% vs TC avg
§112
19.8%
-20.2% vs TC avg
Black line = Tech Center average estimate • Based on career data from 95 resolved cases

Office Action

§101 §112 §DP
DETAILED ACTION Notice of Pre-AIA or AIA Status The present application, filed on or after March 16, 2013, is being examined under the first inventor to file provisions of the AIA . The IDS filed 07/03/2024 has been considered by the Examiner. Consequently a 1449 form is attached. Priority Applicant’s claim for the benefit of a prior-filed application under 35 U.S.C. 119(e) or under 35 U.S.C. 120, 121, or 365(c) is acknowledged. Priority of US application 62/642,480 filed 03/13/2018 is acknowledged. Claim Rejections - 35 USC § 112 The following is a quotation of 35 U.S.C. 112(b): (b) CONCLUSION.—The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the inventor or a joint inventor regards as the invention. The following is a quotation of 35 U.S.C. 112 (pre-AIA ), second paragraph: The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the applicant regards as his invention. Claim 21 is rejected under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA ), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention. Claim 21 recites “wherein applying the cancer classifier to the sample state vector generates at least one of a cancer probability and a non-cancer probability”. However, in claim 12 last step, the “cancer classifier” is applied to “the feature vector”, not the “sample state vector”. It becomes unclear in claim 21, which vector is applied by the cancer classifier, or “the feature vector” and “the sample state vector” are the same, or they are interchangeable. To advance compact prosecution, a “feature vector” is interpretable as one “sample state vector” for the following reason: According to instant claim 2 limitation “generating a feature vector from one or more sample state vectors for sample fragments determined to have an anomalous methylation pattern”, literally, “a feature vector” is “one sample state vector” under a BRI. In reality, there is only one sample state vector for one sample fragment because there is only one “sample state vector” for one sample fragment according to this limitation: “generating a sample state vector for the sample fragment comprising a sample genomic location within the reference genome and a methylation state for each of a plurality of CpG sites in the sample fragment” (claim 2 step 2). Hence, without disclosing any “feature” other than the methylation state and probability/possibility disclosed for the “sample state vector”, a “feature vector” is a “sample state vector” under a BRI, or at least. “one sample state vector” in the reference claim 1 anticipate “a feature vector” in the instant claim 2. Claim Rejections - 35 USC § 101 35 U.S.C. 101 reads as follows: Whoever invents or discovers any new and useful process, machine, manufacture, or composition of matter, or any new and useful improvement thereof, may obtain a patent therefor, subject to the conditions and requirements of this title. Claims 2-21 are rejected under 35 U.S.C. 101 because the claimed invention is directed to non-statutory subject matter. Step 1: Process, Machine, Manufacture or Composition Claims 2-11 are directed to a process, here a “method” for detecting cancer in a test subject from a cell-free deoxyribonucleic acid (cfDNA) sample, with a series functional steps. Claims 12-21 is directed to a machine or manufacturer, here a “non-transitory computer-readable storage medium” storing instructions for detecting cancer in a test subject from a cell-free deoxyribonucleic acid (cfDNA) sample fragment, with a well-known structural component. Step 2A Prong One: Identification of an Abstract Idea The claims recite: Generating a list of possibilities of a methylation state vector from the sample genomic location that are of a same length as the sample state vector, wherein each possibility of the methylation state vector is distinct from other possibilities of the methylation state vector (claims 2 and 12); --This step recites generating a list of possibilities of a methylation state vector from the sample genomic location that are of a same length as the sample state vector. The step is interpreted as initializing a methylation state vector with control possibility for CpG sites in the vector (of a fragment). According to the disclosure at paragraph [0005], this step encompasses a control group data and a Markov chain probability analysis. The step hence recites mathematical calculations implicitly and hence it equates to an abstract idea of mathematical concepts. For each possibility of the methylation state vector, calculating a probability by accessing the counts stored in the data structure (claims 2 and 12); --This step recites calculation a probability by accessing the counts stored in the data structure. The step recites a mathematical calculation explicitly and hence it equates to an abstract idea of mathematical concepts. Identifying the possibility of the methylation state vector that matches the sample state vector and the calculated probability associated with the identified possibility (claims 2 and 12); --This step recites identifying the possibilities of the methylation state vector that matches the sample state vector and the calculated probability associated with the identified possibility. Under a BRI, this step encompasses the processes of comparing two vectors and identifying associated values from one vector. This processes can be achieved in human mind. Hence this step equates to an abstract idea of mental processes. Generating a score for the sample fragment of the sample state vector based at least in part on the calculated probability for the identified possibility (claims 2 and 12); --This step recites calculation a score for the sample fragment of the sample state vector based at least in part on the calculated probability for the identified possibility. The step recites mathematical calculations explicitly and hence it equates to an abstract idea of mathematical concepts. Determining whether the sample fragment has an anomalous methylation pattern based on the generated score (claims 2 and 12); --This step recites a decision making/judgement activity, which equates to an abstract idea of mental process. Generating a feature vector from one or more sample state vectors for sample fragments determined to have an anomalous methylation pattern (claims 2 and 12); --This step recites generation new data (a feature vector) from existing data (one or more sample state vectors for sample fragments determined to have an anomalous methylation pattern), which is a data manipulation and equates to an abstract idea of mental process. Applying a cancer classifier to the feature vector to determine a cancer prediction for the test subject (claims 2 and 12); --Although recited as determined by a cancer classifier, nothing would prevent the human mind from determining the cancer or cancer status in the test subject. Therefore, this step equates to an abstract idea of mental processes. Step 2A Prong Two: Consideration of Practical Application The claims result in a process of predicting cancer using the feature vector as input, which reads on generating new data (aka information) from existing data. The claims do not recite any additional elements that integrate the abstract idea/judicial exception into a practical application. This judicial exception is not integrated into a practical application because the claims do not meet any of the following criteria: An additional element reflects an improvement in the functioning of a computer, or an improvement to other technology or technical field; an additional element that applies or uses a judicial exception to effect a particular treatment or prophylaxis for a disease or medical condition; an additional element implements a judicial exception with, or uses a judicial exception in conjunction with, a particular machine or manufacture that is integral to the claim; an additional element effects a transformation or reduction of a particular article to a different state or thing; and an additional element applies or uses the judicial exception in some other meaningful way beyond generally linking the use of the judicial exception to a particular technological environment, such that the claim as a whole is more than a drafting effort designed to monopolize the exception. Step 2B: Consideration of Additional Elements and Significantly More The claimed method also recites "additional elements" that are not limitations drawn to an abstract idea. The recited additional elements are drawn to: Accessing a data structure comprising counts of strings of a plurality of CpG sites within a reference genome (claims 2 and 12); A non-transitory computer-readable storage medium (claim 12). The claims do not include additional elements that are sufficient to amount of significantly more than the judicial exception because it is routine and conventional to perform the acts of predicting cancer using CpG methylation information. “Accessing a data structure …” (claims 2 and 12) is insignificant extra-solution activity of data-gathering. Reciting the computer storage medium (claim 12) is a mere application of abstract idea in a computer environment (MPEP § 2106.05(h)). The conventionality of recited additional elements are further evidenced by the following references: Kang (“CancerLocator: non-invasive cancer diagnosis and tissue-of-origin prediction using methylation profiles of cell-free DNA”. Genome Biol 18, 53 (2017), as cited on the "Notice of References Cited" form 892 0f 4/19/2022. Newly cited); Shen (“Detect differentially methylated regions using non-homogeneous hidden Markov model for methylation array data”, Bioinformatics, Volume 33, Issue 23, 01 December 2017, Pages 3701–3708. Newly cited); and Krueger (“Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications”, Bioinformatics, Volume 27, Issue 11, 1 June 2011, Pages 1571–1572, (Year: 2011), Fig 1, page 1572. Newly cited). Kang discloses a computational method to locate cancer origin (para 2, col 1, pg. 7). Kang further teaches accessing a data structure comprising counts of strings of CpG sites within a reference genome and their respective methylation states from a set of training fragments (Fig. 1, page 3 of 12); and generating of a sample state vector for each CpG cluster (Fig 1 upper part, page 3 of 12; " the numbers of methylated and unmethylated cytosines are counted for each CpG site” (line 3-5, par 3 col 1, page 8 of 12). Shen discloses the DMRMark, a novel method based on non-homogeneous hidden Markov model (NHMM) to detect DMRs from methylation array data (DMR stands for “Differentially Methylation Regions”, examiner) (page 3702-3704 under section “2 Model”, and Fig 2). Krueger’s Bismark is employed to align the reads to the reference genome HG19 and call the methylated cytosines (“Bismark is employed to align the reads to the reference genome HG19 and call the methylated cytosines”, first 2 lines under section “WGBS data processing”, col 1, page 8 of 12). It is emphasized that, analysis of what is conventional generally pertains to the above-identified additional elements and not to elements earlier identified as part of a judicial exception. Other elements of the method include a non-transitory computer-readable storage medium which is a recitation of generic computer structure that serves to perform generic computer functions that are well-understood, routine, and conventional activities previously known to the pertinent industry. Viewed as a whole, these additional claim element(s) do not provide meaningful limitation(s) to transform the abstract idea recited in the instantly presented claims into a patent eligible application of the abstract idea such that the claim(s) amounts to significantly more than the abstract idea itself. Therefore, the claim(s) are rejected under 35 U.S.C. 101 as being directed to non-statutory subject matter. Double Patenting The nonstatutory double patenting rejection is based on a judicially created doctrine grounded in public policy (a policy reflected in the statute) so as to prevent the unjustified or improper timewise extension of the “right to exclude” granted by a patent and to prevent possible harassment by multiple assignees. A nonstatutory double patenting rejection is appropriate where the conflicting claims are not identical, but at least one examined application claim is not patentably distinct from the reference claim(s) because the examined application claim is either anticipated by, or would have been obvious over, the reference claim(s). See, e.g., In re Berg, 140 F.3d 1428, 46 USPQ2d 1226 (Fed. Cir. 1998); In re Goodman, 11 F.3d 1046, 29 USPQ2d 2010 (Fed. Cir. 1993); In re Longi, 759 F.2d 887, 225 USPQ 645 (Fed. Cir. 1985); In re Van Ornum, 686 F.2d 937, 214 USPQ 761 (CCPA 1982); In re Vogel, 422 F.2d 438, 164 USPQ 619 (CCPA 1970); In re Thorington, 418 F.2d 528, 163 USPQ 644 (CCPA 1969). A timely filed terminal disclaimer in compliance with 37 CFR 1.321(c) or 1.321(d) may be used to overcome an actual or provisional rejection based on nonstatutory double patenting provided the reference application or patent either is shown to be commonly owned with the examined application, or claims an invention made as a result of activities undertaken within the scope of a joint research agreement. See MPEP § 717.02 for applications subject to examination under the first inventor to file provisions of the AIA as explained in MPEP § 2159. See MPEP § 2146 et seq. for applications not subject to examination under the first inventor to file provisions of the AIA . A terminal disclaimer must be signed in compliance with 37 CFR 1.321(b). The filing of a terminal disclaimer by itself is not a complete reply to a nonstatutory double patenting (NSDP) rejection. A complete reply requires that the terminal disclaimer be accompanied by a reply requesting reconsideration of the prior Office action. Even where the NSDP rejection is provisional the reply must be complete. See MPEP § 804, subsection I.B.1. For a reply to a non-final Office action, see 37 CFR 1.111(a). For a reply to final Office action, see 37 CFR 1.113(c). A request for reconsideration while not provided for in 37 CFR 1.113(c) may be filed after final for consideration. See MPEP §§ 706.07(e) and 714.13. The USPTO Internet website contains terminal disclaimer forms which may be used. Please visit www.uspto.gov/patent/patents-forms. The actual filing date of the application in which the form is filed determines what form (e.g., PTO/SB/25, PTO/SB/26, PTO/AIA /25, or PTO/AIA /26) should be used. A web-based eTerminal Disclaimer may be filled out completely online using web-screens. An eTerminal Disclaimer that meets all requirements is auto-processed and approved immediately upon submission. For more information about eTerminal Disclaimers, refer to www.uspto.gov/patents/apply/applying-online/eterminal-disclaimer. Claims 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21 are rejected on the ground of nonstatutory double patenting as being unpatentable over claims 1, 12, 2, 14, 5, 6, 7, 8, 11, 12, 12, 14, 15, 16, 17, 18, 19, 22 of U.S. Patent No. US12027237B2. Although the claims at issue are not identical, they are not patentably distinct from each other because every step of instant claim 2 is claimed in the reference claim 1, sometimes in more details. The order of the instant steps are different comparing the order of the same steps in the reference claim 1 but every steps in the instant claim 2 is anticipated by at least one step in the reference claim 1. Instant claim 2 fed “a feature vector” to a cancer classifier to determine a cancer prediction. In the reference claim 1, it is the “sample state vector” that applied by a cancer classifier to determine a cancer prediction. According to instant claim 2 limitation “generating a feature vector from one or more sample state vectors for sample fragments determined to have an anomalous methylation pattern”, Literally, “a feature vector” is “one sample state vector” under a BRI. In reality, there is only one sample state vector for one sample fragment because there is only one “sample state vector” for one sample fragment according to this limitation: “generating a sample state vector for the sample fragment comprising a sample genomic location within the reference genome and a methylation state for each of a plurality of CpG sites in the sample fragment” (claim 2 step 2). Hence, without disclosing any “feature” other than the methylation state and probability/possibility disclosed for the “sample state vector”, a “feature vector” is a “sample state vector” under a BRI, or at least. “one sample state vector” in the reference claim 1 anticipate “a feature vector” in the instant claim 2. In summary, Claims 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21 are rejected on the ground of nonstatutory double patenting as being unpatentable over claims 1, 12, 2, 14, 5, 6, 7, 8, 11, 12, 12, 14, 15, 16, 17, 18, 19, 22 of U.S. Patent No. US12027237B2. Conclusion No claims are allowed. Any inquiry concerning this communication or earlier communications from the examiner should be directed to GUOZHEN LIU whose telephone number is (571)272-0224. The examiner can normally be reached Monday-Friday 8-5. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Larry D Riggs can be reached at (571) 270-3062. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /GL/ Patent Examiner Art Unit 1686 /Anna Skibinsky/ Primary Examiner, AU 1635
Read full office action

Prosecution Timeline

Apr 05, 2024
Application Filed
Jan 24, 2026
Non-Final Rejection — §101, §112, §DP (current)

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Study what changed to get past this examiner. Based on 5 most recent grants.

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Prosecution Projections

1-2
Expected OA Rounds
50%
Grant Probability
75%
With Interview (+25.4%)
4y 8m
Median Time to Grant
Low
PTA Risk
Based on 95 resolved cases by this examiner. Grant probability derived from career allow rate.

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