Prosecution Insights
Last updated: April 19, 2026
Application No. 18/657,340

CITRULLINATED BRAIN AND NEUROLOGICAL PROTEINS AS BIOMARKERS OF BRAIN INJURY OR NEURODEGENERATION

Final Rejection §101§112
Filed
May 07, 2024
Examiner
CHERNYSHEV, OLGA N
Art Unit
1675
Tech Center
1600 — Biotechnology & Organic Chemistry
Assignee
The Johns Hopkins University
OA Round
2 (Final)
54%
Grant Probability
Moderate
3-4
OA Rounds
3y 2m
To Grant
89%
With Interview

Examiner Intelligence

Grants 54% of resolved cases
54%
Career Allow Rate
512 granted / 942 resolved
-5.6% vs TC avg
Strong +34% interview lift
Without
With
+34.4%
Interview Lift
resolved cases with interview
Typical timeline
3y 2m
Avg Prosecution
44 currently pending
Career history
986
Total Applications
across all art units

Statute-Specific Performance

§101
14.8%
-25.2% vs TC avg
§103
8.6%
-31.4% vs TC avg
§102
12.6%
-27.4% vs TC avg
§112
45.6%
+5.6% vs TC avg
Black line = Tech Center average estimate • Based on career data from 942 resolved cases

Office Action

§101 §112
Notice of Pre-AIA or AIA Status The present application is being examined under the pre-AIA first to invent provisions. DETAILED ACTION Response to Amendment 1. Claims 2, 5 and 8-10 has been amended, claim 4 canceled and claim 21 added as requested in the amendment filed on February 04, 2026. Following the amendment, claims 2, 3 and 5-21 are pending in the instant application. 2. Claims 11-20 stand withdrawn from further consideration pursuant to 37 CFR 1.142(b) as being drawn to a nonelected invention(s), there being no allowable generic or linking claim. Election was made without traverse in the reply filed on August 13, 2025. 3. Claims 2, 3, 5-10 and 21 are under examination in the instant office action. 4. Any objection or rejection of record, which is not expressly repeated in this action has been overcome by Applicant’s response and withdrawn. 5. Applicant’s arguments filed on February 04, 2026 have been fully considered but found to be not persuasive for reasons set forth below. New grounds of rejection necessitated by Applicant’s amendment are set forth below as well. Claim Rejections - 35 USC § 112 The following is a quotation of 35 U.S.C. 112(b): (b) CONCLUSION.—The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the inventor or a joint inventor regards as the invention. The following is a quotation of 35 U.S.C. 112 (pre-AIA ), second paragraph: The specification shall conclude with one or more claims particularly pointing out and distinctly claiming the subject matter which the applicant regards as his invention. 6. Claims 2, 3, 5-10, as amended, and new claims 21 are rejected under 35 U.S.C. 112(b) or 35 U.S.C. 112 (pre-AIA ), second paragraph, as being indefinite for failing to particularly point out and distinctly claim the subject matter which the inventor or a joint inventor (or for applications subject to pre-AIA 35 U.S.C. 112, the applicant), regards as the invention. 7. Claim 2, as currently presented, is vague and ambiguous for reasons that follow. The claim is drawn to a process—method for detecting brain injury biomarker proteins having post-translational modification. The process comprises three steps, (a) to detect GFAP, MBP or NRGN protein, (b) to subject the biofluid sample containing GFAP, MBP or NRGN to a detection method, and (c) to detect post-translationally modification of GFAP, MBP or NRGN. It is not obvious and cannot be determined from the claim or the specification as filed what specific physical step differentiates steps (a) and (b). This renders the claim indefinite. 8. Claim 9, as amended, recites the limitation "administering brain injury therapy" in claim 2. There is insufficient antecedent basis for this limitation in the claim. 9. Claims 9 and 10, as amended, are vague and indefinite for reciting limitations “levels […] that trend toward [or away from] levels.” The metes and bounds of the limitations cannot be determined from the claim or the specification as filed. 10. Also, claim 10, as amended, recites the limitation "non-brain injury control," which lacks definition within the specification and is generally ambiguous. For instance, is injury to a leg considered a “non-brain injury?” 11. Claims 3, 5-8 and 21 are indefinite for being dependent from indefinite claim(s). Claim Rejections - 35 USC § 101 35 U.S.C. 101 reads as follows: Whoever invents or discovers any new and useful process, machine, manufacture, or composition of matter, or any new and useful improvement thereof, may obtain a patent therefor, subject to the conditions and requirements of this title. 12. Claim 9 stands rejected under 35 U.S.C. 101 because the claimed invention is directed to a judicial exception (i.e., a law of nature, a natural phenomenon, or an abstract idea) without significantly more for reasons of record in section 13 of Paper mailed on September 05, 2025. Applicant argues at pp. 15-16 of the Response that the claim has been amended to recite specific steps that are not routine and, further, because the claim depends from claim 2, which satisfies 35 U.S.C. 101, then claim 9 is eligible as well. Applicant’s arguments have been given full consideration but found to be not persuasive for reasons that follow. Claim 9, as amended, is directed to a method of detecting brain injury therapy efficacy by observing the changes in naturally occurring factors during the naturally occurring pathology of brain injury. As fully explained earlier, claim 9 does not recite any elements, or combinations of elements to ensure that the claim as a whole amounts to significantly more than the judicial exception because the active steps of the claim—detecting proteins and measuring post-translational modification of markers—represent routine steps that are recited at a high level of generality and encompass well-understood and purely conventional routine techniques in the art (see base claim 2, for example, describing all known assays). Furthermore, steps of evaluating changes in the levels of biomarkers during active therapy read on data collecting and observing body reaction in response to treatment, the process that occurs naturally and without any knowledge or action by Applicant. The instant situation is similar to the one in Mayo, wherein the claims recited method of administering drugs to a patient and measuring the levels of certain metabolites in the blood, wherein the level of metabolites indicates whether to adjust the dosage. The Supreme Court held that the claims recited a natural law and did not “include any “additional features that provide practical assurance that the process is more than a drafting effort designed to monopolize the law of nature itself.” Mayo Collaborative Services v. Prometheus Laboratories, Inc., 566 U.S., 132 S. Ct. 77, 101 USPQ2d 1961 (2012). Finally, with respect to Applicant’s reference to claim 2, the base claim 2 satisfies the patentability because it does not recite the judicial exception. Claim 2 is limited to measuring proteins in a sample obtained from a subject with brain injury. In contrast, claim 9 adds additional elements that tie the levels of proteins to the pathology and thus set forth the judicial exception. For reasons of record fully explained earlier and reasons above, the rejection is maintained. Conclusion 13. No claim is allowed. 14. Applicant's amendment necessitated the new ground(s) of rejection presented in this Office action. Accordingly, THIS ACTION IS MADE FINAL. See MPEP § 706.07(a). Applicant is reminded of the extension of time policy as set forth in 37 CFR 1.136(a). A shortened statutory period for reply to this final action is set to expire THREE MONTHS from the mailing date of this action. In the event a first reply is filed within TWO MONTHS of the mailing date of this final action and the advisory action is not mailed until after the end of the THREE-MONTH shortened statutory period, then the shortened statutory period will expire on the date the advisory action is mailed, and any nonprovisional extension fee (37 CFR 1.17(a)) pursuant to 37 CFR 1.136(a) will be calculated from the mailing date of the advisory action. In no event, however, will the statutory period for reply expire later than SIX MONTHS from the mailing date of this final action. Any inquiry concerning this communication or earlier communications from the examiner should be directed to OLGA N CHERNYSHEV whose telephone number is (571)272-0870. The examiner can normally be reached 9AM to 5:30PM, Monday to Friday. Examiner interviews are available via telephone, in-person, and video conferencing using a USPTO supplied web-based collaboration tool. To schedule an interview, applicant is encouraged to use the USPTO Automated Interview Request (AIR) at http://www.uspto.gov/interviewpractice. If attempts to reach the examiner by telephone are unsuccessful, the examiner’s supervisor, Jeffrey Stucker can be reached at (571)272-0911. The fax phone number for the organization where this application or proceeding is assigned is 571-273-8300. Information regarding the status of published or unpublished applications may be obtained from Patent Center. Unpublished application information in Patent Center is available to registered users. To file and manage patent submissions in Patent Center, visit: https://patentcenter.uspto.gov. Visit https://www.uspto.gov/patents/apply/patent-center for more information about Patent Center and https://www.uspto.gov/patents/docx for information about filing in DOCX format. For additional questions, contact the Electronic Business Center (EBC) at 866-217-9197 (toll-free). If you would like assistance from a USPTO Customer Service Representative, call 800-786-9199 (IN USA OR CANADA) or 571-272-1000. /OLGA N CHERNYSHEV/Primary Examiner, Art Unit 1675 February 18, 2026
Read full office action

Prosecution Timeline

May 07, 2024
Application Filed
Sep 03, 2025
Non-Final Rejection — §101, §112
Feb 04, 2026
Response Filed
Feb 18, 2026
Final Rejection — §101, §112 (current)

Precedent Cases

Applications granted by this same examiner with similar technology

Patent 12601750
DIAGNOSING MILD COGNITIVE IMPAIRMENT (MCI), PREDICTING ALZHEIMER'S DISEASE (AD) DEMENTIA ONSET, AND SCREENING AND MONITORING AGENTS FOR TREATING MCI OR PREVENTING DEMENTIA ONSET
2y 5m to grant Granted Apr 14, 2026
Patent 12601751
PROTEIN ANTIGEN COMBINATION FOR DETECTION OF ALZHEIMER'S DISEASE AND APPLICATION THEREOF
2y 5m to grant Granted Apr 14, 2026
Patent 12596126
METHODS FOR THE PREDICTION, PROGNOSIS, AND/OR DIAGNOSIS OF AN INFLAMMATORY RESPONSE ASSOCIATED WITH SCHIZOPHRENIA
2y 5m to grant Granted Apr 07, 2026
Patent 12589098
METHODS OF TREATING PRADER WILLI SYNDROME AND CONDITIONS ASSOCIATED WITH LOW BASAL METABOLIC RATE OR HYPERPHAGIA USING A KATP CHANNEL OPENER
2y 5m to grant Granted Mar 31, 2026
Patent 12582693
GLUTATHIONE TRISULFIDE (GSSSG) IN NEUROPROTECTION
2y 5m to grant Granted Mar 24, 2026
Study what changed to get past this examiner. Based on 5 most recent grants.

AI Strategy Recommendation

Get an AI-powered prosecution strategy using examiner precedents, rejection analysis, and claim mapping.
Powered by AI — typically takes 5-10 seconds

Prosecution Projections

3-4
Expected OA Rounds
54%
Grant Probability
89%
With Interview (+34.4%)
3y 2m
Median Time to Grant
Moderate
PTA Risk
Based on 942 resolved cases by this examiner. Grant probability derived from career allow rate.

Sign in with your work email

Enter your email to receive a magic link. No password needed.

Personal email addresses (Gmail, Yahoo, etc.) are not accepted.

Free tier: 3 strategy analyses per month